Abstract
Objective: In the current investigation, to separated and validate the cancer healing drugs (Carboplatin and Docetaxel) through the HPLC (e-2695) instrument containing a PDA detector. Methods: A simple, selective, validated and well-defined stability that shows isocratic RP-HPLC methodology for the quantitative determination of Carboplatin and Docetaxel. The chromatographic strategy utilized Symmetry C18 column of dimensions 150x4.6 mm, 3.5 micron, using isocratic elution with a mobile phase of acetonitrile and 0.1% ortho phosphoric acid (40:60). A flow rate of 1 ml/min and a detector wavelength of 225 nm utilizing the PDA detector were given in the instrumental settings. Recovery, specificity, linearity, accuracy, robustness, ruggedness were determined as a part of method validation and the results were found to be within the acceptable range. Validation of the proposed method was carried out according to an international conference on harmonization (ICH) guidelines. Results: LOD and LOQ for the two active ingredients were established with respect to test concentration. The calibration charts plotted were linear with a regression coefficient of R2>0.999. Conclusion: The proposed method to be fast, simple, feasible and affordable in assay condition. During stability tests, it can be used for routine analysis of production samples and to verify the quality of drug samples during stability studies.
Highlights
In March 1989, the FDA authorised the medication carboplatin
Carboplatin primarily reacts with alkyl groups on nucleotides [8, 9], which is necessary for DNA fragmentation [10, 11], and simple adducts after restoration enzymes correct the error
We present in this article simple, selective, validated and well-defined stability that shows isocratic RP-HPLC methodology for the quantitative determination of Carboplatin and Docetaxel
Summary
In March 1989, the FDA authorised the medication carboplatin. Paraplatin is the trade name for carboplatin, which comes in injection form (150 mg/ml or 10 mg/ml) and is an organo platinum alkylating representative. Carboplatin is a cancer-fighting (chemotherapy) [1, 2] medication [3] that is used to treat ovarian cancer [4, 5]. The treatment of innovative ovarian cancer with a combination of carboplatin and chemotherapeutic agents. Carboplatin resembles cisplatin in terms of vomiting and nephrotoxicity [6, 7]. Carboplatin primarily reacts with alkyl groups on nucleotides [8, 9], which is necessary for DNA fragmentation [10, 11], and simple adducts after restoration enzymes correct the error. Carboplatin and plasma protein [12] have no affinity for each other. 40% of free platinum, on the other hand, is irreversibly attached to plasma proteins. Patients who are overdue for carboplatin may experience severe hepatotoxicity [13, 14] and neutropenia [15, 16]
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