In the last twenty years, many of jamu or herbal medicine were adulterated with chemical drugs. One of the chemical drugs that is commonly added to herbal medicine is acetaminophen. Acetaminophen is generally considered as an analgesic-antipyretic drug. The sample of this study consisted of 6 samples of jamu or herbal medicine with different brands. Qualitative analysis was conducted by TLC method using the mobile phase n-hexane: ethyl acetate (1:1), it was obtained three positive samples containing acetaminophen, namely sample D, E, and F. The three positive samples containing acetaminophen were calculated using the LCMS method. Quantitative analysis was conducted with LCMS using a reversed-phase with a mobile phase of acetonitrile: water (15%>: 85%> v/v) at a flow rate of 0.2 mL/min and an injection volume of 5 gL, it was obtained the levels of acetaminophen in sample D as much as 0.16 g/2 g of sample, in sample E of 0.63 g/7 g of sample, and in sample F of 0.56 g/7 g of sample. Although the content of acetaminophen in herbal medicine is relatively small, but according to the Regulation of the Minister of Health of Indonesia No. 007 of 2012, traditional medicine or herbal medicine should not contain synthetic chemicals.