μM Trolox Research Articles

Anacardium humile St. Hil as a novel source of antioxidant, antiglycation and α-amylase inhibitors molecules with potential for management of oxidative stress and diabetes

Ethnopharmacological relevanceThe substantial increase in diabetes cases worldwide has been a major public health problem, and the use of medicinal plants can be considered an interesting alternative to control the disease and its complications. Anacardium humile St. Hill. (Anacardiaceae) is a typical plant from the Brazilian savanna, popularly known for its antidiarrheal, expectorant, antidiabetic and anti-inflammatory properties, however, few studies have fully described its biological properties. This study aimed to investigate in vitro and ex vivo the antioxidant and antiglycation potential of A. humile ethanolic extract, its organic fractions and three isolated molecules (quercetin, catechin and gallic acid), their capacity to inhibit the glycolytic enzyme α-amylase, as well as their cytotoxic effects against RAW264.7 macrophages. Material and methodsThe ethanolic extract of A. humile, its organic fractions and three isolated molecules (catechin, quercetin and gallic acid) were tested for their antioxidant (ORAC, FRAP and DPPH) and antiglycation (BSA/Fructose, BSA/Methylglyoxal, Arginine/Methylglyoxal and Lysine/Methylglyoxal) capacities, and also for its potential to inhibit the enzyme α-amylase. Additionally, bioactive compounds present in the A. humile leaves fractions were elucidated by an HPLC-ESIMS/MS analysis. ResultsThe analysis showed relevant antioxidant activity of DCM (1264.85 ± 76.90 μM Trolox eq/g ORAC; 216.71 ± 1.04 μM Trolox eq/g FRAP and 3.03 ± 0.08 IC50 μg/mL IC50 DPPH) and EtOAc (1300.11 ± 33.04 ORAC, 236.21 ± 23.86 FRAP and 3.03 ± 0.14 μg/mL IC50 DPPH) fractions and also of the isolated molecules, mainly gallic acid (1291.19 ± 8.41 μM Trolox eq/g ORAC, 1103.52 ± 31.48 μM Trolox eq/g FRAP and 0.78 ± 0.11 μg/mL IC50 DPPH). Concerning the antiglycation activity, all samples inhibited over 88% in the BSA-FRU method. In the BSA-MGO and ARG-MGO methods, the Hex, DCM, EtOAc fractions and the isolated molecule catechin stood out. However, in the LYS-MGO model, only the isolated molecules showed significant results. In α-amylase assay, all fractions, for exception Hex, presented notable inhibition capacity with low IC50 values, especially DCM, EtOAc, ButOH and H2O (IC50 0.56 ± 0.10, 0.84 ± 0.01, 0.74 ± 0.03 and 0.79 ± 0.06 μg/mL, respectively). Tests using hepatic tissue showed a notorious capacity of the DCM, AcOEt and ButOH fractions, as well as of the isolated molecules to inhibit lipid peroxidation and ROS production, and also to preserve thiol groups. Molecules of great antioxidant potential were found in our samples, such as kaempferol, quercetin, catechin, gallic acid and luteolin. ConclusionA. humile extract and its organic fractions showed promising antioxidant and antiglycation potential and a prominent capacity to inhibit the α-amylase enzyme. Hence, this study presents new results and stimulates further research to elucidate the biological properties of A. humile and its capacity to manage DM and its complications.

Application of the nutrient profile model in the development of food bars with functional potential.

In the present study, the nutrient profile model is used to develop functionally potential food bars prepared with pumpkin pulp flour (PuPF) and pequi peel flour (PePF). The control (0% PuPF, 0% PePF), and treatments T1 (0% PuPF, 2.5% PePF), T2 (5% PuPF, 2.5% PePF), T3 (10% PuPF, 2.5% PePF), and T4 (17.5% PuPF, 2.5% PePF) were defined using the nutrient profile model and subsequently evaluated for antioxidant activity, sensory acceptance, and nutrient profile. All treatments were nutritionally balanced but T3 and T4 were the best, containing, respectively, 35.11 and 59.85μgg-1 of carotenoids, 230.60 and 261.14mg gallic acid equivalents 100g-1 of phenolics, and high antioxidant activity, as determined using ABTS (28.60 and 34.86μMTroloxg-1), FRAP (67.13 and 80.09μMferrous sulfateg-1), and β-carotene/linoleic acid system (79.08 and 84.83% protection) methods. The nutrient profile model minimized time and resource expenditures throughout the development process.

