Mid Stages Research Articles

Planting time and variety effects on biomass, harvest index, and yield of irrigated soybean in mid‐Southern United States

AbstractSoybean [Glycine max (L.) Merr.] biomass and grain yield has increased over the past several decades in the mid‐southern United States. This is attributable to technological advances and improved management strategies. However, a better understanding of biomass accumulation and partitioning is needed to improve our knowledge base of varietal growth habits relative to yield, planting date, and harvest index (HI). Field experiments within a split plot arrangement in a randomized complete block design were established in 2017 and 2018 in Stoneville, MS. The study aimed to evaluate the effect of early (late‐April or mid‐May) and late (late‐May) planting on biomass, HI, and yield amongst eight soybean varieties. Soybean total biomass accumulation was collected at multiple development stages, including V4, R2, mid R5, mid R6, and R8, and partitioned into senesced leaves, pods, and seeds. Overall, the planting date had no effect on yield, HI, and biomass accumulation at any of the growth stages. Yet, the interaction between planting date and variety significantly affected biomass accumulation at the mid R5 stage. Contrarily, the variety selection significantly affected yield, HI, and biomass accumulation at all growth stages except mid R6. The total biomass accumulation at R8 was greatest for Asgrow 46X6, Asgrow 4632, Terral 4857X, Terral 48A76, and Credenz 4748, when pooled over planting dates. Averaged across two planting dates, the greatest yield was produced by Terral 48A76, Asgrow 4632, and Asgrow 46X6. Furthermore, averaged across site‐years, HI was greatest for Asgrow 4632 and Terral 48A76. Based on the results of this study, evaluating soybean HI rather than overall biomass accumulation may be more beneficial for variety selection decisions.

The effect of bovine diets and stages of lactation on vitamin K content in butter and Cheddar cheese

This study investigated the vitamin K content in butter and Cheddar cheese produced from the milk of cows fed three different diets: pasture-fed (GRS), total mixed ration (TMR), and partial mixed ration (PMR), across early, mid, and late stages of lactation. Vitamin K1 and menaquinones (MK)-4, 7, and 9 were quantified using high-performance liquid chromatography (HPLC) with a fluorescence detector. Results showed that butter and Cheddar cheese made from higher ratio of pasture-fed milk exhibited significantly higher K1 levels (P<0.05). GRS butter demonstrated the highest MK-4 content (P<0.05), whereas TMR Cheddar cheese showed the highest MK-4 levels (P<0.05), followed by GRS and PMR cheeses. Mid-lactation butter contained higher concentrations of K1 and MK-4 compared to early- or late-lactation stages (P<0.05). Late-lactation Cheddar cheese contained significantly higher K1, MK-4, and MK-9 than early or mid-lactation cheeses (P<0.05). These findings suggest that bovine diets with a higher pasture-fed ratio can enhance the vitamin K content in dairy products.

Seasonal Changes in Salicylic and Jasmonic Acid Levels in Poplar with Differing Stress Responses

Poplars are essential tree species with critical roles in wood production, ecological conservation, environmental protection, and climate change mitigation. Plants of different poplar populations exhibit varying responses to biotic and abiotic stresses, largely driven by phytohormones—key regulators of plant growth and stress responses. Phytohormones control fundamental processes such as cell division, growth, tissue differentiation, organ development, and resistance to environmental challenges such as temperature extremes and drought. In this study, we conducted a quantitative analysis of phytohormone levels in the leaves of two populations of poplar species from the same geographical region over a one-year period, covering the early, mid, and late growth stages. Our results revealed that salicylic acid (SA) and jasmonic acid (JA) concentrations in the stress-resistant poplar (RP) increased significantly during the late growth stage (September), particularly in response to elevated pathogen pressure and fluctuating climatic conditions. In contrast, the susceptible poplar (SP) consistently exhibited lower levels of SA and JA across all growth stages, particularly during the mid-growth stage (characterized by high temperatures and low precipitation) and the late growth stage, when pathogen stress typically intensifies. The limited increase in phytohormone levels in the SP suggests a weaker defense response compared with the RP. These findings demonstrate that climatic factors significantly influence the phytohormonal dynamics in poplars, with the RP exhibiting more robust regulation of SA and JA to mitigate both biotic and abiotic stresses. This study provides a theoretical framework for better understanding the resistance mechanisms in Populus and offers insights for improving stress tolerance in future breeding programs.

