The aim of the study was to evaluate the effects of nutritional intervention on body composition and hormonal profile in a group of obese patients after a weight loss of 10%. In particular, the study focused on the evaluation of aromatase activity, calculated as the ratio between total testosterone and estradiol. 28 patients aged between 18 and 65 were enrolled, fat percentage was > 30% and it was estimated with total body densitometry. Body composition (anthropometry, circumferences, DEXA), hormonal profile, cardiovascular risk indices and the HOMA index were evaluated. The patients underwent two tests: Yale Food Addiction Scale (YFAS) and Aging Male Symptoms (AMS), respectively for the assessment of carbohydrate dependence and symptoms related to hypogonadism. The nutritional treatment was based on hypocaloric, high-protein diet (1.5 g/kg ideal body weight/day). Diet energy gap was between 170–250 kcal/day. After a mean time of 6 months and a mean weight loss of 10% from baseline, a significant increase in serum total testosterone levels (405.3 ± 124.8 vs 298.2 ± 81.4 ng / dl, p = 0.002) and a significant reduction of estradiol (37.8 ± 16.2 vs 46.4 ± 12.1 pg / ml, p = 0.049) were observed. There was also a significant reduction in the percentage of total (36.1 ± 4.7 vs 39.8 ± 6.9, p = 0.0001) and segmental fat mass (android fat mass 46.3 ± 6.5% vs 52.1 ± 6.3% P = 0.0012; gynoid fat mass 36.4 ± 5.9 % vs 39.1 ± 6.1% p = 0.0001). The lean mass remained unchanged. The reduction in aromatase activity (expressed as the total testosterone / estradiol ratio) was statistically significant (110.9 ± 52.8 vs 69.1 ± 34.1, p = 0.003). Food addiction has not improved after the nutritional intervention and probiotic supplementation.
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