Method For Rejuvenation Research Articles

Practical research on developing methods for danchi rejuvenation utilizing “danchi power”

Practical research on developing methods for danchi rejuvenation utilizing “danchi power”

Evaluation of Methods of Pasture Rejuvenation for Improved Forage Production

Evaluation of Methods of Pasture Rejuvenation for Improved Forage Production

Using dermal fillers for vaginal skin rejuvenation

The number of women requesting genital enhancement treatments is steadily increasing. One popular and effective method of vaginal rejuvenation is the use of dermal fillers to plump the labia majora. This procedure, sometimes called the ‘labial puff’, uses hyaluronic acid products to plump the area, reducing the signs of ageing skin. Women who express an interest in this treatment should be given a full understanding of the procedure, how it works and the realistic outcomes. This article aims to bring attention to this treatment option and highlights areas for improvement to deliver a more patient-friendly approach, as well as to assist the practitioner in improving their technique. The advantages and disadvantages of injecting dermal fillers into this delicate area are explored. This treatment has potential to improve women's confidence and sexual wellbeing, and should therefore be considered as a possible treatment option for women presenting with concerns in this area.

Adipose Stromal Vascular Fraction Gel Grafting: A New Method for Tissue Volumization and Rejuvenation.

The clinical outcomes of fat grafting vary and are technique-dependent. Stromal vascular fraction (SVF) gel is a novel, mechanically processed fat product with high concentrations of adipose tissue-derived stem cells and other SVF cells. This study evaluated the volumization and rejuvenation effects of SVF-gel. This study evaluated the volumization and rejuvenation effects of SVF-gel. This retrospective, single-center study included 126 patients who underwent SVF-gel grafting and 78 who underwent conventional lipoinjection for various indications from March 2015 to February 2017. Patient satisfaction and secondary surgery rates were evaluated. Samples of transferred SVF-gel were harvested and examined histologically. All patients showed improvements in facial augmentation and contour. Patients in the SVF-gel group experienced mild postoperative swelling and a low secondary surgery rate (10.9%). Assessment of patient-rated satisfaction on a 5-point Likert scale found that 77.3% of patients in the SVF-gel group were satisfied (54.5%) or very satisfied (22.8%) with their outcomes. By comparison, 53.8% of patients who underwent conventional lipoinjection were satisfied (48.7%) or very satisfied (5.1%). Moreover, SVF-gel showed effective antiwrinkle and skin rejuvenation effects. Hematoxylin-eosin staining showed a normal adipose tissue structure in transferred SVF-gel. Stromal vascular fraction gel is effective for both volumization and rejuvenation, and may be superior to conventional lipoinjection for facial recontouring.

Методические аспекты изучения биоресурсных коллекций редких и хозяйственно ценных растений

A full range of classical and modern methods of evaluation of biological peculiarities and economic characteristics of samples including in the bioresource collection is presented. The main directions of their study: seasonal development, ontomorphogenesis, reproductive biology and genetic certi fi cation. It is shown that for trees, shrubs and perennials forming shoots of different types during the vegetative period, it is preferable to construct separate phonological spectra of vegetative and generative development. The adaptive potential is estimated on the basis of studying the structure of shoot systems and preservation of them in the unfavorable periods of weather; the formation of life form in ontogenesis; and upon reaching the generative state, it is done, taking into account the peculiarities of intrabud and extrabud organogeneses. The applied aspect of these studies is substantiation of time and methods of rejuvenation of objects for preservation of them in the bioresource collections. When studying reproductive biology, the potential importance of samples for breeding as paternal and maternal parental forms is determined. The fertility and viability of pollen are studied in detail in paternal forms, the terms and methods of pollen storage are elaborated. Fruit setting with different methods of pollination is evaluated in maternal forms. The fi nal stage is to estimate the potential and actual seed productivity, determine the type of dormancy of seeds and develop ways to overcome it. Based on the example of model objects, representatives of generic complexes of Rhaponticum and Trollius, the advantages of molecular-genetic method of ISSR-marking, based on the analysis of polymorphic DNA sites between microsatellites are shown. Its use allows to detect the greater number of polymorphic loci, and does not require prior cloning and sequencing of fragments for the selection of primers. The chemical composition of three species for cellulose, lignin, pentosans and ash content was studied in the model Miscanthus object, which is a promising bioenergetic culture. It was shown that samples with high cellulose content but low lignin content are of interest for technological processes.

