Multiscale Topography Research Articles

Combination of Electron Beam Surface Structuring and Plasma Electrolytic Oxidation for Advanced Surface Modification of Ti6Al4V Alloy

The objective of this work is to study for the first time the combination of electron beam (EB) surface structuring and plasma electrolytic oxidation (PEO) with the aim of providing a multiscale topography and bioactive surface to the Ti6Al4V alloy for biomedical applications. Ca and P-containing coatings were produced via 45 s PEO treatments over multi-scale EB surface topographies. The coatings morphology and composition were characterized by a means of scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The effect on the previous EB topography was evaluated by means of a 3D optical profilometry and electrochemical response via potentiodynamic polarization tests. In general, the PEO process, morphology, composition and growth rate of the coatings were almost identical, irrespective of the topography treated. Minimal local differences were found in terms of morphology, and the growth rate were related to specific topographical features. Nevertheless, all the PEO-coated substrates presented essentially the same corrosion resistance. Electrochemical tests revealed a localized crevice corrosion susceptibility of all the bare EB topographies, which was successfully prevented after the PEO treatment.

T cell microvilli simulations show operation near packing limit and impact on antigen recognition

T cells are immune cells that continuously scan for foreign-derived antigens on the surfaces of nearly all cells, termed antigen-presenting cells (APCs). They do this by dynamically extending numerous protrusions called microvilli (MVs) that contain T cell receptors toward the APC surface in order to scan for antigens. The number, size, and dynamics of these MVs, and the complex multiscale topography that results, play a yet unknown role in antigen recognition. We develop an anatomically informed model that confines antigen recognition to small areas representing MVs that can dynamically form and dissolve and use the model to study how MV dynamics impact antigen sensitivity and discrimination. We find that MV surveillance reduces antigen sensitivity compared with a completely flat interface, unless MV are stabilized in an antigen-dependent manner, and observe that MVs have only a modest impact on antigen discrimination. The model highlights that MV contacts optimize the competing demands of fast scanning speeds of the APC surface with antigen sensitivity. Our model predicts an interface packing fraction that corresponds closely to those observed experimentally, indicating that T cells operate their MVs near the limits imposed by anatomical and geometric constraints. Finally, we find that observed MV contact lifetimes can be largely influenced by conditions in the T cell/APC interface, with these lifetimes often being longer than the simulation or experimental observation period. This work highlights the role of MVs in antigen recognition.

Laser ablation combined with Nanoimprint Lithography technology as new surface engineering approach to produce novel polymer-based heteronucleants for recalcitrant protein crystallization

Macromolecule crystallography is currently the most powerful technique for determining the three dimensional structure of proteins at atomic resolution. However, despite its enormous level of technical development achieved, it is still limited by the great difficulty of obtaining crystals that, after exposure to X-rays, allow a high-resolution diffraction pattern from which the structure of the target protein can be elucidated. Even today, the crystallization process is a trial-and-error procedure that requires enormous experimental effort and high economic costs. In this research work, Laser Ablation technology in combination with NanoImprint Lithography was applied on Polycarbonate surfaces to produce novel heteronucleating agents containing multiscale surface topographies (in the micro- and submicro-scale) that promote the growth of protein crystals. Two human proteins, known to be recalcitrant to crystallization when using standard protocols, were used in the assays: (i) a construct of the CNNM4 magnesium transporter lacking amino acid residues 545–730 (HsCNNM4545-730) and (ii) cystathionine beta-synthase enzyme lacking residues 516–525 (HsCBSΔ516-525). The surface engineering solution was validated by comparing the number of novel crystallization conditions induced by those surfaces compared against the control and the use of the Naomi’s Nucleant, a commercially available heteronucleant made of mesoporous bioglass. The aspect ratio of the topography emerges as the topographical parameter that most contributes to protein growth on patterned surfaces. Additionally, the presence of a random distribution of micropores as well as a hierarchical (merging micro- and nano - scale features) surface topography also resulted in a more efficient crystallization process, showing an increment of between 38 and 68% in the number of new crystallization conditions, depending on the surface topography and protein selected. A conclusion is made that Laser Ablation with ultrashort pulses and the combination with Nanoimprint Lithography can be efficiently applied on polymeric substrates to produce a novel concept of heteronucleants that would bridge the gap to the development of universal nucleation agents.

