Patinopecten Yessoensis Research Articles

Antioxidant activity of Yesso scallop (Patinopecten yessoensis) female gonad hydrolysates-ribose Maillard reaction products extracted with organic reagents, before and after in vitro digestion

Yesso scallop (Patinopecten yessoensis) gonad is the main by-product during processing. Our previous studies found that its Alcalase hydrolysates can act as a worthy precursor for Maillard reaction in the presence of ribose. Basing on the understanding of the characteristics of scallop female gonad hydrolysates (SFGHs)-ribose Maillard reaction products (MRPs), it was further extracted by different organic reagents and then was subjected to an in vitro digestion. The effects of digestion on the antioxidant and antimicrobial capacities of organic extracts from SFGHs-ribose MRPs were determined. Results showed that the scavenging activities of ethanol and methanol extracts on both ABTS·+ and DPPH·+ increased significantly before digestion, where methanol extracts were better than that of ethanol. However, the ·OH scavenging and linoleic acid peroxidation inhibition activities of MRPs were reduced in both extracts. Interestingly, there was a significant increase in the ABTS·+, ·OH scavenging activities and peroxidation inhibition capacity for all samples following digestion except for DPPH·+ scavenging activity. MRPs-methanol extracts showed the highest antimicrobial effect against Escherichia coli (E. coli) than other samples. Moreover, the HepG2 cell viability of MRPs-methanol extracts after digestion was increased to 89.58 %, which was 1.49, 1.26 and 1.14 times higher than H2O2-treated, SFGHs and MRPs group, respectively. Therefore, it is suggested that methanol extraction can improve the antioxidant capacity of SFGHs-ribose MRPs during digestion, and the corresponding extracts can be utilized as food antioxidants.

Genome-wide identification and characterization of Pif genes in the Yesso scallop (Patinopecten yessoensis), and their expression patterns in response to Polydora shell infestation

Pif is an acid matrix protein that is known to be a key macromolecule in the shell nacre formation of molluscs. However, the investigation into the Pif gene at the whole genome level has seldom been carried out, limiting the systematic understanding of its evolution and function. The Yesso scallop (Patinopecten yessoensis), an ancient bivalve with important economic value and evolutionary status, has suffered severe shell infestation by Polydora that badly damaged shell structures. In this study, a total of eight Pif homologous genes (PyPif1–7 and PyBMSP) were identified in the whole genome of the Yesso scallop with diverse gene structures. Two conserved functional domains, VWA and chitin-binding domains, were detected in all PyPif proteins, although with variation in number of copies. Phylogenetic analysis of the Pifs among different molluscan species suggested the molecular evolution of Pif genes was consistent with taxonomic relationships. High sequence divergence of Pif proteins existed among the different species, but a high level of similarity was found in BMSPs, which own four VWA domains. High expression levels of PyBMSP in the central mantles of the healthy and Polydora shell infested Yesso scallops indicated its important role in shell formation. The other PyPifs (PyPif2, PyPif3, PyPif4, PyPif5, PyPif6) may function when the shell is injured, as significantly increased expression levels of these genes were found in the edge mantles of the infested scallops. In addition, haemocytes may also participate in shell repair by up-regulating the expression of PyPifs. This study provides new insights into the evolution and expression patterns of Pif genes in molluscs, which are valuable for elucidating the mechanism of shell repair and formation.

Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis.

Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines.

The expression profile of calnexin in Patinopecten yessoensis after acute high temperature stress

