Potential Monitoring Research Articles

Stimulus-responsive magnetic enzyme-encapsulated coordination polymer for the potential field monitoring of alkaline phosphatase using personal glucometer

Alkaline phosphatase (ALP) is an enzyme that can hydrolyze phosphate groups, and its activity is related to algal bloom outbreaks in eutrophic waters. A magnetic ALP-responsive material named Fe3O4@INV/Zn-AMP was synthesized at room temperature by using zinc ions and adenosine monophosphate (AMP) as building blocks integrated invertase (INV). When ALP is present, it hydrolyzes the phosphate group of AMP, causing the disassembly of the Fe3O4@INV/Zn-AMP material and the release of signaling molecule (INV). After magnet separation, the released INV can digest sucrose into glucose measured by a personal glucometer (PGM), providing a signal that indicates the activity of ALP. This method allows for selective, sensitive, and easy detection of ALP in field settings without the need for complicated labeling operations. Benefit from the selective stimulus–response and the enzyme catalyzed amplification, a simple and portable method for the monitoring of ALP using PGM was established, with linear range of 0.005–0.2 U/mL and a detection limit of 0.001 U/mL, which showed the potential of field monitoring for alkaline phosphatase in the future.

Intraoperative neuromonitoring of visual evoked potentials in a pregnant patient with meningioma: a case report

BackgroundMeningioma in the parasellar region may lead to visual impairment, so intraoperative neurological monitoring is essential for enucleation surgery. However, intraoperative neurological monitoring in pregnant women is challenging, as the anesthesia management must consider the effects and risks to the fetus. Remimazolam is a newly introduced intravenous anesthetic that has little effect on blood pressure. However, the effects of remimazolam on intraoperative neuromonitoring are little known. We treated a pregnant patient with parasellar meningioma who developed visual impairment, using remimazolam for anesthesia and intraoperative neurophysiological monitoring of the visual evoked potential.Case presentationA 34-year-old woman who was 20 weeks pregnant presented with visual acuity disturbances. Neuroimaging demonstrated a parasellar meningioma, and rapid tumor growth and worsening of symptoms subsequently occurred. Craniotomy for tumor removal was performed under anesthesia with remimazolam, which allowed monitoring of the visual evoked potentials. Her visual acuity was restored postoperatively, and no adverse events occurred in the fetus.ConclusionOur experience with intraoperative neuromonitoring of a pregnant woman in the third trimester showed that anesthesia with remimazolam allows safe brain surgery combined with intraoperative visual evoked potential monitoring. Further research is needed to determine the effects of remimazolam on the fetus, as well as the safe dosage and duration of exposure.

Scalp surface Laplacian potential monitoring system based on novel hydrogel active tri-polar concentric ring electrodes

Electroencephalography is valued in brain research for its high temporal resolution and user-friendly nature. However, limitations in spatial resolution and susceptibility to biological artifacts restrict its broader application. The surface Laplacian potential obtained through tri-polar concentric ring electrodes offers a solution by mitigating biological artifact interference and enhancing spatial resolution while preserving essential information. In this study, an active tri-polar concentric ring electrode based on conductive hydrogel and a 3-lead surface Laplacian potential monitoring system designed for use with the electrode were proposed. The part of the electrode that came into contact with the human body was the hydrogel with high electrical conductivity. The contact surface curvature was designed based on the real forehead curvature of 22 subjects, resulting in a normalized electrode-skin contact impedance of 30.95±16.76KΩ*cm2 at 10 Hz, which was lower than commercial disposable wet electrodes and remained stable over a 10-hour period of long-term wear. And the electrode still exhibited excellent performance after being left for 20 days. Additionally, the internal noise of the system was about 1.9μV, which was comparable to that of the FDA-approved equipment. The signal analysis results demonstrated that the surface Laplacian potential collected by the proposed electrode and system had better signal-to-noise ratio and spatial resolution, and had a good suppression effect on biological artifacts.