Callus Culture of Thai Basil Is an Effective Biological System for the Production of Antioxidants.

Thai basil is a renowned medicinal plant and a rich source of bioactive antioxidant compounds with several health benefits, with actions to prevent of cancer, diabetes and cardiovascular disease. Plant cell and tissue culture technologies can be routinely established as an important, sustainable and low-cost biomass source to produce high-value phytochemicals. The current study aimed at developing an effective protocol to produce Thai basil leaf-derived callus cultures with sustainable and high production of biomass and antioxidants as an alternative of leaves production. MS basal medium with various concentrations of plant growth regulators (PGRs) compatible with nutraceutical applications (i.e., gibberellic acid (GA3) and 6-benzylaminopurine (BAP) either alone or in combination with naphthalene acetic acid (NAA)) were evaluated. Among all tested PGRs, the combination BAP:NAA (5 mg/L:1 mg/L) yields the maximum biomass accumulation (fresh weight (FW): 190 g/L and dry weight (DW): 13.05 g/L) as well as enhanced phenolic (346.08 mg/L) production. HPLC quantification analysis indicated high productions of chicoric acid (35.77 mg/g DW) and rosmarinic acid (7.35 mg/g DW) under optimized callus culture conditions. Antioxidant potential was assessed using both in vitro cell free and in vivo cellular antioxidant assays. Maximum in vitro antioxidant activity DPPH (93.2% of radical scavenging activity) and ABTS (1322 µM Trolox equivalent antioxidant capacity) was also observed for the extracts from callus cultures grown in optimal conditions. In vivo cellular antioxidant activity assay confirmed the effective protection against oxidative stress of the corresponding extract by the maximum inhibition of ROS and RNS production. Compared to commercial leaves, callus extracts showed higher production of chicoric acid and rosmarinic acid associated with higher antioxidant capacity. In addition, this biological system also has a large capacity for continuous biomass production, thus demonstrating its high potential for possible nutraceutical applications.

Antioxidant Potential of Mung Bean (Vigna radiata) Albumin Peptides Produced by Enzymatic Hydrolysis Analyzed by Biochemical and In Silico Methods.

The objective of this study was to investigate the biochemical antioxidant potential of peptides derived from enzymatically hydrolyzed mung bean (Vigna radiata) albumins using an 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, a ferrous ion chelating assay and an oxygen radical absorbance capacity (ORAC) assay. Peeled raw mung bean was ground into flour and mixed with buffer (pH 8.3, 1:20 w/v ratio) before being stirred, then filtered using 3 kDa and 30 kDa molecular weight cut-off (MWCO) centrifugal filters to obtain albumin fraction. The albumin fraction then underwent enzymatic hydrolysis using either gastrointestinal enzymes (pepsin and pancreatin) or thermolysin. Peptides in the hydrolysates were sequenced. The peptides showed low ABTS radical-scavenging activity (90–100 μg ascorbic acid equivalent/mL) but high ferrous ion chelating activity (1400–1500 μg EDTA equivalent/mL) and ORAC values (>120 μM Trolox equivalent). The ferrous ion chelating activity was enzyme- and hydrolysis time-dependent. For thermolysin hydrolysis, there was a drastic increase in ferrous ion chelating activity from t = 0 (886.9 μg EDTA equivalent/mL) to t = 5 min (1559.1 μg EDTA equivalent/mL) before plateauing. For pepsin–pancreatin hydrolysis, there was a drastic decrease from t = 0 (878.3 μg EDTA equivalent/mL) to t = 15 (138.0 μg EDTA equivalent/mL) after pepsin was added, but this increased from t = 0 (131.1 μg EDTA equivalent/mL) to t = 15 (1439.2 μg EDTA equivalent/mL) after pancreatin was added. There was no significant change in ABTS radical scavenging activity or ORAC values throughout different hydrolysis times for either the thermolysin or pepsin–pancreatin hydrolysis. Overall, mung bean hydrolysates produced peptides with high potential antioxidant capacity, being particularly effective ferrous ion chelators. Other antioxidant assays that use cellular lines should be performed to measure antioxidant capacity before animal and human studies.

