Mediterranean Region Research Articles

Eastern Mediterranean Maritime Boundary Delimitation: The Claims of the State of Palestine under UNCLOS

The 2015 accession of Palestine to the United Nations Convention on the Law of the Sea (UNCLOS) has implications for the rights and obligations of Eastern Mediterranean States. Palestine’s maritime entitlements under UNCLOS are almost two and a half times the land area of Gaza in the form of a territorial sea. Palestine’s maritime sovereign rights may extend up to 200 NM offshore Gaza within the exclusive economic zone and continental shelf with tremendous economic potential. This article examines Palestine maritime claims under UNCLOS and the corresponding delimitation process. It argues that the application of UNCLOS in the East Mediterranean region may contribute to an equitable settlement of the conflict between Israel and Palestine.

Genetic Variability in Mediterranean Coastal Ecosystems: Insights into Ostrea spp. (Bivalvia: Ostreidae)

Oysters are sessile, filter-feeding bivalve molluscs widely distributed in estuarine and coastal habitats worldwide. They constitute a valuable resource for fisheries and extensive aquaculture and provide essential ecological services. Yet, their genetic diversity and distribution remain understudied. The variability in shell morphology complicates species classification, which is influenced by environmental and genetic factors. Although molecular phylogenetics research has refined oyster taxonomy and identified approximately 100 extant species, numerous taxonomic uncertainties persist. In the present study, we aimed to document the occurrence of small flat oysters of the genus Ostrea along the Mediterranean coastal areas of Liguria and Sardinia (Italy). Specifically, 16S rRNA sequence data were used to identify Ostrea species. Our findings offer novel insights into the O. stentina species complex and O. neostentina, a new species in the Mediterranean coastal areas of Italy. The study data further our understanding of Ostrea species diversity, distribution, and evolutionary patterns.

Responses to drought of two Mediterranean ring-porous, deciduous species: Searching for climate smart trees and shrubs

Drought-tolerant tree species with high growth rates and a good capacity for carbon storage in woody tissues (dense wood) are searched for due to aridification. Deciduous, ring-porous tree and shrub species could show such drought tolerance and growth traits, thus representing good candidates for climate-smart rewilding. However, we still do not know the long-term growth rates of these species and how they respond to drought, particularly in climate change hotspots such as the Mediterranean Basin. We analysed these issues at the site and individual levels in two ring-porous, deciduous species (Pistacia terebinthus, Celtis australis) using dendroecology and wood anatomy. The ring width, earlywood vessel diameter, vessel density (VD) and area (%) were measured in two focal sites, one per species, and then growth data were compared with two secondary sites to test if site-to-site synchrony changed through time. Ring-width indices (RWI) and the hydraulic diameter (Dh) of earlywood were calculated. Growth rates (ring width), Dh and vessel area were higher in C. australis (1.03−2.26 mm, 269 μm, 33.9 %) than in P. terebinthus (0.57−0.72 mm, 146 μm, 21.5 %). Consequently, VD was higher in P. terebinthus than in C. australis (104 vs. 61 vessels mm−2). The ring width and Dh were more coupled in P. terebinthus (r = 0.43) than in C. australis (r = 0.32). RWI series of the focal and secondary sites have been synchronized since the 1990s as temperatures rose. Precipitation during the growing season (May, June) enhanced growth and VD of both species. P. terebinthus was more responsive to a drought index than C. australis. The two study species show high growth rates and tolerate drought being thus suitable candidates for climate-smart rewilding.