Improvement of Error-Related Potential Monitoring in Brain–Computer Interface Based on Optimal Feature Dimensionality Selection

Improvement of Error-Related Potential Monitoring in Brain–Computer Interface Based on Optimal Feature Dimensionality Selection

Activation mechanism of persulfate by Fe3C-based materials for efficient benzo-a-pyrene abatement in wastewater: The reversed direct electron transfer from persulfate to contaminants

Herein, an unusual reserved direct electron transfer (r-DET) process from persulfate (PS) to contaminants was observed on the surface of Fe3C with N-doped C shell supported on carbon nanotubes (Fe3C@CN-CNTs). The Fe3C-based materials were synthesized via a facile sol–gel method employing melamine, glucose, FeCl3, and commercial multi-walled CNTs. The activation mechanisms were fully explored with a comparison of Fe3C@CN-CNTs and other Fe3C-based materials for the effective abatement of benzo-a-pyrene (BaP), a typical persistent organic pollutant in industrial wastewater. Electrochemical measurements, zeta potential monitoring, and Mössbauer spectroscopy indicated that the lack of CN shell result the relatively large particle size of Fe3C on Fe3C-CNTs, leading to more negatively charged surface and abundant OH during PS activation. Nevertheless, the superparamagnetic fraction with a fine particle size on the surface of Fe3C@CN and Fe3C@CN-CNTs potentially leads to a more positively charged surface, enhancing the zeta potential of surface adsorbed BaP and benefiting the r-DET. Through r-DET, oxidized PS and reduced BaP formed as intermediates, accelerating the overall degradation of BaP in Fe3C@CN-CNTs/PS. The degradation pathways, cytotoxicity, and operation conditions of BaP degradation by Fe3C@CN-CNTs/PS were thoroughly investigated. Additionally, Fe3C@CN-CNTs were found to promote the nucleophilic reaction between Cl− and PS, producing free chlorine and enhancing BaP degradation efficiency in chloride-containing wastewater. Overall, this study presents a new insight for the PS-based r-DET process and offers a promising approach for treating organically-contaminated wastewater.

High-affinity antibodies specific to the core region of the tau protein exhibit diagnostic and therapeutic potential for Alzheimer’s disease

BackgroundRecent advances in blood-based biomarker discovery are paving the way for simpler, more accessible diagnostic tools that can detect early signs of Alzheimer’s disease (AD). Recent successes in the development of amyloid-targeting immunotherapy approaches mark an important advancement in providing new options for the treatment of AD. We have developed a set of high-affinity monoclonal antibodies (mAbs) to tau protein that have the potential as tools for diagnosis and treatment of AD.MethodsSheep were immunised with either full-length tau (1-441) or truncated paired helical filament (PHF)-core tau (297–391). A stringent bio-panning and epitope selection strategy, with a particular focus directed to epitopes within the disease-relevant PHF-core tau, was used to identify single-chain antibodies (scAbs). These scAbs were ranked by affinity for each epitope class, with leads converted to high-affinity mAbs. These antibodies and their potential utility were assessed by their performance in tau immunoassays, as well as their ability to prevent tau aggregation and propagation. Further characterisation of these antibodies was performed by immunohistochemical staining of brain sections and immuno-gold electronmicroscopy of isolated PHFs.ResultsOur work resulted in a set of high-affinity antibodies reacting with multiple epitopes spanning the entire tau protein molecule. The tau antibodies directed against the core tau unit of the PHF inhibited pathological aggregation and seeding using several biochemical and cell assay systems. Through staining of brain sections and PHFs, the panel of antibodies revealed which tau epitopes were available, truncated, or occluded. In addition, highly sensitive immunoassays were developed with the ability to distinguish between and quantify various tau fragments.ConclusionThis article introduces an alternative immunodiagnostic approach based on the concept of a “tauosome” – the diverse set of tau fragments present within biological fluids. The development of an antibody panel that can distinguish a range of different tau fragments provides the basis for a novel approach to potential diagnosis and monitoring of disease progression. Our results further support the notion that tau immunotherapy targeting the PHF-core needs to combine appropriate selection of both the target epitope and antibody affinity to optimise therapeutic potential.

Corrosion of Copper, Cupronickel, Nickel Aluminium Bronze and Super-Duplex Stainless Steel Rotating Cylinder Electrodes in Seawater under Turbulent Flow Conditions

