As one of the mainstream girder types of long-span bridges, the flat steel box girder (FSBG) under thermal action has been one primary concern for bridge engineers and researchers. This research presents a field study of the temperature field distribution characteristics of FSBG and its influential environmental factors. To achieve this, an acquisition equipment that comprises of thermometers and environmental sensors is applied to a prototype cable-stayed bridge under construction, in which the structural temperature field of FSBG and the surrounding environmental data are collected and analyzed thoroughly. The results indicate that the vertical temperature distribution (VTD) in certain time instants exhibits significant variations and the maximum vertical temperature gradient can reach up to 27.9 °C. Despite of this, it is found that the VTD of FSBG can be covered by several current standards such as AASHTO standard and New Zealand standard. As for the transverse temperature distribution (TTD), it is found that both the variation and temperature difference of TTD in certain time instants are considerable, especially at the deck surface, which far exceed those of the TTD prescribed in current standards. This indicates that the TTD of FSBG should be seriously considered during the bridge design phase. Furthermore, the field study reveals that the wind speed has negative effect on the structural temperature of FSBG (when the air temperature is less than that of FSBG), while the solar radiation exerts positive effect on the structural temperature of FSBG. The outcome of this study can provide valuable references for thermal design, monitoring and control of long-span bridges employing FSBGs as bridge main girder.