Mid Stages Research Articles

A novel role of Tinospora cordifolia in amelioration of cancer-induced systemic deterioration by taming neutrophil infiltration and hyperactivation

BackgroundCancer has emerged as a systemic disease which targets various organs thus challenging the overall physiology of the host. Recently, we have shown that hyperactive neutrophils infiltrate various organs of tumor bearing host and contribute to gradual systemic deterioration. Therefore, taming neutrophils via potent immunomodulators could be an appropriate therapeutic approach in regulating systemic damage. Tinospora cordifolia (TC), an Ayurvedic panacea, is known for its immense medicinal values in traditional literature and recent reports have also documented its immunomodulatory potential. However, whether TC can regulate neutrophils to exert its therapeutic effectiveness has not been deciphered so far. MethodsFor the in vivo study, we utilized murine model of Dalton's Lymphoma (DL). T. cordifolia extract (TCE) treatment was scheduled at early, mid and advanced stages of tumor growth at a dose of 400 mg/kg b.w for 30 consecutive days. Effect of TCE on neutrophil infiltration was examined by immunostaining. Neutrophil elastase (NE) level in serum, ascitic fluid and various tissues was monitored by ELISA. Further, qPCR was performed to assess transcripts levels of NE, myeloperoxidase (MPO), metalloproteinases (MMP-8, MMP-9) and cathepsin G (CSTG) in various tissues. ROS level in tissue was assessed by DHE staining and organ function was assessed by histology post TCE treatment. ResultsOur findings showed that TC treatment significantly reduced neutrophil count in peripheral blood and their infiltration in vital organs of tumor-bearing host. Further, it ameliorated neutrophil hyperactivation by down regulating the expression of its key cargoes including NE, MPO, MMP-8, MMP-9 and CSTG at early and mid stage of tumor growth. In addition, TC treatment prevented histopathological alterations and restored the normal serum enzyme levels at different stages of tumor growth. Importantly, TC treatment also showed significant reduction in tumor burden which was accompanied by a remarkable increase in survival of the tumor-bearing mice. ConclusionsWe conclude that T. cordifolia could limit systemic damage via regulating neutrophil infiltration and hyperactivation which can further lead to cancer control at both prophylactic and therapeutic level.

Environmental and Economic Impacts of Biodegradable Plastic Film Mulching on Rainfed Maize: Evaluations on Sustainability and Productivity

Fully biodegradable (Bio) plastic film is an alternative option to replace widely used polythene film in semiarid rainfed regions. However, its productivity and environmental friendliness remain unclear. Field observations were conducted using maize variety Pioneer 335 to evaluate the effects of Bio film mulching on soil hydrothermal status, carbon sequestration, and water productivity in a semiarid site of northwest China from 2016 to 2017. Six treatments were designed as (1) CK-1, ridge and furrow (RF) without mulching, (2) CK-2, conventional flat planting without mulching, (3) RFT, RF with transparent polyethylene film mulching, (4) RFB, RF with black polyethylene mulching, (5) RFS, RF with wheat straw mulching, and (6) RFBIO, RF with Bio film mulching. The results indicated the growth prophase of maize from sowing to silking stage received 160 mm of rainfall in cool and wet 2016, but decreased to 119.8 mm in warm and dry 2017. Bio film degradation was advanced at the mid stages of maize growth by 10 days in 2017 compared with 2016. Similarly to RFT, RFBIO significantly improved soil hydrothermal conditions compared with RFS, CK-1, and CK-2; however, its magnitude decreased at the maturity stage (P < 0.05). Both RFT and RFB had significantly higher grain yields, economic benefits and water use efficiencies than RFBIO and RFS did across two growing seasons (P < 0.05). RFBIO led to a steady improvement in soil organic carbon, light fraction organic carbon, and carbon to nitrogen ratio, which were better than those of RFT. Therefore, Bio film mulching might be environmentally friendly but not highly productive.

RNA-Seq transcriptomic analysis reveals gene expression profiles of acetic acid bacteria under high-acidity submerged industrial fermentation process.