Expanding the boundaries in the face of global warming: A lesson from genetic and ecological niche studies of Centaurium erythraea in Europe

Climate change affects plant species, especially those with restricted ecology and distribution. Centaurium erythraea is a flowering plant species in the Gentianaceae family, native to Europe, with its centre of diversity in the Mediterranean and western Asia. Of the 11 infraspecific taxa distinct from C. erythraea, only two are common in Europe: C. erythraea subsp. erythraea (widespread nominal subspecies) and C. erythraea subsp. majus (mainly distributed in the western Mediterranean region). Freshly collected samples of 36 plants from 11 localities across Lower Silesia (Central Europe) were utilised for taxonomic and genetic analysis. The barcode sequences of chloroplast DNA region matK were used for molecular analysis. Data deposited in GenBank was also used. Five haplotypes were identified among the analysed specimens. Species Distribution Modelling (SDM) techniques were applied to predict the current and future (short- and long-term projections) potential distribution of C. erythraea subsp. majus and to identify the most influential climatic factors. Despite the typical Mediterranean distribution, the presence of C. erythraea subsp. majus outside its natural range in SW Poland has been confirmed by morphological and genetic studies. The mean monthly precipitation of the wettest quarter and the mean daily temperatures of the warmest quarter were identified as the key climatic factors. Short-term scenarios suggest that C. erythraea subsp. majus will maintain most of its current suitable habitats and potentially expand into the lowlands of Central Europe. However, long-term projections indicate a potential reduction in its currently suitable areas, especially in the southern parts of its range, with a possible expansion into north-western Europe. The results of these studies provide clear evidence of the impact of ongoing climate change on species range changes. These findings suggest that climate change may create new opportunities for Mediterranean species to spread to new regions, using C. erythraea subsp. majus as an example.

Sensory Evaluation and Consumers' Acceptance of a Low Glycemic and Gluten-Free Carob-Based Bakery Product.

Carob pulp flour has antidiabetic and antioxidant activities, is naturally sweet, and is rich in fibers. It is obtained from carob pod pulp from the evergreen tree Ceratonia siliqua L., which is grown in Mediterranean areas and is known for locust bean gum production. Despite its valuable effects on health, such as the modulation of the glycemic index, this ingredient has a tremendous impact on technological and hedonic features, mainly on color, flavor, and texture. In this paper, the qualitative features and consumers' acceptance of a carob-based gluten-free bakery product where rice flour was substituted at 40% with carob pulp flour were studied. A panel group of experts described the bread as dark, quite dense, sweet, aromatic, and with a limited bubble dispersion. On the other hand, the sensory assessment and the willingness to pay of consumers were assessed in two groups (a fully informed one about heathy attributes of the food and a blind one). The results indicated a moderate appreciation of the overall quality of the product (average score between 4 and 5 points on a 9-point Likert scale). The information about the food's healthy properties and the ability to maintain a low glycemic index did not enhance the consumers' perception of the product, while previous knowledge and involvement in the product consumption were perceived to have primary importance regarding the final consumers' choice. Finally, an accelerated shelf-life test was run on the packaged snack to evaluate the general quality and stability. The protective packaging helped in limiting bread decay and maintaining the textural characteristics.

Spatiotemporal changes and background atmospheric factors associated with forest fires in Turkiye.

In this study, spatiotemporal analysis of forest fires in Turkiye was undertaken, with a specific focus on the large-scale atmospheric systems responsible for causing these fires. For this purpose, long-term variations in forest fires were classified based on the occurrence types (i.e. natural/lightning, negligence/inattention, arson, accident, unknown). The role of large-scale atmospheric circulations causing natural originated forest fires was investigated using NCEP/NCAR Reanalysis sea level pressure, and surface wind products for the selected episodes. According to the main results, Mediterranean (MeR), Aegean (AR), and Marmara (MR) regions of Turkiye are highly susceptible to forest fires. Statistically significant number of forest fires in the MeR and MR regions are associated with global warming trend of the Eastern Mediterranean Basin. In monthly distribution, forest fires frequently occur in the MeR part of Turkiye during September, August, and June months, respectively, and heat waves are responsible for forest fires in 2021. As a consequence of the extending summer Asiatic monsoon to the inner parts of Turkiye and the location of Azores surface high over Balkan Peninsula result in atmospheric blocking and associated calm weather conditions in the MeR (e.g. Mugla and Antalya provinces). When this blocking continues for a long time, southerly winds on the back slopes of the Taurus Mountains create a foehn effect, calm weather conditions and lack of moisture in the soil of Antalya and Mugla settlements trigger the formation of forest fires.

