Medical University-Sofia Research Articles

Study of pharmacy students` attitudes for scientific researches and/or academic career in Bulgaria

PURPOSE: To determine the attitudes towards participating in research projects among students at the Faculty of Pharmacy, Medical University Sofia. MATERIAL AND METHODS: A questionnaire was devised and distributed during the final compulsory project meeting to the group of pharmacy students enrolled in the scientific project in the beginning of 2012-2013 academic year. RESULTS: Among the 16 students included in the field research experiment study, 15 responded, with an overall response rate of 93.75%. Twenty percent of the students had previously participated in some form of research activity during their master degree education. Thirty three percent of all students surveyed expressed interest in participating in future research during their study and after graduation. CONCLUSION: The pharmacy students sampled reported interest in research, with a minority of the students planning to participate in extracurricular research activities during their university education, and many hoping to be involved in research throughout their future career. However, only a small number of students were interested in pursuing research through completing a PhD program.

AZITHROMYCIN DECREASE SALIVA CALPROTECTIN IN PATIENTS WITH PERIODONTAL DISEASES

Vladimir E. Panov1, Assya Krasteva2, Adriana Z. Krasteva3, Aneta Ivanova4, Anton Panov5, Zahariy Krastev4. 1) Department of Conservative dentistry and oral pathology, Faculty of Dental Medicine, Medical University Varna, Bulgaria 2) Department of Diagnostic Imaging and Oral diagnostic, Faculty of Dental medicine, Medical University, Sofia, Bulgaria 3) Department of Pharmacology and Toxicology, Faculty of Pharmacy, Medical University Sofia, Bulgaria 4) Clinic of Gastroenterology, University Hospital “St. Ivan Rilski”, Sofia, Bulgaria 5) Dentist. Private practice, Sofia, Bulgaria Journal of IMAB Annual Proceeding (Scientific Papers) 2014, vol. 20, issue 1 ISSN: 1312-773X (Online)

130th Anniversary of Prof. Dr. Vladimir Markov

This year marks 130 years since the birth of the founder and leader (untill 1958) of the Department of Microbiology at the Medical University Sofia in Bulgaria, the eminent and internationally recognized scholar Prof. Dr. VLADIMIR NESTOROV MARKOV, Full Member of the Bulgarian Academy of Sciences (Academician). ...

Genomic Markers for Glioblastoma Multiforme, Revealed by Array CGH Analysis

Aims: Despite of intensive studies of glioblastoma multiforme , still no unified concept for the most important specific molecular alterations exists for this tumor type. The method of array CGH has great potential for molecular characterization of glioblastoma. The aim of our study was to determine the type, frequen cy and fine mapping of unbalanced genomic changes and to suggest candidate genes for the emergence and development of brain tumors. Study Design: Ten tumor samples were collected from patients with glioblastoma multiforme after taking informed consent. Hi stological examination was done to confirm the presence of tumor cells in more than 75% of the samples. DNA was isolated from each tumor sample. Place and Duration of Study: The material was collected in Department of Neurosurgery (Medical University Sofia) and processed for analysis in Department of

Application of DNA Fragment Analysis in Disputed Paternity (Maternity) and Identification of the Individual

ABSTRACTThis study is based on the data from expertise and research on disputed parentage and identification of individuals carried out at the laboratory for DNA analysis in the Department of Forensic Medicine and Deontology (DFMD) at the Medical Faculty of the Medical University—Sofia over a period of thirteen years (1998–2010). The results are compared with international research on the topic.During the period 1998–2010 we worked on 680 cases for determining parentage, paternity and maternity. In 122 cases the tests concerned the exclusion of paternity (17.99%) based on the presence in the child's genotype of alleles that cannot have been inherited from the alleged biological father.The presented data give us the opportunity to lay the foundations of a broader research concerning the parentage issues. For this purpose it is necessary to examine many other factors such as age, socio-economic status of respondents, social policy, behavioral manifestations in ethnic groups, etc.

