Statement of the ProblemBone regeneration with cultured osteoblastic cells is a new treatment option of maxillofacial reconstructive surgery. However, it is a complicated method containing unresolved problems as for cells, scaffolds and conditions. In this study, we examined osteogenesis in rat by various combinations of cell and scaffold.Materials and MethodsUntreated cultured rat bone marrow stromal cells were grafted to six-week-old Lewis rats subdermally with or without titanium mesh tray. 107 of cells were mixed with each 100 mg of atelocollagen (Integran(R)), βTCP (Osferion(R)) or hydroxyapatite (Neobone(R)) as scaffold and filled up in cylindrical titanium mesh tray of 5 mm diameter and 10 mm long, respectively. In the same manner, mesenchymal cells with treatment of induction to osteoblast were used. Each mixture was transplated to rat back subcutis. After 6 weeks, implants were resected and examined histologically.Method of Data AnalysisRegenerated bone area was examined on the section by using of image J(R).ResultsIn the stromal cell group with titanium mesh tray, significant bone formation was found in each scaffold as well as osteoblast group. On the other hand, in the control group without titanium mesh tray, bone formation was not found in each scaffold.ConclusionIt is thought that enough bone quantity formation is possible by using bone marrow stromal cell without derivation to osteoblast. However, maintenance of space for bone formation is indispensable for osteogenesis. Statement of the ProblemBone regeneration with cultured osteoblastic cells is a new treatment option of maxillofacial reconstructive surgery. However, it is a complicated method containing unresolved problems as for cells, scaffolds and conditions. In this study, we examined osteogenesis in rat by various combinations of cell and scaffold. Bone regeneration with cultured osteoblastic cells is a new treatment option of maxillofacial reconstructive surgery. However, it is a complicated method containing unresolved problems as for cells, scaffolds and conditions. In this study, we examined osteogenesis in rat by various combinations of cell and scaffold. Materials and MethodsUntreated cultured rat bone marrow stromal cells were grafted to six-week-old Lewis rats subdermally with or without titanium mesh tray. 107 of cells were mixed with each 100 mg of atelocollagen (Integran(R)), βTCP (Osferion(R)) or hydroxyapatite (Neobone(R)) as scaffold and filled up in cylindrical titanium mesh tray of 5 mm diameter and 10 mm long, respectively. In the same manner, mesenchymal cells with treatment of induction to osteoblast were used. Each mixture was transplated to rat back subcutis. After 6 weeks, implants were resected and examined histologically. Untreated cultured rat bone marrow stromal cells were grafted to six-week-old Lewis rats subdermally with or without titanium mesh tray. 107 of cells were mixed with each 100 mg of atelocollagen (Integran(R)), βTCP (Osferion(R)) or hydroxyapatite (Neobone(R)) as scaffold and filled up in cylindrical titanium mesh tray of 5 mm diameter and 10 mm long, respectively. In the same manner, mesenchymal cells with treatment of induction to osteoblast were used. Each mixture was transplated to rat back subcutis. After 6 weeks, implants were resected and examined histologically. Method of Data AnalysisRegenerated bone area was examined on the section by using of image J(R). Regenerated bone area was examined on the section by using of image J(R). ResultsIn the stromal cell group with titanium mesh tray, significant bone formation was found in each scaffold as well as osteoblast group. On the other hand, in the control group without titanium mesh tray, bone formation was not found in each scaffold. In the stromal cell group with titanium mesh tray, significant bone formation was found in each scaffold as well as osteoblast group. On the other hand, in the control group without titanium mesh tray, bone formation was not found in each scaffold. ConclusionIt is thought that enough bone quantity formation is possible by using bone marrow stromal cell without derivation to osteoblast. However, maintenance of space for bone formation is indispensable for osteogenesis. It is thought that enough bone quantity formation is possible by using bone marrow stromal cell without derivation to osteoblast. However, maintenance of space for bone formation is indispensable for osteogenesis.