Decrease In Molecular Weight Research Articles

In vitro simulated digestion and fermentation characteristics of polyphenol-polysaccharide complex from Hizikia fusiforme and its effects on the human gut microbiota.

This study investigated the effects of the polyphenol-polysaccharide complex (HPC) and its purified components (PC1 and PC4), obtained from Hizikia fusiforme, on the human gut microbiota during in vitro simulated digestion and fecal fermentation. Results showed a gradual increase in reducing sugar content for HPC, PC1, and PC4 during simulated digestion, accompanied by a slight decrease in molecular weight, indicating that these complexes were not completely digested during oral-gastrointestinal digestion. However, following fermentation, the molecular weights of HPC, PC1, and PC4 decreased significantly, and the molar ratios of monosaccharide compositions changed considerably compared with prefermentation values. Thus, these complexes were degraded and used by the intestinal microbiota to produce short-chain fatty acids, which decreased the pH. In addition, after fecal fermentation, beneficial bacteria such as Bacteroides, Parabacteroides, and Bifidobacterium became more abundant, whereas the amount of harmful bacteria such as Fusobacterium and Escherichia/Shigella decreased, revealing the regulation by the complex on the intestinal microbiota. In conclusion, the polyphenol-polysaccharide complex improves the composition and abundance of the human gastrointestinal microbiota, thereby supporting gut health.

Evaluation of physicochemical property changes in 3D-printed biodegradable medical devices under simulated oral physiological conditions

Biodegradable medical devices undergo degradation following implantation, potentially leading to clinical failure. Consequently, it is necessary to assess the change in their properties post-implantation. However, a standardized method for the precise evaluation of the changes in their physicochemical properties is currently lacking. In this study, we aimed to establish precisely simulated oral physiological conditions (SOPCs) and investigate the physicochemical property changes to predict the performance alterations of biodegradable dental barrier membranes (BDBMs) following human implantation. We investigated changes in physicochemical properties of BDBM after exposure to SOPC for 24 weeks. When BDBM was exposed to SOPC for 24 weeks, there was a significant decrease in mass (-1.37%), molecular weight (-19.54%) and tensile load (-72.84%). Among the physicochemical properties, molecular weight decreased similarly after 24 weeks of implantation in rats (-15.78%) and after 24 weeks of exposure to SOPC (-19.54%). Changes in the physicochemical properties of BDBM in simulatedin vitrooral conditions and in thein vivoenvironment were similar. Overall, the evaluation of physicochemical property changes after exposing BDBM to the proposed SOPC demonstrates novelty in its ability to accurately predict performance changes post-implantation. This approach may provide significant insights not only for the development of BDBM but also for various types of biodegradable medical devices.

Computer modeling of the synthesis of styrene–butadiene rubber: Influence of the feed of a chain transfer agent on the characteristics of the product

Objectives. To develop mathematical approaches and algorithms for analyzing the influence of various methods for feeding a chain transfer agent to a cascade of reactors, taking into account the choice of feed points on the characteristics of the final product of copolymerization using computer modeling.Methods. The processes of synthesis of copolymers were mathematically modeled using a statistical approach (Monte Carlo method). The developed algorithm is based on calculating the probabilities of elementary reactions in the process under study. In the case of continuous production of the copolymer in a cascade of reactors, it must be taken into account that the residence time of each particle of the reaction mixture in the reactor is subject to a probability distribution. The algorithm models the formation of copolymer macromolecules at the particle level, permitting the average molecular characteristics of the copolymer to be calculated and its microstructure to be studied based on modeling results.Results. The dependencies of the intrinsic viscosity on the reactor number and conversion were constructed by means of mathematical modeling. The calculation results showed satisfactory agreement with the experimental data obtained in production. The dependencies of the molecular weight distribution of the copolymer, the weight-average molecular weight, and the microheterogeneity index on the reactor number were constructed for various methods of feed of the chain transfer agent, i.e., to two and/or three points of the reactor cascade. The modeling and calculation results confirmed the influence of the method of adding the chain transfer agent to the cascade reactors on the molecular characteristics of the copolymer.Conclusions. The analysis of the structure of the molecular units of the styrene–butadiene copolymer showed a decrease in the weightaverage molecular weight of the final product and an increase in its stiffness in the case of the three-point feed of the chain transfer agent.