Acetic acid bacteria (AAB) are Gram-negative obligate aerobics in Acetobacteraceae family. Producing acetic acid and brewing vinegars are one of the most important industrial applications of AAB, attributed to their outstanding ability to tolerate the corresponding stresses. Several unique acid resistance (AR) mechanisms in AAB have been revealed previously. However, their overall AR strategies are still less-comprehensively clarified. Consequently, omics analysis was widely performed for a better understanding of this field. Among them, transcriptome has recently obtained more and more attention. However, most currently reported transcriptomic studies were conducted under lab conditions and even in low-acidity environment, which may be unable to completely reflect the conditions that AAB confront under industrialized vinegar-brewing processes. In this study, we performed an RNA-Seq transcriptomic analysis concerning AAB’s AR mechanisms during a continuous and periodical industrial submerged vinegar fermentation process, where a single AAB strain performed the fermentation and the acetic acid concentration fluctuated between ~8% and ~12%, the highest acidity as far we know for transcriptomic studies. Samples were directly taken from the initial (CK), mid, and final stages of the same period of the on-going fermentation. 16S rRNA sequence analysis indicated the participation of Komagataeibacter europaeus in the fermentation. Transcriptomic results demonstrated that more genes were downregulated than upregulated at both mid and final stages. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrich analysis reflected that the upregulated genes mainly carried out tricarboxylic acid cycle and oxidative phosphorylation processes, probably implying a considerable role of acetic acid overoxidation in AR during fermentation. Besides, upregulation of riboflavin biosynthesis pathway and two NAD+-dependent succinate-semialdehyde dehydrogenase-coding genes suggested a critical role of succinate oxidation in AR. Meanwhile, downregulated genes were mainly ribosomal protein-coding ones, reflecting that the adverse impact on ribosomes initiates at the transcription level. However, it is ambiguous whether the downregulation is good for stress responding or it actually reflects the stress. Furthermore, we also assumed that the fermentation stages may have a greater effect on gene expression than acidity. Additionally, it is possible that some physiological alterations would affect the AR to a larger extent than changes in gene expression, which suggests the combination of molecular biology and physiology research will provide deeper insight into the AR mechanisms in AAB.

Effects of acupotomy on partial movement gait and serum tumor necrosis factor-α, interleukin-1β in patients with knee osteoarthritis

To explore effects of acupotomy on pain, function, gait and serum inflammatory factors in patients with knee osteoarthritis(KOA). From December 2017 to June 2019, 110 patients with KOA were collected and divided into acupotomy group(56 cases) and western medicine group(54 cases) by using random number table method. In acupotomy group, there were 16 males and 40 females, aged from 46 to 74 years old with an average of (62.98±6.68) years old, the course of disease ranged from 1 to 240 months with an average of 24.5(15.25, 33.00) months;were treated with acupotomy on the pain points around knee joint once a week for 3 weeks. In western medicine group, there were 18 males and 36 females, aged from 47 to 73 years old with an average of (64.19±5.98 ) years old;the course of disease ranged from 1 to 220 months with an average of 25.00(13.75, 33.00) months;were took celecoxib capsule orally, 200 mg once a day for 3 weeks. Oxford Knee Score(OKS) was performed before treatment, 3 weeks and 3 months after treatment. Gait kinematics analysis and serum levels of tumor necrosis factor-α(TNF-α) and interleukin-1β (IL-1β) were measured before and after treatment for 3 weeks. All patients were followed up from 6 to 24 months with an average of(15.03±4.55) months. OKS between two groups decreased significantly at 3 weeks and 3 months after treatment(P<0.001). Functional scores and overall scores in acupotomology group were significantly decreased at 3 months compared with 3 weeks after treatment(P<0.001). OKS of acupotomy group were significantly lower than those of western medicine group at 3 weeks and 3 months after treatment(P<0.05). Gait speed, frequency and length between two groups were significantly improved at 3 weeks after treatment(P<0.05). At 3 weeks after treatment, gait freguency of acupotomy group was significantly improved compared with western medicine group(P<0.05). TNF-α and IL-1β were significantly lower in both groups at 3 weeks after treatment than before treatment(P<0.05). At 3 weeks after treatment, level of IL-1 β was lower in western medicine group than in acupotomy group(P<0.05), and difference in TNF-α level was not statistically significant(P>0.05). Acupotomology of pain points could significantly improve pain, function, gait, and decreased serum inflammatory factors at early to mid stage of KOA patients, in particular, it is superior to non-steroidal anti-inflammatory drugs in terms of knee function recovery and cadence improvement.