Maize (Zea Mays L.) Response to Deficit Irrigation Levels at Different Growth Stages on its Yield and Water Use Efficiency at Koka, Central Rift Valley of Ethiopia

Water scarcity is among the major limitations for crop production. Improving water use efficiency of irrigated crops through water management options is crucial in water scarce areas. Field experiment was carried out at Wondo Genet Agricultural Research Center Koka Research site, to investigate the effect of water stress at different growth stage on yield and water use efficiency of maize. one optimum irrigation and eight growth stage based deficit levels(100% ETC at all growth stages, 75% ETC at all growth stages, 50% ETC at all growth stages, 75% ETC at development growth stage, 50% ETC at development growth stage, 75% ETC at mid growth stage, 50% ETC at mid growth stage, 75% ETC at late growth stage and 50% ETC at late growth stage) were imposed on maize (Zea mays L.) variety Melkassa II as a treatment and laid out in randomized complete block design (RCBD) with three replications. Results indicated that the different levels of growth stage based deficit levels had significant (p&lt;0.01) effect on crop yield, harvest index and water use efficiency. Grain yield reduced with increased stress, whereas water use efficiency was increased with stress level increased. The highest grain yield of 6.4 t/ha and WUE of 1.01 kg/m3 were obtained at 100% ETC and 50% ETC at all growth stages, respectively. Also, 75% ETC at development stage and late stage treatments showed no significant variation with 100% ETC in grain yield. Water use efficiency observed at 75% ETC all growth stages treatment was statistically similar with that of 50% ETC at all growth stages treatment. Grain yield obtained from 50% ETC at mid growth stage was similar with 50% ETC at all growth stages, and water use efficiency was the least and this shows that maize was sensitive to moisture stress at mid growth stage than development and late growth stages. Therefore, maize could be irrigated at 75% ETC at all growth stages and by stressing development or late growth stages up to 50% ETC to increase water use efficiency with a small grain yield reduction for stressed scenario and for non stressed scenario with 100% ETC at all growth stages.

An integrated fast-slow plant and nematode economics spectrum predicts soil organic carbon dynamics during natural restoration.

Aboveground and belowground attributes of terrestrial ecosystems interact to shape carbon (C) cycling. However, plants and soil organisms are usually studied separately, leading to a knowledge gap regarding their coordinated contributions to ecosystem C cycling. We explored whether integrated consideration of plant and nematode traits better explained soil organic C (SOC) dynamics than plant or nematode traits considered separately. Our study system was a space-for-time natural restoration chronosequence following agricultural abandonment in a subtropical region, with pioneer, early, mid and climax stages. We identified an integrated fast-slow trait spectrum encompassing plants and nematodes, demonstrating coordinated shifts from fast strategies in the pioneer stage to slow strategies in the climax stage, corresponding to enhanced SOC dynamics. Joint consideration of plant and nematode traits explained more variation in SOC than by either group alone. Structural equation modeling revealed that the integrated fast-slow trait spectrum influenced SOC through its regulation of microbial traits, including microbial C use efficiency and microbial biomass. Our findings confirm the pivotal role of plant-nematode trait coordination in modulating ecosystem C cycling and highlight the value of incorporating belowground traits into biogeochemical cycling under global change scenarios.

