AYUSH Research Articles

A study on awareness and attitude of youth towards the use of herbal cosmetic products in Rajkot district

There is a significance growth in allocation of funds in budget 2022-23 by government of India to Ministry of Ayush as compare to last 10years. At the same time data researched and revealed by ministry and CII shows that CAGR is increasing rapidly of herbal products. Rajkot district is populated and multi cultured city. Youth in particularly will be the contributor to this herbal cosmetic industry in future. Therefore this research mainly focused on awareness and attitude towards herbal cosmetic product amongst youth between 15-29 years of age. Study also focuses their difference of opinions based on gender and age wise.Total Revenue in the Herbal Cosmetics segment amounts to US$0.84bn in 2022. The market is expected to grow annually by 3.41% (CAGR 2022-2026). This shows the potential for growth but at the same time it is important to see awareness and attitude of youth in Rajkot district so that government and companies can design their strategies accordingly.In this research, after studying a lot of literature, data released by government official and research output of CII, researcher has decided to use descriptive research methodology. Sampling technique used is Convenience (non-random sampling) sampling with sample size of 237 out of total population of Rajkot district within decided sample frame.This study reveals that awareness amongst youth related to herbal cosmetic product is very high but at the same time only 9.3% respondents using herbal cosmetics products frequently in their day to day life. If respondents are given a chance they majority of respondents are willing to use herbal cosmetic products. This shows a huge potential to grow in this segment in near future.

Research output from India's Siddha system of medicine during 1972-2019: Bibliometric analysis points need to focus on quantity and quality.

The Siddha system of medicine (SSM) is one of the Indian Systems of Medicine (ISM). In recent times, it has received adequate support from the Indian Ministry of Ayush. Like many such indigenous systems of medicine globally, the SSM is based on its own theoretical principles, philosophy, and methods of practice. Nevertheless, SSM conducts and needs basic and applied research using scientific methods. Such research is evidenced by research publications. In the absence of a review, we did a bibliometric analysis of research publications from SSM for the period of 1972-2019. We used the term "Siddha" to retrieve the data from the PubMed database. We retrieved data from the National AYUSH research portal and homepages of institutions mandated to do research in SSM. We abstracted bibliometric information and used Epi info (Version 7.2) for analysis. We described the trends and key characteristics. Of a total of 2009 retrieved articles, we included 1457 (73%) for analysis. Between 1972 and 2001, the median number of publications per year was three (range: 0-13). Beyond 2012, the publications increased exponentially with an annual growth of 23%. The maximum number of publications (n = 224) was in 2019. Among 43 countries that produced the papers, India topped it (88%). Of 1457, 1.7% (n = 25) of publications from Siddha institutions were indexed in PubMed. A bibliometric review of research output from SSM suggests that the publications have increased in the last two decades. The review recommends improving research output from SSM in terms of quantity and quality.

Homoeopathy of AYUSH in Alopecia Areata

The article deals with a trichological problem & sees the role of homoeopathy of the AYUSH ministry in dealing with Alopecia Aerata (AA). It begins with the description of the condition followed by its epidemiology at the global & national level. The literature review deals with the description of the problem across the various regions of the globe & how studies have suggested evolving ways to deal with the issue. Various sections like clinical features, risk factor, causes, treatment, support system that is required are the highlights of the article. The burden, challenges are mentioned followed by a section that describes the ‘to do’ activities in future. A homoeopathic treatment protocol is suggested at the end of the article while further aspiring that the integration is done at the programmatic level for the benefit of the masses.

