The success of 3D printing relies on developing components with desired strength that heat treatment processes can further improve. While SS 316L is a widely used structural material for several industrial applications, the fatigue behaviour of its 3D-printed version is investigated herein. The low cycle fatigue (LCF) behaviour ofheat-treated (HT)3D-printed SS 316L specimens was carried out and compared with thewithout heat-treated (WHT)specimens thereof. The heat treatment considerably affected its LCF behaviour, which can be attributed to the microstructural changes post heat treatment. The cyclic softening is observed in both HT and WHT specimens. However, the degree of softening is lower (12.35 %) for HT compared to WHT specimens (25.30 %) and 62 % higher fatigue life for HT compared to WHT specimens. Further, the hardness values obtained are 176 and 169 HV for WHT and HT, respectively, while it is 238 and 223 HV for the same, before and after fatigue tests. Fractography revealed fewer pores and reduced fatigue striations in the HT specimens. Considering the Chaboche non-linear model, finite element modelling was employed to capture the specimens’ fatigue behaviour. The proposed model is found to be suitable for predicting the cyclic behaviour of 3D-printed austenitic steels.