Development of growth-stage specific crop coefficient for drip irrigated wheat crop grown in climatic conditions of Jalandhar, Punjab

The crop coefficient (Kc) values given in FAO-56 report need to corrected for local conditions due to differences in climate, soil types, and water management practices. Therefore, present study was undertaken to develop growth-stage-specific Kc values of wheat grown under drip irrigated conditions for two years (2022-23 and 2023-24) at Jalandhar, Punjab. The developed Kc values are 0.36, 0.77, 1.05, 0.69, and 0.25 during initial, development, mid, late, and end growth stages, respectively. These average Kc values can be effectively utilized to schedule irrigation for drip-irrigated wheat crops in the Jalandhar region of Punjab. Irrigating wheat crop under drip irrigation with developed Kc values not only enhances grain yield by 36% but also improves IWUE by three times and saves 61% use of irrigation water as compared to conventional irrigation practices (flood irrigation).

The role of nutritional factors in transitioning between early, mid, and late stages of age-related macular degeneration: prospective longitudinal analysis

BackgroundTransitions between different stages of age-related macular degeneration (AMD) are not completely captured by traditional survival models with an end point of advanced AMD. ObjectivesThis study aimed to explore the transitions from early and intermediate AMD to higher nonadvanced and advanced stages and determine the contributions of nutritional factors to these outcomes. MethodsEyes with early or intermediate AMD at baseline, classified according to the Age-Related Eye Disease Study severity score, were included in this prospective longitudinal analysis. Foods and the biologically active nutrients associated with AMD [green leafy vegetables, fish, lutein/zeaxanthin (LZ), and ω-3 (n–3) fatty acids] were determined by a baseline food frequency questionnaire. Progression was defined as eyes transitioning to higher severity groups including nonadvanced and advanced stages over 5 y, confirmed at 2 consecutive visits. Cox proportional hazards models for foods and nutrients were analyzed adjusting for demographics, lifestyle, baseline macular status, a family history of AMD, caloric intake, and genetic risk. ResultsAmong 2697 eyes, 616 (23%) progressed to higher severity groups. In the food group model, higher intake of green leafy vegetables reduced incidence of transitions {hazard ratio [HR] (≥2.7 servings/wk compared with none): 0.75; 95% confidence interval [CI]: 0.59, 0.96; P = 0.02}. Higher fish intake was also protective [HR (greater than two 4-ounce servings/wk compared with <2): 0.79; 95% CI: 0.65, 0.95; P = 0.01]. In the nutrient model, LZ intake was protective [HR (≥2 mg/d compared with <2): 0.76; 95% CI: 0.60, 0.96; P = 0.02]. Higher intake of ω-3 fatty acids also tended to be beneficial [HR (≥0.7 g/wk compared with <0.7): 0.85; 95% CI: 0.71, 1.01; P = 0.06]. ConclusionsIncreased consumption of green leafy vegetables, LZ, and fish nutritionally rich in ω-3 fatty acids during the initial stages of AMD may reduce rates of progression to higher severity of this debilitating disease.This trial was registered at clinicaltrials.gov as NCT00594672.

Incubation time, embryonic development and the vomeronasal organ of the Laysan albatross (Phoebastria immutabilis).

The effect of lengthened incubation periods on embryonic development, especially vestigial structures, is poorly understood. An example of which is the avesuchian vomeronasal organ (VNO), a nasal chemosensory organ found in many tetrapods but absent in adult avesuchians (crocodilians and birds) in whom it is presumed to be a transitory fetal structure. The Laysan Albatross (Phoebastria immutabilis) has an incubation period of their eggs of about 65 days. This incubation period is twice that of domestic fowl, wherein a putative VNO has been documented as an epithelial thickening. The purpose of this study is to document the development of a putative VNO in the albatross. Serial histological sections of nine albatross embryonic heads, across 6 stages (representing days 19 to 32: stages 31-39), were examined. A paired putative VNO was present as a short, tubular structure in the anterodorsal aspect on either side of the nasal septum from stage 32 onwards, getting steadily longer in later specimens. At the earliest stages, the epithelial walls of the tube resemble a neuroepithelium, but then becomes thinner and simpler in morphology. Based on our available age range, it is unclear whether it persists as a rudimentary structure (like that of the human) or if it is a transitory structure (like in chickens) in these mid embryonic stages. Though future studies must determine the fate of the Laysan albatross VNO (e.g., is it retained postnatally?), the role of incubation period length on embryonic development is a bigger question to be explored.

