Seasonal Changes in Salicylic and Jasmonic Acid Levels in Poplar with Differing Stress Responses

Abstract

Poplars are essential tree species with critical roles in wood production, ecological conservation, environmental protection, and climate change mitigation. Plants of different poplar populations exhibit varying responses to biotic and abiotic stresses, largely driven by phytohormones—key regulators of plant growth and stress responses. Phytohormones control fundamental processes such as cell division, growth, tissue differentiation, organ development, and resistance to environmental challenges such as temperature extremes and drought. In this study, we conducted a quantitative analysis of phytohormone levels in the leaves of two populations of poplar species from the same geographical region over a one-year period, covering the early, mid, and late growth stages. Our results revealed that salicylic acid (SA) and jasmonic acid (JA) concentrations in the stress-resistant poplar (RP) increased significantly during the late growth stage (September), particularly in response to elevated pathogen pressure and fluctuating climatic conditions. In contrast, the susceptible poplar (SP) consistently exhibited lower levels of SA and JA across all growth stages, particularly during the mid-growth stage (characterized by high temperatures and low precipitation) and the late growth stage, when pathogen stress typically intensifies. The limited increase in phytohormone levels in the SP suggests a weaker defense response compared with the RP. These findings demonstrate that climatic factors significantly influence the phytohormonal dynamics in poplars, with the RP exhibiting more robust regulation of SA and JA to mitigate both biotic and abiotic stresses. This study provides a theoretical framework for better understanding the resistance mechanisms in Populus and offers insights for improving stress tolerance in future breeding programs.