In recent years, carbon nanotubes have been extensively explored based on numerous applications in fields such as carbon nanotube based electrodes, super capacitors, nanowires, sensors, coating with polymers, biomedical, and mechanical applications. Keeping in view the significance of carbon nanotubes in the heat transfer process in micro-electrochemical systems, this study is conducted to examine the heat transfer attributes of the carbon nanotubes using the mixture base working fluid. The focus of this investigation is to elaborate the consequence of higher chemical reactions on the bioconvective flow of carbon nanotubes. Additionally, modified Buongiorno's nanofluid model has been used, which undertakes thermophoresis and Brownian motion effects. Heated boundary condition has also been incorporated with the migration of motile microorganisms. The hybrid nanofluid mechanism has been followed in this work. Carbon nanotubes of single-layer and multi-layer have been used simultaneously with different working fluids, such as ethylene glycol (EG) and ethylene glycol/water (EG-water). Non-dimensional flow model equations have been tackled with the bvp-4c package. The obtained outcomes have been presented for distinct profiles and tabulated data sets for the surface skin friction and heat transfer coefficient. It has been observed that when the level of Brownian motion increases, the velocity profile decreases abruptly for SWCNT-MWCNT/EG as compared to SWCNT-MWCNT/EG-water hybrid nanofluid. Opposite behavior has been seen with raising values of magnetization. High Prandtl number decrease thermal boundary layer thickness for both types of hybrid nanofluids. The motile microorganism profile expands by raising the level of bioconvective Lewis number. Moreover, the outcomes of drag force and Nusselt number have been compared, and good agreement has been found.