Current Status of Rule 170 in Drugs and Cosmetics Act & Rules, 1940 & 1945

Drugs and Cosmetics Act, 1940 (Act 23 of 1940) is a pre-independence Act passed by British Legislature under the Government of India Act, 1935 as the Drugs Act, 1940. The main objective of this act is to regulate the import, manufacture, distribution and sale of Ayurveda, Siddha, Unani drugs. The Drugs and Cosmetic Rule came in existence as a part of force vide Notification No.F.28-10/45 (H) (1), the 21st December, 1945, Department of Health, Government of India, New Delhi. Drugs and Cosmetic Rules, 1945 composed of 19 parts containing 170 rules. The rules 151 to 170 are pertaining to Ayurveda, Siddha, Unani drugs. The review aims to get a transparency regarding the current status of Rule 170 of Drugs and Cosmetic Rule, 1945, as this rule is in a state of uncertainty. Rule 170 and its provisions 0f Drugs and Cosmetic Rules, 1945 mainly deals with the prohibition of misleading advertisement of Ayurveda, Siddha or Unani drugs. On 29th August 2023 the notification from Ministry of Ayush in relation to the omission of Rule 170, creates dilemma in public. In that notification it is clearly stated that, With the power confers under section 33P of Drugs and Cosmetic Act, 1940, all States/UTs licensing authorities are here by directed not to initiate or take action under the Rule of 170 of Drugs and Cosmetic Rules, 1945. Together with this notice it also mentioned that the notification for omissions of Rule 170 and its related provisions mentioned in Drugs and Cosmetic Rules, 1945 will take some time. The letter was issued based on a recommendation from the ASUDTAB and developed based on the existing case regarding the Patanjali products. A part from this in “THE INDIAN GAZETTE” of 2nd February 2024, Ministry of AYUSH notified the draft of certain rules further to amend the Drugs Rules, 1945. In that over the serial number 33 mentions regarding the omission of Rule 170, it is quoted like “The Rule 170 of the principal rules, shall be omitted”. As per the objection/opinion received from the public over the drafted rules published by Ministry of Ayush in relation to Drugs and Cosmetic Rules, 1945, a latest notification of “THE INDIAN GAZETTE” (July 2nd of 2024) also suggesting that “Rule 170 shall be omitted”. By reviewing this, it is found that still the Rule 170 is not get omitted from the Drugs and Cosmetic Rules, 1945, instead of omission it got neutralised based on the section 33P of Drugs and Cosmetic Act and it is on consideration for omission.

When regulation ignores evidence: The case of Denmark’s Ashwagandha ban and the Technical University of Denmark’s misguided risk assessment

The recent ban on Ashwagandha (Withania somnifera (L.) Dunal) supplements in Denmark, based on a risk assessment by the Technical University of Denmark (DTU), exemplifies the challenges in regulating traditional herbal medicines within modern frameworks. This article critically examines the DTU report, highlighting methodological flaws including overreliance on animal studies and selective literature review. The report’s disregard for Ashwagandha's long history of traditional use and mischaracterization of its effects, particularly alleged abortifacient properties, contradicts a growing body of scientific evidence supporting its safety and efficacy. The current research demonstrating Ashwagandha's benefits in stress reduction, sleep improvement, and cognitive function further underscores the disparity between scientific findings and the DTU’s conclusions. The ban’s implications extend beyond Denmark, raising questions about regulatory consistency and proportionality in evaluating herbal products globally. The Indian Ministry of Ayush’s critique emphasizes the need for a more comprehensive, evidence-based approach for assessing traditional medicines. This controversy may catalyze the development of integrated evaluation methods, such as the Collaborative Medicine and Science framework, bridging diverse knowledge systems in regulatory decision-making. The case underscores the necessity for a global health paradigm that harmonizes traditional and modern medical approaches, ensuring public safety while preserving access to beneficial traditional remedies.

Fostering Health and Heritage: Ministry of Ayush’s Aushadha evam Poshana Vatika Initiative in Ekalavya Model Residential Schools (EMRS)

Fostering Health and Heritage: Ministry of Ayush’s Aushadha evam Poshana Vatika Initiative in Ekalavya Model Residential Schools (EMRS)

Contributions of the National Medicinal Plants Board (NMPB), Ministry of Ayush in the medicinal plants sector - An appraisal

The Government of India set up the National Medicinal Plants Board (NMPB) to promote the medicinal plants sector on November 24, 2000, under the Chairmanship of the Union Minister of Health and Family Welfare. The board is a nonstatuary coordinating body and is now a section of the Ministry of Ayush (MoA). The primary mandate of NMPB is to develop an appropriate mechanism for coordination between various ministries/departments/organizations and implementation of support policies/programs for overall (conservation, cultivation, trade, and export) growth of the medicinal plants sector both at the central/state and international level. NMPB, under its Central Sector Scheme (CSS) on “Conservation, Development and Sustainable Management of Medicinal Plants,” provides project-based financial support to explore various aspects of medicinal plants. Different scheme components of NMPB are functioning, such as Conservation, Research and Development (R&D), Information, Education, and Communication and Training, Herbal Gardens, Marketing and Trade, and International Cooperation. NMPB implements various programs/policies through its 37 State Medicinal Plants Boards at different state/UT levels, 6 Regional Cum Facilitation Centers at the region level, and also through various State Forest Departments/Horticulture Departments/Agriculture Departments. NMPB is also assisted by the Project Screening Committees, Project Approval Committee, and other Committees constituted as per the requirement of the CSS guideline. The present article deals with the overall contribution of NMPB to the medicinal plant sectors since its inception. The article places interest in outcomes that would gradually help the country meet its international obligation in various aspects of medicinal plants/sectors.