A Prospective Study of the Safety and Efficacy of a Microneedle Fractional Radiofrequency System for Global Facial Photoaging in Chinese Patients.

Facial photoaging has become a major cosmetic concern, and the microneedle fractional radiofrequency system (MFRS) is a novel method for rejuvenation that combines radiofrequency and microneedles. This study prospectively evaluated the efficacy and safety of an MFRS in the treatment of facial photoaged skin in Chinese patients. Twenty-seven patients with moderate facial photoaging were recruited and received 3 treatments at 4-week intervals. Blinded clinical assessment was performed by 2 independent dermatologists on a 5-point global photoaging scale (GPS). Patients were also questioned on the extent of improvement of rhytides, skin tightening, and complexion with a 4-point global aesthetic improvement scale (GAIS) and satisfaction based on a 5-point scale. Adverse events and pain scores were also evaluated. Compared with the baseline, there was a significant improvement in facial photoaged skin after 3 treatments, and these positive outcomes were maintained up to the 6-month follow-up, according to the GPS and GAIS scores. Most patients were satisfied with the treatment and reported mild to moderate pain and adverse effects. This MFRS is effective for facial skin rejuvenation in Chinese patients. The therapy also seems safe and well tolerated.

Forage Production and Economic Performance of Pasture Rejuvenation Methods in Northern Alberta, Canada

Producing high quality forage and maintaining productive pastures is a major challenge that beef producers encounter, as rejuvenation is a complex and costly challenge. This is part of a series of papers looking at potential options and methods of rejuvenation to improve the productivity of older forage stands in northern Alberta. The methods of rejuvenation investigated were sub-soiling, break & re-seeding, a combination of manure application plus subsoiling, high stock density grazing, bale grazing, pasture rest, as well as direct seeding in spring and fall. In this series, forage dry matter (DM) yield, forage nutritive value and economic performance are presented and discussed. The top 5 forage DM yielders were bale grazing, manure + subsoil in fall, break & re-seeding, high stock density grazing and fertilizer application in that order. In both years, bale grazing consistently produced higher forage DM yield than other methods including control, with bale grazing giving up to 100% higher yield at site-1 and 219% at site- 2 for the 2-year total forage DM. Most forage nutritive value parameters measured were similar for the rejuvenation methods investigated. A simplified economic analysis done in this study showed that the direct input cost of rejuvenation an old forage stand was higher with the break & re-seeding method than other methods. However, for bale grazing, when the cost of hay bales used was factored in, then the cost of bale grazing far exceeded those of other methods including break & re-seeding. The implications of the results obtained in this study in relation to beef cattle production system are highlighted.

On-farm Assessments of Pasture Rejuvenation Methods on Soil Quality Indicators in Northern Alberta (Canada)

The study was aimed at providing livestock producers with options on practical methods to improve soil quality of pastures for improved forage production and livestock carrying capacity. The study was carried out on-farm from 2015 to 2017 at two sites in northern Alberta, Canada. The methods of rejuvenation (treatments) evaluated were: sub-soiling, break & re-seed (pasture renewal), a combination of manure application plus subsoiling, pasture rest, inorganic fertilizer application, high stock density grazing and bale grazing. A check/control treatment was included for comparison. For break & re-seed, the forage mixture seeded (16.8 kg ha-1) consisted of 60% grasses and 40% legumes. Overall, bale grazing improved soil organic matter (SOM) by up to 3.80% over other methods including check. In terms of soil compaction penetration resistance, water infiltration rate, water content and nutrients particularly N, P and K, bale grazing system as a method of rejuvenating old pastures significantly showed higher values than check at both sites. Without having to break and re-seed old pastures, the first option that livestock producers would have success with in improving soil quality for better pasture productivity would be bale grazing. The next two methods or rejuvenation strategies with great potential for improving soil conditions for pastures would be a combination of manure application plus subsoil in fall and high stock density grazing.