In vivo effects of Diosgenin, isolated from Asparagus officinalis, compared to Phenobarbital in rats

Event Abstract Back to Event In vivo effects of Diosgenin, isolated from Asparagus officinalis, compared to Phenobarbital in rats Magdalena Kondeva-Burdina1*, Mitka Mitcheva1, Ilina Krasteva2 and Stefan Nikolov2 1 Medical University-Sofia, Department of Pharmacology, Pharmacotherapy and Toxicology, Bulgaria 2 Medical University-Sofia, Department of Pharmacognosy and Botany, Bulgaria Diosgenin is a well-known antihypercholesterolemic agent. Biliary cholesterol secretion is mediated by different transporters and is stimulated by cholesterol and non-cholesterol steroids – Cholate and Diosgenin. It is known that nuclear receptors CAR and PXR take part in the regulation of hepatic drug metabolism. There are data that Diosgenin increased the expression of lipid-sensing nuclear receptor Pregnane X Receptor (PXR). Phenobarbital (PB) and Phenytoin are the most well known activators of PXR and CAR. PXR has been shown to take part in the regulation of hepatic metabolism – especially biochemically and genetically activation of CYP3A genes. According to these data, it is interesting to investigate in vivo the possible effect of Diosgenin, isolated from Asparagus officinalis, on the activity of some drug-metabolizing enzyme systems, compared to Phenobarbital in rats. Rats were treated with Diosgenin (50 mg/kg p.o./6 days) and Phenobarbital (75 mg/kg i.p./4 days). The total quantity of Cytochrome P450, enzyme activity of Ethylmorphine N-demethylase (EMND) (CYP3A) and Aniline Hydroxilase (AH) (CYP2E1) were measured spectrophotometrically in isolated rat microsomes. As marker of lipid peroxidation and cell protection, we measured the quantity of malondialdehyde (MDA) and reduced glutathione (GSH). Diosgenin and Phenobarbital, administered alone, increased statistically significant, compared to control, the total amount of Cytochrome P450 – with 36% and with 85%, respectively; the activity of EMND with 58% and with 184%, respectively. Diosgenin didn’t influenced the AH activity compared to Phenobarbital. Both Diosgenin and Phenobarbital have no statistically significant effect on GSH level. On the quantity of MDA, Diosgenin has no effect, while Phenobarbital increased it with 27%. Our results showed that Diosgenin, isolated from Astragalus officinalis, exhibited inducible effect similar to the effect of Phenobarbital on the activity of some drug-metabolizing enzyme systems. Keywords: Diosgenin, Phenobarbital, induction, drug-metabolizing enzymes Conference: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010. Presentation Type: Poster Topic: Xenobiotic metabolism Citation: Kondeva-Burdina M, Mitcheva M, Krasteva I and Nikolov S (2010). In vivo effects of Diosgenin, isolated from Asparagus officinalis, compared to Phenobarbital in rats. Front. Pharmacol. Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010. doi: 10.3389/conf.fphar.2010.60.00145 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Oct 2010; Published Online: 04 Nov 2010. * Correspondence: Dr. Magdalena Kondeva-Burdina, Medical University-Sofia, Department of Pharmacology, Pharmacotherapy and Toxicology, Sofia, Bulgaria, magdalenakondeva@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Magdalena Kondeva-Burdina Mitka Mitcheva Ilina Krasteva Stefan Nikolov Google Magdalena Kondeva-Burdina Mitka Mitcheva Ilina Krasteva Stefan Nikolov Google Scholar Magdalena Kondeva-Burdina Mitka Mitcheva Ilina Krasteva Stefan Nikolov PubMed Magdalena Kondeva-Burdina Mitka Mitcheva Ilina Krasteva Stefan Nikolov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Preventive effect of polyphenols from geranium sanguineum l on hepatic drug metabolism in influenza infected mice.