Development of “eIndianMedicine” App for subclinical infection of COVID-19

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease (COVID-19) pandemic and has infected more than 561M people worldwide. The World Health Organization (WHO) has declared it an international public health concern. Approximately 80% of subclinical COVID-19 cases continue to rapidly spread in India. Consequently, the Indian Government urges the scientific community to control its transmission by developing self-assessed mobile apps and searching for Indian systems of medicine. The Ministry of Ayush, Government of India, recently recommended Ayurvedic medicine for the treatment of COVID-19 subclinical infection. However, awareness of Indian systems of medicine is not yet popularized because of low consumer perception and ignorance of scientific facts in India. This study provides a perspective on the design and development of a mobile app “eIndianMedicine for COVID-19” with a self-assessment tool. The abstraction of scientific facts regarding Indian systems of medicine is important for the development of such information tools in India. It can gather information from any patient to stop the spread of a silent carrier and to start treatment immediately. This can reduce mass testing costs and awareness of the Indian systems of medicine. Hence, current progress in information technology can increase the mobilization of International Trade in Indian traditional medicine to treat asymptomatic COVID-19 patients worldwide.

UFLC-MS Method Development of Vasicinone, Pellitorine, 6-Gingerol, Costunolide, Dehydrocostuslactone, Apigenin, and Validation of Piperine, Biflorin in Polyherbal Formulations

Standardisation of polyherbal formulations is necessary for the quality, safety, quantity, and efficacy of botanicals in marketed and newly established formulations. The Ministry of Ayush, Govt of India, recommended using polyherbal formulations to treat COVID-19, i.e., Kabusura kudineer, Nilavembu kudineer, etc., Kabusura kudineer is a Siddha-based formulation. It prevents and treats COVID-19 due to some botanicals in Kabusura kudineer, which have proven anti-inflammatory, anti-viral, and immunomodulatory effects. The current work focuses on establishing a standard protocol for the Kabusura kudineer marketed, a novel dosage form called Hydaljss08, and in plant species, mainly present in both formulations. Both formulations contain some similar crude drugs and their active constituents. They are Zingiber officinale rhizome, Syzygium aromaticum flower buds, Adhatoda vasica leaves, Anacyclus pyrethrum roots, Saussurea lappa roots, Piper longum fruits, Clerodendrum serratum roots, Coleus amboinicus roots, contain active phytopharmaceuticals are 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin. Existing liquid chromatography methods were reported for individual above active compounds, but not in these formulations and combined dosage forms. Working UFLC methods have not been reported individually nor combined for the Biflorin. The current study aims to develop UFLC methods for 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin in polyherbal formulations Kabusura kudineer marketed, Hydaljss08 and in isolated, fractions, extract of plant species present in both dosage forms. The preliminary identification of the phytopharmaceuticals in the polyherbal formulations, isolated fractions, and extract of plant species was done by TLC and IR spectrum. The developed liquid chromatography method was novel, simple, linear, and rapid for estimating 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, apigenin in a plant species, and Ayush-based formulations.

Ayush Kwath: A Major Contribution of Ayurveda in Preventing COVID- 19 Infection

Background: Ayush Kwath is an Ayurvedic polyherbal formulation (PHF) of dry Tulsi leaves, Cinnamon bark, Sunthi rhizome, and black pepper fruit. Methods: This PHF contain phytochemicals that help in boosting immunity; hence, it is suggested as a preventive measure against the coronavirus. Ayurveda is an ancient Indian medicine system that offers treatment for various diseases with the help of herbal, animal, or mineral-based formulations. Currently, the entire globe is facing the challenge of combating the COVID-19 pandemic. COVID-19 (Coronavirus disease 19) is a highly infectious disease caused by the SARS-CoV-2 virus (Severe Acute Respiratory Syndrome Coronavirus-2) that belongs to a large family of coronaviruses. Results: Most people who are affected by this virus experience mild to moderate symptoms of pneumonia, but older people or those with weakened immune systems, for example, those suffering from cancer, diabetes, cardiovascular disease, or chronic respiratory diseases, are more likely to develop serious respiratory illnesses. As there is no registered medicine against COVID-19, strengthening the immune system is an effective way to tackle this disease. Conclusion: Ayush Kwath, a natural immunity booster, has been approved by the Ministry of AYUSH as a prophylactic measure to fight against novel coronavirus infection.