Chemical modification of PLA for the design of 3D printed nanocomposite scaffolds with enhanced degradability for bone tissue engineering

AbstractIn this study, 3D printing technology is used to develop nanocomposite scaffolds based on polylactic acid (PLA) and hydroxyapatite (HA). PLA was functionalized with itaconic anhydride (PLAf) via radical grafting to improve affinity with the inorganic nanofiller and accelerate hydrolytic degradation. Fourier‐transform infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopies confirmed the occurrence of chemical functionalization. Preliminary characterization of films of PLA, PLAf and relative nanocomposites through water contact angle measurements highlighted an increase of wettability for PLAf, due to the hydrophilic groups grafted onto polymer chain. Thermal analysis showed an increase of glass transition temperature (Tg) in PLAf nanocomposites, likely due to enhanced matrix‐nanoparticle interactions. Scanning electron microscopy (SEM) analysis revealed more defined and homogeneous fibers for PLAf‐HA5 and PLAf‐HA10, meanwhile results from compression tests indicated improved processability and enhanced mechanical properties of nanocomposite PLAf‐based scaffolds, as evidenced by increased values of Young modulus. Hydrolytic degradation studies in Phosphate Buffered Saline (PBS) solution showed greater weight loss and molecular weight decrease for PLAf, PLAf‐HA5, and PLAf‐HA10, suggesting faster degradation due to increased hydrophilicity. Biological tests with human Mesenchymal Stem Cells (hMSCs) demonstrated that all scaffolds promoted cell proliferation, with PLAf‐HA formulations showing higher effect on cellular behavior in terms of cell growth and alkaline phosphatase (ALP) levels, indicating that chemical functionalization improves cell attachment, proliferation and early osteogenic differentiation.Highlights Functionalization of PLA enhances hydrophilicity and HA affinity. Nanocomposite scaffolds based on PLAf are successfully developed by 3D printing. PLA functionalization enhances ink printability, making more uniform structures. PLAf‐based scaffolds exhibit an accelerated hydrolytic degradation PLAf‐HA scaffolds support cell adhesion and early osteogenic differentiation.

Photochemistry of dissolved organic matter derived from compost.

The extensive application of compost to enhance soil quality highlights the crucial role of dissolved organic matter (DOM) derived from compost in both terrestrial and aquatic ecosystems, influencing carbon cycling and the fate of contaminants. However, the photochemical behavior of compost-derived DOM (DOMCOM) remains poorly understood. In this study, we investigated the photochemical transformation and photoactivity of DOM derived from typical composts produced from cow manure (CDOM) and pig manure (PDOM). The results indicated that the initial CDOM exhibited higher molecular weight, aromaticity, humification, and photoactivity compared to PDOM. Under UV irradiation, both DOMCOM underwent photobleaching and photo-humification, resulting in a decrease in the average molecular weight by 23.68% for CDOM and 3.82% for PDOM, with CDOM being particularly affected. Meanwhile, 2D-COS analysis revealed that the fulvic-like fluorescence fraction was first to respond to photoirradiation in both DOM, followed by the protein-like and microbial humic-like fluorescence fractions, which showed contrasting response trends in CDOM and PDOM. Furthermore, CDOM with a higher concentration of humic-like substances efficiently generated 3DOM*, 1O2 and •OH (4.09×10-8, 1.17×10-8 and 7.05×10-12, respectively) under UV radiation, which were apparently greater than those produced by PDOM (3.30×10-8, 8.38×10-9 and 4.99×10-12, respectively).

Debranched Lentil Starch-Sodium Alginate-Based Encapsulated Particles of Lacticaseibacillus rhamnosus GG: Morphology, Structural Characterization, In Vitro Release Behavior, and Storage Stability.

Starches with different degrees of debranching (DBS30, DBS60, and DBS90) and sodium alginate were used as the wall material for encapsulating particles of Lacticaseibacillus rhamnosus GG (LGG). The structural characteristics of these encapsulated particles were examined, along with the impact of varying levels of debranching on the encapsulation efficiency, the in vitro release of LGG under the simulated gastrointestinal environment, and the storage stability of the encapsulated particles. The results revealed a transformation in the crystalline polymorph from C- to B+V-type following debranching and retrogradation. This process also resulted in a significant decrease in molecular weight and polydispersity index, accompanied by an increase in amylose and resistant starch levels along with the relative crystallinity of the debranched lentil starch. Comparatively, DBS60-LGG and DBS90-LGG exhibited higher encapsulation efficiency and encapsulation yield than UDBS-LGG and DBS30-LGG. Furthermore, these encapsulated particles provided enhanced protection for LGG in both the simulated gastrointestinal environment and the storage process. It can be inferred that a superior encapsulation performance of the debranched lentil starch-sodium alginate-based encapsulated LGG particles was associated with higher debranching levels, a more uniform molecular weight distribution, and a more ordered multi-scale structure of the debranched lentil starch.

