Medicinal Plant Research Research Articles

Genome-wide identification and systematic analysis of the HD-Zip gene family and its roles in response to pH in Panax ginseng Meyer

BackgroundGinseng, Panax ginseng Meyer, is a traditional herb that is immensely valuable both for human health and medicine and for medicinal plant research. The homeodomain leucine zipper (HD-Zip) gene family is a plant-specific transcription factor gene family indispensable in the regulation of plant growth and development and plant response to environmental stresses.ResultsWe identified 117 HD-Zip transcripts from the transcriptome of ginseng cv. Damaya that is widely grown in Jilin, China where approximately 60% of the world’s ginseng is produced. These transcripts were positioned to 64 loci in the ginseng genome and the ginseng HD-Zip genes were designated as PgHDZ genes. Identification of 82 and 83 PgHDZ genes from the ginseng acc. IR826 and cv. ChP genomes, respectively, indicated that the PgHDZ gene family consists of approximately 80 PgHDZ genes. Phylogenetic analysis showed that the gene family originated after Angiosperm split from Gymnosperm and before Dicots split from Monocots. The gene family was classified into four subfamilies and has dramatically diverged not only in gene structure and functionality but also in expression characteristics. Nevertheless, co-expression network analysis showed that the activities of the genes in the family remain significantly correlated, suggesting their functional correlation. Five hub PgHDZ genes were identified that might have central functions in ginseng biological processes and four of them were shown to be actively involved in plant response to environmental pH stress in ginseng.ConclusionsThe PgHDZ gene family was identified from ginseng and analyzed systematically. Five potential hub genes were identified and four of them were shown to be involved in ginseng response to environmental pH stress. The results provide new insights into the characteristics, diversity, evolution, and functionality of the PgHDZ gene family in ginseng and lay a foundation for comprehensive research of the gene family in plants.

The morphology, extractions, chemical constituents and uses of Terminalia chebula: A review

Trust on traditional medicines in the treatment of ailment is at a halt and a lot of population, especially rural population, still believes on herbs due to their easy accessibility and cost effectiveness. Due to greater attentiveness concerning significance of conventional medicine in health care, research on medicinal plants would be valuable. The plants of genus Terminalia, comprising of 250 species, are widely distributed in tropical areas of the world. Fruits of Terminalia chebula (Family: Combretaceae) commonly known as black Myroblans in English and Harad in Hindi, are widely grown in Pakistan and India among many Asian and African countries and is a popular folk medicine. T. chebula has been studied for its homeostatic, antitussive, laxative, diuretic and cardiotonic activities. This article gives a vivid account of T. chebula as a natural product and aims to (i) to refresh the importance of T. chebula to the medicinal plant researchers and (ii) to presents new information such of T. chebula.

NPASS database update 2023: quantitative natural product activity and species source database for biomedical research.

Quantitative activity and species source data of natural products (NPs) are important for drug discovery, medicinal plant research, and microbial investigations. Activity values of NPs against specific targets are useful for discovering targeted therapeutic agents and investigating the mechanism of medicinal plants. Composition/concentration values of NPs in individual species facilitate the assessments and investigations of the therapeutic quality of herbs and phenotypes of microbes. Here, we describe an update of the NPASS natural product activity and species source database previously featured in NAR. This update includes: (i) new data of ∼95 000 records of the composition/concentration values of ∼1 490 NPs/NP clusters in ∼390 species, (ii) extended data of activity values of ∼43 200 NPs against ∼7 700 targets (∼40% and ∼32% increase, respectively), (iii) extended data of ∼31 600 species sources of ∼94 400 NPs (∼26% and ∼32% increase, respectively), (iv) new species types of ∼440 co-cultured microbes and ∼420 engineered microbes, (v) new data of ∼66 600 NPs without experimental activity values but with estimated activity profiles from the established chemical similarity tool Chemical Checker, (vi) new data of the computed drug-likeness properties and the absorption, distribution, metabolism, excretion and toxicity (ADMET) properties for all NPs. NPASS update version is freely accessible at http://bidd.group/NPASS.