‘REVIEW OF MEDICINAL PLANTS USED IN VETERINARY PRACTICES’ - A BOOK REVIEW

India, with its divine diversity, is one of the wealthiest nations with flora, fauna, and herbal medicinal wealth. Ayurveda, the oldest existing medical science of India, with a vast type of remedies, has maintained the health of individuals and cured diseases since time immemorial. It is not limited to humans but extended to animals (Pashu-Ayurveda) and trees (Vriksha-Ayurveda). Animals and animal products play a significant role in the country's economy. The Indian government has allotted a big budget for various projects for the maintenance of the health of animals and to gain high-quality by-products as well. Looking at this scenario, the current article is a review of a book entitled, ‘Review of Medicinal Plants Used in Veterinary Practices', published by the Central Council for Research in Ayurvedic Sciences (C.C.R.A.S.), Ministry of AYUSH, Government of India, New Delhi.

Homoeopathy & the ‘Sunshine Vitamin’

There are four fat soluble vitamins & two water soluble vitamins. The fat soluble vitamins are A, D, E & K. The water soluble ones are B & C. The current article discusses the fat soluble vitamin D & its relation to homoeopathic therapeutics. Among the AYUSH ministry of the Government of India, Homoeopathy is ‘H’ in the AYUSH acronym. In homoeopathic therapeutics, oils are a source of medicine. There are many medicines in homoeopathy that are prepared from oils. The sunshine vitamin medicines are such medicine prepared from oils. The role of these medicines is seen in the light of the role of the vitamin D in the body. The benefits of homoeopathy through its qualities of cost effectiveness, clinical effectiveness & zero side effects can be used for mass coverage. Similarly, masses can benefit through large scale roll out of the medicine prepared from the oil which is the source of vitamin D. The dual benefit of this homoeopathic medicine is that it can be used as a supplement & medicine as well. Therefore, this medicine can be a tool in the vitamin A prophylaxis program, as a supplement in the nutritional programs & finally in bone health through its role in calcium & phosphorus metabolism in the body.

Homoeopathy in Cervical Cancer

Population growth is related to high fertility rates, thus grading multiparous women in the risk zone of cervical cancer.Cervical cancer is a chronic phenomenon of cervical erosion. Following that the body goes through is the Human Papilloma Virusinfection, which becomes the main reason for cervical cancer. The review article deals with the status of cervical cancer at theglobal level, followed by the situation in India. Further, the article discusses the role and status of HPV vaccines globally andnationally. The role of homeopathy of the Ministry of AYUSH of India in cervical cancer is discussed in the later section of thearticle. Here, it is proposed that a multi-stage treatment protocol based on therapeutics in homoeopathic materia medica will bean effective tool. The article's focus on homeopathy as the therapeutic system which can cover masses through its properties likecost effectiveness, therapeutic effectiveness, and less side effects.

Homoeopathy in the Eyes of Legislation in India

Therapeutics systems are governed by public health laws of the nations in which these are rolled out & practiced. The current article discusses the therapeutic system of Homoeopathy of AYUSH in India & the public health laws that govern this therapeutic system in India. The next step which the article goes through is the journey of the therapeutic system in the nation which becomes the pivotal for the article. The current article deals with the current situation of the homoeopathic therapeutic system at global followed by the national level. As an intervention strategy to deal with the roll out modalities of homoeopathy, the article discusses the journey of its related public health laws also. Finally, the article discusses the role of homoeopathy of AYUSH ministry of India in the current context & proposes a multi stage involvement based on the successful therapeutics in Homoeopathic Materia Medica. The focus of the article on homoeopathy & its related public health laws is critical as it can cover masses as it is cost effective, therapeutically effective & has no side effects. In order to achieve the roll out of these beneficial properties, the related public health laws need effective implementation.

In silico network pharmacology study on Glycyrrhiza glabra: Analyzing the immune-boosting phytochemical properties of Siddha medicinal plant against COVID-19.