Aesthetics and Rejuvenation of the Temple.

The temples are an often overlooked, but important element of facial rejuvenation. The anatomy of the temple should be understood prior to any intervention in this location. Multimodal treatment to re-establish youthful convexity, proper hairline position, and correct actinic damage is recommended for optimal results. Important anatomy, aesthetic goals, and methods of rejuvenation are reviewed in detail.

Rejuvenation of soft material-actuator

Abstract

Blepharoplasty: Anatomy, Planning, Techniques, and Safety.

Blepharoplasty is one of the most commonly performed cosmetic surgical procedures. To date it remains the most powerful method of periorbital rejuvenation when compared to other nonsurgical modalities, especially in the aging face. Despite that, the procedure has its shortcomings that include a steep learning curve, prolonged recovery, and potential for appearance and life-changing complications. Attaining successful outcomes relies on a solid understanding of facial topography, patient and technique selection, and, when appropriate, following a conservative approach. Modern blepharoplasty relies on tissue conservation and volume enhancement rather than aggressive removal. This concept was conceived after the realization that older techniques resulted in a hollowed appearance, which accentuated the aging process. It was further reinforced by advances in knowledge of periorbital anatomy and aging changes. This Continuing Medical Education article will detail periorbital surgical anatomy, preoperative planning, and varied blepharoplasty approaches and techniques, with an emphasis on safety and tailoring the procedure to the patient's anatomy.

Mesotherapy – a method of facial skin rejuvenation from an interdisciplinary perspective on improving facial aesthetics

Mesotherapy has been used in medicine since the 1950s and for aesthetic procedures since the 1970s. In the medical literature there are numerous reports about the positive effect of mesotherapy with regard to rejuvenating and improving the appearance of facial skin (reduction of fine wrinkles and discolouration, facial contour correction, improvement in skin tightness and elasticity). The variety of formulations used and the different techniques for administering them mean that the subjectively observed effects of mesotherapy cannot be objectively verified. To date, only a few studies have been published in the international literature where histopathological, ultrasound, and electron microscopy examinations were performed to confirm the efficacy of this method. Most studies, however, have been based on assessing subjective improvement. The aim of this paper was to review literature on the subject, namely 15 full-text reports on the effects of mesotherapy in the treatment of facial skin. The findings of the review show that the studies published to date are based primarily on subjective assessment methods, presenting the results of mesotherapy procedures through an analysis of "before and after" medical photographic documentation. It would seem necessary that further research should be conducted based on a unified protocol.

The Chapter on Rasāyana (Medications for Rejuvenation) in Miʿrāj al-duʿāʾ, a Shiʿite Text from the 12th/18th Century

Miʿrāj al-duʿāʾ wa-mirʾāt al-dawāʾ (“The Ascent of Prayer and the Mirror of Medication”) by Muḥammad ʿAlī al-Qazwīnī, a Shiʿite presumably working in eastern Iraq in the eighteenth century, gathers information on methods for rejuvenation and longevity from different traditions: traditional Islamic (mainly Shiʿite), Greek, and Indian. The last of these are a set of recipes for rasayanas, herbal and chemical recipes drawn from Indian sources. Though some rasayanas are mentioned in earlier Arabic treatises, the collection displayed in Miʿrāj al-duʿāʾ is by far the most extensive. Hardly any are mentioned in earlier Arabic texts. Miʿrāj al-duʿāʾ, then, contributes an important chapter to the ongoing interchange between India and the eastern Islamic world. Unlike the majority of treatises which deal with India, it is written in Arabic rather than Persian, though not a few loan words are employed. I present here an edition, translation, and analysis of the relevant chapter.