From single- to multi-omics: future research trends in medicinal plants.

Medicinal plants are the main source of natural metabolites with specialised pharmacological activities and have been widely examined by plant researchers. Numerous omics studies of medicinal plants have been performed to identify molecular markers of species and functional genes controlling key biological traits, as well as to understand biosynthetic pathways of bioactive metabolites and the regulatory mechanisms of environmental responses. Omics technologies have been widely applied to medicinal plants, including as taxonomics, transcriptomics, metabolomics, proteomics, genomics, pangenomics, epigenomics and mutagenomics. However, because of the complex biological regulation network, single omics usually fail to explain the specific biological phenomena. In recent years, reports of integrated multi-omics studies of medicinal plants have increased. Until now, there have few assessments of recent developments and upcoming trends in omics studies of medicinal plants. We highlight recent developments in omics research of medicinal plants, summarise the typical bioinformatics resources available for analysing omics datasets, and discuss related future directions and challenges. This information facilitates further studies of medicinal plants, refinement of current approaches and leads to new ideas.

Emerging concerns and novel potentials in medicinal and aromatic plant research

Emerging concerns and novel potentials in medicinal and aromatic plant research

Reporting guidelines for medicinal plant extracts used in pharmacological and toxicological research: ConPhyMP

Summary: Every year, the number of studies that evaluate the pharmacological effects, (clinical) efficacy or the toxicity of medicinal plant extracts is constantly increasing, but the reporting quality remains unsatisfactory. One of the main reasons is that there is a lack of detailed reporting standards for guidance. In response to this long-standing challenge, a core group of nine experts with proficiency in phytochemical analysis, including editors-in-chief of leading specialist journals, and based in different research settings globally, developed the Consensus based reporting guidelines for Phytochemical Characterisation of Medicinal Plant extracts (ConPhyMP) through a multi-staged development process. This incorporated a) a global survey among medicinal plant researchers, b) a core group, who reviewed and developed the guidelines through a Delphi process, and c) an advisory group of 20 experts, including editors of leading journals and scientific societies in medicinal plants research, who provided feedback and sanctioned the final guidelines. The ConPhyMP guidelines comprise two tables with accompanying explanatory figures. The first table provides recommendations for reporting the starting material and its initial processing, and the second table presents recommendations for conducting and reporting the analytical methods for defining the chemical profile based on the type of extracts used in the research. The group hopes that the ConPhyMP will support authors as well as peer reviewers and editors assessing these studies for publication and assist the production of evidence-based guidance of studies utilising medicinal plant extracts.

New Advances of CRISPR/Cas9 Technique and its Application in Disease Treatment and Medicinal Plants Research.

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology is widely used in disease treatment and medicinal plant improvements due to its advantages, such as easy operation, low time consumption, and high efficiency. However, potential off-target risks still exist in practical applications and need to be solved urgently. This study aimed to review the application progress of CRISPR/Cas9 technology in the field of disease treatment and medicinal agriculture in recent years. Furthermore, the study discusses the ways to reduce the off-target effect of CRISPR/Cas9 technology, providing a reference for the further application of this technology. CiteSpace software was used to analyze relevant literature data from 2013 to August 2021, and search results were retrieved from Web of Science, PubMed, and CNKI databases. In the field of disease treatment, CRISPR/Cas9 technology has great potential to cure challenging human diseases and has been widely used in drug target development, drug design, and screening. In crop breeding, CRISPR/Cas9 accelerates the improvement of crop varieties and shortens the number of years of crop breeding. By adjusting the length and GC content of sgRNA and changing the concentration of Cas9/sgRNA complex to reduce the off-target effect of CRISPR/Cas9 technology, the target genes can be manipulated more accurately. CRISPR/Cas9 technology is an indispensable and key technology in the field of disease treatment and medicinal plants. With the in-depth study of the off-target effect, CRISPR/Cas9 technology can have broader application prospects in the fields of medicine and medicinal agriculture.