Immunosenescence is a pertinent factor in the mortality rate caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The changes in the immune system are strongly associated with age and provoke the deterioration of the individual's health. Traditional medical practices in ancient India effectively deal with COVID-19 by boosting natural immunity through medicinal plants. The anti-inflammatory and antiviral properties of Glycyrrhiza glabra are potent in fighting against COVID-19 and promote immunity boost against the severity of the infection. Athimadhura Chooranam, a polyherbal formulation containing Glycyrrhiza glabra as the main ingredient, is recommended as an antiviral Siddha herb by the Ministry of AYUSH. This paper is intended to identify the phytoconstituents of Glycyrrhiza glabra that are actively involved in preventing individuals from COVID-19 transmission. The modulated pathways, enrichment study, and drug-likeness are calculated from the target proteins of the phytoconstituents at the pharmacological activity (Pa) of more than 0.7. Liquiritigenin and Isoliquiritin, the natural compounds in Glycyrrhiza glabra, belong to the flavonoid class and exhibit ameliorative effects against COVID-19. The latter compound displays a higher protein interaction to a maximum of six, out of which HMOX1, PLAU, and PGR are top-hub genes. ADMET screening further confirms the significance of the abovementioned components containing better drug-likeness. The molecular docking and molecular dynamics method identified liquiritigenin as a possible lead molecule capable of inhibiting the activity of the major protease protein of SARS-CoV-2. The findings emphasize the importance of in silico network pharmacological assessments in delivering cost-effective, time-bound clinical drugs.

Evaluating therapeutic potential of AYUSH-64 constituents against omicron variant of SARS-CoV-2 using ensemble docking and simulations

The COVID-19 pandemic in the later phase showed the presence of the B.1.1.529 variant of the SARS-CoV-2 designated as Omicron. AYUSH-64 a poly herbal drug developed by Central Council for Research in Ayurvedic Sciences (CCRAS) has been recommended by Ministry of Ayush in asymptomatic, mild to moderate COVID-19 patients. One of the earlier, in-silico study has shown the binding of the constituents of AYUSH-64 to the main protease (Mpro) of the SARS-CoV-2. This study enlisted four phytochemicals of AYUSH-64, which were found to have significant binding with the Mpro. In continuation to the same, the current study proposes to understand the binding of these four phytochemicals to main protease (Mpro) and receptor binding domain (RBD) of spike protein of the Omicron variant. An enhanced molecular docking methodology, namely, ensemble docking has been used to find the most efficiently binding phytochemical. Using molecular dynamics (MD) simulations and clustering approach it was observed that the Mpro and RBD Spike of Omicron variant of SARS-CoV-2 in complex with human ACE2 tends to attain 4 and 8 conformational respectively. Based on the docking studies, the best binding phytochemical of the AYUSH-64, akummicine N-oxide was selected for MD simulations. MD simulations of akummicine N-oxide bound to omicron variant of Mpro and RBD Spike-ACE complex was performed. The conformational, interaction and binding energy analysis suggested that the akummicine N-oxide binds well with Mpro and RBD Spike-ACE2 complex. The interaction between RBD Spike and ACE2 was observed to weaken in the presence of akummicine N-oxide. Hence, it can be inferred that, these phytochemicals from AYUSH-64 formulation may have the potential to act against the Omicron variant of SARS-CoV-2.

ADRs (Adverse Drug Reactions) related to ayurvedic medicine used in COVID-19

Background: Adverse Drug Reaction (ADR) is a response to a drug which is noxious and unintended, and which occurs at therapeutic doses. Due to a lack of systematic documentation regarding the occurrence of ADR and other issues to the safety of Ayurvedic medicines, concern about the traditional medical system has recently been increased. This is seen by a rise in safety reports and misunderstandings, especially in the recent pandemic.
 Objective: To monitor and analyse the suspected ADR to Ayurvedic medicines used in COVID-19.
 Materials and Methods: ADR monitoring data was collected through spontaneous and intensive method for a span of 2-year (April 2020 to April 2022) during the peak days of COVID-19 from the IPvCC of the National Institute of Ayurveda, Jaipur, India. The IPvCC Program is governed by NPvCC under the supervision of Ministry of AYUSH, Govt. of India, New Delhi. NPvCC is ultimately responsible to monitor and recommend regulatory interventions on the generated national ADR data.
 Result: In a span of two-year, 142 adverse drug events were reported out of which 17 were confirmed as ADR due to suspected Ayurvedic drugs.
 Conclusion: Overall review demonstrated that Ayurveda has traditionally been placed a strong emphasis on treatments that aim to heal the underlying illness while preventing the development of new illnesses.
 Bangladesh Journal of Medical Science Vol. 23 No. 01 January’24 Page : 237-245

JIVANEEYAM – Workshop on collection of basic information for the preparation of integrative management protocol for cancer

Abstract Jivaneeyam 2020, a 2-day workshop, was organized on February 10 and 11, 2020, by the Center for Integrative Oncology, All India Institute of Ayurveda, New Delhi. The workshop aimed to collect basic information and prepare an integrative management protocol for cancer. The event was sponsored by the Information, Education, and Communication Department of the Ministry of Ayush, Government of India, New Delhi. The main objective of the workshop was to facilitate a dialog between experts from modern and Ayurvedic medicine on the collection of basic information and treatment protocols for various types of cancer. The results of this dialog will help to prepare an integrative protocol for different cancerous conditions and ultimately reduce the global burden of the disease.