Investigating ‘Land-Use Trajectories’ in Mediterranean Rural Areas with Official Statistics and a Multiway Factor Analysis

While displaying diversified economic values, agro-ecosystems remain a relevant component of rural landscapes in Southern Europe. Coupled with the expansion of ecologically fragile areas because of climate warming, intensification and simplification of rural landscapes—e.g., in lowland—represent frequent trajectories of rural land-use change (RLUC) in the Mediterranean region and demonstrated to harm ecosystem functionality and ecological quality, especially in a context of socioeconomic transformations of landscapes. Additionally, an incipient ‘extensivation’ of geographically remote and economically marginal agricultural systems was also observed, likely following depopulation and land abandonment. The present study elaborated in this direction, providing an integrated RLUC evaluation scheme based on a multivariate analysis of land-use indicators derived from official statistics. This approach was applied to a continuous, long-term RLUC monitoring of a rural landscape in an ecologically complex Mediterranean region (Latium, Central Italy) experiencing multiple trends (e.g., lowland urbanization, crop intensification in gently sloping areas, and land abandonment/depopulation in steep zones) with diversified environmental implications. Based on administrative inventories, such evaluation was carried out over fifty years (1970–2020) in the study area, considering trends over time in selected crop surfaces at the municipal scale as inputs of a multiway factor analysis (MFA). This analysis quantifies stability in the main rural land-use types and possible changes in the dominant farming systems. Simplified indicators of land configuration (namely a crop intensity index and a rural woodland index reflecting ‘intensification’ or ‘extensivation’ of local farming systems) made available at the same spatial and temporal scales completed the informative picture corroborating MFA results. Taken together, our findings delineate multiple changes in rural landscapes, discriminating land-use trajectories in coastal and inland districts. These development trajectories were basically dependent on processes of (i) land abandonment in relict and remote areas, (ii) crop intensification in dynamic rural districts, and (iii) fringe urbanization along the coastal strip.

Extraction of Origanum onites L. using an industrial-type microwave-assisted distillation (MWAD) system: Increasing energy saving and essential oil yield compared to conventional steam distillation

Oregano species are commonly utilized for different medical purposes as well as for other essential oil products. One of the oregano species, Origanum onites L. has a dense natural distribution in the Aegean and Mediterranean regions of Turkey and Greece and is cultivated mainly in Denizli province. Turkey meets 80 % of the world's oregano market. Since Origanum onites L., whose essential oils are of high quality, has a high economic value, assessing the quality and production of essential oils extracted using various methods is important. Microwave-assisted distillation (MWAD) processes are generally carried out under laboratory conditions using clevenger or small-scale specialized instruments made for research in the lab. Thus, designing industrial-scale distillation systems with microwave power is necessary. For this reason, an industrial-type MWAD system with 12 magnetrons (power output of 1 kW) was developed in this work, and both conventional steam distillation (SD) and MWAD of oregano plant were realized, as a novelty. This study experimentally investigated the effects of microwave power and applied time on both energy saving (%) and essential oil yield (%). System was designed to distill 9 kg of dried plants chopped at once and the results obtained were used to obtain reference data for MWAD systems that can be used in industrial systems. The obtained essential oil samples were subjected to GC-MS analysis for chemical analysis. A statistical analysis of the experimental data was performed via Design Expert software to elucidate the impact of microwave power and time on essential oil yield and energy savings. When the results were evaluated, the essential oil yield of oregano plant increased by 4–22 % with the MWAD system compared to SD. The highest increase in yield was achieved with the test (500 W + 40 min). Also, the distillation time with MWAD system was 50 % shorter than with SD system. The MWAD system consumed at least 25 % less energy. According to the GC-MS analysis results, carvacrol increased steadily depending on the time applied microwave power as well as oxygenated monoterpenes. The chemical composition of the essential oils obtained from the proposed MWAD system did not show any incompatible value according to the ISO-7925 standard. As a result, this proposed method can be safely used in industrial systems.