Neurofuzzy logic predicts a fine-tuning metabolic reprogramming on elicited Bryophyllum PCSCs guided by salicylic acid.

Novel approaches to the characterization of medicinal plants as biofactories have lately increased in the field of biotechnology. In this work, a multifaceted approach based on plant tissue culture, metabolomics, and machine learning was applied to decipher and further characterize the biosynthesis of phenolic compounds by eliciting cell suspension cultures from medicinal plants belonging to the Bryophyllum subgenus. The application of untargeted metabolomics provided a total of 460 phenolic compounds. The biosynthesis of 164 of them was significantly modulated by elicitation. The application of neurofuzzy logic as a machine learning tool allowed for deciphering the critical factors involved in the response to elicitation, predicting their influence and interactions on plant cell growth and the biosynthesis of several polyphenols subfamilies. The results indicate that salicylic acid plays a definitive genotype-dependent role in the elicitation of Bryophyllum cell cultures, while methyl jasmonate was revealed as a secondary factor. The knowledge provided by this approach opens a wide perspective on the research of medicinal plants and facilitates their biotechnological exploitation as biofactories in the food, cosmetic and pharmaceutical fields.

Best Practice in the chemical characterisation of extracts used in pharmacological and toxicological research-The ConPhyMP-Guidelines.

Background: Research on medicinal plants and extracts derived from them differs from studies performed with single compounds. Extracts obtained from plants, algae, fungi, lichens or animals pose some unique challenges: they are multicomponent mixtures of active, partially active and inactive substances, and the activity is often not exerted on a single target. Their composition varies depending on the method of preparation and the plant materials used. This complexity and variability impact the reproducibility and interpretation of pharmacological, toxicological and clinical research. Objectives: This project develops best practice guidelines to ensure reproducibility and accurate interpretations of studies using medicinal plant extracts. The focus is on herbal extracts used in pharmacological, toxicological, and clinical/intervention research. Specifically, the consensus-based statement focuses on defining requirements for: 1) Describing the plant material/herbal substances, herbal extracts and herbal medicinal products used in these studies, and 2) Conducting and reporting the phytochemical analysis of the plant extracts used in these studies in a reproducible and transparent way. The process and methods: We developed the guidelines through the following process: 1) The distinction between the three main types of extracts (extract types A, B, and C), initially conceptualised by the lead author (MH), led the development of the project as such; 2) A survey among researchers of medicinal plants to gather global perspectives, opportunities, and overarching challenges faced in characterising medicinal plant extracts under different laboratory infrastructures. The survey responses were central to developing the guidelines and were reviewed by the core group; 3) A core group of 9 experts met monthly to develop the guidelines through a Delphi process; and. 4) The final draft guidelines, endorsed by the core group, were also distributed for feedback and approval to an extended advisory group of 20 experts, including many journal editors. Outcome: The primary outcome is the “Consensus statement on the Phytochemical Characterisation of Medicinal Plant extracts“ (ConPhyMP) which defines the best practice for reporting the starting plant materials and the chemical methods recommended for defining the chemical compositions of the plant extracts used in such studies. The checklist is intended to be an orientation for authors in medicinal plant research as well as peer reviewers and editors assessing such research for publication.

Next-generation sequencing in the biodiversity conservation of endangered medicinal plants.

Medicinal plants have been used as traditional herbal medicines in the treatment of various types of diseases. However, the increased demand for these plants highlights the importance of conservation specifically for endangered species. Significant advancements in next-generation sequencing (NGS) technologies have accelerated medicinal plant research while reducing costs and time demands. NGS systems enable high-throughput whole genome sequencing as well as direct RNA sequencing and transcriptome analysis. The sequence data sets created can be used in a variety of areas of study, including biodiversity conservation, comparative genomics, transcriptomic analysis, single cell mining, metagenomics, epigenetics, molecular marker discovery, multi genome sequencing, and so on. Commercial sequencing service providers are constantly working to improve technologies to address bioinformatics problems in NGS data analysis. Several genome sequencing projects on medicinal plants have been completed recently and a few more are in the works. In some medicinal plants, massive NGS-based data has been developed. In the present review, we have attempted to briefly discuss advancements in NGS technology on medicinally essential plants in India. The review will also provide ideas for applying NGS technologies for exploring genomes of various endangered medicinal plants whose genome sequences are not normally available and thus provides valuable insights for the conservation of these vulnerable species.