The corrosion of uncoated metals in a marine environment restricts the choice of suitable engineering metals and alloys. Anodic dissolution of metal and cathodic reduction of oxygen are important processes and formation of a surface oxide film can affect both electrode reactions. Charge transfer and mass transfer data can provide information on corrosion rate and mechanism. Several metals and alloys having a history of use in marine engineering are considered, including copper, two copper alloys (cupronickel and nickel aluminium bronze) plus a more recent addition to strong, corrosion resistant alloys (a super duplex stainless steel). The rotating cylinder electrode offers the benefits of a controlled, turbulent flow of electrolyte, facilitating controlled mass transfer in a compact cell geometry which is well-suited to bench-top operation. These features are illustrated using a variety of electrochemical techniques, including open-circuit potential vs time monitoring, linear sweep voltammetry, rotation speed step- or potential step current transients and linear polarisation resistance measurements. Seawater taken from Langstone Harbour, Hampshire, UK, was filtered, air-saturated, pH 8.0 and maintained at 25 °C. Dimensionless group correlations and graphical plots (using an analogous approach to the Koutecky-Levich equation for an RDE) enabled contributions of charge transfer-, mixed- and mass transfer- controlled data to be appreciated, allowing the Tafel slopes of electrode reactions, the diffusion coefficient of dissolved oxygen, the mass transfer coefficient for oxygen reduction and the mean corrosion rate to be estimated.

A machine learning approach to map the potential agroecological complexity in an indigenous community of Colombia

Agroecological systems are potential solutions to the environmental challenges of intensive agriculture. Indigenous communities, such as the Kamëntšá Biyá and Kamëntšá Inga from the Sibundoy Valley (SV) in Colombia, have their own ancient agroecological systems called chagras. However, they are threatened by population growth and expansion of intensive agriculture. Establishing new chagras or enhancing existing ones faces impediments such as the necessity for continuous monitoring and mapping of agroecological potential. However, this method is often costly and time consuming. To address this limitation, we created a digital map of the Biodiversity Management Coefficient (BMC) (as a proxy of agroecological potential) using Machine Learning. We utilized 15 environmental predictors and in-situ BMC data from 800 chagras to train an XGBoost model capable of predicting a multiclass BMC structure with 70% accuracy. This model was deployed across the study area to map the extent and spatial distribution of BMC classes, providing detailed information on potential areas for new agroecological chagras as well as areas unsuitable for this purpose. This map captured footprints of past and present disturbance events in the SV, revealing its usefulness for agroecological planning. We highlight the most significant predictors and their optimal values that trigger higher BMC status.

A review of potential fault detection methods for traditional collector stations and sensor fault diagnosis in wind farm doubly-fed generator converters

Collector stations sometimes face situations where existing relay protection systems may fail, making potential fault monitoring technology increasingly important. This technology plays a critical role in ensuring the stability of power supply and enhancing economic benefits. Simultaneously, in response to the nation's "dual carbon goals," the construction and operation of large-scale renewable energy power stations are booming. Therefore, potential fault monitoring technology for new power stations should not be overlooked. For traditional power stations, the application and development trends of infrared thermal imaging technology, vibration analysis and acoustic detection technology, chemical analysis, and oil quality detection technology will be compared and discussed. Regarding new power stations, methods for diagnosing sensor faults in doubly-fed generator converters in wind power will be analyzed and discussed.

RFID-Based Respiration Monitoring Under Daily Body Motion Disturbances

Traditional respiratory monitoring methods typically use wearable sensors. In recent years, Radio Frequency Identification (RFID) tags have emerged as potential respiratory monitoring devices. However, existing research only detects respiration under static or simple body movements such as forward and backward motions, which limits the availability of RFID. In this article, an RFID-based respiratory monitoring system under body motion disturbances is proposed. In order to eliminate the effect of body movement on the signal, reference tags are placed on the user’s shoulders. The phase between different tags is calibrated and aligned to remove the discreteness. Then, a sliding window peak-seeking algorithm is designed to calculate respiratory rate over time. The system measures respiratory rate during daily exercise and maintains an accuracy of no less than 86.12%, even when influenced by lying down and turning. Additionally, the system can analyze estimated respiratory rates and intervals to provide alert information.

Multicolor Mechanoluminescence From Lu3Al2Ga3O12: Tb, Eu for Stress and Temperature Visual Sensing

AbstractMechanoluminescence (ML) refers to the luminescence phenomenon that occurs when a material is under external mechanical stimuli. However, relying solely on stress information to obtain ML signals is prone to test errors in complex test conditions. In this work, a Tb3+/Eu3+‐doped Lu3Al2Ga3O12 multicolor ML material is reported, in which the ML color can be adjusted from white to red (CIE color coordinates: from (x = 0.313, y = 0.3293) to (x = 0.6183, y = 0.379)) by changing the Eu3+ concentration. In addition, LAGO: 0.25% Tb3+ and LAGO: 1.5% Eu3+ are physically mixed at different mass ratios, and the varied stimuli‐responsed emission characteristics of Tb3+ and Eu3+ ions are used to develop a stress and temperature dual sensing device. Stress and temperature information can be further reflected simultaneously through the blue/red emission ratio IR (ITb/IEu). As the temperature increases, the color changes from white to red (CIE color coordinates: from (x = 0.3259, y = 0.306) to (x = 0.4707, y = 0.3625)), and the relative temperature sensitivity (Sr) is as high as 1.209% K−1 at 298 K. This sensing device provides a new idea for potential structural safety monitoring, multi‐modal anti‐counterfeiting technology, etc.