Effects of feed transition on digestive tract digestive enzyme, morphology and intestinal community in cuttlefish (Sepia pharaonis)

Sepia pharaonis is an excellent candidate for aquaculture in China. However, the low survival rate during early feed transition is a bottleneck restricting industrial development. Understanding the changes in digestive physiology and intestinal microflora during feed transition should enable us to meet their nutritional needs to improve production. In this study, we investigate the digestive enzyme of S. pharaonis and undertake histological observations of the digestive gland and intestine. The intestinal microflora 16S rRNA genes were also analyzed using high-throughput sequencing of the pre, mid, and post-feed transition stages (20, 40, and 60 days post-hatching (DPH), respectively). The digestive enzymes from the digestive gland (trypsin and chymotrypsin) rapidly decrease at 40 DPH when compared to their levels at 20 DPH, but mostly recovered by 60 DPH. The alkaline phosphatase and lipase increased sharply by 40 DPH, then peaked at 60 DPH. The intestinal digestive enzymes followed similar trends during feed transition, except for lipase activity, which decreased after 20 DPH and remained low, even at 60 DPH. Feed transition affects the morphogenesis of the digestive tract and feed transition stress leads to the impairment of the digestive gland and intestinal morphology, which reduces the digestive capacity, but almost totally recovers by 60 DPH. Moreover, the comparison of the intestinal microbial composition during feed transition revealed that the dominant phylum Bacteroidetes gradually increased to a peak at 40 DPH and then decreased until 60 DPH. The microbial composition changed with the most abundant genus Pseudomonas being replaced by Acinetobacter. The phylum and family level investigation suggested the microbiota in the rearing water had limited influence on the intestinal microbiota. The intestinal microbiota diversity increased during feed transition. This study improves our understanding of changes and adaptations in cuttlefish during feed transition.

Family Care-Work Interference and Mid/Late-Career Motivation and Intentions: Mediating Role of Occupational Future Time Perspective

The combination of work and family obligations can lead to two-way interference, resulting in Family Care Interference with Work (FCIW) and Work Interference with Family Care (WIFC). Both can impact late career motivation and intentions to continue working or retire and reduce their occupational time perspective (OFTP). Through two studies, this paper shows how OFTP mediates the relationship between FCIW/WIFC and mid and late career indicators such as early retirement intentions and motivation to continue working beyond the retirement age. Our findings contribute to increasing knowledge of how increasing family caregiving obligations may impact the career intentions of working carers in their mid and late career stages.

Sex steroid profile during oocyte development and maturation in the intertidal worm Marphysa madrasi (Polychaeta: Eunicidae) from the east coast of India

Marphysa madrasi is a commercially valuable maturation diet in crustacean aquaculture. This study presents the first detailed investigation of oogenesis in the intertidal polychaete worm M. madrasi and reports the steroid profile during oocyte growth and development. Oogenesis is extraovarian type I, originating from coelomic epithelial cells, with four stages of development - primary growth, early vitellogenic, late vitellogenic, and maturation. The primary growth phase contains oogonial cells and previtellogenic oocyte clusters in the early, mid, and late stages of development form a dispersed ovary attached to blood vessels. The late previtellogenic oocytes detach from the ovary at the onset of vitellogenesis. The detached oocytes complete vitellogenesis and final maturation in the coelomic fluid as solitary free-floating cells without any connection with follicle cells. The worms display asynchronous reproduction with a heterogeneous population of developing oocytes. Steroid extracts from the polychaete homogenates in different stages of oogenesis were identified by HPLC and confirmed by LC-MS/MS. In M. madrasi, two vertebrate-type steroids, pregnenolone (P5) and 17α-hydroxyprogesterone (17-OHP) were detected and quantified. The P5 levels were low in immature worms but increased significantly by ∼ 8.3-fold in the previtellogenic stage and peaked during oocyte maturation. 17-OHP levels were low in immature worms but gradually increase as the oogenesis progress to the primary growth and early vitellogenic phase, with a significant increase (p < 0.001) during the late vitellogenic phase. Although an increase in the concentration of P5 and 17-OHP during vitellogenesis and maturation of oocytes points to a possible role in reproduction, the absence of other vertebrate-type steroids in the investigated polychaete signifies a plausible uptake of P5 and 17-OHP from the environment.