Determination of HPLC Chromatogram Profile of Katuk (Breynia androgyna (L.) Chakrab. & N.P.Balakr) Plants from Ristoja's Results using Chemometric Analysis

The katuk plant was known as Sauropus androgynous (L.) Merr, but the name is changed to Breynia androgyna (L.) Chakrab. & N.P. Balakr since the publication of Chakrab's & N.P. Balakr in the 2012 Journal of Plant Taxonomists. The content of chemical compounds and secondary metabolites of katuk leaf are strongly influenced by different habitat or location. Therefore, the Center for Research and Development of Medicinal Plants and Traditional Medicines, National Institute of Health Research and Development (NIHRD) has conducted Research on medicinal plants and herbs (RISTOJA) results on 58 samples of katuk plants originating from 7 provinces and used by 13 ethnic groups in Indonesia. This study aims to obtain plant quality based on the description of the High-Performance Liquid Chromatography (HPLC) chromatogram profile, in order to obtain the suitability of the efficacy of medicinal plants with their benefits for the community in an area. Test using HPLC with a gradient mobile phase, mixture of acetonitrile and methanol for 60 minutes. The results of the chromatogram were analyzed chemometrically by using Principal Component Analysis (PCA) data interpretation. PCA results showed that from HPLC chromatograms at 254 nm and 366 nm, each gave 3 different clusters, namely Clusters A, B and C where each cluster has the same chromatogram profile of katuk plants. Cluster A which was identified at a wavelength of 254 nm was the most used cluster by 7 ethnic groups with a total of 28 Katuk samples. Similarly, the chromatogram at a wavelength of 366 nm with the largest cluster is cluster A which is used by 11 ethnic groups with a total of 45 samples of katuk plants. where each cluster had the same chromatogram profile of katuk Plants.

Indian Medicinal Plant Research during the Last Decade (2012-2021): A Scientometric Analysis

Many published materials demonstrate the growing interest in medicinal plant research within the research community. This scientometric analysis examined Indian scientists’ medicinal plant research output from 2012 to 2021. To analyse and provide findings based on scientometric indicators, such as annual research trends, relevant sources, most productive authors and affiliations, nation’s collaboration, and funding bodies. 12,281 Scopus publications were assessed with Excel, Bibliometrix, and VOSviewer. International Journal of Pharmacy and Pharmaceutical Sciences is the primary contributing source, while 2012 was the most productive year. Among Indian authors, Bhatt I. D. was found the most prolific. The majority of the scientists published their documents as articles, the Central Institute of Medicinal and Aromatic Plants was the most active contributor, and the University Grants Commission was the major funding organisation for medicinal plant research. This kind of scientometric analysis provides insight to academicians to understand the knowledge structures in a domain.

Innovation in traditional organic nutrient management practices for better soil health and higher productivity of brahmi (Bacopa monnieri L.)

This study was conducted at Medicinal Plants Research and Development Centre (MRDC) of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, India, to examine the effect of Jeevamrit (Bioenhancer) on soil health and herbage yield of brahmi crop (var. CIM- Jagriti) and to optimise its rate of application. The experiment was laid out in randomised block design (RBD), replicated trice. The experimental soil was sandy clay loam in texture, neutral in reaction, having pH 6.9, medium in organic carbon (0.64%), low in available nitrogen (180.78 kg ha-1) and medium in both available phosphorus (20.14 kg ha-1) and potassium (200.64 kg ha-1). Jeevamrit enhances soil nutrient content, improve bulk density and biodiversity by increasing beneficial soil microbes which mineralise the nutrients present in soil and increase their availability. Jeevamrit 4000 l ha-1 can be used as a nutrient source in place of chemical fertilisers and expensive bulky organic manures under organic nutrient management.