New methods for centro-facial rejuvenation

New methods for centro-facial rejuvenation

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Background/Aims: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Methods: Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hyaluronic acid (HA) injection treatment (control group). Outcomes in the two groups were compared by assessing pictures taken at the same angle before and after treatment, postoperative recovery, incidence of local absorption and cysts, and skin quality before treatment, and at 1, 12, 24 months after treatment using the VISIA Skin Image Analyzer and a SOFT5.5 skin test instrument. Results: NFSCs expressed CD29, CD44, CD49d, CD73, CD90, and CD105, but did not express CD34, CD45, and CD106. NFSCs also differentiated into adipocytes, osteoblasts, and chondrocytes under appropriate induction conditions. NFSCs released large amounts of growth factors such as VEGF, bFGF, EGF, and others, and growth factor levels increased in a time-dependent manner. At the same time, PRF enhanced proliferation of NFSCs in vitro in a dose-dependent manner, and the growth curves under different concentrations of PRF all showed plateaus 6d after seeding. Facial skin texture was improved to a greater extent after combined injection of nanofat and PRF than after control injection of HA. The nanofat-PRF group had a higher satisfaction rate. Neither treatment caused any complications such as infection, anaphylaxis, or paresthesia during long-term follow-up. Conclusion: NFSCs demonstrate excellent multipotential differentiation and paracrine function, and PRF promotes proliferation of NFSCs during the early stage after seeding. Both nanofat-PRF and HA injection improve facial skin status without serious complications, but the former was associated with greater patient satisfaction, implying that nanofat-PRF injection is a safe, highly effective, and long-lasting method for skin rejuvenation.

Mesotherapy – a method of facial skin rejuvenation from an interdisciplinary perspective on improving facial aesthetics

Mesotherapy has been used in medicine since the 1950s and for aesthetic procedures since the 1970s. In the medical literature there are numerous reports about the positive effect of mesotherapy with regard to rejuvenating and improving the appearance of facial skin (reduction of fine wrinkles and discolouration, facial contour correction, improvement in skin tightness and elasticity). The variety of formulations used and the different techniques for administering them mean that the subjectively observed effects of mesotherapy cannot be objectively verified. To date, only a few studies have been published in the international literature where histopathological, ultrasound, and electron microscopy examinations were performed to confirm the efficacy of this method. Most studies, however, have been based on assessing subjective improvement. The aim of this paper was to review literature on the subject, namely 15 full-text reports on the effects of mesotherapy in the treatment of facial skin. The findings of the review show that the studies published to date are based primarily on subjective assessment methods, presenting the results of mesotherapy procedures through an analysis of "before and after" medical photographic documentation. It would seem necessary that further research should be conducted based on a unified protocol.

Short-pulsed laser for the treatment of tattoos, pigmented lesions, scars and rejuvenation.

This review describes the use of picosecond lasers for the treatment of tattoos, pigmented lesions, scars, and their use in rejuvenation. These devices have delivered enhanced efficacy for the treatment of tattoos and pigmented lesions when compared to the older 40-50 nanosecond devices. The fractional delivery with the picosecond devices have opened up a new method of rejuvenation for photodamaged skin and the treatment of scars. The delivery of these high-energy short pulses have created zones of injury in the skin referred to as areas of laser-induced optical breakdown. These areas of damage appear to produce cytokines and chemokines which result in epidermal and dermal repair and remodeling. The dual use of these devices with the flat and the fractional optics have made these devices useful in many ways that have been unanticipated.

Dynamic Model of Applied Facial Anatomy with Emphasis on Teaching of Botulinum Toxin A.