Transcriptome analysis of female western flower thrips, Frankliniella occidentalis, exhibiting neo-panoistic ovarian development.

The western flower thrips, Frankliniella occidentalis, is one of the most devastating insect pests with explosive reproductive potential. However, its reproductive physiological processes are not well understood. This study reports the ovarian development and associated transcriptomes of F. occidentalis. Each ovary consisted of four ovarioles, each of which contained a maximum of nine follicles in the vitellarium. The germarium consisted of several dividing cells forming a germ cell cluster, presumably consisting of oocytes and nurse cells. The nurse cells were restricted to the germarium while the subsequent follicles did not possess nurse cells or a nutritive cord, supporting the neo-panoistic ovariole usually found in thysanopteran insects. Oocyte development was completed 72 h after adult emergence (AAE). Transcriptome analysis was performed at mid (36 h AAE) and late (60 h AAE) ovarian developmental stages using RNA sequencing (RNASeq) technology. More than 120 million reads per replication were matched to ≈ 15,000 F. occidentalis genes. Almost 500 genes were differentially expressed at each of the mid and late ovarian developmental stages. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these differentially expressed genes (DEGs) were associated with metabolic pathways along with protein and nucleic acid biosynthesis. In both ovarian developmental stages, vitellogenin, mucin, and chorion genes were highly (> 8-fold) expressed. Endocrine signals associated with ovarian development were further investigated from the DEGs. Insulin and juvenile hormone signals were upregulated only at 36 h AAE, whereas the ecdysteroid signal was highly maintained at 60 h AAE. This study reports the transcriptome associated with the ovarian development of F. occidentalis, which possesses a neo-panoistic ovariole.

Risk factors for mid- and late-feeding-stage bovine respiratory morbidity and mortality based on individual animal treatments of beef feedlot cattle

Previously published research on bovine respiratory disease (BRD) has emphasized the initial portion of the feeding phase; yet, little work has investigated risk factors associated with BRD incidence later in the feeding period. Our study objective was to identify risk factors associated with BRD morbidity and mortality timing within the feeding phase. Individual animal records for first BRD treatment (n = 188,437) or BRD mortality (n = 13,991) were classified as early stage, middle stage, or late stage based on the percentage of their cohorts’ feeding phase that was complete at day of event (event days on feed/cohort total days on feed). There were 141,097 early stage, 33,871 middle stage, and 13,469 late stage at first treatments for BRD and 7,821 early stage, 3,625 middle stage, and 2,545 late stage at death. Two ordinal logistic regression models (morbidity, mortality) were used to evaluate the association of cattle demographic factors (arrival weight, arrival lot size, sex, metaphylaxis, quarter of arrival) and the probability of an individual animal first treatment for BRD or mortality from BRD being early, middle, or late stage. All 2-way interactions of cattle demographics for morbidity were significant (P < 0.05), whereas for mortality, main effects of arrival weight and metaphylaxis were significant (P < 0.05) and only the 2-way interactions of sex by quarter of arrival and sex by lot size were significant (P < 0.05). In general, heifers, heavier animals at arrival, and cattle that arrived at the yard in the first and second quarter were the most likely demographic categories to have an initial diagnosis of BRD in the mid or late stage of the feeding phase.

Glacial biodiversity of the southernmost glaciers of the European Alps (Clapier and Peirabroc, Italy)

We applied a multi-taxa approach integrating the co-occurrence of plants, ground beetles, spiders and springtails with soil parameters (temperatures and chemical characteristics) in order to describe the primary succession along two glacier forelands in the Maritime Alps (Italy), a hotspot of Mediterranean biodiversity. We compared these successions to those from Central Alps: Maritime glacier forelands markedly differ for their higher values of species richness and species turnover. Contrary to our expectation, Maritime glacier forelands follow a ‘replacement change model’, like continental succession of Inner Alps and differently from other peripheral successions. We propose that the temperatures along these Mediterranean glacier forelands are warmer than those along other Alpine glacier forelands, which promote the faster species turnover. Furthermore, we found that early and mid successional stages of the investigated glaciers are richer in cold-adapted and endemic species than the later ones: we confirmed that the ‘replacement change’ model disadvantages pioneer, cold-adapted species. Given the overall correspondence among cold-adapted and endemic species, the most threatened in this climate phase, our results raise new concerns about the extinction risk of these species. We also describe supraglacial habitat of Maritime glaciers demonstrating that supraglacial debris represents an environment decoupled from the regional climate and may have an important role as refugium for coldadapted and hygrophilous plant and animal species, whose survival can be threatened by climate change and by a rapid ecological succession in the adjacent forelands.