GROWTH REGULATORS AND MICROFERTILIZER ON POLEMONIUM CАERULEUM L. APPLICATION: EFFECTIVENESS FOR MEDICINAL RAW MATERIAL AND SEEDS YIELD INCREASING

Introduction. The article presents the results of the use of growth regulators and micro-fertilizers on the medicinal culture namely Polemonium ceruleum L. according to the concept of exogenous bioregulation of growth processes, the productivity of raw materials and the content of biologically active substances in raw materials. Rhizomes with roots of this species (Rhizomata cum radicibus Polemonii) are included in the Russian State Pharmacopoeia 14th edition as an expectorant.
 The purpose of this work is to identify the possibility of increasing the yield and quality of Polemonium caeruleum medicinal plant raw materials with foliar treatment by Hardy’s retardant, Siliplant micronutrient fertilizer, Zircon growth regulator and their tank mixture under conditions of the Non-chernozem zone of the Russian Federation.
 Materials and methods. Experiments to study the effect of Siliplant microfertilizer, Zircon growth regulator, their tank mixture, and Hardy’s retardant on the growth, development and bioproductivity of Polemonium caeruleum plants was conducted in 2019-2020 in the field crop rotation at the All-Russia Medicinal Plants Research Institute (VILAR). In the second and third years of the growing season, two treatments of plants were performed with preparations at the following consumption rates: Siliplant (1 l / ha), Zircon (50 ml / ha), Siliplant (1 l / ha) + Zircon 50 ml / ha), Hardy (0, 2 l/ha), in the control plants were treated with water. Consumption of solution - 300 l / ha. The first treatment was conducted in the budding phase (2nd decade of May), the second - two weeks after the first. The quantitative content of triterpene saponins in terms of β-escin in medicinal raw materials was determined according to the Russian Farmacopeia (FS 2.5.0039.15).
 Results. Microfertilizer Siliplant and Hardy retardant increased the yield of roots and rhizomes of Polemonium caeruleum plants by 0.24 and 0.16 t/ha, respectively. The highest content of biologically active substances was observed in plants treated with Siliplant, Siliplant + Zircon and Hardy, the excess over the control was 22 %, 11 %, and 7 %, respectively, while in the variant with the growth regulator Zircon the opposite picture was observed – the content of biologically active substances on 14 % less. The tests carried out showed that all experimental options for seed yield and weight of 1000 pcs. outnumbered control. The greatest increase in seed yield was observed in the Siliplant and Hardy variants (25 and 17 %, respectively). The Siliplant + Zircon and Zircon variants showed lower efficiency – the excess was 11 and 6 %. In addition, the treatment of plants with all studied preparations is an effective method of increasing sowing qualities: energy of seed germination increases by 50-63 % and germinating capacity by 6-13 %.
 Conclusion. The results of the study are relevant for the development of environmentally friendly agricultural technologies for the cultivation of Polemonium cаeruleum medicinal crops.

The effectiveness of fermented and boiled solution of various medicinal plants in drinking as phytobiotics on broiler performance

Medicinal plants research as phytobiotics in poultry continues to be developed. Its aim to maintain body health so that it can produce good performance without relying on the use of antibiotic growth promoters which are often added to commercial animal feed. This study used various medicinal plants as phytobiotics in drinking using a CRD with 9 treatment and 3 replications. Parameters observed were feed intake (g/head), body weight gain (g/head) and feed conversion ratio. The treatment given was drinking water with: D0 (control), D1-D4 is boiled medicinal plant and D5-D8 is fermented medicinal plant of: Zingiber officinale, Curcuma longa, Allium cepa, Allium sativum, Coriandrum sativum, Kaempferia galanga, Alpinia galanga, etc. The concentration of the active ingredient as phytobiotics in drinking water treatments given was 1.06%/L of broilers daily drinking water requirement. The results showed that the giving of various medicinal plants had a very significant effect (P<0.01) on all parameters research of 5 weeks. It can conclude that the best use of medicinal plants is to use garlic (Allium sativum) boiled (D1) because it is very effective in producing the low feed conversion value andhigh body weight gain compared to others treatments.