Calnexin (CNX) is one of the major calcium-binding proteins in endoplasmic reticulum (ER) and plays a crucial role in regulating Ca2+ homeostasis and unfolded protein response (UPR). In the present study, PyCNX was identified in Yesso Scallop Patinopecten yessoensis. The open reading frame (ORF) of PyCNX was of 1794 bp encoding a putative polypeptide of 598 amino acid residues with an N-terminal signal peptide, a typical calreticulin (CRT) domain and a C-terminal transmembrane domain. The deduced amino acid sequence of PyCNX shared 47.91%-70.16% identities with CNXs from other species. The mRNA transcripts of PyCNX were constitutively expressed in all the examined tissues, including gills, gonad, hepatopancreas, mantle and haemocytes, with the highest expression level in gills. The mRNA transcripts of PyCNX in gills were significantly up-regulated at 1 h (p < 0.01), down-regulated to similar level of Blank group at 3 h (p > 0.05) and 6 h (p > 0.05), and decreased significantly from 12 to 48 h (p < 0.05) after acute high temperature stress (25 °C). PyCNX was mainly located in the ER of haemocytes. The expression profiles of PyATF6, PyIRE1 and PyGRP78 in the gills of scallops after acute high temperature stress were investigated using quantitative real-time PCR. The mRNA transcripts of PyATF6 increased significantly at 1 h, 3 h and 6 h after acute high temperature stress (p < 0.01), then down-regulated to similar level of Blank group at 12 h (p > 0.05) and 24 h (p > 0.05), and finally significantly up-regulated again at 48 h (p < 0.05). Similar to PyATF6, the mRNA transcripts of PyIRE1 were significantly up-regulated at 1 h (p < 0.05), 3 h (p < 0.01), 6 h (p < 0.05) and 48 h (p < 0.05) after acute high temperature stress. The mRNA transcripts of PyGRP78 were significantly up-regulated at 3 h (p < 0.05), reached the highest level at 6 h (p < 0.01) after acute high temperature stress, and kept significant higher level at 12-48 h (p < 0.05). These results indicated that PyCNX was involved in the response upon the acute high temperature stress of scallops probably by regulating UPR.

The Price Difference and Trend Analysis of Yesso Scallop (Patinopecten yessoensis) in Changhai County, China

This paper focuses on the difference in inter-group and intra-group price of Yesso scallop (Patinopecten yessoensis) and the simulation accuracy of three different exponential smoothing models in the price. Based on the farm-gate price and wholesale price data of P. yessoensis in Changhai county from January 2017 to December 2018, this study uses the Wilcoxon rank sum test to compare the inter- and intra-group price and applies simple exponential smoothing (SES), Holt’s linear trend method, and Holt-Winters’ additive method to simulate and predict the price. The results suggest that (i) to improve economic benefits, it is necessary to formulate reasonable farming area and establish low-density ecological cultivation mode; (ii) the price’s Akaike information criterion (AIC) and mean absolute percentage error (MAPE) values by the SES model are optimal, and the MAPE value is lower than 4%; and (iii) the result of SES analysis shows no obvious change from January to March 2019.

Simultaneous determination of ten paralytic shellfish toxins and tetrodotoxin in scallop and short-necked clam by ion-pair solid-phase extraction and hydrophilic interaction chromatography with tandem mass spectrometry

Paralytic shellfish toxins and tetrodotoxin (puffer-fish toxin), the latter of which was recently found in bivalves from Europe, Japan, and New Zealand, are potent neurotoxins. A simple and effective clean-up procedure was developed for the simultaneous determination of ten paralytic shellfish toxins (gonyautoxins 1–6, decarbamoylgonyautoxins 2 and 3, and N-sulfocarbamoylgonyautoxins 2 and 3) and tetrodotoxin in the scallop, Mizuhopecten (Patinopecten) yessoensis, and the short-necked clam, Ruditapes philippinarum. To reduce matrix effects, 1% aqueous acetic acid extracts of the bivalves were cleaned up by ion-pair solid-phase extraction using a graphite carbon cartridge with tridecafluoroheptanoic acid as the volatile ion-pair reagent, followed by fourfold dilution. The ten paralytic shellfish toxins and tetrodotoxin were then separated on a hydrophilic interaction chromatography column and quantified by tandem mass spectrometry. The limits of detection and the limits of quantification for the ten PSTs ranged from 0.09 to 13.0 µg saxitoxin equivalents/kg and from 0.26 to 39.4 µg saxitoxin equivalents/kg, respectively. The limit of detection and the limit of quantification for tetrodotoxin ranged from 27.4 to 27.9 µg/kg and from 83.1 to 84.4 µg/kg, respectively. The proposed method yielded minimal matrix effects for the 11 analytes, thus allowing their quantification by simple external calibration. The proposed method also gave good mean recoveries of the 11 analytes ranging from 75.7 to 96.2% with relative standard deviations less than 16% at three fortification levels for the ten paralytic shellfish toxins (total concentrations of 277, 554, and 1107 µg saxitoxin equivalents/kg) and tetrodotoxin (100, 200, and 400 µg/kg) in the two bivalve samples. Finally, the proposed method was applied for the determination of the ten paralytic shellfish toxins and tetrodotoxin in scallop and short-necked clam samples.

