Abstract We report an investigation of the inverse spin Hall effect (ISHE), as a measure of the pure spin current (JS), induced by spin pumping in a Fe65Co35/Pt bilayer using coplanar waveguide ferromagnetic resonance (CPW-FMR) in the in-plane (IP) geometry over the broadband microwave frequency range of 8-40 GHz. From the IP-FMR measurements, the defect-induced two-magnon scattering (TMS) contribution is evaluated by analyzing the frequency dependence of FMR linewidth using Arias and Mills approach. Here, we have determined a very low Gilbert damping constant (αG) for Fe65Co35 bare film around 1.3 x 10−3, which is essential for the high spin current (> 107 A/m2) generation. The enhanced value of αG ≈ 3.2 x 10−3 obtained for Fe65Co35/Pt bilayer film is due to spin pumping damping, αSP = 1.9 x 10−3, confirmed by the ISHE induced DC voltage (VDC) signal measured across the edges of Pt layer. Out-of-plane (OP) angular ISHE measurements have been performed to disentangle spin pumping from spin rectification effects in the VDC signal. Further, we evaluated the effective spin-mixing conductance geff ↑↓ = 3.33 x 1019 m-2, which is higher than the values reported in FeCo-alloy-based bilayer systems. The present study aims to detect the JS injected by low-damping Fe65Co35 thin films, leading to the realization of energy-efficient spintronic devices.