Carbonic anhydrase enzyme inhibition and biological activities of Satureja hortensis L. essential oil

Recently, there is a great need to discover new natural carbonic anhydrase (CA) inhibitor sources to be used as diuretic, antiglaucoma, anti-obesity, anticancer, and anti-infective drugs. Therefore, evaluation of CA inhibition activity and possible inhibition mechanism of the essential oil of Satureja hortensis L. (SHEO) were investigated for the first time by this study. Additionally, total phenolic content known effective for CA inhibition and the other biological activities was determined for the first time. Since the volatile compound and total phenolic content of essential oil of S. hortensis grown in Bingöl exhibited great differences than those in the literature, the antioxidant and antimicrobial activities of SHEO were also investigated. Carbonic anhydrase inhibition of SHEO was determined as IC50: 0.1 ± 0.002 μg/mL. Further, molecular docking studies between the main volatile compounds and CA have been conducted to better understand the CA inhibition mechanism of SHEO. The main compounds were as β-decalol (19.10%), perillaldehyde (17.11%), perillyl alcohol (16.87%), carvacrol (13.47%), and thymol (12.32%) by GC-MS. β-decalol was detected in an essential oil for the first time. Based on the molecular docking studies, thymol and β-decalol showed great potential for CA inhibition. Besides, total phenolic content was determined as 623 ± 2.88 μg/mL gallic acid equivalent (GAE) and 736 ± 3.46 μg/mL catechin equivalent (CE). The essential oil showed high antioxidant capacity [(SC50: 0.069 ± 0.283 μg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 1031 ± 5.03 μM trolox equivalent antioxidant capacity (TEAC) for ferric reducing power (FRAP)]. The essential oil also showed significant antimicrobial activity against six Gram-positive and six Gram-negative bacteria, and a fungus. Minimal inhibitory concentrations (MICs) were 15.6 μg/mL and 31.2 μg/mL for MRSA ATCC 67101 and C. albicans, respectively. The rest of eleven bacteria exhibited MICs between 3.9-7.8 μg/mL. Consequently, SHEO could be used as antioxidant and antimicrobial agent at very low concentrations. In addition, SHEO significantly inhibited CA, thus, SHEO was a promising source for CA inhibitors and it might have potential to be used for treatment of CA related diseases.

Microorganisms and Biostimulants Impact on the Antioxidant Activity of Buckwheat (Fagopyrum esculentum Moench) Sprouts.

The study analyzes the influence of plant growth promoters and biological control agents on the chemical composition and antioxidant activity (AA) in the sprouts of buckwheat. The AA of cv. Kora sprouts was higher than cv. Panda, with 110.0 µM Fe2+/g (FRAP—Ferric Reducing Antioxidant Power), 52.94 µM TRX (Trolox)/g (DPPH—1,1-diphenyl-2-picrylhydrazyl), 182.7 µM AAE (Ascorbic Acid Equivalent)/g (Photochemiluminescence—PCL—ACW—Water-Soluble Antioxidant Capacity) and 1.250 µM TRX/g (PCL—ACL—Lipid-Soluble Antioxidant Capacity). The highest AA was found in the sprouts grown from seeds soaked in Ecklonia maxima extract and Pythium oligandrum (121.31 µM Fe2+/g (FRAP), 56.33 µM TRX/g (DPPH), 195.6 µM AAE/g (PCL—ACW) and 1.568 µM TRX/g (PCL—ACL). These values show that the antioxidant potential of buckwheat sprouts is essentially due to the predominant hydrophilic fraction of antioxidants. The AA of the sprouts was strongly correlated with total polyphenol content.

