Metal Organic Framework MIL-101 Research Articles

Efficient purification of metal-organic framework and its trigger strategy in battery thermal management system

Metal-organic framework (MOF) MIL-101(Cr) is a sorbent that can adsorb large amounts of water, but its performance is compromised by low thermal conductivity and unstable desorption temperature. In addition, the high cost restricts its application in thermal management. We propose a novel purification method, which reduces the cost of MIL-101(Cr) by 91%. Then, based on MIL-101(Cr) and micro heat pipe array (MHPA), we design an innovative thermal management structure with great performance, MHPA@MIL-101(Cr). To cope with extreme conditions, a trigger strategy based on active dehumidification and air-cooling technologies is used to improve the temperature and temperature uniformity of cells, and the effect of their trigger time on the desorption temperature of MIL-101(Cr) is studied. In a normal condition (25 °C), the zero-energy-consumption structure controls the maximum temperature (Tmax) of the battery module below 35.99 °C at 2C rate (112A). What is more, the trigger strategy improves the temperature characteristics in an extreme condition (35 °C). As a result, the Tmax is controlled at 38.8 °C when the battery module is 2C (112A) discharged, a temperature rise of only 3.8 °C.

Determination of Aflatoxins in Peanut Oil by One-Step Solid-Phase Extraction with Metal-Organic Framework MIL-101 (Cr) Filled Syringe Filter and HPLC-MS/MS

A simple, rapid and sensitive method for the determination of aflatoxins in peanut oil was developed using MIL-101(Cr) material filled syringe filter for one-step solid-phase extraction (SPE) with high-performance liquid chromatography—tandem mass spectrometry (HPLC-MS/MS) detection. The MIL-101(Cr) sorbent was characterized and its mass, the loading solution, and the eluent were optimized; under these conditions, a matrix matching internal standard method was developed. The linear range was from 0.1 to 20 ng/mL with correlation coefficient (r) exceeding 0.9998. The recoveries were between 94.7% and 105.9% with relative standard deviations from 2.02% 6.88%. The recoveries of inter-day and intra-day measurements were from 93.0% to 98.5% and 97.6% to 100.7%. The precision values for inter-day and intra-day analyses were from 6.09% to 7.68% and 2.56% to 4.30%, respectively. The pretreatment efficiency of the developed method was superior to literature procedures and used to determine aflatoxins in 126 peanut oil samples from local markets. 38 samples had positive results and 1 sample exceeded the allowable limit. Hence, the developed protocol was suitable for the determination of aflatoxins in peanut oil.

A sampling platform incorporating 3D-printed paddles-stirrers coated with metal-organic framework MIL-100(Fe) for in-situ extraction of phenolic pollutants in biodigester supernatant and wastewater effluent samples

In this work, a portable paddle stirrer platform for solid-phase extraction of chlorophenols, p-nitrophenol, and bisphenol A is presented. These compounds are important environmental pollutants and toxic inhibitors of the anaerobic treatment of wastewater. The platform integrates 3D-printed paddle stirrers coated with the metal-organic framework MIL-100(Fe), which is prepared in 10 min using a green microwave-assisted synthesis. This coated stirrer serves as an adsorbent for the preconcentration of phenols. The device is assembled with an electric motor that agitates the sample in a sample container. The sampler can be used for in-situ extraction of the analytes, eliminating the need for transporting samples to the laboratory. The analytes are eluted through ultrasonic desorption before analysis by HPLC-DAD. An exhaustive study of the stirring and extraction parameters was conducted. Under optimum conditions, the detection limits ranged from 0.3 to 1.7 μg L−1, and the precision, expressed as the relative standard deviation, showed intraday and interday ranges of 1.2–5.1% and 4.5–6.8%, respectively. The accuracy was evaluated using spiked samples of wastewater effluent and biodigester supernatant, and it provided relative recoveries in the range of 91.5–108.5%.

MIL-100(Fe)/Diatomite Composites for Photo-Fenton Degradation of Phenol

Porous materials based on metal-organic framework MIL-100(Fe) and diatomite were synthesized. Composites possess specific surface area of 322 and 441 m2/g and hierarchical porous structure with broad pores of diatomite and narrow pores of MIL-100(Fe) particles. Influence of synthesis strategy on structure of materials and their catalytic properties in photocatalytic degradation of phenol was investigated. Composite obtained with preliminary wet impregnation of iron nitrate solution show predominant formation of MIL-100(Fe) particles inside the pores of diatomite. Materials demonstrate catalytic activity in phenol degradation by photo-Fenton process. Composite synthesized without preliminary wet impregnation displays highest catalytic activity with predominant formation of MIL-100(Fe) particles on external surface of diatomite.