Effects of interaction between wheat bran dietary fiber and gluten protein on gluten protein aggregation behavior.

Effects of wheat bran dietary fiber (WBDF) as a nutritional additive on flour products quality mainly depends on the interaction between WBDF and gluten protein. In this study, the effects and mechanisms of WBDF with different particle sizes and additive amounts on gluten protein aggregation behavior were investigated. The results showed that the addition of WBDF led to a decrease in free sulfhydryl content, particle size, molecular weight and gluten macromer (GMP) content, an increase in zeta potential and SDS-extractable protein content, and a deterioration in the gluten network morphology compared to the control group, suggesting that the aggregation behavior of gluten protein was inhibited. When WBDF was added at 3% and 6%, dilution effect, mechanical shear, steric hindrance, and non-covalent binding were the main mechanisms leading to depolymerization. Further addition of WBDF (9%, 12%) inhibited the depolymerization of gluten protein due to competitive hydration and non-covalent binding. However, when WBDF was added at 15%, the dilution effect, mechanical shear and steric hindrance of WBDF (88μm<particle size<150μm) dominated, and their inhibitory of aggregation induced the formation of a loose gluten network structure. In contrast, the weaker mechanical shear and steric hindrance effects of WBDF (particle size<88μm) mitigated the degradation of gluten network structures by WBDF.

Comparative study on the physicochemical characteristics of lignin via sequential solvent fractionation of ethanol and Kraft lignin derived from poplar and their applications

Comparative study on the physicochemical characteristics of lignin via sequential solvent fractionation of ethanol and Kraft lignin derived from poplar and their applications

Investigation of the degradation behaviour of poly-L-lactic acid braided stents under real-time and accelerated conditions

Investigation of the degradation behaviour of poly-L-lactic acid braided stents under real-time and accelerated conditions

Phototransformation and photoreactivity of MPs-DOM in aqueous environment: Key role of MPs structure decoded by optical and molecular signatures

The dissolved organic matter (DOM) derived from microplastics (MPs-DOM) can be one of the photoactive components in DOM. However, information on the properties and photoreactivity of MPs-DOM during phototransformation is limited. Here, we investigated the properties and photoreactivity of MPs-DOM from polyolefins (MPs-DOM-POs), MPs-DOM derived from benzene-containing polymers (MPs-DOM-BCPs), and Suwannee River natural organic matter (SR-NOM), during a 168-hour phototransformation. After phototransformation, all examined types of DOM exhibit a decrease in concentration and molecular weight. Notably, MPs-DOM-POs display increased aromaticity and saturation, while MPs-DOM-BCPs and SR-NOM show reduced aromaticity and saturation. MPs-DOM-POs present higher steady-state concentrations of •OH but much lower steady-state concentrations of 1O2 than those of MPs-DOM-BCPs. In comparison, MPs-DOM produce more •OH but less 1O2 than SR-NOM. This study proposes that the diversification of aliphatic C─H bonds (arylation and carbonylation) by reactive intermediates (especially •OH) is the main pathway for MPs-DOM-POs phototransformation for the first time. On the other hand, the cleavage on the aromatic carboxylic acids by reactive intermediates (especially 1O2) is the main mechanism for MPs-DOM-BCPs and SR-NOM phototransformation. Our findings provide new insights into the phototransformation and photoreactivity of MPs-DOM and help to understand the potential risks of MPs in aqueous environment.

Acid resistance of different polysaccharide hydrocolloids: pH effects

Acid resistance of different polysaccharide hydrocolloids: pH effects

Characterization and degradation mechanism of a newly isolated hydrolyzed polyacrylamide-degrading bacterium Alcaligenes faecalis EPDB-5 from the oilfield sludge