Interwell interference model of horizontal wells in shale gas reservoirs based on multi-connected boundary element method

Due to the wide application of closely spaced multi-well horizontal pads for developing unconventional gas reservoirs, interference between wells becomes a significant concern. Communication between wells mainly occurs through natural fractures. However, previous studies have found that interwell communication through natural fractures is varied, and non-communication also appears in the mid and late stages of production due to natural fracture closure. This study proposes a boundary element method for coupling multi-connected regions for the first time. Using this method, we coupled multiple flow fields to establish dual-well models with various connectivity conditions of the stimulated reservoir volume (SRV) region. These models also take into consideration of adsorption and desorption mechanism of natural gas as well as the impact of fracturing fluid retention.The study found that when considering the non-communication of SRV regions between multi-well horizontal pads, the transient behavior of the targeted well exhibits a transitional flow stage occurring before the well interference flow stage. In addition, sensitivity analysis shows that the well spacing and production regime, as well as the connectivity conditions of the SRV region, affect the timing of interwell interference. Meanwhile, the productivity of the two wells, reservoir properties, and fracturing operations affect the intensity of interwell interference.

Behind the Curtain of Abnormal Placentation in Pre-Eclampsia: From Molecular Mechanisms to Histological Hallmarks.

Successful human pregnancy needs several highly controlled steps to guarantee an oocyte's fertilization, the embryo's pre-implantation development, and its subsequent implantation into the uterine wall. The subsequent placenta development ensures adequate fetal nutrition and oxygenation, with the trophoblast being the first cell lineage to differentiate during this process. The placenta sustains the growth of the fetus by providing it with oxygen and nutrients and removing waste products. It is not surprising that issues with the early development of the placenta can lead to common pregnancy disorders, such as recurrent miscarriage, fetal growth restriction, pre-eclampsia, and stillbirth. Understanding the normal development of the human placenta is essential for recognizing and contextualizing any pathological aberrations that may occur. The effects of these issues may not become apparent until later in pregnancy, during the mid or advanced stages. This review discusses the process of the embryo implantation phase, the molecular mechanisms involved, and the abnormalities in those mechanisms that are thought to contribute to the development of pre-eclampsia. The review also covers the histological hallmarks of pre-eclampsia as found during the examination of placental tissue from pre-eclampsia patients.

Influence of Opacity Depth on Big Bubble Formation During Deep Anterior Lamellar Keratoplasty in Corneal Stromal Scars.

To identify the key preoperative predictors of big bubble (BB) formation during deep anterior lamellar keratoplasty in patients with corneal stromal scars (CSS). This retrospective cohort study included consecutive patients with CSS after infective keratitis who underwent BB-deep anterior lamellar keratoplasty between January 2021 and July 2023 at a tertiary referral center. Topographic and tomographic data were collected to compare the rates and types of BB formations. Anterior segment optical coherence tomography (AS-OCT) was employed to assess the maximum depth of opacity by dividing the stroma into 3 zones of equal thickness: anterior (stage A), mid (stage B), and posterior stroma (stage C). Multivariate logistic regression analysis was performed to identify the potential preoperative predictors of bubble formation. Pneumatic dissection was achieved in 13 of 33 eyes (39.4%), with 11 BB type 1 eyes (33.3%) and 2 BB type 2 eyes (6.1%). According to AS-OCT grading, bubble formation was more frequent with CSS involving more superficial stromal layers (P <0.032). In the eyes with stage C, bubble formation failed 12 out of 14 times (85.7%, P <0.026). Spearman correlation showed that bubble formation was inversely associated with the AS-OCT grading (rho = -0.443, P = 0.001). After logistic regression analysis, AS-OCT grading was found to be the sole factor that predicted bubble formation (coeff. -1.58, confidence interval 95% -3.03 to -0.12, P = 0.034). Depth of opacity in CSS was the key determinant for predicting the success of pneumatic dissection, as advanced AS-OCT stages are strongly associated with BB failure.