Cleaner production of metal-organic framework MIL-101(Cr) for toluene adsorption

Metal-Organic Frameworks (MOFs) are a kind of materials with different environmental applications. However, in a sustainable approach, the production must be analyzed from economic, technical, and environmental standpoints. The aim of this study is focused on obtaining a cleaner production of MIL-101(Cr). A multi-criteria decision-making method was applied to consider environmental (Life Cycle Assessment methodology), technical (toluene adsorption experiments), and economic (Monte Carlo simulation) criteria. Optimization of the synthesis process resulted in a MIL-101(Cr) with good textural properties, which behaves as a strong adsorbent suitable for toluene capture (43.44 mmol/g adsorption capacity) at low concentrations (∼2 ppm). The environmental analysis results showed significant impacts in Climate Change > Fossil Depletion > Particulate Matter categories due to toxic solvents and metallic salt. Monte Carlo simulation revealed that toxic solvent also provides an extra cost in MIL-101(Cr) synthesis (30–35 %). Moreover, the results revealed that the cleaner MIL-101(Cr) synthesis presented 64 % minor environmental impact and is 34 % more economical than a conventional MOF synthesis. This research is a step toward a more comprehensive method for synthesizing MOFs for adsorption applications by combining technical, economic, and environmental perspectives.

Conductive metal-organic framework flowers facilitate the anchoring and conversion kinetics of polysulfides for lithium‑sulfur batteries

Enhancing the trapping and catalytic conversion of polysulfides is the main path to restrain their shuttling for durable lithium‑sulfur batteries (LSBs). Herein, we report a flower-like metal-organic framework (MOF) MIL-47 layer as an interlayer material to achieve this purpose. The proposed MOF material possesses a unique flower-like structure that is composed of two-dimensional nanosheets, which is conducive to the mass transfer and the exposure of active sites, enabling an efficient adsorption towards polysulfides. Moreover, the high conductivity combined with sufficient catalytic active centers are instrumental in accelerating the redox processes of polysulfides conversion. Consequently, this flowery MOF-based interlayer endows LSBs an amazing capacity of 1003.6 mAh g−1 and an ultralong cycling stability with a low attenuation rate of 0.029 % over 1000 cycles under 1C, together with an exceptional rate performance (a capacity high as 696.9 mAh g−1 under 5C). In addition to this, an elevated sulfur loading and reduced electrolyte content do not affect its excellent cycling and rate performance. This work provides a new mentality to exploit multi-role nanostructured materials for LSBs.

Enantioselective catalysts based on metal-organic framework-supported nucleotides

Adenosine triphosphate (ATP) and other nucleotides can be irreversibly bound to the metal-organic framework (MOF) MIL-101(Cr). Analysis of X-ray diffraction data suggests that the location of the adsorbed ATP molecule is in proximity of the Cr3 clusters. Solid-state NMR and DFT calculations indicate that ATP is bound to MIL-101(Cr) through linkages of the terminal phosphate group with Cr(III) of the framework. In the presence of Cu(II) ions, the MOF-supported nucleotides can function as stable and reusable enantioselective heterogeneous catalysts for reactions like Diels-Alder and Michael addition. Compared to the corresponding homogeneous nucleotide-based artificial metalloenzymes (ArMs), the MOF-supported nucleotide-based ArMs exhibit significantly enhanced activity and selectivity in certain cases, demonstrating their potential as a new class of enantioselective heterogeneous catalysts.

MIL-100(Fe)-Based Composite Films for Food Packaging.

A biocompatible metal-organic framework MIL-100(Fe) loaded with the active compounds of tea tree essential oil was used to produce composite films based on κ-carrageenan and hydroxypropyl methylcellulose with the uniform distribution of the particles of this filler. The composite films featured great UV-blocking properties, good water vapor permeability, and modest antibacterial activity against both Gram-negative and Gram-positive bacteria. The use of metal-organic frameworks as containers of hydrophobic molecules of natural active compounds makes the composites made from naturally occurring hydrocolloids attractive materials for active packaging of food products.