Hydrolyzed polyacrylamide (HPAM) is posing serious threats to ecosystems. However, biodegradation is an effective method to remove HPAM owing to its low cost and environmental friendliness. In this study, Alcaligenes faecalis EPDB-5 was isolated as a highly efficient HPAM degrading strain from sludge contaminated with polymerized produced water from Daqing oilfield. Under the optimal conditions, the strain EPDB-5 demonstrated an impressive HPAM degradation rate of 86.05%, the total nitrogen (TN) removal of 71.96% and chemical oxygen demand (COD) removal of 67.98%. Meanwhile, it can maintain a stable degradation rate higher than 75% under different pH and temperature conditions. 27 genes that play a key role in HPAM degradation were annotated by metagenomics sequencing. The key genes were involved in multiple KEGG pathways, including biofilm formation, biosynthesis secondary metabolites, and metabolic pathways. SEM, GPC, and FTIR analyses revealed that the structure of HPAM after biodegradation showed pores, a significant decrease in molecular weight, -NH2 detachment, and carbon chain breakage. Particularly, we propose a possible mechanism of biofilm formation - HPAM degradation - biofilm disappearance and reorganization. Moreover, the degradation rate of strain EPDB-5 on real wastewater containing HPAM was 29.97% in only three days. This work expands our knowledge boundary about the HPAM degradation mechanism at the functional gene level, and supports the potential of strain EPDB-5 as a novel auxiliary microbial resource for the practical application of HPAM.

Enzymatic synthesis of aromatic biobased polymers in green, low-boiling solvents

Given the urge to accelerate the substitution of petrol-derived solvents not only in more traditional fields like pharmaceuticals, personal care, or electronics but also in innovative research processes, this work focuses on the utilisation of four biobased solvents as media for the enzymatic synthesis of aliphatic-aromatic polyesters. As building blocks, the lignin-derived diethyl-2,4-pyridinedicarboxylate was selected as the potentially biobased, aromatic component while more classical diols such as 1,4-butanediol and 1,8-octanediol were used as the aliphatic portion. Results show that among the tested green solvents (cyclohexanone, phenetole, anisole and eucalyptol), the most suitable medium for lipase B from Candida antarctica-catalysed polycondensation reactions was eucalyptol that allowed reach monomer conversions >95 % and number average molecular weights up to 3500 g·mol-1. On the other hand, cyclohexanone led to the lowest monomer conversions (<80 %) and molecular weights (Mn<500 g·mol-1) confirming once again the unsuitability of ketone-containing solvents for enzymatic esterification and transesterification reactions. The lipase could be used up to three times, in eucalyptol as a solvent, without a significant decrease in monomer conversion or molecular weight.

Synthesis and characteristics properties of lignified PVA copolymer with enhanced UV-blocking performance and water solubility

Synthesis and characteristics properties of lignified PVA copolymer with enhanced UV-blocking performance and water solubility

Upcycling of polyethylene waste to biodegradable adhesive and coating via Baeyer-Villiger reaction: A new view on oxidation-fracture mechanism and structure-function relationship

Upcycling of polyethylene waste to biodegradable adhesive and coating via Baeyer-Villiger reaction: A new view on oxidation-fracture mechanism and structure-function relationship

A multi-analytical approach to evaluate the removal efficiency of polystyrene nanoparticles in water treatment processes

The removal of nanoplastics (NP) from water using various treatment processes has gained significant attention recently. This study comprehensively characterizes the degradation of polystyrene nanoparticles (concentration: 200ppm, diameter: 140nm) through UVC irradiation. For the first time, we compared four analytical methods to monitor removal efficiency: Py-GCMS, UV-Visible spectroscopy, TOC, and Turbidity. Additionally, DLS, TEM, and SEC were used to understand changes in particle size, morphology, and molecular weight. Results showed that Py-GCMS overestimated the removal rate by a factor of 2 compared to Turbidity and UV-Visible measurements, which were in agreement. Furthermore, after 200h of irradiation, the styrene signal disappears from the pyrogram, although the mineralization rate reaches only 50%, as determined by total organic carbon (TOC) analysis. The particle size decreased slowly, reaching 100nm after 150h, while a significant decrease in molecular weight indicated high chain-scission. These findings emphasize the importance of a multi-analytical approach to accurately assess NP removal efficiency and understand degradation mechanisms.

Lysine 473 Regulates the Progression of SLC7A11, the Cystine/Glutamate Exchanger, through the Secretory Pathway.