Fabrication of succinate-alginate xerogel films for in vitro coupling of osteogenesis and neovascularization

The osseointegration of metallic implants is reliant on a cascade of molecular interactions and the delivery of macromolecules to the implant environment that occurs before substantial bone formation. Early blood vessel formation is a requisite first step in the healing timeline for osteoid formation, where vascular development can be accelerated as a result of controlled hypoxic conditioning. In this study, alginate-derived xerogel films containing varied concentrations of disodium succinate salt which has been shown to induce pseudohypoxia (short-term hypoxic effects while maintaining an oxygenated environment) were developed. Xerogels were characterized for their morphology, succinate release over time and cellular response with osteoblast-mimicking Saos-2 and human umbilical vein endothelial cells (HUVEC). Scanning electron microscopy revealed a multiscale topography that may favour osseointegration and alamarBlue assays indicated no cytotoxic effects during in vitro proliferation of Saos-2 cells. pH measurements of eluted succinate reach 95 % of peak value after 7 h of immersion for all gels containing 10 mM of succinate or less, and 60 % within the first 40 min. In vitro exposure of HUVECs to succinate-conditioned media increased the net concentration of total proteins measured by bicinchoninic acid (BCA) assay and maintains stable vascular endothelial growth factor (VEGF) and extracellular platelet-derived growth factor (PDGF) for vessel formation through comparison of enzyme-linked immunosorbent assays (ELISAs) of the culture media and cell lysate. Tube formation assays also showed a sustained increase in tube diameter across the first 48 h of HUVEC culture when succinate concentrations of 1 and 10 μM in the xerogel. Overall, the succinate-alginate films serve as a prospective organic coating for bone-interfacing implant materials which may induce temporary pseudohypoxic conditions favourable for early angiogenesis and bone regeneration in vivo at succinate concentrations of 1 or 10 μM.

Fibroblast cell responses to physical cues of the triangular prism micropattern and aligned nanofibrous scaffold for promoting wound closure

Cells are sensitive to the microenvironment and multiscale surface topographies were crucial for modulating cell activities and promoting the wound healing process. Herein, we fabricated an engineered microstructural topography surface on the nanofibre scaffold as mimicking a cellular microenvironment. The scaffold consisted of nanofibre matrices arranged in a triangular prism micropattern with either aligned or random nanofibres. The morphological analysis revealed that the scaffolds had a compact layer of nanofibres, and the peak of the triangular prism was present after separation. The in-vitro analysis presents that the human dermal fibroblast cells (HDF) are distributed homogenously and aligned along the structural scaffold. The triangular prism micropattern supported cell movement, resulting in improved cell elongation behavior. In in-vivo analysis, the aligned microstructural scaffold demonstrated a better therapeutic effect and advanced epithelialization on day 7. The length of the epidermis tongue of the aligned and random microstructural scaffold was 1983 ± 132.94 µm and 1638.04 ± 115.85 µm, respectively. By day 14, the aligned microstructural scaffold had enhanced the wound healing process by decreasing the wound area and had achieved a 97% wound closure. This indicated that the aligned microstructural scaffold was able to guide the cell movement required for wound healing application.

Surface Area and Local Curvature: Why Roughness Improves the Bioactivity of Neural Implants.

While roughening the surface of neural implants has been shown to significantly improve their performance, the mechanism for this improvement is not understood, preventing systematic optimization of surfaces. Specifically, prior work has shown that the cellular response to a surface can be significantly enhanced by coating the implant surface with inorganic nanoparticles and neuroadhesion protein L1, and this improvement occurs even when the surface chemistry is identical between the nanoparticle-coated and uncoated electrodes, suggesting the critical importance of surface topography. Here, we use transmission electron microscopy to characterize the topography of bare and nanoparticle-coated implants across 7 orders of magnitude in size, from the device scale to the atomic scale. The results reveal multiscale roughness, which cannot be adequately described using conventional roughness parameters. Indeed, the topography is nearly identical between the two samples at the smallest scales and also at the largest scales but vastly different in the intermediate scales, especially in the range of 5-100 nm. Using a multiscale topography analysis, we show that the coating causes a 76% increase in the available surface area for contact and an order-of-magnitude increase in local surface curvature at characteristic sizes corresponding to specific biological structures. These are correlated with a 75% increase in bound proteins on the surface and a 134% increase in neurite outgrowth. The present investigation presents a framework for analyzing the scale-dependent topography of medical device-relevant surfaces, and suggests the most critical size scales that determine the biological response to implanted materials.