An efficiently biological nitrogen fixation of non-native Lotus tenuis justifies its key role in the flooding Pampas (Argentina)

Forage production is a major challenge in the Salado River basin, Argentina (also denominated “Flooding Pampas”), the largest livestock raising area. The successful naturalization of the European legume Lotus tenuis (native from Mediterranean area) has been of productive and environmental relevance to the region. The main objective of this work was to quantify the efficiency of L. tenuis through its ability to fix nitrogen biologically and thus strengthening their strategic role in the region. The 15N natural abundance method was used to evaluate the Biological Nitrogen Fixation (BNF) of L. tenuis in soil plots. Non-leguminous weed species in the same plots were utilized as reference plants. Simultaneously, evaluations were carried out in the greenhouse and the relative reproducibility of the field experiments. The results showed that L. tenuis promotion was accompanied by an increase in forage quality improving the Crude Protein (CP) content and the Digestibility of Dry Matter (DDM). Moreover, its contribution to N inputs to the system through BNF in symbiosis with native rhizobia was about 80%, a highly relevant percentage for these constrained agroecosystems where the presence of native legumes is very scarce. In conclusion, this study supports the hypothesis that L. tenuis promotion plays a strategic role in ecosystem sustainability in the Salado River basin, especially in soil constrained areas. Together with previously published data, including information on ruminant management, the results obtained contribute to supporting the idea that the naturalized legume plays a strategic role in the sustainability of marginal agricultural soils.

Optimisation of Nearly Zero Energy Building Envelope for Passive Thermal Comfort in Southern Europe

The pursuit of sustainable and energy-efficient construction is vital to mitigate climate change and reduce carbon emissions. The application of the concept of nearly Zero Energy Building (nZEB) is now a reality for new buildings in the European Union, helping to achieve those goals. However, there is significant complexity in achieving acceptable thermal comfort levels in warmer climates such as the one in Southern Europe. This study carried out a multi-objective optimisation of the nZEB envelope using current construction solutions and nZEB regulations currently in force in different climate zones in this region, aiming to reduce thermal discomfort according to EN 16798-1. The results indicate that passive measures induced by regulatory requirements can significantly reduce discomfort at an affordable cost. However, great caution must be taken in relation to regulatory requirements, mainly for the cooling season, aiming to avoid summer overheating of dwellings and guaranteeing that nZEB’s buildings are sustainable and comfortable in the Mediterranean climate regions. In addition, designers should be aware that increasing the insulation layer beyond regulatory requirements does not necessarily imply an increase in passive thermal comfort. Often, this implies, in addition to an increase in construction costs, an increase in discomfort, particularly during the cooling season.

Rejuvenating exploration opportunities in Egypt's Mediterranean region using basin-scale 3D seismic reprocessing and subsurface evaluations

The Eastern Mediterranean region has proven to be a key province for natural gas resources over the last two decades. Gas discoveries in the Nile Delta Basin, Levantine Basin, and over the Eratosthenes platform have proven that an efficient working petroleum system extends to the deepwater areas and into the distal parts of the Nile Delta and Levantine basins. While the suprasalt biogenic post-Messinian play is adequately imaged in various areas in the Eastern Mediterranean region, seismic imaging of the deeper subsalt pre-Messinian plays is challenged by legacy seismic acquisition and processing techniques. Moreover, the complexity of the Messinian salt structures and the heterogeneity of the lithology of the Messinian layer across the Mediterranean basins represent a key subsurface challenge. In phase 1 of the study presented in this paper, we reprocessed 12 legacy 3D seismic data sets over 12,000 km2 and constructed a consistent basin-scale earth model from the shelf areas offshore Sinai to the deepwater areas around the Zohr carbonate platform. The data processing and imaging sequences incorporate full-waveform inversion and tomography in the earth model building process followed by a basin-to-lead evaluation to delineate exploration opportunities in phase 1. The evaluations resulted in delineating Cretaceous and Oligo-Miocene untested structures. Furthermore, potential deepwater Lower Miocene sand fairways were outlined, supported by improved regional seismic imaging and consistent seismic attributes over multiple legacy surveys. The authors of this paper expect to update the results of their work after completing the earth model building and the prestack depth migration workflows of an additional 13 legacy surveys over 35,000 km2 in phase 2 of the study.

