Dairy Goat Milk Research Articles

Current goat milk production, characteristics, and utilization in Indonesia

In Indonesia and some southeast Asian countries, the number of goats raised for milk production is growing. In these countries, goat milk is the second most produced and consumed milk after cow milk. In Indonesia, dairy goats’ development is promising, but the farming of dairy goats and marketing milk products is still scattered and unstructured. The milk is produced from varieties of goat breeds, including Peranakan Etawa, Saanen, Nubian, and their crosses. Goat milk has unique and distinct characteristics and is an excellent source of nutrients for humans. Compared to cow milk, it has smaller fat globules, more homogenous, shorter rennet coagulation time, and softer curd. Goat milk utilization includes fluid milk for direct consumption; frozen fresh milk; dried or powdered milk; fermented milk products such as yogurt, kefir, including its derivatives such as cosmetics, concentrated yogurt, yogurt cheese, ice cream, shakes; cheeses such as fresh cottage-type cheese, acid-coagulated cheese and mozzarella; and also, other traditional products such as sweets & candies and caramels. The development of dairy goats and goat milk consumption is driven by several factors, including the low cost for start-up and production, consumers’ demand, alleged health properties of goat milk, and varieties of products.

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Dairy goat farming is a significant source of income, particularly for low-income families. However, 75% of the dairy goat products business is still unorganized in Kenya, making its marketing difficult. This study surveyed dairy products in selected supermarkets in Nyeri, Meru and Kiambu counties. The purpose was to identify the types of dairy products sold in supermarkets, the sales preferences, reasons for stocking and not stocking the dairy goat products and perceptions about the dairy goat products. The study targeted all the supermarkets that sold dairy products, excluding those that did not sell dairy products or were not willing to be surveyed. A sample of 40 supermarkets and 5 milk dealers in the three counties was obtained through purposeful sampling. Close-ended questionnaires were administered to managers or appointees of the outlets. According to the findings, 10 types of dairy products were stocked by the outlets as follows: Raw cow milk (11%), pasteurized cow milk (87%), long life cow milk (82%), cow milk yoghurt (93%), ghee (24%), cow mil ice cream (67%), cow milk butter (31%), cow milk cheese (36%), cow sour milk (76%) and pasteurized goat milk. Out of the 45 supermarkets/milk vendors surveyed only 5 outlets sold the dairy goat products (pasteurized milk) which thus indicate the gap in the marketing of dairy goat milk and its products in the study counties. The dairy goat products were not stocked because the products were not available (63%), lack of customers’ awareness (40%), they were not preferred by customers (18%) and high price (10%). The dairy goat milk was stocked due to customers’ preferences (100%). The general perception of the supermarkets was that dairy goat milk was moderately preferred (36%), highly (4%) and not preferred (60%) mainly due to lack of customers’ awareness. Thus, measures should be put in place to promote dairy goat milk as an alternative to dairy cow milk due to its high nutritive value and potential. Key words: Dairy goat products, marketing, supermarkets, sales, preferences, stocking.

KUALITAS FISIK SUSU KAMBING PERAH YANG DISUPLEMENTASI DENGAN SOYBEAN MEAL (SBM)

The study aimed to determine the effect of soybean meal supplementation in the ration on physical quality, namely specific gravity, pH, reductase, and dairy goat milk alcohol tests. The research was conducted from July to Agust 2021 at the Telaga Rizky Ranch owned by Mr. Setiono Heri Winarko, Yosodadi Village, East Metro District, Metro city. The samples used in this study were 12 Sapera goats that were lactating and grouped based on body weight. This study used a randomized block design (RBD) method with 4 treatments and 3 replication, namely P0 (control), P1 (soybean meal 5%), P2 (soybean meal 10%), P3 (soybean meal 15%). The data obtained were than analyzed by variance (ANOVA). The results showed that there was no significant effect (P>0.05) of the treatment on the physical test of diary goats’s milk supplemented with soybean meal. The result showed that the physical test of milk, namely for the specific gravity test was 1.0297 g/cm3, pH was 6,8, reductase was 8,0 hours, and alcohol test was negative. The physical quality of Sapera goat’s milk has good quality and is in accordance with the Indonesian Nasional Standard (2011).
 