Event Abstract Back to Event Preventive effect of polyphenols from geranium sanguineum l on hepatic drug metabolism in influenza infected mice. Lyubka Tantcheva1, Eleonora Encheva2*, Silvyia Abarova2, Julia Serkedjieva3, Iliana Dimova1 and Elitza Pavlova4 1 Institute of Neurobiology, Bulgaria 2 Medical University-Sofia, Bulgaria 3 Bulgarian Academy of Sciences, Institute of Microbiology, Bulgaria 4 St Kliment Ohridsky Sofia University, Bulgaria Introduction: Inhibited first phase of drug metabolism is a main reason for numerous side effects and increased drug toxicity, observed in the course of influenza virus infection (IVI). Medical plant Geranium Sanguineum L. (GSL), spread in the Balkans, demonstrated significant antioxidant and antiviral activity. The aim of the study is to evaluate the preventive effect of polyphenol complex (PPC) from GSL on the oxidative drug metabolism in IVI. Methods: The experimental model of IVI (A/Aichi/2/68/(H3N2)(4.5 lg LD50) is developed in male albino ICR mice. PPC is applied nasally (10 mg/kg). In liver 9 000 g supernatant thiobarbituric acid reactive substances (TBARS) and antioxidant total activity (ATA) as well as monooxygenase N-demethylase activity (ethylmorphyne, amidopyrine and analgin) and hydroxylase activity (aniline and p-nitrophenol), NADPH- cytochrtome P-450 reductase and cytochrome P-450 content are determined. Student-Fisher t-test and correlation coefficients are used for statistics. Results: High correlation between increased TBARS and decreased monooxygenase activity (p< 0.001) is found in infected animals. It suggests that activation of free radicals can be one of the main reasons for unspecific monooxygenase inhibition, because all components of cytochrome P-450 system are inhibited (mostly the on the 6th and 9th days). PPC pretreatment demonstrates significant preventive effect against increased levels of TBRS and decreased ATA. Drug metabolism in IVI mice is restored by PPC near to healthy controls. Conclusion: TBARS probably play important role not only in the pathogenesis of IVI, but also in modulation of hepatic monooxygenase activity. All inhibited P-450 monooxygenase activities are significantly restored by PPC pretreatment. The complex mechanism of PPC-protective effect obviously combines several biological activities- antioxidant activity, selective antiviral and protein-bounding effect. In contrast to infected animals, PPC has medium pro-oxidant and weak inhibiting effect on monooxygenases in healthy animals, probably related to its protein–binding effect on membrane proteins and enzymes. Keywords: influenza infection, drug metabolism, cyt P-450 monooxygenases, Polyphenols, Geranium Sanguineum L. Conference: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010. Presentation Type: Poster Topic: Xenobiotic metabolism Citation: Tantcheva L, Encheva E, Abarova S, Serkedjieva J, Dimova I and Pavlova E (2010). Preventive effect of polyphenols from geranium sanguineum l on hepatic drug metabolism in influenza infected mice.. Front. Pharmacol. Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010. doi: 10.3389/conf.fphar.2010.60.00203 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Oct 2010; Published Online: 04 Nov 2010. * Correspondence: Dr. Eleonora Encheva, Medical University-Sofia, Sofia, Bulgaria, eleonora.encheva@medfac.acad.bg Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lyubka Tantcheva Eleonora Encheva Silvyia Abarova Julia Serkedjieva Iliana Dimova Elitza Pavlova Google Lyubka Tantcheva Eleonora Encheva Silvyia Abarova Julia Serkedjieva Iliana Dimova Elitza Pavlova Google Scholar Lyubka Tantcheva Eleonora Encheva Silvyia Abarova Julia Serkedjieva Iliana Dimova Elitza Pavlova PubMed Lyubka Tantcheva Eleonora Encheva Silvyia Abarova Julia Serkedjieva Iliana Dimova Elitza Pavlova Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Effects after multiple administration of alcohol on some biochemical parameters in liver and brain in spontaneously hypertensive rats (SHR)