Purification and structure analysis of zinc-binding protein from Mizuhopecten yessoensis.

Zn-binding protein was obtained after purification from scallops (Mizuhopecten yessoensis) using gel permeation and ion-exchange chromatography. Amino acid determination showed that the cysteine of the zinc-binding protein accounted for one-third of the total amino acids, which is a typical feature of metallothionein (MT). The spectra of Fourier Transform Infrared Spectroscopy (FTIR) and Circular Dichroism (CD) were analyzed to predict the secondary structure information of zinc-binding protein: the α-helix was 46.55%, the β-sheets was 27.07%, the random coil was 16.48%, and the β-turns was 9.89%. Using a commercial kit to measure its antioxidant activity in vitro, the result showed that it had good scavenging ability to 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH), and reducing the ability to ferrous iron ions. With the process provided by this study, zinc-binding protein can be prepared in large quantities, which is the basis for its future commercialization. PRACTICAL APPLICATIONS: According to the extraction and purification process established in this study, a large amount of zinc-bound MT from the viscera of scallops can be obtained. And the zinc-bound MT had good antioxidant activity. In addition, the yield of each purification step has been calculated. The zinc-bound MTs from scallop' viscera can be prepared in large quantities by directly using the process in this manuscript or by equal magnification of this process. In the future, large-scale production can be considered to increase the economic value of scallops' viscera.

A phosphorescence resonance energy transfer-based “off-on” long afterglow aptasensor for cadmium detection in food samples

Cadmium contamination is a severe food safety risk for human health. Herein, a long afterglow “off-on” phosphorescent aptasensor was developed based on phosphorescence resonance energy transfer (PRET) for the detection of Cd2+ in complex samples which minimizes the interference of background fluorescence. In this scheme, initially the phosphorescence of Cd2+-binding aptamer conjugated long afterglow nanoparticles (Zn2GeO4:Mn) was quenched by black hole quencher 1 (BHQ1) modified complementary DNA. Upon encountering of Cd2+, the aptamer interacted with Cd2+ and the complementary DNA with BHQ1 was released, leading to phosphorescence recovery. The content of Cd2+ could be quantified by the intensity of phosphorescence recovery with 100 μs gate time (which eliminated the sample autofluorescence) with a linear relationship between 0.5 and 50 μg L−1 and a limit of detection (LOD) of 0.35 μg L−1. This method was successfully demonstrated for Cd2+ detection in drinking water and yesso scallop samples. The “off-on” phosphorescent aptasensor based on PRET of long afterglow nanomaterials could be an effective tool for Cd2+ detection in food samples.

Synergistic gelation in the hybrid gel of scallop (Patinopecten yessoensis) male gonad hydrolysates and xanthan gum

This study evaluated the gel and microstructure properties of scallop (Patinopecten yessoensis) male gonads hydrolysates (SMGHs) combined with xanthan gum (XG). SMGHs/XG hydrogel matrix properties and structures were elucidated via different analysis tools such as rheometry, LF-NMR, FTIR, AFM, and Cryo-SEM. The addition of XG significantly improved the rheological properties of SMGHs, as indicated by 3.1-fold G' and 1.3-fold melting temperature with increasing the XG dose to 5.6mg/ml. The corresponding decrease in the T23 relaxation time from 450.3 to 365.6ms also signified the strong binding between SMGHs and XG. SMGHs/XG also had a higher proton density (T1 and T2 weighted images) due to the higher bound and free water content of the hybrid gel systems, respectively. Additionally, the blueshift in the amide I and II bands in SMGHs/XG further indicated stronger electrostatic interactions between SMGHs and XG. Such scenarios resulted in a well-distributed and compact network with a rougher surface of SMGHs/XG in comparison to pure SMGHs and XG, as assessed by AFM and SEM. These results suggest that SMGHs/XG gel could be a potential hybrid gel applied in the food industry. PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are edible, but are usually discarded during processing of scallop adductor. Because of its rich nutrition and gelation properties, scallop male gonads have a potential role in developing marine source-protein as a functional food base. The SMGHs/XG binary gel would be potentially applied in delivery system in food and biological fields. Further study is undergoing to apply SMGHs/XG binary gel to embed bioactive compounds, such as curcumin and β-carotene.