 Keywords: alcohol, specific gravity, pH, reductase, soybean meal

Effects of spent coffee grounds on production traits, haematological parameters, and antioxidant activity of blood and milk in dairy goats

Spent coffee ground (SCG) is a byproduct of coffee beverage preparation and a potential source of carbohydrate, protein, and phenolic compounds for livestock feeding. In this study, the effects of SCG supplementation in the diet of lactating goats on milk production traits and health status were studied. The antioxidant status of blood and milk was also evaluated. Twenty-four Saanen goats were fed a total mixed ration containing commercial concentrate, soybean, and haylage; they were divided into three groups: control diet (CON), SCG50 (50 g/d SCG), and SCG100 (100 g/d SCG). The experiment lasted 6 weeks. Linear and quadratic contrasts were used to evaluate the effects of the byproduct doses. SCG supplementation did not affect milk production, but influenced some milk fatty acids. SCG supplementation increased the contents of C18:1, cis-9, trans-11 C18:2, odd and branched-chain fatty acids, and total conjugated linoleic acid. Most of the haematological and biochemical parameters were within the physiological range for goats. The basophil, eosinophil, and glucose contents were quadratically affected by SCG, whereas platelet count increased linearly with the SCG dose. The SCG supplementation had a positive effect on the blood antioxidant status, as evidenced by an increase in ferric reducing antioxidant power and a decrease in malondialdehyde. The SCG supplementation had no effect on the milk antioxidant status. The results show that SCG (up to 100 g/d) did not negatively affect milk production and health status in goats. However, quadratic effects on some antioxidant and biochemical parameters suggest that further investigations are necessary, especially with regard to the optimisation of the supplement dose.

Orf Virus Detection in the Saliva and Milk of Dairy Goats.

Orf is a zoonotic and highly contagious disease caused by Orf virus (ORFV) infection. Orf outbreaks in sheep and goats usually lead to high culling rate and mortality in newborn kids and lambs, posing a great threat to the development of goat and sheep industry. Human Orf occurs via direct contact with infected animals or fomites. While this disease is traditionally thought to spread through direct contact, whether other transmission routes exist remains unclear. Herein, we report the detection of ORFV in the saliva and milk of dairy goats without clinical Orf symptoms. Further analyses showed that these ORFV are infectious, as they can induce characteristic cytopathic changes in primary mammary and lip cells. Importantly, these ORFV can induce typical Orf lesions after inoculation in ORFV-free dairy goats. This is the first study showing that live, infectious ORFV can be isolated from the saliva and milk of asymptomatic goats, highlighting novel potential transmission routes of ORFV. These findings provide a novel idea for the prevention and control of Orf spread.

OPTIMIZATION OF QUALITY AND PRODUCTIVITY OF DAILY GOAT BUSINESS GROUP IN BLIMBING VILLAGE, REJOTANGAN DISTRICT, TULUNGAGUNG REGENCY THROUGH TECHNOLOGICAL INNOVATION

"Karya Putra Mandiri" Livestock Farmer Group and "Makmur Sentosa" Livestock Group, Kec. Rejotangan Kab. Tulungagung is a foster partner of the Unair FKH Community Service Team. A common problem for breeders and administrators of the "Karya Putra Mandiri" Livestock Farmer Group and "Makmur Sentosa" Livestock Group, Kec. Rejotangan Kab. Tulungagung is a problem in production, among others are reproductive disorders, low pregnancy rates, low productivity in their dairy goats. The solution offered is to increase the skills and knowledge of dairy goat farmers in the "Karya Putra Mandiri" and "Makmur Sentosa" livestock groups on how to handle reproductive cases through synchronization of lust. Another problem is the "Karya Putra Mandiri" Livestock Farmer Group and the "Makmur Sentosa" Livestock Group, Kec. Rejotangan Kab. Tulungagung is a management problem. The solution offered is to increase the knowledge and assistance of dairy goat farmers in the “Karya Putra Mandiri” and “Makmur Sentosa” livestock groups in dairy goat milk processing, packaging and product licensing. The method used in the implementation of this community service activity is by; (1) Development and training stage; (2) Monitoring and evaluation stage. To evaluate the level of knowledge and understanding of the material given, pretest and posttest are given. This Community Service program activity was carried out from March to October 2018. Extension activities and training on Laserpuncture and Sexual Synchronization Technology were carried out using the tutorial method, then continued with discussions. The implementation of this Community Service Activity requires a budget of 30 million rupiah which is planned to be used in several posts including for equipment, consumables, travel, accommodation, and others.