Talinum Triangulare (Water Leaf): What a Wonderful Plant?

The importance of nutraceutical plants and the contribution of phytomedicine to the well-being of quite a large number of people and their animals worldwide has attracted interest from a variety of disciplines. Nutraceutical plants have proved to be very important in medicinal plants research and because of the bioactive compounds that these plants possess, they are useful in drug research and development. This article reviewed the bioactive constituents of Talinum triangulare; its therapeutic and nutritional importance as well as the usefulness of its bioactive compounds in agricultural settings. Bioactive components such as alkaloids, flavonoids, saponins, tannins, phlobatannin, anthraquinones, steroid, terpenes, phenols, cardiac glycoside were reported to be present in T. triangulare. T. triangulare has been implicated medically in the management of cardiovascular diseases like stroke, obesity and this was attributed to the presence of some bioactive compounds such as flavonoids and antioxidants in this plant. T. triangulare was also reported to contain some important minerals such as ß-carotene, minerals (such as calcium, potassium and magnesium), pectin, protein and vitamins. Investigations involved inclusion of T. triangulare dried powder as additive in the feed and inclusion of the leaf extract in drinking water were also reported to improve growth performance as well as immune system of both aquatic and terrestrial animals respectfully. It is therefore concluded that T. triangulare leaves can contribute significantly to the health management of Man and animal and should be recommended to be included in the daily nutritional requirement of both Man and animal.

Herbonanoceuticals: A Newer approach for Delivering Herbal Drugs-A Review

Herbs and herbal compounds are of tremendous research interest due to their numerous therapeutic applications. Several folk evidences have been documented in the formulations of the ancient world's pharmaceutical system, which have drawn researchers for scientific validation. Several herbal substances have been found and demonstrated therapeutic efficacy against pathophysiological diseases. Recently, attempts have been made to use these herbal components to synthesize nanoparticles for biomedical uses. Green synthesis is the method of creating nanoparticles using herbal/biogenic materials, and it has produced numerous metallic nanoparticles. The metal nanoparticle-herb combination may show better efficacy against different pathophysiological conditions. This review tries to put forward the different metal nanoparticles formed from different herbal resources and their role in health and diseases. Although green synthesis of nanoparticle is an emerging area of research but very few data are available regarding their physiological effects, compatibility and toxicity. This review is an effort to elaborate in detail the role of medicinally important herbs in synthesizing metal nanoparticles, their physiological compatibility and therapeutic efficacy. Further, considerations and discussions are also made on limitations (toxicity) of the green synthesis of nanoparticles along with their future prospects in health and diseases. This review opens door to a completely new dimension in medicinal plant research combining the nanotechnology with herbs i.e., Herbonanoceuticals.