Herbage and Silage Quality Improved More by Mixing Barley and Faba Bean Than by N Fertilization or Stage of Harvest

Legume–cereal mixtures are pivotal in yielding a more balanced forage composition compared to the sole crops, due to the well-adjusted equilibrium of carbohydrate and protein. However, great attention is required in choosing the optimal ratio of the components for the mixtures and other agronomic practices (including N fertilization and stage of harvest), because they can sharply change the forage composition and quality. To fill this gap, the present research compared the herbage and silage qualities and key fermentative and nutritional traits of biomasses obtained by: (i) five mixtures (i.e., 100:0; 75:25, 50:50, 25:75, and 0:100) of hybrid barley (Hordeum vulgare L.) and faba bean (Vicia faba var. minor); (ii) two N barley fertilization rates (0 vs. 140 kg N ha−1); and (iii) three stages of harvest (milky, early dough and soft dough). We found that the presence of faba bean improved the forage quality, either herbage or silage, through different mechanisms. In the herbage mixtures, faba bean inclusion increased crude protein (CP), and, when compared to the fertilized barley, also water-soluble sugar (WSCs) concentration, with small effects on the relative feed values (RFV) and total digestible nutrients (TDN). In the silage, the higher the faba bean share in the mixture, the higher the RFV, the non-fibrous carbohydrates, and total and lactic acid concentrations, and the lower the pH and the hemicellulose and cellulose concentrations, particularly at the early and mid stages of harvest. These improvements were likely due to the high ability of faba bean to attain a vegetative status (i.e., low dry matter concentration) by the end of the cropping season, and to improve the organic acid production. Our results suggest that the inclusion of faba bean in a barley-based silage system can widen the window for silage harvesting, thanks to its ability to sustain the fermentation process, despite a lower WSC to CP ratio compared to the unfertilized barley. These results occurred almost irrespectively of the faba bean share in the mixture and barley fertilization. This implies that when designing intercrop mixtures, the effect of crop densities on yield should be considered more than on quality.

A STUDY ON SERUM PROTEIN PROFILE AT DIFFERENT LACTATION STAGES IN UPGRADED MURRAH BUFFALOES

The aim of the present study was to find out the serum protein profile of upgraded Murrahbuffaloes in different lactation stages. The blood samples were collected from 12 healthylactating upgradedMurrah buffaloes in early (0-13 weeks), mid (14-26 weeks) and late (27-40 weeks) lactation stages andseparated serum was analyzed for total protein, albumin, globulin, albumin: globulin ratio, urea, uric acidand creatinine. All the constituents under study differed significantly within the respective means amongstthe three stages of lactation with exception of albumin:globulin ratio. The values of total serum protein,globulin and uric acid were decreased significantly throughout the lactation period while urea andcreatinine increased significantly with progress of lactation. Serum albumin was decreased from early tomid stage then increased from mid to late lactation.

Effects of Camellia japonica Roots Extract on Myoblast Differentiation and Muscle Atrophy in Association with Mitochondrial Function