Towards Sustainable Antibiotic Use in Aquaculture and Antimicrobial Resistance: Participatory Experts' Overview and Recommendations.

Notably, 56 worldwide experts gathered for the Antimicrobial Assessment on Global Aquaculture Production (AGAP) series of workshops to (1) evaluate the current state of knowledge on antimicrobial use and identify existing gaps; (2) formulate strategies to identify ecologically relevant impact indicators and establish thresholds for assessment; (3) identify pivotal socioeconomic factors and effective governance mechanisms essential for implementing monitoring practices in aquaculture and extending them across sectors and countries for aquaculture sustainability; (4) develop pathways to enhance our comprehension between antibiotic use in aquaculture and antimicrobial resistance; and (5) explore potential antibiotic monitoring tools that can be universally adapted and implemented across region and sectors. The main outcomes were a roadmap for establishing investigation priorities on the relevant topics regarding antibiotic use in aquaculture, socioeconomic drivers for using antibiotics and behaviors that need more robust and transparent regulatory frameworks to guide farmers, training on antimicrobial use, and access to veterinarians and extension services agents for education. Overall, the workshop evidenced the power of collaboration in addressing complex global challenges to achieve sustainable aquaculture. Despite diligent efforts, some constraints may have inadvertently narrowed the possibility of having more experts and left some pertinent topics unaddressed, but they are needed in the discussion.

Verification and Evaluation of Male-Produced Pheromone Components from the Citrus Long-Horned Beetle, Anoplophora chinensis (Forster) (Insecta: Coleoptera: Cerambycidae).

The citrus long-horned beetle, Anoplophora chinensis (Coleoptera: Cerambycidae), is a highly polyphagous species native to eastern and southeastern Asia. Detection of these beetles is dependent on visual surveys, which are inefficient and labor-intensive. The identification and development of pheromone-based lures would help improve survey efforts for A. chinensis should it become established within the US. We identified three antennally active male-produced chemical components (nonanal, 4-(n-heptyloxy)butan-1-ol, and 4-(n-heptyloxy)butanal), which were then tested for behavioral activity at our USDA Quarantine laboratory. When tested together in Y-tube behavioral assays, a three-component blend of the identified compounds was shown to be attractive to adult female A. chinensis. Pheromone production and behavior of A. chinensis and other closely related Anoplophora cerambycid species are discussed in relation to mate finding and potential monitoring.

Mitochondrial STED Imaging and Membrane Potential Monitoring with a Cationic Molecular Probe.

Mitochondria are essential organelles that not only undergo dynamic morphological changes but also exhibit functional activities such as mitochondrial membrane potential (MMP). While super-resolution techniques such as stimulated emission depletion (STED) nanoscopy can visualize the ultrastructure of mitochondria and the MMP probe can monitor mitochondria function, few dyes meet both demands. Here, a small molecule (MitoPDI-90) based on perylene diimide with cationic groups is reported and used for mitochondrial STED imaging and MMP indication. Characterized by excellent photostability, biocompatibility, and high quantum yield, MitoPDI-90 exhibits STED imaging compatibility, facilitating visualization of mitochondrial cristae and time-lapse imaging of highly dynamic mitochondria in living cells. Besides, MitoPDI-90 targets the mitochondria through electrical potential, also enabling live-cell MMP monitoring. MitoPDI-90 allows for super-resolution visualization and time-lapse imaging of mitochondria, and more importantly, indication of changes in MMP, providing insight into the functional activity of live-cell mitochondria.

Transcranial Corticospinal Motor-Evoked Potentials in Cases of Ventral and Ventrolateral Intradural Extramedullary Cervical Spinal Cord Tumors.