The Impact of Dietary Berberine Supplementation during the Transition Period on Blood Parameters, Antioxidant Indicators and Fatty Acids Profile in Colostrum and Milk of Dairy Goats.

The objective of this study was to investigate the effect of berberine (BBR) supplementation on productivity, antioxidant markers, and the fatty acid (FA) profile in the colostrum and milk of goats. Twenty-four primiparous Saanen goats were supplemented with 0, 1, 2, and 4 g/d (per goat) of BBR in control (CON), BBR1, BBR2, and BBR4 groups (n = 6 per group), respectively, from 21 days before expected kidding to 21 days after parturition. Blood sampling was carried out at −21, −14, −7, 0, 7, 14, and 21 d relative to delivery. Colostrum was collected within the first and second milking (d 1 of lactation), and milk was harvested weekly after kidding. Both BBR2 and BBR4 increased dry matter intake (DMI) (p ≤ 0.05) and energy balance (EB) as well as colostrum and milk production. Both BBR2 and BBR4 decreased (p ≤ 0.05) plasma levels of cholesterol, haptoglobin, and ceruloplasmin, while elevating the plasma albumin and paraoxonase (p ≤ 0.05), which may indicate that BBR mitigates inflammation during the transition period. BBR reduced (p ≤ 0.05) malondialdehyde (MDA) and increased (p ≤ 0.05) total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in blood, colostrum, and milk. Concentrations of de novo fatty acid in colostrum and milk were increased (p ≤ 0.05) with both BBR2 and BBR4. Free fatty acid (FFA) concentration in colostrum and milk fat were lower (p ≤ 0.05) in BBR2 and BBR4 compared to CON. The concentration of saturated fatty acids (SFAs) in colostrum and milk fat increased (p ≤ 0.05) with BBR2 and BBR4, while unsaturated fatty acids (USFAs) decreased (p ≤ 0.05) in milk. In summary, supplementation with at least 2 g/d BBR may enhance the EB and antioxidant status of dairy goats.

Mammary gene expressions and oxidative indicators in ruminal fluid, blood, milk, and mammary tissue of dairy goats fed a total mixed ration containing piper meal (Piper betle L.)

The study evaluated the effect of piper meal which contains flavonoids, essential oils, and phenolic acids on the activity of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and as well as oxidative stress indicators such as 1,1-diphenyl-2-picrylhydrazyl-scavenging activity and thiobarbituric acid-reactive substances (TBARS) in the ruminal fluid, mammary tissue, milk, and blood of lactating goats. Fourteen early lactating Saanen goats (body weight, 44 ± 2.51 kg; 14 ± 3 days in milk) were allotted in equal number to two experimental groups, each received one of the two total mixed rations: control (CON) diet (containing 0% piper meal) and CPM diet (CON diet containing 1.3% piper meal per kg dry matter). Compared with goats fed the CON diet, those fed CPM diet had similar 1,1-diphenyl-2-picrylhydrazyl-scavenging, GPx and CAT activity in ruminal fluid, mammary tissue, milk, and blood; however, those samples had increased SOD activity. Dietary CPM diet had a tendency to decrease TBARS production in ruminal fluid, mammary tissue, milk, and plasma by about 2.4, 1.2, 1.1, and 1.4 folds, respectively. Furthermore, alleviated values of TBARS were associated with dwindling mRNA expression of κ light polypeptide gene enhancer in nuclear factor kappa B subunit 1 (NFKB1) but greater expression of SOD1, SOD2, SOD3, and nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 ) in mammary tissue. Our findings showed that the inclusion of 13.0 g piper meal in per kg diet (CPM) can improve the oxidative status of Saanen goats in early lactation.

