Abstract In this paper, a methodology for model parameter calibration for vibration fatigue analysis is proposed. It combines Bayesian updating of uncertain model parameters and artificial neural networks (ANNs). The calibrated parameters are used to increase the accuracy of fatigue lifetime calculations for components submitted to vibrational loads. The Bayesian updating uses eigenfrequencies, mode shapes, total mass, and the frequency response functions (FRFs). These quantities are predicted by ANN-based surrogate models to accelerate the Bayesian updating process. A novel strategy for the prediction of the magnitude and phase of FRFs with ANNs is proposed. The frequency is used as an additional input variable, and a schematic selection of significant points of the FRF curves is presented. A high prediction accuracy of the surrogate models could be achieved. The procedure includes the analysis of the relevant frequency range and a sensitivity analysis based on the Morris method to identify appropriate modes and the most-influential parameters. The proposed framework is applied to a current vehicle component subjected to vibrational loads. An experimental modal analysis is used for the calibration and consideration of real parameter uncertainty. First, the accuracy of the surrogate models and Bayesian updating is verified by a nominal reference simulation and then validated with experimental data. The measurable control parameter thickness and component mass are used to examine the calibration accuracy. Finally, a decrease in the dispersion of the vibration fatigue distribution is obtained with the calibrated parameters.