Taxonomic and functional diversity along successional stages on post-coalmine spoil heaps

Coal is the most abundant fossil fuel in Europe, but the excavation of hard coal has covered large areas with disposed rock waste, and turned the natural habitats into disturbed novel ecosystems with harsh conditions differ in time and space. To examine the spontaneous complex successional gradient, we studied a large number of post coalmine heaps in Upper Silesia, which differ in vegetation type and age. Cluster analysis based on plant community composition (367 species in total) separated all surveyed plots on coal mining spoil heaps with herbaceous vegetation from Late Stage (LS) forests aged 14–56 years. Furthermore, the herbaceous vegetation was sub-grouped to three stages: Initial Stage (IS) aged 2–5 years, Early Stage (ES) aged 3–8 years and Mid-Stage (MS) aged 5–12 years. MS vegetation was characterised by the highest species richness and diversity (47 and 2.79) compared to ES (30 and 2.18) and IS (9 and 1.6), but higher species number and a similar diversity index occurred in LS (37 and 2.81). Functional diversity (FD) and community weighted mean (CWM) of nine functional traits showed higher (23.1) functional richness, higher (0.72) functional divergence, higher (4.5) functional dispersion, and higher value (24.4) of Rao’s quadratic entropy in LS compared to those calculated from the first three stages. Species at the initial successional stage (IS) were characterised by lower canopy height, seed mass, higher lateral spread, and specific leaf area (SLA). Additionally, the lowest (0.22 mg CO2 per hour per square metre) soil respiration (Sr) rate was recorded from IS compared to (0.53, 0.82 and 1.00) from ES, LS and MS, respectively. The soil water content (SWC) was the most important factor affecting the soil respiration, while the soil temperature (St) did not follow the well-studied relationship between soil respiration and soil temperature. Our spatial and temporal analyses illustrated changes in plant community assembly processes in the course of spontaneous vegetation succession on post coalmine spoil heaps. The importance of trait mediated abiotic filtration in community assembly in initial-, early-, and mid-stages of succession with an increase in competitive exclusion at the late successional stage was emphasized.

Kaempferia parviflora extract and its component polymethoxyflavones suppress adipogenic differentiation of human bone marrow-derived mesenchymal stem cells via the AMPK pathway.

Kaempferia parviflora Wall. ex. Baker (KP) has been reported to exhibit anti-obesity effects. However, the detailed mechanism of the anti-obesity effect of KP extract (KPE) is yet to be clarified. Here, we investigated the effect of KPE and its component polymethoxyflavones (PMFs) on the adipogenic differentiation of human mesenchymal stem cells (MSCs). KPE and PMFs fraction (2.5µg/mL) significantly inhibited lipid and triacylglyceride accumulation in MSCs; lipid accumulation in MSCs was suppressed during the early stages of differentiation (days 0-3) but not during the mid (days 3-7) or late (days 7-14) stages. Treatment with KPE and PMFs fractions significantly suppressed peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and various adipogenic metabolic factors. Treatment with KPE and PMFs fraction induced the activation of AMP-activated protein kinase (AMPK) signaling, and pretreatment with an AMPK signaling inhibitor significantly attenuated KPE- and PMFs fraction-induced suppression of lipid formation. Our findings demonstrate that KPE and PMFs fraction inhibit lipid formation by inhibiting the differentiation of undifferentiated MSCs into adipocyte lineages via AMPK signaling, and this may be the mechanism underlying the anti-obesity effects of KPE and PMFs. Our study lays the foundation for the elucidation of the anti-obesity mechanism of KPE and PMFs.