Event Abstract Back to Event Effects after multiple administration of alcohol on some biochemical parameters in liver and brain in spontaneously hypertensive rats (SHR) Rumyana Simeonova1, Vessela Vitcheva1* and Mitka Mitcheva1 1 Medical University-Sofia, Departement of Pharmacology, Pharmacotherapy and Toxicology, Bulgaria Introduction. Spontaneously hypertensive rats (SHR) are a genetic model of essential hypertension in human. They are used from many laboratories to characterize some aspects of this pathology, including an influence of some toxic compounds, including alcohol. The aim of the following study was to investigate the effects of multiple alcohol administration on some biochemical brain and hepatic parameters in SHRs, compared to normotensive Wistar-Kyoto rats (WKY). Methods. The parameters: malondialdehyde (MDA), reduced glutathione (GSH) and neuronal nitric oxide synthase (nNOS) were determined in livers and brains from both strains spectrometrically. For the experiment male SHR (strain Okamoto-Aoki, n=12) and WKY (n=12), were used. The animals of each strain were divided into 2 groups: control group and group treated with alcohol (3g/ kg, p.o., 14 days). Results: GSH level was lower in the liver (29%) and in the brain (35%) of SHR, compared to the NTRs, and ethanol administration decreased it in both strains in investigated organs. MDA level was increased in SHR significantly in liver by 38% and in brain by 27% and ethanol administration enhance this parameter in both strains, in both organs, but more pronounced in NTR. nNOS activity was significantly increased in SHR by 31%, compared to NTR and alcohol didn’t affect this parameter in SHR. Conclusions. The difference in the effect of ethanol between the strains might be due to the changed reactivity and pathophysiological characteristics of the hypertensive model. Keywords: alcohol, Ethanol, Malondialdehyde, Glutathione, nNOS, hypertensive models Conference: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010. Presentation Type: Poster Topic: Xenobiotic toxicity Citation: Simeonova R, Vitcheva V and Mitcheva M (2010). Effects after multiple administration of alcohol on some biochemical parameters in liver and brain in spontaneously hypertensive rats (SHR). Front. Pharmacol. Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010. doi: 10.3389/conf.fphar.2010.60.00197 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Oct 2010; Published Online: 04 Nov 2010. * Correspondence: Dr. Vessela Vitcheva, Medical University-Sofia, Departement of Pharmacology, Pharmacotherapy and Toxicology, Sofia, Bulgaria, vesselavitcheva@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rumyana Simeonova Vessela Vitcheva Mitka Mitcheva Google Rumyana Simeonova Vessela Vitcheva Mitka Mitcheva Google Scholar Rumyana Simeonova Vessela Vitcheva Mitka Mitcheva PubMed Rumyana Simeonova Vessela Vitcheva Mitka Mitcheva Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Lichenoid Reactions Due to Dental Restorative Materials

ABSTRACTThe pathogenic relationship between the oral lichenoid reaction and dental restorative materials has been confirmed many times. Lichen planus-like lesions (2) (oral lichenoid lesion, oral lichenoid reaction) can be caused by hypersensitivity to materials for dental restorations.The aim of this study is to determine the effect of restorative dental materials removal on the lichenoid reaction development. In this case report we present two male patients who were referred to the Department of Oral Diagnostics and Maxillofacial Radiology, at the Faculty of Dental Medicine, Medical University Sofia. Both patients had lesions located on the tongue and buccal mucosa. The routine examinations together with patch tests for dental allergy were used. The patients showed hypersensitivity to Nickel (Ni), Cobalt (Co), Amalgam and Mercury (Hg). Removal of the positively reacting materials followed by new hypoallergenic restorations led to spontaneous healing.The results have shown that the removal and replacement of these restorative materials do lead to remission of the symptoms and full healing of the oral lichenoid lesions. Close association between the lesion development and the dental materials which gave positive patch tests reaction was confirmed. A replacement of the problem causing restorations with hypoallergenic materials proved to be the reliable method for controlling the clinical symptoms and also led to a state of total healing of these two patients.