Abstract
IntroductionMatrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites.ObjectivesA method to keep the shape of plant tissue needs to be developed for MALDI−IMS.MethodsThe method was developed using transfer tape and double-sided conductive tape.ResultsMALDI−IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features.ConclusionThis proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis.
Highlights
Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites
To determine which matrix reagent is appropriate for detecting wide range of metabolites, we first performed the screening of matrix reagents in 36 metabolites that belongs to 12 metabolite types using four matrix reagents, including α-cyano-4-hydroxycinnamic acid (CHCA), 1,5-diaminonaphthalene, dihydroxybenzoic acid (DHB), and 3-hydroxypicolinic acid, via MALDI−MS analysis (Supplementary Methods and Supplementary Table 2)
The results suggest that CHCA and Keeping the shape of plant tissue for visualizing metabolite features in segmentation and
Summary
Specialized metabolites (previously called secondary metabolites) are significant natural products that are associated with certain species and accumulate in specific tissues and organs of plants. Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites and is used in sections of organisms (Dong et al 2016; Fujimura and Miura 2014; Lee et al 2012; Sarabia et al 2018; Sturtevant et al 2016). This approach has been applied to identify the localization of specialized metabolites in plants (Enomoto et al 2018; Jarvis et al 2017; Li et al 2014; Shiono et al 2017).
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