Response of a westerly-trough rainfall episode to multi-scale topographic control in southwestern China

This study aims to quantify the response of a westerly-trough rainfall episode that occurred in summer 2020 to multi-scale topographic control in southwestern China, based on observations and numerical simulations. The multi-scale topography is composed of the Tibetan Plateau, Hengduan Cordillera (HC), and Sichuan Basin (SB). The westerly trough was characterized by southeastward deepening together with an in-phase propagating rainfall episode. By utilizing the results of numerical experiments, how the multi-scale topography impacted this westerly trough rainfall episode is explored. It is found that HC was the pivotal topographic factor affecting the southeastward extension of the trough and related rainfall, while SB accerelated the eastward movement of the westerly trough and changed the tilting direction of the trough line, thus further changing the location and orientation of precipitation. For extreme rainfall with intensity exceeding 10 mm h−1, a roughly threefold rise in the cover ratio (from 1.8% to 7.2%) and fourfold increase in the areal rainfall amount per hour occurred by removing the HC barrier, due to the strongest vorticity and long-distance transport capacity to potential vorticy mass accompanying the southeast-stretching trough. Our results quantitatively reveal a strong response of westerly trough rainfall to multi-scale topographic control in southwestern China, therefore serving as an important reference for future decision making and effective model improvement.摘要中国西南部地形复杂, 降水频发, 地形对降水的影响至关重要. 本文基于观测和数值模拟, 定量揭示了青藏高原, 横断山脉和四川盆地多尺度地形对该地区西风槽降水的影响. 发现横断山脉是影响槽东南伸展, 降水传播的关键地形要素, 而四川盆地可加速西风槽东移, 改变槽线倾斜方向, 进而改变降水的位置和方向. 对于极端降水事件, 移除横断山脉屏障后, 降水覆盖率约增加3倍 (从1.8%增至7.2%), 小时面雨量增强4倍. 这些研究, 可为地形复杂地区降水的未来预报决策和有效模式改进提供参考.

Extracting Background Secondary Craters Based on Fusion of Multiscale and Multifacies Crater Topography Information

Secondary craters (i.e., secondaries) formed by the landing of impact ejecta are an important crater population in planetary geology. Typical secondaries on planetary surfaces occur in chains and clusters, and they have irregular-shaped crater rims and/or floors. Background secondaries are a special type of secondaries that were formed by more-dispersed impact ejecta, and they have similarly circular morphology and dispersed spatial distribution with same-sized primary craters (i.e., primaries) that are formed by asteroids and comets. While individual background secondaries have been recognized at different planetary bodies, there is no reliable method to differentiate populations of background secondaries from those of primaries. In this work, we design a novel method to extract background secondaries based on multi-scale and multi-facies topography characteristics of impact craters. To characterize the complex topography fluctuations across continuous ejecta deposits of secondaries, we design a multi-wavelet method to present their full-wavelength topography variations. Selecting three populations of secondaries formed by the Orientale basin and a population of primaries, we investigate and compare their crater interior slopes and roughness of continuous ejecta deposits, determining the threshold ranges that can differentiate background secondaries from same-sized and coeval primaries. The two sets of threshold values are then fused together to create a Standard T-Score Terrain Indicator, which are combined with our modelled theoretical spatial distribution of secondaries, abundant previously-unrecognized Orientale background secondaries were discovered. Our results demonstrate that production of background secondaries is much more efficient than manifested by the observed spatial density of obvious secondaries in chains and clusters. This study establishes a promising method to detect populations of background secondaries, which can be extended further to smaller primary craters on various airless planetary bodies.

A novel multiscale model for contact behavior analysis of rough surfaces with the statistical approach

This paper presents a contact model to describe the normal and tangential contact behaviors of the rough interface with multiscale topographies. The coupling effect of macroscopic profile deviation and mesoscopic roughness on the contact behaviors is taken into account. First, an improved force expression of single contacting asperity is proposed to characterize the continuous and monotonic feature in the whole deformation process. By adopting the statistical summation, the contact model for the surface with microscopic topography is established. Second, the mesoscopic waviness deformation is characterized with microscopic asperities through multiscale modeling. The macroscopic geometric deviation is defined as a linear shape function. By combining the macro-, meso-- and microscopic topographies, the multiscale normal contact model is established. Then, in terms of tangential model, the Jenkins element is applied to express the slip-stick behavior of asperity. Based on the normal contact model and Coulomb law, the distribution function of the yield slip force is obtained to establish the tangential contact model. Furthermore, the predicted contact responses are compared with experiments and finite element model to validate the effectiveness of the proposed model. Finally, the influences of the multiscale topographies are analyzed, presenting the variation tendencies of contact responses. This work provides a feasible and effective way for studying the contact response of rough interface with multiscale surface topographies.

