Abstract In this research work, human fingernails were utilized to estimate the radiation dose using EPR measurements of radiation-induced radicals. The limiting factors in this research were the presence of mechanical induced EPR signals due to the mechanical stress during the preparation of the samples. The mechanically induced signals overlap with the radiation-induced signal, which considerably can overestimate the dose. Thus, different treatment methods of fingernails were used to reduce the mechanical induced signals. The results demonstrate that the mechanical and radiation induced signals have apparently different microwave power saturation behavior. Also, mechanical induced signal shows a fading evolution vs. time and reaches to a constant value. Chemical treatment using the different reagents showed that the minimum mechanical induced signal was obtained using the dithiothreitol reagent. The dose response curves of the treated samples with dithiothreitol for 30 min demonstrated a more linearity, and closer curves in comparison to those treated in 5 min. Therefore, to find out an unknown absorbed dose in a fingernail sample using a calibration curve, it could be recommended to use the mentioned chemical treatment procedure to reduce the uncertainty.
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