Potential enzyme inhibitory properties of extracts and fractions from fruit latex of Ficus carica-based on inhibition of α-amylase and α-glucosidase

Ficus L. is one of the widespread genera of the Moraceae family. We aimed to evaluate the inhibiting effect on digestive enzymes linked to diabetes, the antioxidant activity and the phytochemical composition of active extract/fraction of fruit latex of Ficus carica L. subsp. carica (All.) Schinz Et Thel. The inhibition effect of extracts and fractions tested with α-amylase and α-glucosidase methods and the antioxidant effect investigated with ABTS·+ and FRAP tests. The qualitative determination of compounds within the active extract/fraction was carried out by LC–MS/MS. In a result, the highest α-amylase and α-glucosidase activity found in F2 fraction of fruit latex (IC50 = 195.205 ± 0.015 µg/mL and 6.920 ± 0.026 µg/mL, respectively). Moreover, the highest total phenolic (26.816 ± 0.007 mgGAE/gextract) and total flavonoid content (8.615 ± 0.009 mgCA /gextract) was found in S1extract and this extract showed the highest antioxidant activity on FRAP (at a concentration of 1 mg/mL equivalent to 179.802 ± 2.201 mmol Fe2+) and ABTS+· (at a concentration of 1 mg/mL equivalent to 0.296 ± 0.055 µM Trolox) tests. Besides that, a preliminary examination of the mass spectrums of active F2 fraction and S1 extract revealed the presence of one xanthone, one organic acid derivative, one chromone derivative, one fatty acid and one phenolic acid. It is the first report on α-amylase, α-glucosidase and antioxidant activities, and phytochemical analysis of fruit latex of F. carica subsp. carica and these studies could contribute to the advanced pharmacological activity studies of this species.

Modifying alcalase activity and stability by immobilization onto chitosan aiming at the production of bioactive peptides by hydrolysis of tilapia skin gelatin

The protease from Bacillus licheniformis, commercially known as Alcalase®, was insolubilized and stabilized by immobilization onto activated chitosan. Activation with different agents, such as glutaraldehyde (GLU-Chi), glyoxyl (GLY-Chi) and divinyl sulfone (DVS-Chi) was investigated. The effect of the immobilization protocol, for instance different pH and times, were also evaluated. GLU-Chi showed the highest activity (35.6UNPA/g) with the smallest substrate (N-Boc-l-alanine p-nitrophenyl-ester, NPA), while GLY-Chi showed the highest activity (1.5 UAzocasein/g) using the greatest substrate (azocasein). A 24-h immobilization period was enough to stabilize the enzyme using the three supports under almost all conditions. Operational stability in azocasein hydrolysis was assayed and GLU-Chi showed no activity loss during 5 cycles. DVS-Chi retained around 70 % of its initial activity after the fifth cycle, whereas GLY-Chi activity retained only 10 %. Finally, the biocatalysts were used in the hydrolysis of tilapia skin gelatin aiming the production of peptides with antioxidant activity. The protein hydrolysates obtained using GLU-Chi presented the highest antioxidant activity (36.7 μM Trolox Eq). However, the best results of operational stability were obtained using DVS-Chi, which did not lose its initial activity after 3 consecutive cycles of gelatin hydrolysis.

Effect on Adipose Tissue of Diabetic Mice Supplemented with n-3 Fatty Acids Extracted from Microalgae.