System xc-, the cystine/glutamate exchanger, is a membrane transporter that plays a critical role in the antioxidant response of cells. Recent work has shown that System xc- localizes to the plasma membrane during oxidative stress, allowing for increased activity to support the production of glutathione. In this study, we used site-directed mutagenesis to examine the role of C-terminal lysine residues (K422, K472, and K473) of xCT (SLC7A11) in regulating System xc-. We observed that K473R exhibits loss of transporter activity and membrane localization and is 7.5 kD lower in molecular weight, suggesting that K473 regulates System xc- trafficking and is modified under basal conditions. After ruling out ubiquitination and neddylation, we demonstrated that unlike WT xCT, K473R lacks N- and O-glycosylation and is sequestered in the endoplasmic reticulum. Next, we demonstrated that K473Q, a constitutively acetylated lysine mimic, also exhibits loss of transporter activity, decreased membrane expression, and a 4 kD decrease in molecular weight; however, it is N- and O-glycosylated and localized to the endoplasmic reticulum and Golgi. These results suggest that acetylation and deacetylation of K473 in the endoplasmic reticulum and Golgi, respectively, serve to regulate the progression of the transporter through the biosynthetic pathway.

The role of duel hydrolysis of soybean on functional properties and protein digestibility: a sustainable approach.

Protein hydrolysates derived from food sources contains enormous number of peptides which are composed of amino acid possessess various bioactive properties. However, the use of protein hydrolysates as a nutraceutical is hindered due to their unpleasant flavour. The study aims to enhance the biological activity and palatability of protein hydrolysates. In the present study, soybean protein hydrolysate (SPH) was prepared using alcalase for 4 h (control). Modification of hydrolysis (MPH) was carried out by reiterating the hydrolysis of the supernatant obtained after 2 h of hydrolysis using an enzyme to 50% of alcalase during each successive hydrolysis. Samples were characterised by their physio-chemical and functional properties. Furthermore, the effect of modification on the protein digestibility and bitterness intensity using e-tongue was studied. The suppressive effect on retrogradation of corn starch was analysed using texture profile analysis. The results demonstrated increased protein content by 1.6 and 1.9% in MPH compared to SPH and UNH, respectively. MPH showed 1.5- and 1.6-fold higher DH% than SPH before and after gastrointestinal digestion (p < 0.05). A decrease in molecular weight was found in the order of UNH > SPH > MPH. Nevertheless, MPH displayed significantly higher functional properties (p ≤ 0.05). The hardness of retrograded corn starch was significantly reduced in the MPH (1.21N) than SPH (1.55 N) and UNH (1.81N) compared to control (1.71N) during 7-day storage at 4°C (p ≤ 0.05). E-tongue analysis of MPH showed a 4-fold reduction in bitterness than SPH. Modification of hydrolysis of soybean has demonstrated its significance in improved DH% functional properties and palatability. In addition, improved protein digestibility with promising benefits in deferral action on retrogradation of starch over the traditional process of hydrolysis was observed. The outcome of this study contributes to the potential utilisation of MPH as an ingredient in the formulation of nutraceutical products.

Structure-property correlations study in biochar-enhanced polyamide composites for sustainable materials development

Structure-property correlations study in biochar-enhanced polyamide composites for sustainable materials development

Accelerated hydrolytic degradation of poly(l-lactide) by blending with poly(ether-block-amide)

A continuing challenge in the most common biodegradable polyester of poly(l-lactide) (PLLA) is to improve the degradation rate in the environment, though it has been widely used in packaging and medical applications. In this study, PLLA/poly(ether-block-amide) (PEBA) blends are prepared by melt blending to investigate the effect of PEBA component on the phase morphology, thermal behavior, mechanical properties, and hydrolytic degradation of the blends. The incorporation of PEBA component is beneficial to the improved toughness and increased water absorption of the blends, and accelerated hydrolytic degradation of PLLA. The blend exhibits the optimal mechanical and hydrolytic degradation properties when the blend mass ratio of PLLA/PEBA is 80/20. The toughness of the blend is increased by 390% compared to that of pure PLLA. After being hydrolyzed at 58°C for 240h, the water absorption, the mass loss and the decrease of molecular weight of the blend is increased by 138%, 160% and 40%, respectively, indicating faster hydrolytic degradation rate of the blend than that of pure PLLA. Furthermore, the accelerated hydrolytic degradation mechanism of PLLA in the blend is revealed. The amorphous region of PLLA is hydrolyzed initially at the phase interface of the blend, and subsequently the crystalline structure of PLLA is degraded. The hydrolysis process causes a change in the relative content of crystalline regions in the system, resulting in an increase in crystallinity of PLLA first and then decrease. These findings provide a new strategy for the design of novel degradable PLLA materials for practical applications.