Post Market Analysis of Some Neem Products Sold in Nigeria

Pharmacognosy Research,2022,14,2,211-218.DOI:10.5530/pres.14.2.30Published:April 2022Type:Original ArticleAuthors:Obi Peter Adigwe, Omolola Temitope Fatokun, Kevwe Benefit Esievo, Aliyu Adamu, Kazeem Olatunji, Jemilat Aliyu Ibrahim, and Kudirat Bolanle Mustapha Author(s) affiliations:Obi Peter Adigwe, Omolola Temitope Fatokun, Kevwe Benefit Esievo, Aliyu Adamu, Kazeem Olatunji, Jemilat Aliyu Ibrahim*, Kudirat Bolanle Mustapha Department of Medicinal Plant Research and Traditional Medicine, National Institute for Pharmaceutical Research and Traditional Medicine Idu, Industrial Area, F.C.T. Abuja, NIGERIA. Abstract:Context: The wide acceptability of herbal medicines as finished herbal products is hindered by the lack of quality control profile and total trust in the purity, safety efficacy and identity of the herbal product. Aim: The study evaluated the pharmacognostic, physicochemical, chromatographic properties and microbial load of commercially packaged neem products in Nigeria towards ascertaining its identity, content and purity. Settings and Design: Finished neem products were randomly purchased from different market sources and tested for compliance to already established standards documented by WHO, NHP, WAHP and USP. Materials and Methods: The macroscopic, microscopic, physicochemical and chromatographic properties of finished neem products were evaluated using standard procedures. Chemical-markers such as rutin and quercetin were quantified in the products using a validated HPLC method. Microbiological quality (bacterial and fungal counts) were assessed according to USP and WHO methods and guidelines. Descriptive statistics was used in the study. Results were represented in percentages and mean with respect to standard deviation where appropriate. Results: Macroscopic and microscopic evaluations showed the absence of adulterants and foreign matter and the presence of characteristic features of Azadirachta indica. Physico-chemical tests depicted varying results indicating differing sources of collection and possible soil types. Conclusion: The macro, microscopic, physicochemical, chemical and microbiological properties of neem products towards checks for identification and purity have been established. The neem products contained primarily Azadirachta indica and is considered safe for consumption. The results obtained are beneficial towards enriching current monographs of the neem products for commercialization. Key words: Pharmacognostic, Microscopy, Microbial load, Validation, HPLC. View:PDF (507.91 KB)

Top 100 most cited papers on medicinal plants research

Background: The idea of the paper is to identify trends and examine the characteristics of the top 100 cited papers on medicinal plants research. Methods: Web of Science citation database was queried to identify the citations of the top 100 most cited papers on medicinal plants research. R and Excel statistical software were employed to extract bibliographic data on various parameters such as number of authors, publication year, journal name, country of origin, research collaboration, trend of research etc. The top 100 cited papers were extracted for further statistical and visual analysed on various grounds. Results: 100 top-cited articles were identified where these papers published in 51 journals, led by Journal of Ethnopharmacology (36 papers) and other journals. All most cited papers were published during 1993 to 2020. The common areas of study were categorized as radical scavenging activities of medicinal plants, antioxidant behaviour of medicinal plants, antiviral and anti-bacterial application, use of medicinal plants in diagnosis and treatment of various diseases such diabetes and cancer etc. India and China emerged as leading nations performing medicinal plants research and greater author’s collaboration were witnessed in medicinal plants research with an overall collaboration index of 4.39.

Traditional Indian Medicinal Flowers in Floral Therapeutics: A Field Survey of Purvanchal, Uttar Pradesh

Flowers play an important role in our lives, whether medicinally or professionally, and flowers help to maintain health and a positive human relationship. The therapeutic uses of flowers utilized in Ayurveda has been attempted to be shown. Charaka Samhita, Sushruta Samhita, Ashtanga Hridaya, Bhavaprakasha Nighantu, and Pushpa-Ayurveda were used to research the therapeutic use of flowers in ancient and modern literature. In this overview, medicinal flowers are found in special Vargas (groups) of Sushruta Samhita, Bhavaprakasha Nighantu, and Pushpa-Ayurveda, respectively, and are grouped systematically in tabular form. Charaka and Vagbhat explained the medicinal advantages of flowers in a random manner. The topic is separated into two parts: the general health benefits of flowers and their pharmacological form. Most of the flowers listed in this study were used in the treatment of Jvara (Fever), Meha (Diabetes), Atisara (diarrhea), Krimi (Wormicidal), Hridaroga (In Heart problems), Kshaya (Wasting), Kasa (Cough), Svasa (Respiratorydiseases), Kustha (Skin diseases), Daha (Burning), Chardi (Vomiting), Raktapitta (Intrinsic hemorrhage), Sopha (Swelling), Dipana (Appetizer), Pachana (Digestive), and Vrana (Wound). Flowers have a special position in Ayurveda and have been employed in healing and sickness since ancient times. As a result, this work gives academics and clinicians who are actively engaged in medicinal plant research and medical practice a unique perspective.