Cenozoic Volcanic Provinces and Shallow Asthenospheric Volumes in the Circum‐Mediterranean: Evidence From Magmatic Geochemistry, Seismic Tomography, and Integrated Geophysical‐Petrological Thermochemical Inversion

AbstractWe compile an extensive catalog comprising geochemistry and ages of Cenozoic volcanic provinces in the Mediterranean region, distinguishing between three groups according to the geochemistry of magmatic rocks: intraplate (IVP), subduction‐related (SRVP), and mixed‐origin volcanic provinces (MVP; intraplate with subduction imprint). In order to relate their spatial distribution to properties of the lithosphere‐asthenosphere system, we determine temperature‐depth profiles by integrated geophysical‐petrological inversion at representative locations, using Rayleigh and Love wave phase velocities, heat flow, rock densities, and accounting for thermochemical conditions. The results confirm the occurrence of thin lithosphere (<100 km) above warm asthenosphere (>1,300°C) in areas of low shear‐wave velocities in the shallow upper mantle. Nine shallow asthenospheric volumes (SAVs) between 70 and 300 km depths are identified, forming a partly interconnected belt across the Circum‐Mediterranean. A remarkable colocation exists between the SAVs and the intraplate and mixed‐origin volcanic provinces (IMVPs). Whereas dense networks of IMVPs have formed above the SAVs, IMVPs are absent in areas of thick mantle lithosphere. Magmatic activity in IMVPs at 60–70 Ma indicates that several SAVs existed already in the Paleogene (Central European, Adriatic, Western Mediterranean, and Moesian SAVs). The formation of SAVs is related either to asthenospheric upwelling caused by slab rollback and back‐arc extension (Aegean‐Anatolian, Moesian, Pannonian, Western Mediterranean SAVs), or to thermal upwelling (Adriatic, Central European, Middle East [MEA], North African, Rhine‐Rhone SAVs), with some of the latter coupled partly with continental rifting (Central European, MEA, North African, Rhine Rhone SAVs).

Nomenclature notes on vegetation of the Salicetea purpureae Moor 1958 class in Ukraine

A brief history of the syntaxonomic studies of floodplain vegetation of the Salicetea purpureae class in Ukraine is presented and the tasks of further research are established. Our study has shown that the vegetation of the class includes more than 30 associations, which belong to 6 alliances and 2 orders. According to the ecological peculiarities, they are divided into willow shrubs on the gravel banks of streams in the montane and subalpine belts of the temperate and boreal zones of Europe and the Caucasus (Salicion eleagno-daphnoidis), willow and poplar forests of lowland and foothill rivers formed on alluvium in the nemoral zone of Europe and at high altitudes in the Mediterranean region (Salicion albae), willow shrubs on the sandy-loamy soils of the river banks of lowlands and foothills of the nemoral zone of Europe (Salicion triandrae), willow shrubs on riverine dunes in central Ukraine (Artemisio dniproicae-Salicion acutifoliae), shrubs on temporarily flooded clay soils in central Ukraine (Rubo caesi-Amorphion fruticosae). The problems of using the phytosociological nomenclature during the analysis of the Salicetea purpureae vegetation on the territory of Ukraine are highlighted. It was found that half of the syntaxa listed in Ukrainian geobotanical literature are synonyms, published invalidly, or need typification. Every syntaxon is accompanied by explanations of its non-validity with citations of the relevant articles published in the 4th edition of the International Code of Phytosociological Nomenclature. In addition to the incorrectly cited associations, two of the six alliances (Artemisio dniproicae-Salicion acutifoliae, Rubo caesii-Amorphion fruticosae) also require correction because their description was based on the invalid nomenclatural types formed by the selection of species which are invalid according to The Euro+Med PlantBase. In this paper, we aimed to analyze the studies on the vegetation of the Salicetea purpureae class on the territory of Ukraine using the Braun–Blanquet approach, determine the issues that require more detailed research in the future, and make some notes on nomenclature discordances. Such review makes it possible to determine all mismatches in phytosociological nomenclature.