ObjectivesSkeletal muscle atrophy is caused by various diseases and conditions including aging, cancer, and cancer treatments. Chemotherapy-induced muscle atrophy leads to decrease quality of life and increase morbidity and mortality. Recent evidence suggests oxidative stress is a cause and/or consequence of mitochondrial dysfunction, which is strongly associated with muscle atrophy. Given Camellia japonica roots extract (CJT) has antioxidant property, we investigated whether CJT would ameliorate myoblast differentiation and chemotherapy-induced muscle atrophy in vitro and in vivo to provide evidence for candidates to prevent and treat muscle atrophy. MethodsC2C12 myoblasts were differentiated in the presence of CJT (0.1, 1, 10, and 100 ng/mL) and cells were collected to perform myosin heavy chain (MHC) immunostaining and qRT-PCR for the measurement of markers related with myogenesis and mitochondrial function. Wild type AB * zebrafish (Danio rerio) embryos were treated with FOLFIRI (5-FU, LV, and CPT-11) and CJT, and immunostained to detect slow and fast types of muscle. The Tg(Xla.Eef1a1: mlsEGFP) zebrafish line expressing mitochondria-targeted green fluorescent protein (mito-GFP) was used to monitor mitochondrial morphology. ResultsCJT increased the formation of MHC-positive multinucleated myotubes (≥5 nuclei) in a dose-dependent manner, while it decreased the number of mononuclear myotubes. CJT significantly enhanced the expression of myogenic differentiation markers including Myod (early stage), Myog (mid stage), and MHC isoforms (late stage) including Myh 1, Myh 2, Myh 4, and Myh7. Mitochondrial biogenesis markers such as SDHA (Complex Ⅱ) and COX1 (Complex IV) were significantly increased at 100 ng/mL of CJT than control. CJT tended to increase the expression of MFN2 and NRF1 involved in mitochondrial fusion and biogenesis, respectively. In addition, CJT alleviated FOLFIRI-induced muscle atrophy both in slow and fast muscle fibers in zebrafish embryos. Similarly, CJT improved FOLFIRI-induced mitochondrial dysfunction in a dose-dependent manner. ConclusionsOur data indicates that CJT promotes myogenesis and alleviates muscle atrophy in association with mitochondrial function, suggesting CJT has potential as a nutritional supplement for the prevention and treatment of muscle atrophy. Funding SourcesNRF (2020R1C1C1007553 to S-EK)

Maternal diabetes independent of BMI is associated with altered accretion of adipose tissue in large for gestational age fetuses.

AimTo analyse the effects of maternal diabetes mellitus (DM) and body mass Index (BMI) on central and peripheral fat accretion of large for gestational age (LGA) offspring.MethodsThis retrospective study included LGA fetuses (n = 595) with ultrasound scans at early (19.23 ± 0.68 weeks), mid (28.98 ± 1.62 weeks) and late (36.20 ± 1.59 weeks) stages of adipogenesis and measured abdominal (AFT) and mid-thigh (TFT) fat as surrogates for central and peripheral adiposity. Women were categorised according to BMI and DM status [pre-gestational (P-DM; n = 59), insulin managed (I-GDM; n = 132) and diet managed gestational diabetes (D-GDM; n = 29)]. Analysis of variance and linear regressions were applied.ResultsAFT and TFT did not differ significantly between BMI categories (normal, overweight and obese). In contrast, AFT was significantly higher in pregnancies affected by D-GDM compared to non-DM pregnancies from mid stage (0.44 mm difference, p = 0.002) and for all DM categories in late stage of adipogenesis (≥ 0.49 mm difference, p < 0.008). Late stage TFT accretion was higher than controls for P-DM and I-GDM but not for D-GDM (0.67 mm difference, p < 0.001; 0.49 mm difference, p = 0.001, 0.56 mm difference, p = 0.22 respectively). In comparison to the early non-DM group with an AFT to TFT ratio of 1.07, the I-GDM group ratio was 1.25 (p < 0.001), which normalised by 28 weeks becoming similar to control ratios.ConclusionsDM, independent of BMI, was associated with higher abdominal and mid-thigh fat accretion in fetuses. Use of insulin improved central to peripheral fat ratios in fetuses of GDM mothers.

Estimation of Landsat-like daily evapotranspiration for crop water consumption monitoring using TSEB model and data fusion.

Evapotranspiration (ET) plays an essential role in agricultural water resource management. Understanding regional agricultural water consumption characteristics can be improved by predicting ET using remote sensing. However, due to the lack of high-resolution images on clear-sky days or the limitation of ET reconstruction on cloudy-sky days, it remains challenging to continuously derive ET at the field scale. In this study, the Landsat and MODIS data were initially fused to obtain the Landsat-like vegetation index and land surface temperature on clear-sky days. Then the two-source energy balance (TSEB) model was applied to calculate the daily ET during the clear-sky. A canopy resistance-based gap-filling method was involved in reconstructing regional ET on cloudy days while considering different environmental factors. The estimations were validated by automatic weather system data (AWS) and eddy covariance (EC) measurements in Guantao County. The results demonstrated that the proposed scheme performed well in estimating cropland ET, with an RMSE of 0.86 mm·d−1 and an R2 of 0.65, and the NSE and PBias were 0.61 and -0.29%, respectively. The crop water consumption analysis revealed that the daily ET of winter wheat peaked during the maturation stage. Nevertheless, summer maize water consumption peaked in the middle of the growing season in this area. The temperature during the early development stage and the soil moisture in the mid and late growth stages had the greatest impact on the ET of winter wheat. During the entire growing period, soil moisture had the largest effect on the ET of summer maize. The findings showed that the TSEB model can be effectively applied to field-scale water consumption monitoring in North China through MODIS and Landsat data fusion and ET temporal reconstruction considering environmental factors.