BackgroundType 2 Diabetes Mellitus (T2DM) is considered a chronic noncommunicable disease in which oxidative stress is expected as a result of hyperglycaemia. One of the most recent approaches is the study of microalgae fatty acids and their possible antioxidant effect.ObjectiveThis study aimed to analyse the effect of supplementation with n-3 fatty acids extracted from microalgae on the total antioxidant capacity (TAC) and lipid peroxidation of adipose tissue and plasma from diabetic (db/db) and healthy (CD1) mice.MethodsMice were supplemented with lyophilized n-3 fatty acids extracted from microalgae or added to the diet, from week 8 to 16. TAC assay and Thiobarbituric Acid Reactive Substances assay (TBARS) were performed on adipose tissue and plasma samples.ResultsThe supplementation of lyophilized n-3 fatty acids from microalgae increased the total antioxidant capacity in adipose tissue of diabetic mice (615.67μM Trolox equivalents vs 405.02μM Trolox equivalents from control mice, p<0.01) and in the plasma of healthy mice (1132.97±85.75μM Trolox equivalents vs 930.64±32μM Trolox equivalents from modified diet mice, p<0.01). There was no significant effect on lipid peroxidation on both strains.ConclusionThe use of n-3 fatty acids extracted from microalgae could be a useful strategy to improve total antioxidant capacity in T2DM.

Dual impact of different drying treatments and ethanol/water ratios on antioxidant properties and colour attribute of jackfruit leaves (Artocarpus heterophyllus Lam.) Mastura variety (J35)

Artocarpus heterophyllus (jackfruit) leaves (JL) are a waste product that is commonly used as livestock feed. Jackfruit leaves have been revealed to possess many medicinal values such as antioxidant and anti-inflammatory properties. In this study, different drying treatments (shade (SD), sun (SN), and oven (OV)) and ethanol/water ratios (E/W) were investigated to evaluate the impact on drying kinetics, color, and antioxidant properties of jackfruit leaves. Results showed that the Newton model was the best fitted mathematical model for the JL drying kinetics. The moisture effective diffusivities ranged from 2.920 × 10-10 to 6.814 × 10-10 m2/s over the temperature range studied. Shade drying was able to preserve the green pigment better than OV and SN drying treatments. Treatment with ethanol/water ratio at 80% and oven-dried (OV80) revealed the highest phenolic content (195.05 ± 1.21 mg gallic acid equivalent (GAE)/g extract weight (EW)), flavonoid content (11.02 ± 0.17 mg artocarpin equivalent (AE)/g EW), and antioxidant activities (90% scavenging activity and reducing power of 1043.84 ± 5.28 µM trolox equivalent (TE)/g EW) compared to SD and SN treatments. The OV80 also possessed the highest artocarpin, squalene, and β-sitosterol contents determined. The OV80 was selected for improving antioxidant and colour stability, and has the potential to be developed into functional biopolymer production.

Biochemical and functional properties of wheat middlings bioprocessed by lactic acid bacteria.

The present study aimed to investigate the bioprocessing of wheat middlings with different lactic acid bacteria (LAB) in order to improve biological activities of this by-product of wheat flour production. The concentration of lactic acid, reducing sugars, and total phenolics, as well as antioxidant, antibrowning, antibacterial and prebiotic activities of fermented samples were analyzed. All LAB strains were capable to growth on wheat middlings, and pH decreased in the medium associated with lactic acid production during cultivation. Samples inoculated with Lactobacillus plantarum DSM20174 presented the maximum growth, lactic acid concentration above 2mg/ml, and pH values around 3.8. The amount or reducing sugars decreased after 24hr growth, except for maltose. Bioprocessed wheat middlings exhibited antioxidant, antibrowning, antibacterial, and prebiotic properties, related with the increase of total phenolic content. Highest values for antioxidant activities were obtained for L. plantarum and Streptococcus thermophilus strains, reaching values around 400 and 640 μM Trolox equivalents (TE) ml-1 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) assays, respectively. Bioprocessing techniques using LAB can be an interesting approach to improve the availability of compounds with health-promoting properties from lignocellulosic waste material. PRACTICAL APPLICATIONS: The processing of secondary products from wheat milling can represent an important benefit to the industry. Wheat middlings bioprocessed with LAB showed improved biological activities and may represent an interesting ingredient to be incorporated in food and feed formulations.

Oxidative Stress Modulation by Cameroonian Spice Extracts in HepG2 Cells: Involvement of Nrf2 and Improvement of Glucose Uptake.