Background and Objectives: We studied the clinical significance of an amplitude decrement and disappearance alarm criteria in transcranial motor-evoked potential (MEP) monitoring during surgeries on extramedullary tumors at the cervical spine with reference to postoperative morbidity. Material and Methods: We diagnosed and surgically treated fourteen patients with intradural extramedullary ventral or ventrolateral lesions to the cervical spinal cord in the Clinic of Neurosurgery at the University Hospital St Ivan Rilski from January 2018 to July 2022. Eight cases were diagnosed with schwannoma, and the remaining six had meningiomas. The follow-up period for neurological assessment was six months. Results: A decrease in the intraoperative transcranial MEPs of 50% or more compared to baseline in two cases (14.3%) resulted in an immediate postoperative motor deficit. One patient demonstrated full neurological recovery within six months, while the other exhibited only partial improvement. In six cases (42.9%) with preoperative motor deficits, tumor resection and decompression of the cervical spinal cord led directly to an increment of the transcranial MEPs by more than 20%. Postoperatively and at the 6-month follow-up, these patients showed recovery from the preoperative deficits. In the remaining cases, MEPs were stable during surgery with no clinical deterioration of the motor function. Conclusions: The decremented MEP criteria corresponded to postoperative motor deficit, whereas the improvement of the same parameters after decompression implied future recovery of preoperative motor deficits. The combination of different MEP criteria is likely to be helpful when tailored to a specific case of ventral or ventrolateral extramedullary lesions in the cervical spine.

Monitoring of myocardial injury by serial measurements of QRS area and T area: The MaastrICCht cohort.

Manually derived electrocardiographic (ECG) parameters were not associated with mortality in mechanically ventilated COVID-19 patients in earlier studies, while increased high-sensitivity cardiac troponin-T (hs-cTnT) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) were. To provide evidence for vectorcardiography (VCG) measures as potential cardiac monitoring tool, we investigated VCG trajectories during critical illness. All mechanically ventilated COVID-19 patients were included in the Maastricht Intensive Care Covid Cohort between March 2020 and October 2021. Serum hs-cTnT and NT-proBNP concentrations were measured daily. Conversion of daily 12-lead ECGs to VCGs by a MATLAB-based script provided QRS area, T area, maximal QRS amplitude, and QRS duration. Linear mixed-effect models investigated trajectories in serum and VCG markers over time between non-survivors and survivors, adjusted for confounders. In 322 patients, 5461 hs-cTnT, 5435 NT-proBNP concentrations and 3280 ECGs and VCGs were analyzed. Non-survivors had higher hs-cTnT concentrations at intubation and both hs-cTnT and NT-proBNP significantly increased compared with survivors. In non-survivors, the following VCG parameters decreased more when compared to survivors: QRS area (-0.27 (95% CI) (-0.37 to -0.16, p < .01) μVs per day), T area (-0.39 (-0.62 to -0.16, p < .01) μVs per day), and maximal QRS amplitude (-0.01 (-0.01 to -0.01, p < .01) mV per day). QRS duration did not differ. VCG-derived QRS area and T area decreased in non-survivors compared with survivors, suggesting that an increase in myocardial damage and tissue loss play a role in the course of critical illness and may drive mortality. These VCG markers may be used to monitor critically ill patients.

Efficient butanol production from sweet sorghum stem juice by a co-culture system at an optimum temperature: Insights from oxidation-reduction potential monitoring and pilot scale validation

A two-stage co-culture approach was employed in an acetone-butanol-ethanol (ABE) fermentation. An obligate aerobic bacterium, Arthrobacter sp., was first grown for 6 h at 30 °C to create anaerobic conditions. Subsequently, Clostridium beijerinckii TISTR 1461 was inoculated and a fermentation was performed at 37 °C. To identify an intermediate temperature suitable for both microorganisms, their growth was examined at 30, 34, and 37 °C. C.beijerinckii exhibited the highest specific growth rate at 37 °C, while Arthrobacter sp. displayed similar growth rates at all tested temperatures. Butanol production from a synthetic medium (P2 medium) by C. beijerinckii at different temperatures using oxygen-free nitrogen (OFN) gas flushing as a control treatment revealed that fermentation at 37 °C gave the highest butanol concentration (PB, 9.98 g/L). Consequently, 37 °C was chosen for butanol production from sweet sorghum stem juice (SSJ) by co-culture of these two microorganisms in 1-L screw–capped bottles. Compared to the control treatment, higher PB (11.38 g/L), yield (YB/S, 0.37 g/g) and productivity (QB, 0.24 g/L·h) were achieved using the co-culture system. These results were further confirmed by monitoring the oxidation–reduction potential (ORP) during ABE fermentation in a 2-L stirred-tank bioreactor (STR). Moreover, when the co-culture fermentation at 37 °C was scaled up in a 30-L STR, the PB, YB/S and QB values were comparable to those obtained in the 2-L STR. Therefore, co-culture fermentation of Arthrobacter sp. and C.beijerinckii TISTR 1461 at 37 °C represents a promising method for large-scale butanol production.