Variation Characteristics of Summer Water Vapor Budget and Its Relationship with the Precipitation over the Sichuan Basin

Based on the daily precipitation data from the meteorological stations in Sichuan and the monthly average ERA-Interim reanalysis data from 1979 to 2016, the variation characteristics of summer water vapor budget in the Sichuan Basin and its relationship with precipitation are discussed in this study. The results show that, in summer, the water vapor in the Sichuan Basin and its four sub-basins flows in from the southern and western boundaries and flows out through the eastern and northern boundaries, and the basin is obviously a water vapor sink. From 1979 to 2016, the water vapor inflow from the southern and western boundaries significantly decreased, as well as the water vapor outflow through the eastern boundary. The summer precipitation in the Sichuan Basin is significantly positively correlated with the water vapor inflow at the southern boundary and net water vapor budget of the basin in the same period, and it is negatively correlated with the water vapor outflow at the northern boundary. The southern and northern boundaries are the two most important boundaries for the summer precipitation in the Sichuan Basin. Additionally, this study reveals that, under the multi-scale topography on the east side of the Tibet Plateau, the spatio-temporal distribution of precipitation in the Sichuan Basin results from the interactions between the unique topography of the Sichuan Basin and the different modes of water-vapor transport from low latitudes. The atmospheric circulation over the key area of air–sea interaction in the tropical region and its accompanying systems, as well as the anomalies of regional circulations and water vapor transport over the eastern China and Sichuan Basin, are the main reasons for the variation in summer precipitation in the Sichuan Basin.

Plasma-assisted multiscale topographic scaffolds for soft and hard tissue regeneration

The design of transplantable scaffolds for tissue regeneration requires gaining precise control of topographical properties. Here, we propose a methodology to fabricate hierarchical multiscale scaffolds with controlled hydrophilic and hydrophobic properties by employing capillary force lithography in combination with plasma modification. Using our method, we fabricated biodegradable biomaterial (i.e., polycaprolactone (PCL))-based nitrogen gas (N-FN) and oxygen gas plasma-assisted flexible multiscale nanotopographic (O-FMN) patches with natural extracellular matrix-like hierarchical structures along with flexible and controlled hydrophilic properties. In response to multiscale nanotopographic and chemically modified surface cues, the proliferation and osteogenic mineralization of cells were significantly promoted. Furthermore, the O-FMN patch enhanced regeneration of the mineralized fibrocartilage tissue of the tendon–bone interface and the calvarial bone tissue in vivo in rat models. Overall, the PCL-based O-FMN patches could accelerate soft- and hard-tissue regeneration. Thus, our proposed methodology was confirmed as an efficient approach for the design and manipulation of scaffolds having a multiscale topography with controlled hydrophilic property.

An efficient multiscale strategy to predict the evolution of the real contact area between rough surfaces

An efficient and general multiscale strategy to model rough contact and determine the evolution of the real contact area is proposed, based on the splitting of the surface power spectrum. A multiplicative homogenisation scheme is developed to evaluate the contact area fraction effectively, by incorporating statistical information of the contact pressure field in the scale transitions. A strategy for separating the roughness frequencies and for generating the topography at each scale is proposed. The comparison between direct numerical simulation and the proposed multiscale strategy demonstrates the capability to predict the contact area evolution of multiscale rough topographies. The significant reduction of the computational cost endorses the applicability of the proposed multiscale framework to practical circumstances.

Biomimetic Approach for the Elaboration of Highly Hydrophobic Surfaces: Study of the Links between Morphology and Wettability.

This investigation of morphology-wetting links was performed using a biomimetic approach. Three natural leaves’ surfaces were studied: two bamboo varieties and Ginkgo Biloba. Multiscale surface topographies were analyzed by SEM observations, FFT, and Gaussian filtering. A PDMS replicating protocol of natural surfaces was proposed in order to study the purely morphological contribution to wetting. High static contact angles, close to 135, were measured on PDMS replicated surfaces. Compared to flat PDMS, the increase in static contact angle due to purely morphological contribution was around 20. Such an increase in contact angle was obtained despite loss of the nanometric scale during the replication process. Moreover, a significant decrease of the hysteresis contact angle was measured on PDMS replicas. The value of the contact angle hysteresis moved from 40 for flat PDMS to less than 10 for textured replicated surfaces. The wetting behavior of multiscale textured surfaces was then studied in the frame of the Wenzel and Cassie–Baxter models. Whereas the classical laws made it possible to describe the wetting behavior of the ginkgo biloba replications, a hierarchical model was developed to depict the wetting behavior of both bamboo species.