Impact of Arctic Stratospheric Polar Vortex on Mediterranean Precipitation

Abstract The Mediterranean precipitation has significant implications for the local ecology and human livelihoods. This study explores the impact of the Arctic stratospheric polar vortex on the Mediterranean precipitation during the wet season (winter) using reanalysis data and large-scale ensemble experiments that are forced by anomalous stratospheric polar vortex states. Our findings reveal that total and extreme precipitation over the Mediterranean region are increased under weak polar vortex (WPV) conditions and decreased under strong polar vortex (SPV) conditions. Moisture budget suggests that the changes in vertical motion over the Mediterranean play the dominant role in precipitation changes. It is further found that the tropospheric quasi-stationary wave train propagating from Greenland to the European continent is enhanced under WPV conditions, accompanied by warming advection over the eastern Mediterranean and vorticity advection in the upper troposphere over the whole region of the Mediterranean. Consequently, the vertical motion and precipitation there are enhanced. The southward shift of the Atlantic jet stream under WPV conditions leads to enhanced moisture transport toward the Mediterranean, providing a favorable moisture source for precipitation. In addition, the background baroclinicity in the lower troposphere around the northern Mediterranean is enhanced under WPV conditions, intensifying frontogenesis and cyclonic activities, which are commonly accompanied by intensified precipitation. These results are consistent with earlier literature and suggest that the changes in the stratospheric polar vortex may be used as a potential predicting factor for Mediterranean precipitation.

Monitoring SARS-CoV-2 infection in urban and peri-urban wildlife species from Catalonia (Spain)

BackgroundHuman activities including deforestation, urbanization, and wildlife exploitation increase the risk of transmission of zoonotic diseases. Urban and peri-urban wildlife species often flourish in human-altered environments, with their survival and behavior heavily influenced by human-generated food and waste. In Catalonia, Spain, and other Mediterranean regions, species of rodents, including the house mouse (Mus musculus), black rat (Rattus rattus), Norway rat (Rattus norvegicus), as well as wild boar (Sus scrofa) are common in urban and peri-urban areas. These species host numerous infectious agents, including coronaviruses (CoVs), posing potential human health risks. During the coronavirus disease 2019 (COVID-19) pandemic, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evolved to infect previously non-susceptible species, with variants capable of infecting rodents, emphasizing their importance in surveillance studies.MethodsThe present study assessed SARS-CoV-2 presence and/or exposure in 232 rodents, 313 wild boar, and 37 Vietnamese Pot-bellied pigs in Catalonia during the pandemic period (2020–2023).ResultsAll the animals tested for acute SARS-CoV-2 infection (232 rodents and 29 wild boar) were negative. For SARS-CoV-2 exposure, 3 out of 313 (0.96%) wild boar tested positive by ELISA, while the remaining 32 rodents, 310 wild boar, and 37 Vietnamese Pot-bellied pigs were all negative. Cross-reactivity with other CoVs was predicted for ELISA-positive samples, as the 3 wild boar tested negative by the virus neutralization assay, considered as the gold standard technique.ConclusionsThe absence of SARS-CoV-2 exposure or acute infection in wild boar and rodent species supports their negligible role in viral spread or transmission during the COVID-19 pandemic in Catalonia. However, their proximity to humans and the ongoing genetic evolution of SARS-CoV-2 underline the need for continued monitoring. Surveillance of SARS-CoV-2 infection in animal species can contribute to design measures to control the emergence of new animal reservoirs or intermediate hosts that could facilitate viral spillover events.