Secondary Succession Altered the Diversity and Co-Occurrence Networks of the Soil Bacterial Communities in Tropical Lowland Rainforests.

The characteristics of plant and soil bacterial communities in forest ecosystems have been reported, but our understanding of the relationship between plant communities and soil bacteria in different stages of secondary tropical rainforest succession is still poor. In June 2018, three different natural successional stages of tropical lowland rainforests, early (33 years), early-mid (60 years), and mid successional stage (73 years), in Hainan Island, China, were selected for this study. By conducting field investigation and 16S rRNA gene high-throughput sequencing, the composition and diversity of tree communities, the niche overlap of tree species with legumes among tree species, and the diversity and composition of soil bacterial communities and co-occurrence networks within communities across the successional stages were investigated. The results showed that plant richness and species diversity increased significantly during the secondary succession of tropical lowland rainforests. The order of positive correlations between nitrogen-fixing legumes and other species in plant communities was early-mid > mid > early successional stage. Soil nutrient content and soil bacterial richness were highest in the early-mid stages of succession, followed by mid and early stages of succession. Organic matter (OM), total nitrogen (TN), alkali nitrogen (AN), and available phosphorus (AP) had a stronger positive impact on soil bacterial communities. Co-occurrence network analysis showed that with the advancement of rainforests succession, the negative correlation between soil bacterial species decreased, and the community stability increased. Overall, as a result of tropical lowland rainforest secondary natural succession, the richness and diversity of plant communities increased, which altered the living conditions of nitrogen-fixing legumes and the soil properties, and the network complexity of soil bacterial communities increased with the rising of rainforest soil nutrient content.

Quantitative estimation of reclamation status of a dry secondary forest using “measure of climax”: A novel approach

Determination of reclamation status of secondary forests is particularly urgent in human impacted landscapes for conservation of biodiversity and other ecosystem services. However, there is a lack of a quantitative method to estimate the recovery status of secondary forest globally. In the present work, the authors have attempted to estimate the recovery status of a dry secondary forest of Similipal Biosphere Reserve (SBR), India quantitatively with a nearby primary forest. We have formulated an equation (measure of climax stage) to estimate the secondary forest for its possible climax stage (early climax stage/mid climax stage/stable climax stage/non climax stage) based on species richness. For this study, the value of “measure of climax stage” between 0 and 0.2 is considered as stable climax stage, 0.2–0.6 as mid climax stage and 0.6–1 as early climax stage. On the other hand, the value >1 was considered as a non-climax stage of the forest. Accordingly, the studied secondary forest was estimated as to be in mid climax stage (measure of climax stage = 0.4230) even after c. 25 years of successional stage. We have also estimated the recovery stage of a secondary dry forest in SBR by fitting it with a suitable equation and found that the time frame for the stable climax stage is more than 50 years. ANOVA analysis indicates that there is no significant difference observed in the case of soil nutrient contents (except Electrical Conductivity) between primary and secondary forest sites. This developed equation may be implicated in the evaluation of reclamation status of secondary and degraded forest lands globally. Further, in-depth study is encouraged for the development of a modern climax concept to understand the ecological restoration process in a better way.

