In this paper, a new dual-pocket (DP), dielectric modulated (DM) heterostructured tunnel field-effect transistor (DM-HTFET)-based biosensor has been reported. First, the efficacy of the DP-hetero-TFET (HTFET) has been analyzed by comparing it with other existing TFET structures. Next, a comprehensive assessment of sensitivity between single-pocket (SP) and DP-DM-HTFET biosensors for pocket thickness ( ${T}_{\text {pocket}}$ ), pocket length ( ${L}_{\text {pocket}}$ ), pocket doping ( ${N}_{\text {pocket}}$ ), work function of the gate metal, molar fraction of Ge, gate oxide layer, and gate oxide layer thickness ( ${T}_{\text {ox}}$ ) was done. Hence, a nonuniform arrangement of biomolecules inside the cavity simulation has been done using ATLAS device simulation software to validate the working ability of the proposed sensor. Significant improvement in the sensitivity due to threshold voltage (ON-current) i.e., 26.78% (78.5%), 60.8% (40.4%), 56% ( ${2.2} \times {10}^{{2}}$ %), and 40.6% (80.68%) has been observed for the DP-DM-HTFET over SP with the variation of ${T}_{\text {pocket}}$ , ${L}_{\text {pocket}}$ , ${N}_{\text {pocket}}$ , and ${T}_{\text {ox}}$ , respectively. DP-DM-HTFET-based current mirror circuit has also been demonstrated at the end. Sensitivity evaluation discloses that the DP-DM-HTFET can be a promising candidate for CMOS-based label-free biosensing applications.