ХАРАКТЕРИСТИКА КОРРЕЛЯЦИОННЫХ СВЯЗЕЙ МЕЖДУ КОМПОНЕНТАМИ МОЛОКА КОЗ МОЛОЧНОГО И КОМБИНИРОВАННОГО НАПРАВЛЕНИЙ ПРОДУКТИВНОСТИ

Dairy goat breeding is developing dynamically in the world and in Russia. To scientifically substantiate the breeding strategy of dairy goats, meat-dairy goats and dairy-meat ones, it is necessary to expand information about the composition of goat milk and the nature of correlations between its components. In the article, the composition of goat milk of dairy goats, meat-dairy goats and dairy-meat ones is considered in the comparative aspect. The correlation coefficients between the indicators studied by infrared spectroscopy and flow cytometry using automatic CombiFoss 7 DC analyzer are presented. It was found that milk of dairy-meat and meat-dairy goats significantly exceeded milk of dairy goats in terms of the mass fraction of fat, protein, levels of saturated and unsaturated fatty acids, lactose and urea. At the same time, the level of differential cells was significantly lower. The nature of the relationship between mass fraction of fat and other components of milk in goats with different severity of milk productivity was the same both in strength and in orientation. A high positive relationship (r = 0.65…0.99) was revealed between the mass fraction of fat and the content of fatty acids, both saturated (SFA, LCFA, MCFA) and unsaturated (MUFA, PUFA, SCFA). Differences in the nature of the relationship between the content of casein and lactose, casein and DSCC were established. For milk of dairy-meat and meat-dairy goats, it was low negative (r = –0.27 and –0.31), for milk of dairy goats – low positive (r = 0.29 and 0.28). Differences were also found between mass fraction of true protein and fatty acids, MDB and the level of somatic cells. Mass fraction of true protein in milk of dairy goats was weakly negatively correlated with LCFA, SCFA, KSK and DSCC (r = –0.16…–0.32), in milk of dairy-meat and meat-dairy goats this relationship was weakly positive (r = 0.14…0.26).

Effect of Increased Salt Water Intake on the Production and Composition of Dairy Goat Milk.

Simple SummaryThe high consumption of water by livestock is a major drawback from an environmental point of view. In addition, the fact that arid or semi-arid areas have water with high salinity can decrease the quality of the products derived from these animals. Therefore, the present work is proposed with the objective of understanding how the supply of water with different salinities can affect the quality of milk. Therefore, the results of this work will derive important conclusions of vital importance for farmers in these areas.Due to its necessity and magnitude, water is essential for animal nutrition. This study aimed to evaluate the effects of increasing levels of water salinity on the quality of goat milk in the Brazilian semiarid region. Twenty-four multiparous Alpine goats, with an average live weight of 38.0 ± 4.0 kg and an average lactation period of 30 days, distributed entirely at random, were used. The experiment lasted 64 days including an initial period of 14 days of adaption to the diet. The experimental treatments consisted of water with different levels of total dissolved solids (TDS): 640, 3188, 5740, and 8326 mg L−1, obtained using sodium chloride (NaCl). Increasing the levels of TDS in drinking water from 640 to 8326 mg L−1 did not significantly (p > 0.05) affect the production and the physicochemical composition of the milk. There was a linear increase (p < 0.05) in the water consumption and acidity variables as a function of the total dissolved solid levels. The mineral composition of the milk was not altered with increasing levels of TDS in water from 640 to 8326 mg L−1. There was no negative effect (p > 0.05) for any of the sensorial attributes analyzed in relation to the treatments. Therefore, as a general conclusion, based on the analyses carried out in this experiment, it was found that water with total dissolved solids, when supplied for short periods of up to 48 days, does not alter the production, physicochemical characteristics, or the organoleptic properties of goat’s milk.