Anti-icing properties of femtosecond laser-induced nano and multiscale topographies

Surfaces covered with micro, nano and multiscale topographies are the subject of significant interest due to their ice-repellency properties in the last decade. Ultrafast laser processing is becoming a widely used clean manufacturing technique to fabricate ordered nano and multiscale topographies on metallic surfaces over relatively large areas. In this work, single-tier nano and two-tier multiscale topographies were fabricated on stainless steel surfaces with one-step femtosecond laser processing to impart anti-icing response. Droplet freezing and frost formation on laser treated surfaces were examined at −10 °C and compared with the lubricant-impregnated and superhydrophobic nanoparticle-coated ones. While the hydrophilic nanoscale topography has accelerated the droplet freezing and performed worse than untreated surfaces, their hydrophobic counterparts increased the time to freezing by nearly two times. Overall, the superhydrophobic two-tier multiscale topography has significantly delayed both the droplet freezing time and frost formation on surfaces. Furthermore, the two-tier multiscale topography has sustained its anti-icing response even after being subjected to 25 abrasion cycles, while the surfaces with only nanoscale topography and nanoparticle-coating lost their functionality in only 10 cycles. Therefore, such robust two-tier multiscale topographies devoid of any coatings can enable and underpin many industrial applications where ice accumulation on surfaces results in performance degradation, e.g. in aerospace, refrigeration, air-conditioning and energy applications.

Comprehensive topography characterization of polycrystalline diamond coatings

The surface topography of diamond coatings strongly affects surface properties such as adhesion, friction, wear, and biocompatibility. However, the understanding of multi-scale topography, and its effect on properties, has been hindered by conventional measurement methods, which capture only a single length scale. Here, four different polycrystalline diamond coatings are characterized using transmission electron microscopy to assess the roughness down to the sub-nanometer scale. Then these measurements are combined, using the power spectral density (PSD), with conventional methods (stylus profilometry and atomic force microscopy) to characterize all scales of topography. The results demonstrate the critical importance of measuring topography across all length scales, especially because their PSDs cross over one another, such that a surface that is rougher at a larger scale may be smoother at a smaller scale and vice versa. Furthermore, these measurements reveal the connection between multi-scale topography and grain size, with characteristic scaling behavior at and slightly below the mean grain size, and self-affine fractal-like roughness at other length scales. At small (subgrain) scales, unpolished surfaces exhibit a common form of residual roughness that is self-affine in nature but difficult to detect with conventional methods. This approach of capturing topography from the atomic- to the macro-scale is termed comprehensive topography characterization, and all of the topography data from these surfaces has been made available for further analysis by experimentalists and theoreticians. Scientifically, this investigation has identified four characteristic regions of topography scaling in polycrystalline diamond materials.

A self-adaptive two-parameter method for characterizing roughness of multi-scale subglacial topography

AbstractDuring the last few decades, bed-elevation profiles from radar sounders have been used to quantify bed roughness. Various methods have been employed, such as the ‘two-parameter’ technique that considers vertical and slope irregularities in topography, but they struggle to incorporate roughness at multiple spatial scales leading to a breakdown in their depiction of bed roughness where the relief is most complex. In this article, we describe a new algorithm, analogous to wavelet transformations, to quantify the bed roughness at multiple scales. The ‘Self-Adaptive Two-Parameter’ system calculates the roughness of a bed profile using a frequency-domain method, allowing the extraction of three characteristic factors: (1) slope, (2) skewness and (3) coefficient of variation. The multi-scale roughness is derived by weighted-summing of these frequency-related factors. We use idealized bed elevations to initially validate the algorithm, and then actual bed-elevation data are used to compare the new roughness index with other methods. We show the new technique is an effective tool for quantifying bed roughness from radar data, paving the way for improved continental-wide depictions of bed roughness and incorporation of this information into ice flow models.