Carbon fluxes and water-use efficiency in a Pinus tabuliformis plantation in Northeast China and their relationship to drought

The impact of extreme weather events on carbon fluxes and water-use efficiency (WUE) in revegetated areas under water-limited conditions is poorly understood. We analyzed changes in carbon fluxes and WUE over three years of eddy-covariance measurements in a Pinus tabuliformis plantation in Northeast China to investigate carbon fluxes and WUE responses to drought events at different time scales. Mean annual net ecosystem exchange (NEE), gross primary production (GPP), and ecosystem respiration (Re) were −368.48, 1042.42, and 673.94 g C m−2, respectively. Drought events increased NEE, as GPP was more sensitive to water stress than Re at different growing stages. Mean annual WUE was 2.46 g C kg−1 H2O, and plant phenology played a key role in WUE responses to drought. Water stress had negative and positive effects on daily WUE at the early and late growing stages, respectively, and daily WUE was generally insensitive to drought at the mid growing stage. A lagged effect existed in the carbon fluxes and WUE dynamics after drought events at various time scales. Water stress at the early growing stage was more important than that at other growing stages on annual carbon sequestration and WUE, as it dominated canopy growth in the current year. The annual mean normalized difference vegetation index controlled interannual variations in carbon fluxes and WUE in the plantation. Our findings contribute to the prediction of possible changes in carbon and water fluxes under climate warming in the afforested areas of Northeast China.

Expression and localization of anti-Müllerian hormone and its receptors in bovine corpus luteum

Although anti-Müllerian hormone (AMH) is involved in the regulation of granulosa cell function in female animals, its role in tissues other than ovarian follicles remains poorly understood. It has also been suggested that cows with high circulating AMH concentrations have increased fertility; however, the mechanism has not been elucidated. This study was conducted to identify the presence of the AMH-signaling system and its target cells in the bovine corpus luteum formed from an ovulated follicle. Immunoblotting revealed that the proteolytically cleaved C-terminal region in AMH (AMHC), a biologically active peptide, was present in trace amounts in the early corpus luteum and significantly increased during the mid to regressed stages. AMHC and cleaved N-terminal region (AMHN) in AMH generate a noncovalent isoform that improves the activity of AMH signaling. An immunohistochemical analysis revealed that AMHC, AMHN, and type II AMH receptor (AMHR2) were localized to luteal cells during the entire estrous cycle. AMH in the corpus luteum seemed to be newly synthesized since AMH expression was detected. These findings suggest that AMH signaling is involved in the regulation of luteal cell function through an autocrine and post-translational processing mechanism. The level of AMHR2 and mRNA expression of AMHR2 and type I AMH receptors (activin-like kinase 2, 3, and 6) were highest in the mid stage. Thus, AMH signaling in the corpus luteum may also be regulated by changes in the receptor levels. Since the transforming growth factor-beta superfamily, to which AMH belongs, is a multifunctional polypeptide growth factor, further studies are needed to evaluate whether AMH signaling has a role in facilitating or inhibiting luteal cell functions.

Research Progress in Traditional Chinese Medicine Treatment of Knee Osteoarthritis

Knee osteoarthritis (KOA) is a degenerative disease characterized by pathological changes in the cartilage and subchondral bone of the knee joint. Initially, knee joint pain is the main symptom, and in the later stages, deformities and mobility disorders may occur. Modern medicine lacks effective treatment methods for early and mid stage KOA, and has drawbacks such as significant side effects and high costs. A large number of studies have confirmed that traditional Chinese medicine has unique advantages in treating KOA, and has obvious advantages such as small trauma, low cost, and definite therapeutic effect, which are gradually being accepted by a large number of KOA patients. The commonly used methods for preventing and treating KOA with traditional Chinese medicine in clinical practice include: oral administration of traditional Chinese medicine, external application of traditional Chinese medicine, fumigation, acupuncture therapy, massage therapy for regulating tendons, and targeted penetration therapy of traditional Chinese medicine. According to relevant literature, traditional Chinese medicine can significantly alleviate knee joint symptoms and improve knee joint function in patients with knee osteoarthritis, which is of great significance for the prevention and treatment of knee osteoarthritis. This article provides a review of the research progress in the prevention and treatment of KOA using traditional Chinese medicine in recent years.