Systemic toxicity of CAR-T therapy and potential monitoring indicators for toxicity prevention.

Malignant tumors of the hematologic system have a high degree of malignancy and high mortality rates. Chimeric antigen receptor T cell (CAR-T) therapy has become an important option for patients with relapsed/refractory tumors, showing astonishing therapeutic effects and thus, it has brought new hope to the treatment of malignant tumors of the hematologic system. Despite the significant therapeutic effects of CAR-T, its toxic reactions, such as Cytokine Release Syndrome (CRS) and Immune Effector Cell-Associated Neurotoxicity Syndrome (ICANS), cannot be ignored since they can cause damage to multiple systems, including the cardiovascular system. We summarize biomarkers related to prediction, diagnosis, therapeutic efficacy, and prognosis, further exploring potential monitoring indicators for toxicity prevention. This review aims to summarize the effects of CAR-T therapy on the cardiovascular, hematologic, and nervous systems, as well as potential biomarkers, and to explore potential monitoring indicators for preventing toxicity, thereby providing references for clinical regulation and assessment of therapeutic effects.

Predictive nomogram for hearing deficits after microvascular decompression treatment.

We explored the impact of brainstem auditory evoked potentials monitoring, as well as anatomical characteristics, in relation to their influence on hearing deficits. A total of 851 patients diagnosed with idiopathic hemifacial spasm underwent microvascular decompression treatment were recruited in our study. A nomogram was developed based on the regression analysis. Nomogram performance was evaluated through receiver operating characteristic (ROC), decision curve analyses and calibration curve. The rate of positive wave V change was also higher in the hearing deficit group (71.8% vs no hearing deficit group, p < 0.001). Furthermore, greater retraction depth (0.78 ± 0.25cm vs 0.55 ± 0.12cm, p < 0.001), duration (74.43 ± 15.74min vs 55.71 ± 7.01min, p < 0.001) and retraction distance (4.38 ± 0.38cm vs 4.17 ± 0.24cm, p = 0.001) were evident in the hearing deficit patients. Multivariate logistic regression showed that positive wave V change (OR 5.43), greater retraction depth (OR 55.57) and longer retraction duration (OR 1.14) emerged as significant independent predictors of postoperative hearing deficit. The external validation cohort exhibited a favorable discrimination with an AUC of 0.88. The calibration curves further confirmed the reliability of the predicted outcome in relation to the observed outcome in the external validation cohort (p = 0.89). The decision curves demonstrated that the nomogram outperformed the All or None scheme when the threshold probability ranged from > 2% to < 60% in the external validation cohort. We constructed a nomogram, including wave V, retraction depth, and retraction duration, which can effectively predict the occurrence of hearing deficits and has good clinical applicability.

Enhanced pyrophosphate detection: Utilizing oPD-derived carbon dots and Fe3+ interactions in a paper strip biosensor

The development of portable, cost-effective, and straightforward DNA biosensors holds immense importance in various fields, including healthcare, environmental monitoring, and food safety. This study contributes to the objective by introducing an innovative approach for synthesizing carbon dots (Cdots) with high quantum yield (QY) and remarkable selectivity for Fe3+ ions. Utilizing o-phenylenediamine as a precursor, the study achieved a straightforward and environmentally friendly synthesis method, enabling the efficient detachment of metal ions from the Cdot surface upon introducing pyrophosphate (PPi). The presence of surface hydroxyl and amino groups facilitated specific Fe3+ recognition. Employing D-optimal response surface methodology, the study optimized Cdot synthesis parameters, identifying temperature and heating time as critical factors influencing QY. Statistical analysis confirmed the model's reliability, predicting maximum QY of 48.8 % with minimal deviation from experimental results. Characterization studies revealed the amorphous nature of Cdots through HR-TEM, XRD, and FTIR analysis. Furthermore, the proposed LAMP/PPi biosensing technique demonstrated higher sensitivity, specificity, and repeatability, with negligible interference from common anions and efficacy across varying pH levels. The limit of detection (LOD) of 0.079 (±0.01) μM and the detection range of 0.1 μM–2 mM underscore the biosensor's practical utility. This study highlights a promising direction for developing paper-based LAMP/PPi biosensors with potential diagnostics and environmental monitoring applications. Significantly, the biosensing technique is applicable to any DNA amplification method generating pyrophosphate (PPi) as a by-product.