Background and Study Aim. Several mineral elements are necessary for the athlete's body, which significantly impact the development of sports achievements because of their active role in bringing internal balance to the athlete's body. The study aims to determine the concentration of copper in the blood before and after the implementation of physical exertion at a level of 180 pulse/min on middle-distance runners.
 Material and Methods. Twelve athletes who are team members at Egyptian clubs were chosen deliberately as a research sample. The study participants were at the end of their special preparation. The experiment was conducted between July 12, 2021, and July 14, 2021, on the research sample, resting after exerting physical effort by running on a treadmill at a speed of 10 km / h. The runner continued at this speed for 3 minutes, after which the device's speed increased by 2 km / h every minute until reaching a heart rate of 180 pulse/ min.
 Results. The mineral differences in the copper element are attributed to the immediate induced by physical exertion by increasing the copper element in the blood within the normal level. This causes many changes and responses within the body cells. The copper element plays a significant role in oxidation and reduction processes via enzymes that require the copper element to produce energy in the cells and tissues due to the body's exposure to physical exertion. Athletes' exposure to physical exertion causes copper-dependent enzymes to produce energy in cells and tissues due to the element's requirement.
 Conclusions. Copper affected the physical effort of middle-distance runners at a heart rate of 180 pulse/min. In addition, Middle-distance runners' blood pressure (systolic and diastolic) was affected by the physical exertion at a heart rate of 180 rpm.