Effect of parity and lactation stage on milk yield, udder and teat morphometric traits of Friesian-Bunaji crossed cows

Abstract. Data for this study were collected from 40 multiparous (F1) Friesian x Bunaji cows at the dairy herd of the National Animal Production Research Institute (NAPRI) Shika, Nigeria, to assess the effect of parity and lactation stage on milk yield, udder and teat morphometric traits of Friesian-Bunaji crossed cows as well the relationships between the parameters measured. The data collected include udder circumference (UC), udder depth (UD), udder width (UW), fore teat length (FTL), and hind teat length (HTL) and were subjected to Analysis of Variance (ANOVA). The means with significant difference were separated using Duncan Multiple Range Test modelled in SAS (2004) statistical package and the correlation analysis was performed using correlation procedure in SAS to identify relationships between morphometric traits and milk yield. The results obtained showed that parity had significant (p&lt;0.05) effect on udder circumference, hind teat length and milk yield. The highest udder circumference (23.60±2.50 cm) and hind teat length (2.80±0.34 cm), respectively, were found in parity 5 and the least ones were obtained in parity 1. Parity 2 had significantly (p&lt;0.05) higher milk yield (8.60±0.72 litres) followed by parity 4 (7.13±1.14 litres) but similar to parity 3 (6.67±0.58 litres) and the least (6.20±1.24 litre) was obtained in parity 5 (6.20±1.24 litres) but similar with parity 1 (6.25±0.75 litres). The results for lactation stage revealed no significant (p&gt;0.05) difference in all the parameters except milk yield. The early lactation stage had significantly (p&lt;0.05) higher milk yield (8.15±0.35 litre) followed by mid lactation stage (5.80±0.58 litre) but similar with the milk yield (4.38±0.86 litre) at late lactation stage. The results obtained for correlation indicated that the udder and teat morphometric traits and the milk yield measured showed varying degrees of relationships. The phenotypic correlations were positive and negative, low to high ranging from 0.00 to 0.69. The highest correlation coefficient (r=0.69; p&lt;0.01) was obtained between udder circumference and udder depth. All the udder and teat morphometric traits measured were negatively correlated with the milk yield except udder width (UW) and had no significant (p&gt;0.05) difference. Based on the results in this study, it can be concluded that parity has effect on udder circumference, hind teat length and milk yield with second parity having the highest milk yield 8.60±0.72 litre/day), while lactation stage had no effect in all the morphometric traits except on milk yield with the early stage having the highest milk yield (8.15±0.35 litre/day). The second parity and early lactation stage are recommended for high milk yield in Friesian x Bunaji cows in the studied area.

Integrative Metabolomic and Transcriptomic Analysis Elucidates That the Mechanism of Phytohormones Regulates Floral Bud Development in Alfalfa.

Floral bud growth influences seed yield and quality; however, the molecular mechanism underlying the development of floral buds in alfalfa (Medicago sativa) is still unclear. Here, we comprehensively analyzed the transcriptome and targeted metabolome across the early, mid, and late bud developmental stages (D1, D2, and D3) in alfalfa. The metabolomic results revealed that gibberellin (GA), auxin (IAA), cytokinin (CK), and jasmonic acid (JA) might play an essential role in the developmental stages of floral bud in alfalfa. Moreover, we identified some key genes associated with GA, IAA, CK, and JA biosynthesis, including CPS, KS, GA20ox, GA3ox, GA2ox, YUCCA6, amid, ALDH, IPT, CYP735A, LOX, AOC, OPR, MFP2, and JMT. Additionally, many candidate genes were detected in the GA, IAA, CK, and JA signaling pathways, including GID1, DELLA, TF, AUX1, AUX/IAA, ARF, GH3, SAUR, AHP, B-ARR, A-ARR, JAR1, JAZ, and MYC2. Furthermore, some TFs related to flower growth were screened in three groups, such as AP2/ERF-ERF, MYB, MADS-M-type, bHLH, NAC, WRKY, HSF, and LFY. The findings of this study revealed the potential mechanism of floral bud differentiation and development in alfalfa and established a theoretical foundation for improving the seed yield of alfalfa.