Oxidative stress plays a relevant role in the progression of chronic conditions, including cardiometabolic diseases. Several Cameroonian plants, including spices, are traditionally used as herbal medicines for the treatment of diseases where oxidative stress contributes to insulin resistance, like type 2 diabetes mellitus. This study evaluated the antioxidant capacity and the effects on oxidative-stress-induced impairment of glucose uptake of 11 Cameroonian spice extracts. H2O2-induced reactive oxygen species (ROS) production by human HepG2 cells was significantly reduced by 8/11 extracts. The most effective extracts, Xylopia parviflora, Echinops giganteus, and Dichrostachys glomerata, showed a concentration-dependent ROS-scavenging activity, which involved Nrf2 translocation into the nucleus. Xylopia parviflora, Tetrapleura tetraptera, Dichrostachys glomerata, Aframomum melegueta, and Aframomum citratum extracts showed the highest antioxidant capacity, according to oxygen radical absorbance capacity (ORAC) (2.52–88 μM Trolox Eq/g of extract), ferric-reducing antioxidant power (FRAP) (40.23–233.84 mg gallic acid Eq/g of extract), and total phenol (8.96–32.96% mg gallic acid Eq/g of extract) assays. In HepG2 cells, glucose uptake was stimulated by 4/11 extracts, similarly to insulin and metformin. H2O2-induced oxidative stress reduced glucose uptake, which was rescued by pretreatment with Xylopia aethiopica, Xylopia parviflora, Scorodophloeus zenkeri, Monodora myristica, and Dichrostachys glomerata extracts. The ROS-scavenging ability of the spice extracts may reside in some secondary metabolites observed by phytochemical profiling (reverse-phase high-performance liquid chromatography coupled to a diode array detector (HPLC-UV-DAD)). Further studies are needed to better clarify their biological activities and potential use to control oxidative stress and promote insulin sensitivity.

Food-Safe Process for High Recovery of Flavonoids from Cocoa Beans: Antioxidant and HPLC-DAD-ESI-MS/MS Analysis.

Considering the increasing interest in the incorporation of natural antioxidants in enriched foods, this work aimed to establish a food-grade and suitable procedure for the recovery of polyphenols from cocoa beans avoiding the degreasing process. The results showed that ultrasound for 30 min with particle sample size < 0.18 mm changed the microstructure of the cell, thus increasing the diffusion pathway of polyphenols and avoiding the degreasing process. The effect of temperature, pH, and concentration of ethanol and solute on the extraction of polyphenols was evaluated. Through a 24 full factorial design, a maximum recovery of 122.34 ± 2.35 mg GAE/g, 88.87 ± 0.78 mg ECE/g, and 62.57 ± 3.37 mg ECE/g cocoa beans, for total concentration of polyphenols (TP), flavonoids (TF), and flavan-3-ols (TF3), respectively, was obtained. Based on mathematical models, the kinetics of the solid–liquid extraction process indicates a maximum equilibrium time of 45 min. Analysis by HPLC-DAD-ESI-MS/MS showed that our process allowed a high amount of methylxanthines (10.43 mg/g), catechins (7.92 mg/g), and procyanidins (34.0 mg/g) with a degree of polymerization >7, as well as high antioxidant activity determined by Oxygen Radical Absorbance Capacity (1149.85 ± 25.10 µMTrolox eq/g) and radical scavenging activity (DPPH•, 120.60 ± 0.50 µM Trolox eq/g). Overall, the recovery method made possible increases of 59.7% and 12.8% in cocoa polyphenols content and extraction yield, respectively. This study showed an effective, suitable and cost-effective process for the extraction of bioactive compounds from cocoa beans without degreasing.

Comparative Analysis of Commercial Chinese Bean Sauce (Chunjang) from Korea and China Based on Antioxidant Activity.