Multiscale investigation of olivine (0 1 0) face dissolution from a surface control perspective

The surface reaction of olivine materials is critical for their extensive applications in the global cycle of elements, future CO2 sequestration and fuel production. Here, we investigate a pristine (010) surface dissolved in flow-through cells with acidic solutions at surface control regime. The multiscale surface topography changes during dissolution are firstly measured by a combination of ex-situ vertical scanning interferometry and in-situ atomic force microscopy (AFM). The deduced dissolution rate maps and rate spectra from surface topography vary temporally and spatially, wherein the average rate is higher at initial stages and reaches to a constant value, about 6.6 ± 1 × 10−8 mol m−2 s−1, after 100 h reaction accompanied with the formation of crystallographically controlled pits. Furthermore, in-situ AFM shows that the average height of steps on the pit walls is equal to 1/2b and Raman spectra prevails that the (010) surface is favorably dissolved layer by layer at Mg2 glide plane with SiO44− distortion. This work reveals that at far from equilibrium conditions with constant Gibbs free energy, surface reactivity is better defined by rate ranges rather than a single mean rate. This superior method is powerful to quantify surface reaction variabilities in multiscale range and understand the geochemical cycles.

Analysis of complex modal instability of a minimal friction self-excited vibration system from multiscale fractal surface topography

In order to reveal the stability and nonlinear characteristics of the friction self-excited vibration system from the perspective of microscopic multiscale fractal surface topography, a minimal two-degree-of-freedom mathematical model of the disc brake system is established in this paper. Considering the fractal characteristics of rough surface topography, the fractal contact stiffness of contact surfaces is introduced into the system model from the perspective of microscopic contact, and the effects of two important surface fractal parameters, fractal dimension D and fractal roughness G, on the stability and nonlinearity of the system are analyzed. Further finite element analysis and brake noise test improve the analysis of system stability and noise intensity by different surface topographies. The results show that the system stability can be improved with the increase of fractal dimension within a certain range, and the system will be in a stick state with the further increase of fractal dimension. With the increase of fractal roughness, the unstable modal coupling region of the system is increasing, which can reduce the stability of the system. The unstable region of the system is dependent on fractal dimension and fractal roughness, and has different sensitivity to them.

Controlling the Multiscale Topography of Anodized Aluminum Oxide Nanowire Structures for Surface-Enhanced Raman Scattering and Perfect Absorbers.

In this study, a plasmonically active substrate is developed with the aim of controlling the perfect absorption and manipulating its optical properties for application in SERS (in NIR regime) and colorimetry. Based on modified fabrication method of anodized aluminum oxide (AAO), the cost-effective self-aggregation technique is presented to fabricate unique topography of bone-fire-like funnel-shaped collapsed and vertically aligned nanowire structures. The length of the nanowire and the modification of surface topography induced by capillary force inside the nanowire are set to structural parameters, and the effect of their changes is closely studied. After deposition of 40 nm gold (Au) film on numerous AAO nanowire structures with different wire lengths and unique topography, the localized surface plasmon resonance excitation is generated, and also its application on reflection and SERS spectra have been shown quantitatively. The length of the wire and surface topography modification are identified as suitable parameters to tune the reflection/absorption (from <40 to >90%) as well as colorimetric effect. Finally, an optimized wire length of Au-coated AAO substrate in SERS sensing application with 3.92 × 105 order of enhancement of rhodamine 6G (R6G) Raman signal is demonstrated.

Femtosecond laser-induced sub-micron and multi-scale topographies for durable lubricant impregnated surfaces for food packaging applications

Adhesion of viscous liquids on packaging surfaces could lead to wastage, an increase of recycling costs, and even customers' dissatisfaction in applications related to food, cosmetics and agrochemical industries. Lubricant-impregnated surfaces (LIS) gained much attention recently over other surface functionalisation technologies due to their non-sticking response to highly viscous liquids. This work reports an investigation into anti-adhesive properties of LIS, with an emphasis on their durability. It provides an insight into the rationale design of LIS topographies in order to maximise their lubricant retention in potential food packaging applications. Femtosecond laser processing and hot embossing were employed to produce two types of topographies for LIS on stainless steel, polypropylene and polystyrene surfaces. The first type was single-scale sub-micron laser induced periodic surface structures (LIPSS), while the second one was multi-scale (MS) structures with both micron and sub-micron features. Droplet shedding characteristics of such LIPSS-LIS and MS-LIS substrates with water, milk and honey were examined under vibration and shear. The critical sliding angles at which liquid droplets attained motion on LIS were observed to be less than 32° for all investigated liquids. However, the LIPSS-LIS substrates retained their functionality even after subjecting them to severe vibration, while the MS-LIS substrates partially lost their anti-adhesive characteristics. At the same time, the MS-LIS substrates exhibited premature pinning of droplets as compared to LIPSS-LIS substrates, under shear forces. Both vibration- and shear-induced loss of lubricant impacted the MS-LIS functionality.