Integrated assessment of simultaneous threshold exceedance of heat, air pollution and airborne allergenic pollen across Europe

BackgroundClimate change is expected to elevate exposure to several environmental health risk factors, including extreme environmental temperatures, air pollution and airborne allergenic pollen. Given their interconnected effects on respiratory and cardiovascular diseases, it is crucial to evaluate these exposures simultaneously. Yet, comprehensive efforts to do so remain limited. This research aims to develop an approach using modelled data, in conjunction with health-based threshold values, to assess whether, where and when there is simultaneous threshold exceedance of heat, air pollution and airborne allergenic pollen in Europe. MethodsHourly exposure data for the three stressors were sourced from three models (air pollution: LOTOS-EUROS, pollen: Copernicus Atmosphere Monitoring Service, meteorological conditions: ECMWF) for 2021 and 2022. Thresholds for each stressor that indicate the boundary of acceptable limits were based on officially established tresholds or literature recommendations. The result is a 0.1°x0.1° resolution grid (approximately 10 km x 10 km) for each stressor, with each cell representing whether exposure met or exceeded the threshold. FindingsSimultaneous threshold exceedance of air pollution and heat is occurring in various degrees throughout Europe. In the summer of 2022, the exceedances ranged from below 1% in large parts of Northern Europe to as much as 25% of the time in the Mediterranean area. An assessment of monthly threshold exceedance patterns shows a dynamic and changing co-exposure pattern across the year, which differs per region. InterpretationThis work lays out a robust approach to assess simultaneous threshold exceedances of multiple environmental health risk stressors. This approach can guide policy makers in pinpointing high-risk areas particularly vulnerable to simultaneous threshold exceedances, and develop mitigation strategies for those areas. FundingInternal funding from TNO.

The Elimination Status of Visceral Leishmaniasis in Southeast Asia Region.

Visceral leishmaniasis (VL) is caused by an intracellular parasite that is transmitted to humans by sandfly bites. It is prevalent throughout Asia, Africa, the Americas, and the Mediterranean area, where 147million people are at risk of contracting the illness. The manifestation of heterotrophic illness relies on both Leishmania implicated and the host's immunological response, ranging from asymptomatic to severe leishmaniasis with potentially lethal effects. We reviewed the literature (published till 31st December 2023) on the worldwide situation of leishmaniasis, standard and novel detection techniques, and traditional and modern treatment strategies and endeavors to eliminate VL. Moreover, epidemiological data was collected from the World Health Organization's publicly available databases. GraphPad Prism Version 8 was used to analyze and produce figures based on the epidemiological data. Diagnosis of parasites in tissues or serology is commonly employed. Diagnosis by identifying parasite DNA using molecular techniques is becoming more popular. Despite recent findings of L. donovani resistance to pentavalent antimoniate medications, it continues to be the cornerstone in the medical management of VL. Amphotericin B and its lipid formulations, injectable paromomycin, and oral miltefosine are among the new therapy options being researched. The number of reported VL cases has reduced remarkably over the last decade due to human interventions made to eliminate VL. Particularly countries from the South East Asian region have experienced momentous progress in reducing VL cases and eliminating this disease from this region. Owing to the robust elimination programs, countries such as Bangladesh has eliminated VL as a public health concern. India and Nepal are on the verge of its elimination. Rapid diagnosis, effective and inexpensive treatment, simple access to newly discovered medications, appropriate vector control, and a well-designed vaccine are all required for the elimination of this disease burden in impoverished areas of the globe.