In this study, the antioxidant effects of seven types of commercial Chunjang from China (C1∼3) and Korea (K1∼4) were compared for their ability to protect against H2O2-induced DNA damage. Outputs included total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH RSA), oxygen radical absorbance capacity (ORAC), total radical trapping antioxidant potential (TRAP), and comet assays. TPC was the highest in C3 (1,250.8 mg gallic acid equivalent/100 g). C1 exhibited a significantly higher DPPH RSA IC50 (7.5 mg/mL) and ORAC concentration (8.22 μM Trolox equivalent) compared with all other samples, and C1 and K1 exhibited the highest TRAPs. H2O2-induced DNA damage was effectively protected by Chunjang, with a higher observed in C2, C3, and K1 (24.2∼25.3 μg/mL) compared with the other samples (28.3∼30.0 μg/mL). Our results showed that commercial Chunjang contains polyphenol and antioxidant activities. The differences between the samples might be attributed to different origins, materials, and processing methods.

Antioxidant Potential of Antiviral Drug Umifenovir.

Free radical reactions play an important role in biological functions of living systems. The balance between oxidants and antioxidants is necessary for the normal homeostasis of cells and organisms. Experimental works demonstrate the role of oxidative stress that is caused by influenza virus as well as the toxic effects of some antiviral drugs. Therefore, antiviral drugs should be characterized by its pro- and antioxidant activity, because it can affect its therapeutic efficiency. The aim of the study was to quantify the antioxidant capacity and propose the mechanism of the antioxidant effect of the antiviral drug Umifenovir (Arbidol®). The kinetic chemiluminescence with the 2,2’-azobis (2-amidinopropane) dihydrochloride + luminol system was used to quantify the antioxidant capacity of Umifenovir relative to the standard compound Trolox. With computer simulation, the reaction scheme and rate constants were proposed. The antioxidant capacity of 0.9 μM Umifenovir (maximum concentration of Umifenovir in blood after oral administration of 200 mg) was as high as 1.65 ± 0.18 μM of Trolox. Thus, the total antioxidant capacity of Umifenovir is comparable to the antioxidant capacity of Trolox. Unlike Trolox, Umifenovir reacts with free radicals in two stages. For Trolox, the free radical scavenging rate constant was k = 2000 nM−1 min.−1, for Umifenovir k1 = 300 nM−1min.−1, k2 = 4 nM−1min.−1. Slower kinetics of Umifenovir provides the prolonged antioxidant effect when compared to Trolox. This phenomenon can make a serious contribution to the compensation of oxidative stress that is caused by a viral disease and the therapeutic effect of the drug.

Volatile profiling and UHPLC-QqQ-MS/MS polyphenol analysis of Passiflora leschenaultii DC. fruits and its anti-radical and anti-diabetic properties

Twenty-four phenolic compounds including daidzein, epicatechin and artepillin C were identified in Passiflora leschenaultii DC. fruit by UHPLC-QqQ-MS/MS analysis. The aroma profile has been studied using the HS–SPME/GC–MS which revealed 67 volatile compounds including 13 terpenes, 18 alcoholics, 15 esters, ketones and phenolic acids. Further, the proximate composition, anti-radical and anti-diabetic activities of fruit pulp were also determined. The fresh fruit pulp of P. leschenaultii registered higher total phenolic (691.90 mg GAE/g extract) and tannin (313.81 mg GAE/g extract) contents and it also exhibited maximum DPPH (IC50 of 6.69 µg/ml) and ABTS+ (9760.44 µM trolox equivalent/g extract) scavenging activities. The fresh fruit pulp showed a strong inhibition towards the α-Amylase and α-Glucosidase (IC50 of 32.20 and 19.81 µg/mL, respectively) enzymes. Thus, the work stipulates that phenolic compounds rich P. leschenaultii fruit can serve as a potential nutraceutical, antioxidative and anti-diabetic agent in food and pharmaceutical formulations.

Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice.

The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 20–25 g were randomly assigned to seven groups: Control (CON); scopolamine (SCO, 1 mg/kg B.W., intraperitoneally (i.p.); tetrahydroaminoacridine 10 [THA 10, tacrine; 10 mg/kg B.W. per oral (p.o.) with SCO (i.p.)]; PSW 10 (10 mg/kg B.W. (p.o.) with SCO (i.p.); PSW 40 (40 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 100 (100 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 400 (400 mg/kg B.W. (p.o.) with SCO (i.p.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 μM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly affect on physical performance of mice. LPSW 400 significantly increased spontaneous alternation, reaching the level observed for THA and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine (ACh) content and decreased ACh esterase activity (p<0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent.