Fledge or fail: Nest monitoring of endangered black-cockatoos using bioacoustics and open-source call recognition

Ecologists are increasingly using bioacoustics in wildlife monitoring programs. Remote autonomous sound recorders provide new options for collecting data for species and in contexts that were previously difficult. However, post-processing of sound files to extract relevant data remains a significant challenge. Detection algorithms, or call recognizers, can aid automation of species detection but their performance and reliability has been mixed. Further, building recognizers typically requires either costly commercial software or expert programming skills, both of which reduces their accessibility to ecologists responsible for monitoring. In this study we investigated the performance of open-source call recognizers provided by the monitoR package in R, a language popular among ecologists. We tested recognizers on sound data collected under natural conditions at nests of two endangered subspecies of black-cockatoo, the Kangaroo Island glossy black-cockatoo Calyptorhynchus lathami halmaturinus (n = 23 nests), and the south-eastern red-tailed black-cockatoo Calyptorhynchus banksii graptogyne (n = 20 nests). Specifically, we tested the performance of binary point matching recognizers in confirming daily nest activity (active or inactive) and nesting outcome (fledge or fail). We tested recognizers on recordings from nests of known status using 3 × 3-h recordings per nest, from early, mid and late stages of the recording period. Daily nest activity was correctly assigned in 61.7% of survey days analysed (n = 60 days) for the red-tailed black-cockatoo, and 62.3% of survey days (n = 69 days) for the glossy black-cockatoo. Fledging was successfully detected in all cases. Precision (true positive / true positive + false positive) of individual detections was 70.2% for the south-eastern red-tailed black-cockatoo and 37.1% for the Kangaroo Island glossy black-cockatoo. Manual verification of outputs is still required, but it is not necessary to verify all detections to confirm an active nest (i.e., nest is deemed active when true positives are identified). We conclude that bioacoustics combined with semi-automated post-processing can be an appropriate tool for nest monitoring in these endangered subspecies.

Postural control and gait measures derived from wearable inertial measurement unit devices in Huntington's disease: Recommendations for clinical outcomes

BackgroundPostural control impairments begin early in Huntington's disease yet measures most sensitive to progression have not been identified. The aims of this study were to: 1) evaluate postural control and gait in people with and without Huntington's disease using wearable sensors; and 2) identify measures related to diagnosis and clinical severity. Methods43 individuals with Huntington's disease and 15 age-matched peers performed standing with feet together and feet apart, sitting, and walking with wearable inertial sensors. One-way analysis of variance determined differences in measures of postural control and gait between early and mid-disease stage, and non-Huntington's disease peers. A random forest analysis identified feature importance for Huntington's disease diagnosis. Stepwise and ordinal regressions were used to determine predictors of clinical chorea and tandem walking scores respectively. FindingsThere was a significant main effect for all postural control and gait measures comparing early stage, mid stage and non-Huntington's disease peers, except for gait cycle duration and step duration. Total sway, root mean square and mean velocity during sitting, as well as gait speed had the greatest importance in classifying disease status. Stepwise regression showed that root mean square during standing with feet apart significantly predicted clinical measure of chorea, and ordinal regression model showed that root mean square and total sway standing feet together significantly predicted clinical measure of tandem walking. InterpretationsRoot mean square measures obtained in sitting and standing using wearable sensors have the potential to serve as biomarkers of postural control impairments in Huntington's disease.

Balanced Nutritional Knowledge Profile and Stress Factors for Karawang Health Science Students during Pandemic

In 2020, mankind is faced with a pandemic problem. If preventive efforts have been made and the covid-19 virus still infected humans, the last step that can be done against the virus is human immunity. So, efforts to increase immunity need to be done, especially during the pandemic. Immunity is related to optimum nutrition. However, optimal nutrition will not be achieved if the related factors are not achieved as well. Therefore, researchers were interested in conducting research to see the knowledge profile of balanced nutritions and stress level as one of the factors in achieving best nutrition during the pandemic. The aim of this study was to determine the knowledge profile of balanced nutrition and stress levels with a sample of health science students at Unsika (Singaperbangsa Karawang University) during the pandemic. This analysis design was a combination of quantitative and qualitative exploration. It was determined purposively and conducted in November-December 2020. From the results of the inquiry, it is known that the profile picture of balanced nutrition knowledge during the pandemic of Unsika Students; 35% of respondents had less intelligence, 61% of the survey participants had moderate insight, and only 4% had a good one. Based on this experiment, it is also known that students got a lot of information from the internet (websites/ articles/ popular social media). An overview of the stress level of Unsika Students; were 35% of respondents at normal stress level, 14% of the samples had mid stress stage, 27% considered as moderate stress phase, 20% included in severe stress class, and 4% got very heavy stress one. The causes of the stress during the pandemic included a slumping economic situation, due to task deadlines and lack of understanding of learning materials, loneliness, boredom, fear of infection of the virus and other personal reasons.