The use of botulinum toxin type A is considered one of the most revolutionary and promising face rejuvenation methods. Although rare, most of the complications secondary to the use of botulinum toxin A are technician dependent. Among the major shortcomings identified in the toxin administration education is unfamiliarity with applied anatomy. This article proposes the use of body painting as an innovative method of teaching the application of botulinum toxin A. Using the body painting technique, facial anatomy was represented on the face of a model showing the major muscle groups of botulinum toxin A targets. Photographic records and films were made for documentation of represented muscles at rest and contraction. Using the body painting technique, each of the muscles involved in facial expression and generation of hyperkinetic wrinkles can be faithfully reproduced on the model's face. The documentation of the exact position of the points of application, the distribution of the feature points in the muscular area, the proper angulation and syringe grip, as well as the correlation of the points of application with the presence of hyperkinetic wrinkles, could be properly registered, providing professional training with information of great practical importance, development of highly effective treatments, and low complication rates. By making it possible to interrelate anatomy of a function, body painting is proposed in the present study as an innovative method, which in a demonstrative and highly didactic manner presents great potential as a teaching tool in the application of botulinum toxin A.

The Fountain of Youth: A Tale of Parabiosis, Stem Cells, and Rejuvenation.

Transfusion (or drinking) of blood or of its components has been thought as a rejuvenation method since ancient times. Parabiosis, the procedure of joining two animals so that they share each others blood circulation, has revitalized the concept of blood as a putative drug. Since 2005, a number of papers have reported the anti-ageing effect of heterochronic parabiosis, which is joining an aged mouse to a young partner. The hallmark of aging is the decline of regenerative properties in most tissues, partially attributed to impaired function of stem and progenitor cells. In the parabiosis experiments, it was elegantly shown that factors derived from the young systemic environment are able to activate molecular signaling pathways in hepatic, muscle or neural stem cells of the old parabiont leading to increased tissue regeneration. Eventually, further studies have brought to identify some soluble factors in part responsible for these rejuvenating effects, including the chemokine CCL11, the growth differentiation factor 11, a member of the TGF-β superfamily, and oxytocin. The question about giving whole blood or specific factors in helping rejuvenation is open, as well as the mechanisms of action of these factors, deserving further studies to be translated into the life of (old) human beings.

Vertical Lifting: A New Optimal Thread Lifting Technique for Asians.

With the evolution of facial rejuvenation methods, thread lifts have gained popularity among patients who seek cosmetic improvement. Absorbable wedge-shaped polydioxanone (PDO) sutures are currently available, and they are extremely popular in the aesthetic clinics in Korea. In case of midface and mandibular jowl lift, threads are most often inserted in an oblique manner with vectors of rejuvenation directed toward the temple. However, specific characteristics of skeletal anatomy should be considered when deciding which technique to use in Asians. Herein, the authors introduce a vertical lifting technique most suitable for Asians, in which short (6 cm in length), wedge-shaped PDO sutures are inserted vertically downward in the anterior malar and submalar areas. To describe a novel technique adopted to counteract the descent and laxity of the Asian face. The authors also aimed to assess the overall safety and efficacy of wedge-shaped PDO thread lift in facial rejuvenation. A retrospective chart review was performed on cases of facial laxity treated with vertical thread lifting. A total of 39 Korean patients were included. All participants underwent a single treatment session. The results were assessed objectively using serial photography and subjectively based on the patients' satisfaction scores. Complications were also recorded. Most patients (89.7%) considered the results satisfactory. Consensus ratings by 2 independent dermatologists showed that the objective outcomes at the 6-month follow-up were largely categorized as very much improved (10.3%), much improved (43.6%), and improved (33.3%). The incidence of complications was low, and the complications were minor. Thread lifting with short, wedge-shaped PDO sutures is safe and effective for facial rejuvenation. The authors' vertical lifting technique has several advantages over the current approaches. First, the vectors directly oppose the vertical sagging of the face, which makes the technique highly efficient. The technique also carries a lower risk of accentuating the cheekbones, a feature which most Asians do not prefer.