Effect of non-covalent binding of phenolic derivatives with scallop (Patinopecten yessoensis) gonad protein isolates on protein structure and in vitro digestion characteristics

The present study was aimed to investigate the effects of non-covalent interactions between scallop gonad protein isolates (SGPIs) and different concentrations (20, 120 and 240 μmol/g, protein basis) of four phenolic compounds, such as (-)-epigallocatechin gallate (EGCG), epicatechin 3-gallate (ECG), epigallocatechin (EGC), and catechin (C), regarding the structural and functional properties of the complex. Total sulfhydryl and surface hydrophobicity in SGPIs decreased by nearly 72% and 65% with 240 μmol/g EGCG, similar but less appreciable changes were produced by EGC, ECG and C. Fluorescence quenching and thermodynamic parameters suggested that hydrogen bond and van der Waals dominated the interaction process between SGPIs and EGCG, and the interaction was further studied by molecular docking. Moreover, EGCG-treated SGPIs digests exerted higher ABTS+• scavenging activity than that of ECG and EGC-treated SGPIs. These findings are helpful to reveal the binding mechanism of phenolics and SGPIs, and provide a theoretical basis for the application of SGPIs-phenolic complexes as functional food additives.

Gonadal somatic cell-specific transforming growth factor-β superfamily member in the Yesso scallop reveals gonadal somatic cell distribution during the reproductive phase.

The objective of this study was to identify the gonadal somatic cells in the Yesso scallop using a novel molecular marker. This study is the first to identify the bone morphogenetic protein 2a (Bmp2a) gene as a gonadal somatic cell-specific gene in this bivalve. We performed a transcriptomic survey to identify the transforming growth factor-β (TGFβ) superfamily members that act in Yesso scallop gonad development. BLAST survey, phylogenetic tree, and RT-PCR analyses screened BMP molecules (i.e., bmp2a and bmp10a), which are members of the TGFβ superfamily that show gonad-specific expression. Among the BMPs from the Yesso scallop, in situ hybridization accompanied by RNAscope assay identified that bmp2a mRNA was specifically expressed in the gonadal somatic cells localized in the interspace between germ cells. Real-time quantitative PCR (qPCR) analysis revealed that bmp2a mRNA expression increased during the reproductive phase. The relative expression of bmp2a mRNA was lowest at the beginning of the growing stage and peaked at the mature stage in both sexes. These observations indicate that bmp2a-positive gonadal somatic cells support germ cell growth and differentiation during the reproductive phase for both sexes. This study provides new insights into gonadal somatic cell biology in marine invertebrates and we propose that TGFβ signaling is necessary for gonad development in bivalves.

The involvement of PyBeclin 1 and PyLC3 in regulating the activation of autophagy in scallop Patinopecten yessoensis after acute high temperature stress

Beclin 1 and LC3 are important autophagy regulation proteins involved in vesicle nucleation and extension stage, respectively. In the present study, a Beclin 1 and a LC3 were identified from Yesso scallop Patinopecten yessoensis (PyBeclin 1 and PyLC3). The open reading frame (ORF) of PyBeclin 1 was of 1335 bp encoding a putative polypeptide of 444 amino acid residues with an N-terminal BCL-2 homology 3 (BH3) domain, a central coiled-coil domain (CCD), and a C-terminal evolutionarily conserved domain (ECD). The ORF of PyLC3 was of 369 bp encoding a putative polypeptide of 122 amino acid residues with an APG12 domain. The deduced amino acid sequences of PyBeclin 1 and PyLC3 shared 31.92–74.09% and 68.38–79.50% identities with Beclin 1s and LC3s from other species, respectively. The mRNA transcripts of PyBeclin 1 and PyLC3 were found to be expressed in all the examined tissues, including adductor muscle, gonad, gill, haemocytes and mantle, with the highest expression level in gill and haemocytes. The mRNA expression level of PyBeclin 1 in haemocytes increased significantly at 1, 3, 6, 12 and 24 h (2.98–4.07 fold of that in the Blank group, p < 0.05), and returned to normal level at 48 h after acute high temperature stress at 25 °C. Unlike PyBeclin 1, the mRNA transcripts of PyLC3 in haemocytes were significantly up-regulated at1, 3, 6 and 12 h (1.80–2.54 fold of that in the Blank group, p < 0.05), then decreased to blank level at 24 h (p > 0.05), and increased significantly again at 48 h (3.70 fold of that in the Blank group, p < 0.05) after high temperature. PyBeclin 1 and PyLC3 were mainly located in the cytoplasm and a small amount in the nucleus with few puncta, and the numbers of PyBeclin 1 and PyLC3 puncta increased at 3 h after acute high temperature stress. The LC3-II levels in gill and haemocytes were significantly up-regulated at 1 h and 3 h after acute high temperature stress. These results collectively suggested that PyBeclin 1 and PyLC3 were conserved members of Beclin 1 and LC3 family in scallops, and involved in regulating the activation of autophagy in scallops after acute high temperature stress.

