Novel “HYDALJSS08” Polyherbal Formulation Development and Ultra-Performance Liquid Chromatographic Separation, Estimation of Berberine in Cissampelos pareira Roots, Tinospora cordifolia Stems, and A Marketed Siddha-Based Polyherbal Formulation “Kabusura Kudineer.”

Abstract

The present study aims to develop and document an analytical method for Berberine in the chloroform fraction of Cissampelos pareira (Menispermaceae)roots, Tinospora cordifolia (Menispermaceae) stems, and Polyherbal formulations (Marketed & Developed). During the pandemic Covid-19 ministry of Ayush, Govt of India was recommended a Siddha-based polyherbal formulation known as “Kabusura Kudineer” for immune booster and treatment of Covid -19. False pareira roots having active biomarker substances are BBE proved for its activity of Anti-viral, and immunomodulatory. Preliminary identification of Berberine in chloroform fraction of Cissampelos pareira roots, Tinospora cordifolia stems was done by the TLC, mobile phase used as a Toluene: Acetone: Water (5:15:1) and Chloroform: methanol (70:20). The UPLC was performed on a column, X Bridge BEH C18 Column, 130Å, 3.5µm, 4.6mm X 150mm, 1/pk and Solvent system were 0.1% Trifluoroacetic acid: Methanol (60:40). The effluents were observed at 272nm in UV detector. The Rf value of Berberine, Chloroform fraction of Cissampelos pareira roots was 0.23, and the Tinospora cordifolia stems was 0.63. The valid UPLC method parameter for BBE is system precision, SD (5433.07), the Regression equation was found y = 20570 x−182430, Correlation coefficient (R2) 0.9993. The adequate Linearity concentration was found to be 50 to 150µg/ml, LODs (4.02µg/ml), LOQs (12.17µg/ml), Method precision for intraday, Interday %RSD (0.7), (1.4), SD (16064.5), (32811.5), Recovery 98%, and 102%. BBE content was found in a formulation (“KabusuraKudineer” churna- 20.9360µg/ml, Developed Hydroalcoholic Polyherbal formulation - 21.4370µg/ml), and the Chloroform fraction of Cissampelos Pareira roots (CHFCP), Chloroform fraction of Tinospora cordifolia stems (CHFTC) was 28.9090µg/ml and 73.2050µg/ml. The developed liquid chromatography (UPLC) methods have enabled simple, rapid, easy, accurate, reproducible, and linear analysis of BBE in Chloroform fraction of velvet roots, Tinospora cordifolia stems, and Ayurvedic, Siddha -based Polyherbal formulations.