Nectar production in wood betony (Stachys officinalis L), grown under differ-microclimatic habitat conditions (forest and experimental field), was analyzed. Study of tar production included determining of the total daily nectar amount (in 24 hours) per flower, the diurnal dynamics of nectar secretion (nectar amount secreted per flower at two hour intervals during the day), and nectar sugar concentration. The aim of this study was to evaluate the influence of microclimatic parameters humidity, air temperature and evaporation) on the secretion process and nectar sugar concentration. Comparative analyses of nectar secretion in Stachys officinalis, grown in two environmentally different habitats, confirmed that this process varied as a function of micro-climatic habitat conditions, and did not reveal a close relationship between these conditions and sugar concentration in nectar. A diurnal model of nectar secretion with two secretion peaks was found. A higher amount of nectar per flower, with an increasing tendency during the first half of the day, and a decreasing tendency during the afternoon was measured in forest conditions (lower temperature, higher relative air humidity and lower evaporation).
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