Two new skeletal analogues of saxitoxin found in the scallop, Patinopecten yessoensis, as possible metabolites of paralytic shellfish toxins

The scallop, Patinopecten yessoensis, was screened for new saxitoxin analogues to study the metabolism of paralytic shellfish toxins (PSTs), and this resulted in the discovery of two new analogues: M5-hemiaminal (HA) and M6-HA. M5-HA was isolated and its structure was determined by using NMR spectroscopy. It contains hydrogen at C-4 with opposite stereochemistry to that in saxitoxin, and a hemiaminal was formed between 9-NH2 and the hydrated ketone at C-12 in α-orientation. This is the first reported structural feature in a natural saxitoxin analogue, whereas the same ring system has previously been reported in a synthetic saxitoxin analogue, FD-saxitoxin. Acid hydrolysis of the carbamoyl N-sulfate in M5-HA produced M6-HA which was also identified in P. yessoensis by using LC-MSMS. M5-HA was not synthetically produced from M1 (11-hydroxy gonyautoxin-5) and M3 (11,11-dihydroxy gonyautoxin-5) through incubation in aqueous buffers. Furthermore, PSTs in the hepatopancreas of P. yessoensis, cultured in a bay located in northeastern Japan, were chronologically analyzed in 2018. The highest concentrations of M1/M3/M5-HA were observed two weeks after C-toxins had reached their highest concentrations, which provides evidence that M1/M3/M5-HA are metabolites of C-toxins. The voltage-gated sodium channel blockage activity of M6-HA was not detected at the concentration of 140 nM by using the Neuro-2A veratridine/ouabain assay.

Francisellosis of Yesso scallops Mizuhopecten yessoensis in Japan is caused by a novel type of Francisella halioticida.

Francisella halioticida, the causative agent of francisellosis of the giant abalone Haliotis gigantea, has also been isolated from Yesso scallops Mizuhopecten yessoensis, which presented with orange/pinkish lesions in the adductor muscle and experienced high mortality. However, it is not clear whether the F. halioticida isolated from the giant abalone and Yesso scallops are phenotypically and genetically identical to each other. The present study revealed that isolates from the giant abalone and Yesso scallops were phenotypically different, with slower growth in modified eugon broth and a lack of prolyl aminopeptidase and phenylalanine aminopeptidase in Yesso scallop isolates. Additionally, we found that 3 of 8 housekeeping genes were different between them. Based on these phenotypic and genetic differences, we propose that F. halioticida isolated from Yesso scallops in Japan be designated as the 'J-scallop type' to distinguish it from strains from abalone ('abalone type'). Whole-genome sequencing analysis of a strain belonging to the J-scallop type showed that the overall similarity between the J-scallop and abalone type strains was estimated to be 99.84%. In accordance with a lack of prolyl aminopeptidase activity, in general, all of the J-scallop type strains examined have a 1 bp deletion in the responsible gene encoding prolyl aminopeptidase. This deletion was confirmed in all F. halioticida in diseased Yesso scallops examined, suggesting that in Japan, francisellosis of Yesso scallops is caused by a novel type of F. halioticida and not by the abalone type.