Fat- and protein-corrected milk formulation to be used in the life-cycle assessment of Mediterranean dairy goat systems

The aim of this paper is to develop a new specific algorithm (fat- and protein-corrected milk calculation) for dairy goat to be used in life-cycle assessment (LCA) studies. Though the contribution of goat milk to world milk production ranks third, the literature does not report any specific correction for goat's milk. Using the available bibliographic data, a multiple regression was performed that allowed obtaining the relationship between the energy content (EC) and the fat (FC) and protein content (PC) of dairy goat milk. The multiple regression resulting from the 3 variables analyzed through the data drawn from the literature was significant (R2= 0.99; p ≤ 0.001). The equation resulting from the correlation was used to develop algorithms for the calculation of fat- and protein-corrected milk (FPCM) at specific FC and PC. Since FC and PC are very different depending on the goat breed two different groups have been defined: i) goat breeds with FC in milk below 4% (FCB4), and ii) goat breeds with FC in milk above 4% (FCA4). The EC found for the FCB4 group (FC = 3.70 and PC = 3.27) was 728.11 kcal kg−1, while that of the FCA4 group (FC = 4.92 and PC = 3.61) was 860.69 kcal kg−1. After substituting the EC values obtained before, the resulting FPCM equations to calculate specific FC and PC by unit of mass (kg of milk = M), according to each group, are shown below: FCB4 group = FPCM (FC = 3.70, PC = 3.27) = M * [(0.12 * FC + 0.10 * PC + 0.23)]; FCA4 group = FPCM (FC = 4.92, PC = 3.61) = M * [(0.10 * FC + 0.08 * PC + 0.20)]. Finally, the variation between the FPCM values calculated using the specific equation obtained in this study for goats and those previously published (for sheep or modifications made from dairy cattle) was evaluated; in FCB4 group the differences varied between (-) 32% and (+) 14% and in FCA4 group between (-) 21% and (+) 35%. Values are overestimated if sheep's FPCM calculations are used (because sheep milk has higher fat content than goat milk) and underestimated if dairy cattle's FPCM calculations are used (because cow milk has lower fat content than goat milk). In conclusion, the results found in the present study show the need to use a specific FPCM formulation in the LCA equation for goat's milk.

LC-Q-Orbitrap HRMS-based proteomics reveals potential nutritional function of goat whey fraction

• Large variations in protein composition were observed between goat and bovine milk. • Abundance of GSN, SERPINA1, C3, and APOA1 were high in goat mature milk. • Functional analysis of the identified protein revealed potential biological function. • These results expand the direction for dairy-based functional food exploitation. Guanzhong dairy goat milk is the product of national geographical indication exclusively in Shaanxi province of China. A total of 433 whey proteins were identified and quantified in colostrum and mature milk, which were obtained from Guanzhong dairy goat and Chinese Holstein. Twenty-seven highly expressed proteins in GM were related to immune response, and 23 were metabolic processes. Thirty-four highly expressed proteins in GC milk were mainly related to the immune response, negative regulation of endopeptidase activity and ossification, indicating that goat milk was very important for the development of immune system and bone growth and ossification in newborns. Bioinformatics analysis showed that the differentially expressed proteins in the four whey groups were mapped in 15 pathways, in which the complement and coagulation cascade and carbon metabolism were the most important. Cross-species comparison of whey protein helps to extend understanding of variation in the nutritional value of different milk sources.

Evaluation of Different Test-Day Milk Recording Protocols by Wood's Model Application for the Estimation of Dairy Goat Milk and Milk Constituent Yield.