Innovative solutions to frontier exploration in Eastern Mediterranean salt basins

The Eastern Mediterranean region is expected to hold large undiscovered natural gas resources that could address critical energy supply needs of the surrounding countries. However, exploration is challenged due to regional geologic complexities. The combination of Messinian salt structures and diapiric shales in offshore Cyprus and Egypt often causes strong attenuation of seismic energy and severe seismic imaging illumination shadows, which lead to large uncertainty regarding subsalt reservoir presence and the underlying petroleum system assessment. As part of the efforts to solve these imaging challenges, we present innovative seismic acquisition solutions to address key exploration risks such as reservoir presence. A one-sided wide-azimuth configuration consisting of one streamer vessel and one dedicated source vessel operating under the simultaneous-source mode was used to acquire data in a cost-effective way. An 8000 in3 low-frequency source was used on both vessels to provide higher energy output in the low-frequency bands of less than approximately 4 Hz. Our preliminary results show evidence of seismic image uplift due to enhanced acquisition and processing, leading to greater confidence in interpretation, which is critical for positioning wells in future drilling campaigns. This paper highlights seismic testing results undertaken to design a large exploration-scale seismic survey in the Eastern Mediterranean and early results from the execution of that program. This will help better refine optimal design criteria for future acquisition, signal processing, and imaging in order to unlock additional resources beneath complex salt provinces and identify where additional imaging needs still exist.

Short-term soil fungal community dynamics following fire in mediterranean climate-type banksia woodlands

Fire is a dominant ecosystem process in many Mediterranean climate type ecosystems, and is predicted to increase in severity and frequency, shifting away from previous regimes in many regions. Responses of flora and fauna to fire are relatively well studied, but less is known about the responses of belowground microbiota. We quantified soil fungal dynamics over the first 12–15 months after fire, focusing on attributes of the fire regime (season, interval, severity). Soil samples were collected from three sites in a threatened woodland ecosystem in southwestern Australia, a Mediterranean-type climate region. Fungal taxa were identified via high throughput sequencing of the ITS subregion and taxonomy assigned using reference databases. Richness, diversity, abundance, community composition, and functional groups were quantified. Over the post-fire sampling period, richness and diversity declined and soil fungal community composition changed significantly throughout the sampling period, with family level taxa and functional groupings experiencing the most change. Through the sampling period, an increase in saprotrophic and endophytic fungi was observed, along with a decrease in all pathogenic fungi. We found that the post-fire fungal community is quite dynamic in the first 12–15 months after fire. We found little effect of fire interval or fire season, though our inference was limited. Our work contributes to putting belowground biota into the same conceptual frameworks as aboveground taxa and serves to inform fire managers in fire-prone Mediterranean climate type regions.

Environmental DNA as Early Warning for Alien Species in Mediterranean Coastal Lagoons: Implications for Conservation and Management

Non-indigenous species (NIS) introduction notoriously threatens the Mediterranean Sea. In addition, Mediterranean coastal lagoons play a crucial role as nurseries for marine species, which new NIS arrivals can threaten. Therefore, monitoring and early warning of NIS presence is essential in preserving biodiversity. An innovative technique for rapid and accurate species identification and biodiversity screening is the application of environmental DNA (eDNA) metabarcoding. In this research, different Penaeidae (Arthropoda, Crustacea, Decapoda) NIS specimens were collected from a Mediterranean coastal lagoon after an early warning about a potentially invasive NIS arising from next-generation sequencing data. DNA barcoding of the DNA extracted from tissue samples and amplified with specifically designed primer pairs led to the recognition of Penaeus aztecus in this NATURA 2000 protected ecosystem for the first time. DNA barcoding from DNA isolated from the water where the living specimens were stored further validated the possibility of identifying P. aztecus starting from eDNA. This approach demonstrated the validity of environmental DNA analysis in the early screening of potentially invasive NIS presence in Mediterranean protected areas and ecosystems. This work describes an applicative example of the efficacy in improving the biomonitoring of lagoon ecosystems using molecular tools and it represents a guideline for the validation of eDNA metabarcoding data for the presence of potentially invasive species.