Prediction study on the spatiotemporal distribution of Yesso scallop larvae based on a coupled biophysical model

The Yesso scallop (Patinopecten yessoensis) larva takes more than 20 days from spawning to attachment. Due to natural environmental factors such as current and wind, the larvae are transported to a distance of more than hundreds of kilometers, leading to a high instability of their spatiotemporal distribution and great difficulties in their natural seedling collection. We built a Yesso scallop larvae's individual-based model (IBM hereon), combining the larvae's biological characteristics at five different stages in shape, survival rate, and specifications along with the temperature's effect. We built hydrodynamic and particle tracking models in the Northern Yellow Sea and Bohai Sea of China. By combining these models, we constructed a coupled biophysical spatiotemporal distribution model for the Yesso scallop larva. We verified the model with measured data, which showed a good agreement with the observed data. Then, we performed a numerical simulation to predict the Yesso scallop larvae's spatiotemporal distribution. We conclude the Lagrangian residual current in the domain caused by the tide's coaction, wind, and thermohaline effects are from northeast to southwest, which drives the Yesso scallop larvae from its spawning area to the natural seedling area. The Yesso scallop larvae's spatiotemporal distribution calculated with our model can guide natural seeding collection.

Cinnamaldehyde exerts prophylactic and therapeutic effects against Vibrio anguillarum infection in Yesso scallop (Patinopecten yessoensis) by its direct antimicrobial activity and positive effect on the innate immunity

This study investigated the antimicrobial mechanism and immunoprotection of cinnamaldehyde (CNA) against Yesso scallop (Patinopecten yessoensis) pathogen Vibrio anguillarum (V. anguillarum). The results showed that V. anguillarum cells treated with CNA displayed an increased uptake of 1-N-phenylnaphthylamine and propidium iodide, as well as distinct morphological changes, which was indicative of its potent antibacterial activity against V. anguillarum via a membrane-active mechanism to kill the bacteria. Biosafety analysis showed that CNA exhibited no adverse effects on the hemocytes under the test concentrations. Most importantly, pre-treating, co-treating, and post-treating the scallop with CNA significantly increased the survival rate of the scallop after the injection of V. anguillarum. In vivo functional analysis further demonstrated that CNA could promote the clearance of bacteria in hemolymph. Further examination revealed that CNA had a significantly positive effect on the hemolymph immune parameters, contributing to the scallop's immune defense against pathogenic infections. In addition, CNA modulated the expressions of immune-associated genes like PGRP, Toll, NFκB, Defensin, Lysozyme, GPx, and Cu/Zn SOD as analyzed by a Real-Time PCR approach. Furthermore, it is interesting to note that pre-treatment with CNA mitigated a series of V. anguillarum-induced genes known to be responsible for antibacterial and anti-oxidant reactions. These results suggested that CNA exhibited potential for development as a new antibacterial strategy to combat scallop bacterial infections in aquaculture.

Structure-dependent absorption of atypical sphingoid long-chain bases from digestive tract into lymph

BackgroundDietary sphingolipids have various biofunctions, including skin barrier improvement and anti-inflammatory and anti-carcinoma properties. Long-chain bases (LCBs), the essential backbones of sphingolipids, are expected to be important for these bioactivities, and they vary structurally between species. Given these findings, however, the absorption dynamics of each LCB remain unclear.MethodsIn this study, five structurally different LCBs were prepared from glucosylceramides (GlcCers) with LCB 18:2(4E,8Z);2OH and LCB 18:2(4E,8E);2OH moieties derived from konjac tuber (Amorphophallus konjac), from GlcCers with an LCB 18(9Me):2(4E,8E);2OH moiety derived from Tamogi mushroom (Pleurotus cornucopiae var. citrinopileatus), and from ceramide 2-aminoethyphosphonate with LCB 18:3(4E,8E,10E);2OH moiety and LCB 18(9Me):3(4E,8E,10E);2OH moiety derived from giant scallop (Mizuhopecten yessoensis), and their absorption percentages and metabolite levels were analyzed using a lymph-duct-cannulated rat model via liquid chromatography tandem mass spectrometry (LC/MS/MS) with a multistage fragmentation method.ResultsThe five orally administered LCBs were absorbed and detected in chyle (lipid-containing lymph) as LCBs and several metabolites including ceramides, hexosylceramides, and sphingomyelins. The absorption percentages of LCBs were 0.10–1.17%, depending on their structure. The absorption percentage of LCB 18:2(4E,8Z);2OH was the highest (1.17%), whereas that of LCB 18:3(4E,8E,10E);2OH was the lowest (0.10%). The amount of sphingomyelin with an LCB 18:2(4E,8Z);2OH moiety in chyle was particularly higher than sphingomyelins with other LCB moieties.ConclusionsStructural differences among LCBs, particularly geometric isomerism at the C8–C9 position, significantly affected the absorption percentages and ratio of metabolites. This is the first report to elucidate that the absorption and metabolism of sphingolipids are dependent on their LCB structure. These results could be used to develop functional foods that are more readily absorbed.