STANDARDIZATION OF PANCHKOLA CHURNA – A PHARMACEUTICAL AND CHEMICAL STUDY

In Ayurvedic classics, many categories of formulations are described, among which Churna is one. Churnas with single or multiple plant-origin drugs as ingredients are narrated in Ayurvedic texts for curing many diseases. The present research work deals with the preparation of Panchkola churna and its analysis for establishing the Pharmacopoeial standard as since now, no tests and measures are being worked out for assuring its quality parameters by the existing Ayurvedic Pharmacopoeial Commission Govt. of India, Ministry of AYUSH. The two batches of this particular Churna are made following the Standard operative process (SOP). The physio-chemical standards of these two batches PC-I & PC-II, were worked out along with TLC & HPTLC. The LOD, Ash Acid Insoluble Ash, Alcohol & water-soluble extractive, and a pH of 10% aqueous solution of the drug were worked out, and a range was established for giving the standards. The shelf-life period was also studied for the above samples concerning the tests and standards compared at 0, 6 and 12-month intervals. It was found that the values of most of the physio-chemical standards remain more or less the same. Still, a massive change in alcohol & water-soluble extractives after 12 months was observed, which signifies that under ordinary packing conditions, it can be kept for 12 months without losing its efficacy. The HPTLC standards at 200nm, 350nm, and 400nm of successive Rf are also worked out for knowing the standards.

Novel “HYDALJSS08” Polyherbal Formulation Development and Ultra-Performance Liquid Chromatographic Separation, Estimation of Berberine in Cissampelos pareira Roots, Tinospora cordifolia Stems, and A Marketed Siddha-Based Polyherbal Formulation “Kabusura Kudineer.”

The present study aims to develop and document an analytical method for Berberine in the chloroform fraction of Cissampelos pareira (Menispermaceae)roots, Tinospora cordifolia (Menispermaceae) stems, and Polyherbal formulations (Marketed & Developed). During the pandemic Covid-19 ministry of Ayush, Govt of India was recommended a Siddha-based polyherbal formulation known as “Kabusura Kudineer” for immune booster and treatment of Covid -19. False pareira roots having active biomarker substances are BBE proved for its activity of Anti-viral, and immunomodulatory. Preliminary identification of Berberine in chloroform fraction of Cissampelos pareira roots, Tinospora cordifolia stems was done by the TLC, mobile phase used as a Toluene: Acetone: Water (5:15:1) and Chloroform: methanol (70:20). The UPLC was performed on a column, X Bridge BEH C18 Column, 130Å, 3.5µm, 4.6mm X 150mm, 1/pk and Solvent system were 0.1% Trifluoroacetic acid: Methanol (60:40). The effluents were observed at 272nm in UV detector. The Rf value of Berberine, Chloroform fraction of Cissampelos pareira roots was 0.23, and the Tinospora cordifolia stems was 0.63. The valid UPLC method parameter for BBE is system precision, SD (5433.07), the Regression equation was found y = 20570 x−182430, Correlation coefficient (R2) 0.9993. The adequate Linearity concentration was found to be 50 to 150µg/ml, LODs (4.02µg/ml), LOQs (12.17µg/ml), Method precision for intraday, Interday %RSD (0.7), (1.4), SD (16064.5), (32811.5), Recovery 98%, and 102%. BBE content was found in a formulation (“KabusuraKudineer” churna- 20.9360µg/ml, Developed Hydroalcoholic Polyherbal formulation - 21.4370µg/ml), and the Chloroform fraction of Cissampelos Pareira roots (CHFCP), Chloroform fraction of Tinospora cordifolia stems (CHFTC) was 28.9090µg/ml and 73.2050µg/ml. The developed liquid chromatography (UPLC) methods have enabled simple, rapid, easy, accurate, reproducible, and linear analysis of BBE in Chloroform fraction of velvet roots, Tinospora cordifolia stems, and Ayurvedic, Siddha -based Polyherbal formulations.