Is redox zonation an appropriate method for determining the stage of natural remediation in deep contaminated groundwater?

Groundwater contamination resulting from petroleum development poses a significant threat to drinking water sources, especially in developing countries. In situ natural remediation methods, including microbiological processes, have gained popularity for the reduction of groundwater contaminants. However, assessing the stage of remediation in deep contaminated groundwater is challenging and costly due to the complexity of diverse geological conditions and unknown initial concentrations of contaminants. This research proposes that redox zonation may be a more convenient and comprehensive indicator than the concentration of contaminants for determining the stage of natural remediation in deep groundwater. The combination of sequencing microbial composition using the high-throughput 16S rRNA gene and function predicted by FAPROTAX is a useful approach to determining the redox conditions of different contaminated groundwater. The sulfate-reducing environment, represented by Desulfobacteraceae, Peptococcaceae, Desulfovibrionaceae, and Desulfohalobiaceae could be used as characteristic early stages of remediation for produced water contamination in wells with high concentrations of SO42−, benzene, and salinity. The nitrate-reducing environment, enriched with microorganisms related to denitrification, sulfur-oxidizing, and methanophilic microorganisms could be indicative of the mid stages of in situ bioremediation. The oxygen reduction environment, enriched with oligotrophic and pathogenic Sphingomonadaceae, Caulobacteraceae, Syntrophaceae, Legionellales, Moraxellaceae, and Coxiellaceae, could be indicative of the late stages of remediation. This comprehensive approach could provide valuable insights into the process of natural remediation and facilitate improved environmental management in areas of deep contaminated groundwater.

Insights into Disease Progression of Translational Preclinical Rat Model of Interstitial Pulmonary Fibrosis through Endpoint Analysis.

Idiopathic pulmonary fibrosis (IPF) is a devastating interstitial lung disease characterized by the relentless deposition of extracellular matrix (ECM), causing lung distortions and dysfunction. Animal models of human IPF can provide great insight into the mechanistic pathways underlying disease progression and a means for evaluating novel therapeutic approaches. In this study, we describe the effect of bleomycin concentration on disease progression in the classical rat bleomycin model. In a dose-response study (1.5, 2, 2.5 U/kg i.t), we characterized lung fibrosis at day 14 after bleomycin challenge using endpoints including clinical signs, inflammatory cell infiltration, collagen content, and bronchoalveolar lavage fluid-soluble profibrotic mediators. Furthermore, we investigated fibrotic disease progression after 2 U/kg i.t. bleomycin administration at days 3, 7, and 14 by quantifying the expression of clinically relevant signaling molecules and pathways, epithelial mesenchymal transition (EMT) biomarkers, ECM components, and histopathology of the lung. A single bleomycin challenge resulted in a progressive fibrotic response in rat lung tissue over 14 days based on lung collagen content, histopathological changes, and modified Ashcroft score. The early fibrogenesis phase (days 3 to 7) is associated with an increase in profibrotic mediators including TGFβ1, IL6, TNFα, IL1β, CINC1, WISP1, VEGF, and TIMP1. In the mid and late fibrotic stages, the TGFβ/Smad and PDGF/AKT signaling pathways are involved, and clinically relevant proteins targeting galectin-3, LPA1, transglutaminase-2, and lysyl oxidase 2 are upregulated on days 7 and 14. Between days 7 and 14, the expressions of vimentin and α-SMA proteins increase, which is a sign of EMT activation. We confirmed ECM formation by increased expressions of procollagen-1Aα, procollagen-3Aα, fibronectin, and CTGF in the lung on days 7 and 14. Our data provide insights on a complex network of several soluble mediators, clinically relevant signaling pathways, and target proteins that contribute to drive the progressive fibrotic phenotype from the early to late phase (active) in the rat bleomycin model. The framework of endpoints of our study highlights the translational value for pharmacological interventions and mechanistic studies using this model.