참가리비 기름담금통조림의 제조 및 품질특성

참가리비 기름담금통조림의 제조 및 품질특성

The role of the cadmium-binding protein response of the digestive gland of the Yesso scallop Mizuhopecten yessoensis (Jay, 1857) for marine environmental assessments

The ability of Pectinidae to accumulate heavy metals and store them in their tissues allows the use of scallops for biomonitoring marine pollution. High molecular weight metallothionein (MT)-like proteins (MTlps) play a central role in this process. Two major MTlps (72 and 43 kDa) have been identified in the digestive glands of Mizuhopecten yessoensis (Yesso scallop). These proteins have a very high affinity for the heavy metals cadmium, cobalt, and caesium. Additionally, these proteins can be deposited in large quantities in the digestive glands of this mollusc. It has been shown that 72 kDa MTlp is the main stress-response protein in areas polluted with cadmium or radioactive metals. Monitoring the amounts of MTlps in the digestive glands of the scallop M. yessoensis in areas with different anthropogenic pollutants has shown that these proteins are reliable biological markers of heavy-metal pollution in the marine environment.

Intermolecular interaction in the hybrid gel of scallop (Patinopecten yessoensis) male gonad hydrolysates and κ-carrageenan.

Various bond disrupting agents including NaCl, GuHCl, urea, and SDS were introduced to investigate the intermolecular interactions between scallop (Patinopecten yessoensis) male gonad hydrolysates (SMGHs) and κ-carrageenan (κ-C), which were monitored by changes in rheological property, water distribution, conformation characterization and microstructure by using rheometer, low field-NMR relaxometry, Fourier transform infrared (FTIR) spectroscopy, cryo-scanning electron microscopy (cryo-SEM), and confocal laser scanning microscopy. The results showed that the bond disrupting agents deteriorated the rheological property of SMGHs/κ-C in a dose-dependent manner. Indeed, at the same concentration of 2 M, NaCl deteriorated the SMGHs/κ-C more obviously than GuHCl and urea. In addition, SMGHs/κ-C with bond disrupting agents possessed higher relaxation times including T21 and T23 , indicating the migration to free water direction of bound and free water. Moreover, the FITR results showed the red-shift in water regions (amide A and B bands), amide I and II bands, and indicated the breakdown of hydrogen bonds and electrostatic interactions, indicating a disordered structure in SMGHs/κ-C by various bond disrupting agents. Furthermore, cryo-SEM results showed the change of SMGHs/κ-C from a homogeneous network to a looser and ruptured one with larger void spaces, and indicated the disrupted and tattered microstructure of SMGHs/κ-C by various bond disrupting agents. Additionally, SMGHs/κ-C as well showed less aggregates stained by RITC by bond disrupting agents. These results suggest that electrostatic interactions would be mainly involved in the maintenance of SMGHs/κ-C gel network. This study could provide theoretical and methodological basis for hydrogel products with modified gel strength and microstructure by understanding the intermolecular interactions in gel system. PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads as a high-protein part of scallop, is usually discarded during processing despite its edibility. In recent years, scallop male gonads are regarded as good sources to develop protein matrices due to their high protein content and numerous nutrients. In this study, scallop male gonad hydrolysates (SMGHs) were obtained by trypsin-treated process. The considerable gelation behavior of SMGHs indicated that the SMGHs could be potentially utilized as a novel thickener and additive in production of kamaboko gels, can, sausage and spread with marine flavor.