This research is carried out to study the effect of ascending and descending effort on some biochemical parameters related to heart function which include: Arterial natriuretic hormone, angiotensin II, sodium, potassium, lactate dehydrogenase, creatine kinase, aspartate aminotransferase, adiponectin, lipase, triglycerides, free fatty acid, lipooxygenase and myeloperoxidase . The major samples included (15) of the track and field players participating in Mosul university team of middle-distance runners .
 The results showed a significant increase for ascending directly post-effort test compared to pre-effort test in: sodium, lactate dehydrogenase, triglycerides and myeloperoxidase and significant decrease in: aspartate aminotransferase, lipase and lipooxygenase.
 The results revealed significant increase for descending post-effort directly test compared with pre-effort test in the levels of sodium, potassium, lactate dehydrogenase and triglycerides significant decrease in: lipase, free fatty acids and lipooxygenase.
 Moreover the results, showed significant increase for ascending effort test compared with descending effort test in levels: potassium and aspartate aminotransferase and significant decrease for myeloperoxidase .
 The study concluded the need to give the current examination of physical effort ascending and descending for the early detection of heart patients.