Amine functionalization of iron-based metal-organic frameworks MIL-101 for removal of arsenic species: Enhanced adsorption and mechanisms

Chronic exposure to inorganic arsenic (As)-contaminated water seriously threatens human health. Although iron-based metal-organic frameworks (MOFs) for arsenic removal have been developed, their practical application has been challenging due to their lack of binding sites and low stability. In this study, we attempted to improve the arsenic adsorption capacity of MIL-101(Fe) in water by amine functionalization. NH2-MIL-101(Fe) was synthesized using a facile solvothermal method. It adsorbed > 70 % arsenate (As (V)) throughout a wide pH range of 4–9, exhibited high anti-interference ability and reusability, and effectively eliminated arsenic from contaminated river samples, demonstrating its great potential in As-polluted water treatment. Best adsorption effectiveness at pH 7 indicated that the electrostatic attraction between -NH2 and As (V) was not its primary adsorption mechanism. Instead, FTIR and XPS analyses revealed that the complexation between Fe and As was the key mechanism. NH2-MIL-101(Fe) had substantially better As (V) and arsenite (As (III)) adsorption capacities (147.7 mg/g and 153.4 mg/g, respectively) than MIL-101(Fe) (82.8 mg/g and 131.2 mg/g, respectively). This is because amine modification increased the Fe content and Fe3+/Fe2+ ratio, as well as the specific surface area and pore volume. This research elucidates how amine modification affects the ability of Fe-MOFs to adsorb arsenic.

Confined construction of CDs@MIL-101 as reusable and turn-on fluorescence sensor for highly sensitive water detection based on adsorption-solvent synergistic mechanism

Fabrication a reusable and highly sensitive fluorescence sensor in a controllable manner for water detection in solvents and humidity remains a top priority target yet a challenging task. In this work, carbon dots (CDs) are successfully implanted into confined metal–organic framework MIL-101(Cr) cages via “ship in the bottle” strategy and the confined CDs are firstly observed in MIL-101(Cr) cage showing high dispersion and high N content by advanced iDPC STEM technique. The highly selective water capture and sensitive changes of the fluorescence property with solution polarity and hydrogen-bonding donor acidity efficiently enhance the detection sensitivity and realize reusable turn-on detection based on the adsorption-solvent synergistic mechanism. Consequently, CDs@MIL-101 exhibits high sensitivity (detection limit: 0.036% and 0.028% v/v), wide response range (0.04–100% and 0.03–45%) and reusable stability for liquid water detection in acetone and tetrahydrofuran, respectively. Meanwhile, it can also detect gaseous water and the detection range is 23% to 85%. The analysis of real samples confirms the proposed method. Such construction strategy and detection mechanism will offer guidance for optimizing and expanding the detection performance of CDs, which is of great significance in the design and construction of novel multiphase sensors.

A multi-enzyme cascade microneedle reaction system for hierarchically MRSA biofilm elimination and diabetic wound healing

The existence of biofilm remains a significant impediment to the healing of chronic wounds, including diabetic wounds. The dense extracellular polymeric substance (EPS) barrier of biofilms leads to poor drug permeability, making biofilms eradication a challenging task. Additionally, high glucose levels hinder the process of wound healing. To address these issues, we developed a multi-enzyme cascade microneedle system (MN) by combining enzymatic biofilm disruption with GOx-based chemodynamic therapy (CDT) for the first time. The microneedle tip dissolves quickly and delivers α-amylase and glucose oxidase-loaded metal–organic framework MIL-101 (M@G) into the biofilm’s interior. The α-amylase disintegrates the EPS structure, making the bacteria vulnerable and producing glucose as a substrate for the next cascade reaction. Next, GOx uses the glucose produced from EPS degradation and present at the wound site to produce significant amounts of H2O2, which is then transformed to reactive oxygen species (ROS) through MIL-101. The system eradicates methicillin-resistant Staphylococcus aureus (MRSA) biofilms by sequentially destroying EPS, consuming glucose at the wound, and self-supplying H2O2 to produce ROS, thereby reducing glucose concentrations and concurrently decreasing bacterial infection and inflammatory responses. Additionally, the pro-angiogenic peptide functionalized hydrogel (Gel-Q-M) serves as a backing to promote collagen deposition and angiogenesis, thereby accelerating the healing of chronic wound. In conclusion, the constructed MN platform showed excellent anti-biofilm and angiogenic properties in vitro, which could effectively eliminate biofilm, reduce inflammation and promote diabetic wound repair.

Magnetic carbonized cellulose-MIL 101(Fe) composite as a sorbent for magnetic solid phase extraction of selected organophosphorus pesticides combined with high performance liquid chromatography-ultraviolet detection

In this research project, an attempt was made to synthesize new composite based on nanomaterials and use these materials to improve the performance of magnetic solid phase extraction for the extraction of some selected organophosphorus pesticides in real samples. Therefore, the metal-organic framework MIL-101 was grown by layer-by-layer growth method on the surface of the magnetic carbonized cellulose nanoparticles. The extracted analytes were measured by HPLC after extraction. The influential variables such as: extraction time, sorbent amount, volume of desorption solvent, desorption time, ionic strength and solution pH were studied and optimized. Under the optimum condition, the linear ranges of the method were obtained in the range of 0.2–200.0μgL−1 (chlorpyrifos: 0.5-200.0μgL−1, profenofos: 0.2-100.0 μgL−1 and phosalone:0.5-200.0μgL−1). The obtained correlation coefficients (r2) were between 0.9966 - 0.9990. The limits of detection were also calculated to be between 0.06 - 0.15μgL−1 (chlorpyrifos: 0.15μgL−1, profenofos: 0.06 μgL−1 and phosalone: 0.13μgL−1) .The relative standard deviations, were obtained in the range of 1.1 to 3.4%. The method was successfully used to extract and measure the selected organophosphorus pesticides in several real samples including orange, pear, grape, peaches and agricultural water.

Kinetics of Guest-Induced Structural Transitions in Metal–Organic-Framework MIL-53(Al)-NH2 Probed by High-Pressure Nuclear Magnetic Resonance

A nuclear magnetic resonance (NMR) study of a pore opening in amino-functionalized metal-organic framework (MOF) MIL-53(Al) in response to methane pressure variation is presented. Variations of both NMR signal intensities and transversal relaxation rates for methane are found to reveal hysteretic structural transitions in the MOF material, which are smeared out over broad pressure ranges. Experiments with pressure reversals upon an incomplete adsorption/desorption gave deeper insight into the microscopic transition mechanisms. These experiments have unequivocally proven that the non-stepwise pore opening/closing transitions observed in the experiments are governed by a distribution of the opening/closing pressures over different MOF crystallites, for example, due to a distribution of the crystal sizes or shapes. The slow kinetics of the structural transitions measured in the hysteresis regime revealed a complex free energy landscape for the phase transition process.

Efficient capture and storage of ammonia in robust aluminium-based metal-organic frameworks

The development of stable sorbent materials to deliver reversible adsorption of ammonia (NH3) is a challenging task. Here, we report the efficient capture and storage of NH3 in a series of robust microporous aluminium-based metal-organic framework materials, namely MIL-160, CAU-10-H, Al-fum, and MIL-53(Al). In particular, MIL-160 shows high uptakes of NH3 of 4.8 and 12.8 mmol g−1 at both low and high pressure (0.001 and 1.0 bar, respectively) at 298 K. The combination of in situ neutron powder diffraction, synchrotron infrared micro-spectroscopy and solid-state nuclear magnetic resonance spectroscopy reveals the preferred adsorption domains of NH3 molecules in MIL-160, with H/D site-exchange between the host and guest and an unusual distortion of the local structure of [AlO6] moieties being observed. Dynamic breakthrough experiments confirm the excellent ability of MIL-160 to capture of NH3 with a dynamic uptake of 4.2 mmol g−1 at 1000 ppm. The combination of high porosity, pore aperture size and multiple binding sites promotes the significant binding affinity and capacity for NH3, which makes it a promising candidate for practical applications.

Enhanced Solid-State Fluorescence of Flavin Derivatives by Incorporation in the Metal-Organic Frameworks MIL-53(Al) and MOF-5.

The flavin derivatives 10-methyl-isoalloxazine (MIA) and 6-fluoro-10-methyl-isoalloxazine (6F-MIA) were incorporated in two alternative metal-organic frameworks, (MOFs) MIL-53(Al) and MOF-5. We used a post-synthetic, diffusion-based incorporation into microcrystalline MIL-53 powders with one-dimensional (1D) pores and an in-situ approach during the synthesis of MOF-5 with its 3D channel network. The maximum amount of flavin dye incorporation is 3.9 wt% for MIA@MIL-53(Al) and 1.5 wt% for 6F-MIA@MIL-53(Al), 0.85 wt% for MIA@MOF-5 and 5.2 wt% for 6F-MIA@MOF-5. For the high incorporation yields the probability to have more than one dye molecule in a pore volume is significant. As compared to the flavins in solution, the fluorescence spectrum of these flavin@MOF composites is broadened at the bathocromic side especially for MIA. Time-resolved spectroscopy showed that multi-exponential fluorescence lifetimes were needed to describe the decays. The fluorescence-weighted lifetime of flavin@MOF of 4 ± 1 ns also corresponds to those in solution but is significantly prolonged compared to the solid flavin dyes with less than 1 ns, thereby confirming the concept of "solid solutions" for dye@MOF composites. The fluorescence quantum yield (ΦF) of the flavin@MOF composites is about half of the solution but is significantly higher compared to the solid flavin dyes. Both the fluorescence lifetime and quantum yield of flavin@MOF decrease with the flavin loading in MIL-53 due to the formation of various J-aggregates. Theoretical calculations using plane-wave and QM/MM methods are in good correspondence with the experimental results and explain the electronic structures as well as the photophysical properties of crystalline MIA and the flavin@MOF composites. In the solid flavins, π-stacking interactions of the molecules lead to a charge transfer state with low oscillator strength resulting in aggregation-caused quenching (ACQ) with low lifetimes and quantum yields. In the MOF pores, single flavin molecules represent a major population and the computed MIA@MOF structures do not find π-stacking interactions with the pore walls but only weak van-der-Waals contacts which reasons the enhanced fluorescence lifetime and quantum yield of the flavins in the composites compared to their neat solid state. To analyze the orientation of flavins in MOFs, we measured fluorescence anisotropy images of single flavin@MOF-5 crystals and a static ensemble flavin@MIL53 microcrystals, respectively. Based on image information, anisotropy distributions and overall curve of the time-resolved anisotropy curves combined with theoretical calculations, we can prove that all fluorescent flavins species have a defined and rather homogeneous orientation in the MOF framework. In MIL-53, the transition dipole moments of flavins are orientated along the 1D channel axis, whereas in MOF-5 we resolved an average orientation that is tilted with respect to the cubic crystal lattice. Notably, the more hydrophobic 6F-MIA exhibits a higher degree order than MIA. The flexible MOF MIL-53(Al) was optimized essentially to the experimental large-pore form in the guest-free state with QuantumEspresso (QE) and with MIA molecules in the pores the structure contracted to close to the experimental narrow-pore form which was also confirmed by PXRD. In summary, the incorporation of flavins in MOFs yields solid-state materials with enhanced rigidity, stabilized conformation, defined orientation and reduced aggregations of the flavins, leading to increased fluorescence lifetime and quantum yield as controllable photo-luminescent and photo-physical properties.

Breathing Behaviour Modification of Gallium MIL-53 Metal-Organic Frameworks Induced by the Bridging Framework Inorganic Anion.

Controlling aspects of the μ2 -X- bridging anion in the metal-organic framework Ga-MIL-53 [GaX(bdc)] (X- =(OH)- or F- , bdc=1, 4-benzenedicarboxylate) is shown to direct the temperature at which thermally induced breathing transitions of this framework occur. In situ single crystal X-ray diffraction studies reveal that substituting 20 % of (OH)- in [Ga(OH)(bdc)] (1) for F- to produce [Ga(OH)0.8 F0.2 (bdc)] (2) stabilises the large pore (lp) form relative to the narrow pore (np) form, causing a well-defined decrease in the onset of the lp to np transition at higher temperatures, and the adsorption/desorption of nitrogen at lower temperatures through np to lp to intermediate (int) pore transitions. These in situ diffraction studies have also yielded a more plausible crystal structure of the int-[GaX(bdc)] ⋅ H2 O phases and shown that increasing the heating rate to a flash heating regime can enable the int-[GaX(bdc)] ⋅ H2 O to lp-[GaX(bdc)] transition to occur at a lower temperature than np-[GaX(bdc)] via an unreported pathway.

A LDH Template Triggers the Formation of a Highly Compact MIL-53 Metal-Organic Framework Membrane for Acid Upgrading.

Highly compact metal-organic framework (MOF) membranes offer hope for the ambition to cope with challenging separation scenarios with industrial implications. A continuous layer of layered double hydroxide (LDH) nanoflakes on an alumina support as a template triggered a chemical self-conversion to a MIL-53 membrane, with approximately 8 hexagonal lattices (LDH) traded for 1 orthorhombic lattice (MIL-53). With the sacrifice of the template, the availability of Al nutrients from the alumina support was dynamically regulated, which resulted in synergy for producing membranes with highly compact architecture. The membrane can realize nearly complete dewatering from formic acid and acetic acid solutions, respectively, and maintain stability in a continuous pervaporation over 200 h. This is the first success in directly applying a pure MOF membrane to such a corrosive chemical environment (lowest pH value of 0.81). The energy consumption is saved by up to 77 % when compared with the traditional distillation.

Preparation of heterogeneous catalysts by the post-synthetic modification of mesoporous metal-organic framework MIL-101

Preparation of heterogeneous catalysts by the post-synthetic modification of mesoporous metal-organic framework MIL-101

Constructing Defective Heterojunctions of BiOBr Nanosheets and Hollow NH2 -functionalized MOFs for Visible-light-driven CO2 Reduction with Nearly 100% CO Selectivity by Pure H2 O.

To rationally design photocatalysts with high generation rate and selectivity of target product remains an ongoing challenge for CO2 conversion in pure H2 O. Herein, from the viewpoint of enhancing the separation efficiency of photoinduced electron-hole pairs and the adsorption ability of CO2 molecule, we have constructed a series of Z-scheme defective heterojunctions of BiOBr nanosheets and hollow NH2 -functionalized metal-organic framework (MOF) MIL-125 with Ti ions as metal centers (noted as NH2 -MIL-125(Ti)). Systematic characterization demonstrates that the BiOBr nanosheets are anchored on the surface of hollow NH2 -MIL-125(Ti), which facilitates the efficient visible-light-driven catalytic reduction of CO2 to CO with nearly 100% selectivity by pure H2 O. Especially, the CO generation rate of optimized catalyst with oxygen vacancies reaches 459.7 μmol g-1 h-1 , which is higher than those of all the previously reported photocatalysts without sacrificial reagents. This approach provides a new insight for using inorganic semiconductors to fabricate semiconducting MOFs for high-efficiency photocatalytic reduction CO2 into value-added chemicals by pure H2 O.

Isolation of Cytochrome C for Proteomics with Lindqvist-type Polyiodate Modified Metal Organic Framework

Separation and purification of Cytochrome C (Cyt-C) is important for proteomic. High efficient and selective pretreatment method for Cyt-C in real samples are always needed. Herein, polyniobate (K7H[Nb6O19]·13H2O, Nb6O19) is modified on a metal-organic framework MIL-125(Ti) through intermolecular hydrogen bonds and an aqueous-stable composite Nb6O19/MIL-125(Ti) is successfully prepared to pretreat complex protein sample. Protein adsorption studies have shown that Nb6O19/MIL-125(Ti) can promote the selective adsorption of Cyt-C due to the synergistic effect of electrostatic and hydrogen-bond interactions. At pH=10.0 (Britton-Robinson buffer), the adsorption efficiency of 300 μL 100 μg·mL−1 Cyt-C onto 1.0 mg Nb6O19/MIL-125(Ti) can reach 99.5%. The adsorption behavior of Cyt-C fits well with the Langmuir adsorption model, corresponding to a maximum theoretical adsorption capacity of 168.35 mg·g−1. Using 3 mol·L−1 NaCl as the eluent, a high elution efficiency of 92.19% is obtained. In addition, the results of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis confirm that Nb6O19/MIL-125(Ti) efficiently adsorbed Cyt-C from scrofa heart extraction. LC-MS/MS spectrometry results show that the purification of Cyt-C reduces the abundance from the 12th to the 154th place after Nb6O19/MIL-125(Ti) treatment. Moreover, low abundant proteins, e.g., Superoxide dismutase 1, IF rod domain-containing protein and Ubiquitin-60S ribosomal protein L40 were considerably enriched. These outcomes confirm the practicability of Nb6O19/MIL-125 (Ti) as a Cyt-C extractant has potential application value in scrofa heart proteomics.