Simple SummaryConsidering the seasonal trend of milk production due to application of estrus synchronization treatments in autumn or in spring season, as well as to meet meat production and market requirements for religious holidays, one may find it useful to simplify the milk and milk constituent yield quantification during the entire period of lactation. The present work aimed to verify the application of Wood’s model to a different number of test-day milk recording protocols in order to reduce the frequency of National Breeding Association test-day milking record protocol. Wood’s model fit well on goat milk production, showing high R2 values. Moreover, the milk yield, fat-corrected milk yield, fat yield, and protein yield estimation applying Wood’s model to four recordings (first, second, fourth, and sixth month records) showed results superimposable to International Committee of Animal Recording (ICAR) application and Wood’s model application on test-day records every 15 days.Goats have important social and economic roles in many countries because of their ability to survive and be productive in marginal areas. The overarching aim of this study was to compare the application of Wood’s model to different test-day milk recording protocols for estimation of total milk, fat, and protein yield in dairy goats. A total of 465 goats were used (Garganica, 78; Girgentana, 81; Jonica, 76; Maltese, 77; Red Mediterranean, 76; Saanen, 77). Milk yield was recorded every 15 days throughout lactation of 210 days, for a total of 14 collection days, during both morning and afternoon milking sessions. Milk samples were collected and analyzed for protein and fat. The fat-corrected milk was standardized at 35g fat/kg of milk. Wood models showed high R2 values, and thus good fitting, in all the considered breeds. Wood model applied to first, second, fourth, and sixth month recordings (C) and ICAR estimation showed total milk yield very close to Wood’s model applied to all 14 recordings (A) (p > 0.38). Differently, Wood’s model applied to the first, second, third, and fourth month recording (B) estimation showed great differences (p < 0.01). This could be applied for farms that had the necessity to synchronize flock groups for kidding in order to produce kid meat. In farms that apply the estrus induction and/or synchronization for kidding, it would be possible to perform only four test-day milk recordings and to apply the Wood’s model on them in order to obtain the estimation of total milk, fat, and protein yield during lactation for animals inscribed, or to be inscribed, to the genealogical book.

Investigations on the excretion of antibiotics in goats' milk following therapeutic application

Data on the excretion of antibiotic residues following therapeutic drug dosages in lactating goats with clinical signs of bacterial infections are currently lacking. Therefore, this study aimed at monitoring the drug residue excretion of a subset of frequently used antibiotics in the milk of dairy goats following their therapeutic administration. Over a period of 4 months, milk samples (udder halves) were collected in 2 goat milk farms from animals treated with antibiotics in routine practice based on the diagnosis of a bacterial infection. The samples were examined up to 3 days following the withdrawal time point. The animals were classified in 3 groups depending on their clinical symptoms and treatment. Goats in group 1 (afebrile goats with various bacterial infections excluding the udder) were treated with intramuscular amoxicillin injection (n = 5). Animals in group 2 (mastitis catarrhalis) were treated with intramammary injection of oxacillin and ampicillin (n = 6). Group 3 consisted of a single goat diagnosed with mastitis. This individual was treated with cefquinome in accordance with the results of the antibiogram. Milk samples were examined qualitatively by using a receptor assay (Betastar®) as well as a microbiological inhibitor assay (Brilliant black reduction test, BRT). The latter assay was also used to semiquanti-tatively analyse drug residue levels in samples from group 2. Following intramuscular treatment with amoxicillin, drug residue levels were estimated to be very similar in both udder halfs. Elimination was complete 3 days after the end of the treatment period. Animals in group 2 showed significant differences between treated and untreated udder halves. However, the untreated halves still exhibited residue levels exceeding the maximum residue limits during the treatment period. In both group 2 and 3, all milk samples were tested negative for drug residues before the end of the withdrawal period. In the present study, no evidence of prolonged residue excretion into milk of goats following therapeutic administration of antibiotics was detected. Both the receptor test and the BRT represent suitable methods for an efficient antibiotic drug residue testing in goat milk. Reliable testing was merely not attainable in cases of milk samples possessing highly altered organoleptic characteristics.

Assessment of the Sustainability of Extensive Livestock Farms on the Common Grasslands of the Natural Park Sierra de Grazalema

The communal pastures of the Natural Park Sierra de Grazalema are grazed by a total of 23 extensive herds, of which 75% are certified as organic, although only 39% are subsidized for being organic. In a previous research work, these farms were characterized and classified into four typologies: group 1 (farms of intermediate size and without sheep), group 2 (large and very extensive farms), group 3 (farms with sheep suitable for both meat and milk) and group 4 (farms with dairy goat milk and without cattle). In this article, the sustainability of these farms is evaluated and compared based on their organic orientation (whether they are organic or conventional) and their typology (the four typologies indicated), as a tool for decision-making in the management of this natural protected area. To do so, 49 sustainability indexes have been generated, grouped into five attributes: adaptability, self-management, equity, stability, and productivity. The results indicate that, at the global level, there are no significant differences in sustainability between the organic and conventional farms studied. In contrast, depending on the typologies, the results indicate that group 3 is the most sustainable, followed by groups 1 and 4, with group 2 being the one with the lowest level of sustainability. Taking into account that there are a reduced number of herds grazing in this natural park, it is essential to solve the weaknesses of these farms in order to guarantee that they continue to maintain environmental equilibrium in the grasslands.

Compositional and Functional Comparisons of the Microbiota in the Colostrum and Mature Milk of Dairy Goats.

Simple SummaryOur findings revealed significant influences of lactation stage on goat milk microbial and functional composition both in colostrum and mature milk. Microbial communities which were found in colostrum and mature milk could potentially establish goat kids’ gut microbiota and have an influence on the development of microbial components that provide potential health promotion effects in goats.Goat milk is essential for the initial development of kids by providing a great source of commensal bacteria. In this study, we analyzed the microbiota of the milk of 30 healthy Saanen dairy goats. The 30 samples comprised 15 colostrum and 15 mature milk samples, collected from three different farms of Shaanxi Province. Colostrum samples were collected daily for five days post-delivery and mature milk was collected on the 7th, 10th, 20th, 30th, and 40th days. The result showed that microbial alpha diversity was higher in the mature milk compared with that in the colostrum. Linear discriminant analysis effect size (LEfSe) was performed to detect differentially abundant taxa in colostrum and goat milk. According to taxonomy results, Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the predominant bacteria phyla in both colostrum and mature milk. In addition, lactation stage noticeably influenced the composition of milk microbiota. Specifically, Novosphingobium, Brachybacterium, Psychrobacter, Lactobacillus, Yersinia, Roseateles, Rothia, Sanguibacter, Cloacibacterium, Variovorax, Sphingobacterium, and Coxiella were enriched in the colostrum, while Georgenia, Peptostreptococcus, Bacteroidales, Yaniella, Planomicrobium, Cloacibacterium, Azospirillum, Turicibacter, Cupriavidus, Herbaspirillum, Rhodobacteraceae, and Aeromonadales were the dominant genera in the mature milk. The enriched metabolic functions of the goat milk microbiota were predicted by PICRUSt and classified by KEGG pathway. Moreover, the abundances of environmental information processing, cellular processes pathway, genetic information processing pathway, organismal systems pathway, and metabolism pathway were significantly different between microbiota of colostrum and mature milk. Altogether, our study disclosed the significant difference between the microbial communities of colostrum and mature milk and provided grounds for further research in dairy microbiology.

Milk As a New Diagnostic Tool for Rapid Detection of Fascioliasis in Dairy Goats Using Excretory/Secretory Antigen

Fascioliasis is a serious livestock illness of particular importance for dairy goats; the objectives of this study were to describe effects of F. gigantica on milk composition and to use this information to estimate economic damages linked with Fasciola spp. infections. Furthermore, the study sought to standardize the use of milk instead of serum for early diagnosis of fascioliasis in dairy goats. One-hundred samples of goat milk along with corresponding blood samples were obtained at random from flocks in Cairo and Giza Governorates. The ELISA and DOT-ELISA were performed in both serum and milk of dairy goats. Total mesophilic count (mean value) was 2.12 × 106 ± 1.63 × 105CFU/ml in enzyme-linked immunosorbent assay (ELISA) positive samples and 1.46 × 104 ± 8.58 × 102CFU/ml in ELISA-negative samples. The mean values were significantly different (P < 0.05). The mean values of percentages of fat, SNF, protein, salts, lactose, pH, and MSCC/ml in ELISA-positive samples were 2.3 ± 0.17, 8.21 ± 0.63, 3.08 ± 0.18, 0.90 ± 0.06, 3.64 ± 0.28, 6.93 + 0.53, and 1.18 × 106 ± 9.07 × 104cells/ml, respectively. A significant difference (P < 0.05) between the mean values of two composition parameters, i.e., percent of fat and MSCC/ml in ELISA-positive and -negative samples, for Fasciola gigantica was observed. The antigen used for the diagnosis of F. gigantica was excretory/secretory (E/S) antigen. The dilutions of (E/S) concentrations after checkerboard titration for indirect ELISA were 20μg/ml protein and for dot-ELISA, 300ng/μl. Sera dilution was 1:100 in the two tests, and milk dilution was 1:50 for indirect ELISA, and 1:25 for dot-ELISA. The two tests were performed using known F. gigantica positive and negative goat sera and known rat hyper immunized negative and positive sera against E/S antigen of F. gigantica as well as known sera for paramphistomes without F. gigantica infection. The cutoff values in indirect ELISA were 0.45 for sera and 0.35 for milk. The application of different serological technique in goat farms reveals a good test in rapid diagnosis of fascioliasis especially the uses of dot ELISA when using the milk instead of the serum.

DETERMINATION OF AFLATOXIN M1 IN RAW MILK PRODUCE AT YOBE STATE UNIVERSITY FARM DAMATURU, NIGERIA

The study was carried out to determine the contamination of aflatoxin M1 (AFM1) in samples (n=66) of raw milk, from three distinctive animal species (cow, n = 30; goat, n = 20; sheep, n = 16) at Yobe State University farm Damaturu in 2018. The analytical strategy utilized was high-performance liquid chromatography (HPLC). Immunoaffinity columns were used to achieve clean–up step during HPLC and fluorometric determination. The outcomes demonstrated that 36 (54.54%) samples out of the 66 samples are debased with AFM1. The sullying rates of AFM1 in dairy animals, goat milk and sheep milk were 80.0%, 25.0% and 46.75% respectively. The mean concentration for the cow, goat and sheep milk was 0.1333µg/l, 0.0462µg/l and 0.0519µg/l respectively. The general mean convergence of AFM1 levels for positive samples from the three distinctive species was 0.0727 µg/l and there was no huge contrast (p = 0.3624) in fixation levels between the three species. The estimated intake (EDI) of AFM1 from consumption of cow milk products by teachers and the students was 0.00158g/kg b.w/day based on one-day recall methods, while hazard index was recorded to be 1.58 x10-4. The high levels of AFM1 concentration recorded in this study is an indication of contamination by the fungus during storage of feeds, this may have negative effects on the human and animal’s health since it’s proven to be carcinogenic, causes growth impairment and immune suppression. Measures should be enforced on the storage of feeds which will consequently decrease the odds of aflatoxin in milk of

Determination of the milk pharmacokinetics and depletion of milk residues following intramammary administration of cephapirin sodium in lactating dairy goats

In the United States, between 2007 and 2017, the US Department of Agriculture’s National Agricultural Statistics Service reported dairy operations that had milk goats to be the fastest growing operations in the nation. The most impactful and economically important health challenge to dairy goat herds is mastitis. Currently, there are no labelled products for the treatment of mastitis in dairy goats and minimal research has been conducted to evaluate the pharmacokinetics of intramammary-administered antimicrobials. The objectives of this study were to determine the pharmacokinetics in milk of dairy goats treated with intramammary (IMM) cephapirin sodium and determine an appropriate withdrawal time for milk.

Isolation and characterization of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) from milk of dairy goats under low-input farm management in Greece

The presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk is a challenge for veterinarians and public health professionals. In this study, we investigated the presence and clonality of S. aureus and MRSA in milk of individual dairy goats with subclinical mastitis reared under the low-input farming system in Greece and determined the isolates’ enterotoxin gene carriage and their ability to form biofilms. S. aureus was isolated from 162 out of the 559 milk samples examined (29 %) and one isolate per S. aureus-positive sample was further characterized. S. aureus isolates were very closely related even among farms of distant geographical regions. Nine S. aureus isolates carried a functional mecA gene and were classified as MRSA. The S. aureus protein A (spa) typing in the MRSA isolates showed that four belonged to spa type t127 (44.4 %), three to t2049 (33.3 %) and two to t7947 (22.2 %). The spa type t7947 is reported for the first time in Greece. The MRSA isolates originated from two very distantly located farms, one located in the island of Skopelos and the other in Central Macedonia. Four of the MRSA isolates carried the staphylococcal enterotoxin genes sea or sec. Most of the isolates (92 % of S. aureus and 77.8 % of the MRSA) possessed moderate or weak biofilm-formation ability. Raw milk from low-input goat herds may serve as a potential vector of antimicrobial-resistant S. aureus to raw-milk consumers.