Urolithin A is a metabolite generated from ellagic acid and ellagitannins by the intestinal microbiota after consumption of fruits such as pomegranates or strawberries. The objective of this study was to determine the cytoprotective capacity of this polyphenol in Neuro-2a cells subjected to oxidative stress, as well as its direct radical scavenging activity and properties as an inhibitor of oxidases. Cells treated with this compound and H2O2 showed a greater response to oxidative stress than cells only treated with H2O2, as mitochondrial activity (MTT assay), redox state (ROS formation, lipid peroxidation), and the activity of antioxidant enzymes (CAT: catalase, SOD: superoxide dismutase, GR: glutathione reductase, GPx: glutathione peroxidase) were significantly ameliorated; additionally, urolithin A enhanced the expression of cytoprotective peroxiredoxins 1 and 3. Urolithin A also acted as a direct radical scavenger, showing values of 13.2 μM Trolox Equivalents for Oxygen Radical Absorbance Capacity (ORAC) and 5.01 µM and 152.66 µM IC50 values for superoxide and 2,2-diphenyss1-picrylhydrazyl (DPPH) radicals, respectively. Finally, inhibition of oxidizing enzymes, such as monoamine oxidase A and tyrosinase, was also detected in a dose-dependent manner. The cytoprotective effects of urolithin A could be attributed to the improvement of the cellular antioxidant battery, but also to its role as a direct radical scavenger and enzyme inhibitor of oxidases.

Wine grape pomace flour in broiler diets effects growth and some meat characteristics

Context Grape pomace maybe useful in broiler diets as a source of low cost antioxidants. Aims The objective of this work was to determine the effect of including high concentrations of wine-grape pomace flour (WGPF) in broiler chicken diets on productive parameters and antioxidant capacity of the meat. Methods WGPF of white (WGPF-W) and red (WGPF-R) grape varieties were nutritionally and chemically characterised. Then, 120 broiler chickens were allocated to three isoenergetic and isoproteic feeding treatments: 0% WGPF (Control), 20% WGPF-W and 20% WGPF-R. Key results WGPF-W had no effect on bodyweight, daily weight gain, feed intake or feed conversion ratio (FCR). However, FCR was higher for WGPF-R treatment at the end of the study (Day 42). Meanwhile, breast meat from WGPF-R treatment had the highest content of ether extract (P &amp;lt; 0,05), followed by WGPF-W and by control treatment, due to the addition of higher amounts of soy oil to those diets with WGPF to ensure an isoenergetic composition. Breast and leg meat, respectively, showed greater antioxidant capacity (µM Trolox Eq/g) when WGPF-W (16.7 and 16.4) was fed, than the antioxidant capacity obtained for control (13.8 and 13.8) and WGPF-R (11.9 and 14.2) treatments. Conclusions Inclusion of 20% of WGPF-W increased antioxidant capacity of chicken meat by 17%, without decreasing productive parameters, provided the diets were made isoenergetic and isoproteic by adding soy oil. Implications The grape pomace flour could be useful in the diet of other animals.

In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss.

Context: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH●, ABTS+● and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 ± 1.088 mg gallic acid equivalent/gextract) and total flavonoid content (86.978 ± 0.650 mg catechin equivalent/gextract) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH● (IC50 = 0.264 ± 0.040 mg/mL), ABTS+● (at a concentration of 0.125 mg/mL equivalent to 0.497 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 1476 ± 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 ± 0.703 µg/mgextract), chlorogenic acid (80.405 ± 0.170 µg/mgextract), isorhamnetin 3-O-rutinoside (72.280 ± 0.33 µg/mgextract), rutin (63.020 ± 0.052 µg/mgextract) and caffeic acid (55.153 ± 0.523 µg/mgextract). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents.