Agricultural practices such as the application of organic and inorganic fertilizers play a crucial role in enhancing crop productivity. The impact of poultry manure and NPK (15:15:15) fertilizer on the growth and yield of tomato (Lycopersicon esculentum L.) was examined in Owo, South Western Nigeria. This study employed a randomized complete block design (RCBD) with three treatments (No manure as control, NPK (15:15:15) at 200 kg/ha, and poultry manure at 2.5 t/ha) allocated randomly across plots using balloting methods, each replicated thrice. Seedlings were spaced at 30 x 60 cm, resulting in a total of 108 stands across nine plots. Various parameters including plant height, stem girth, number of leaves, number of flowers, number of fruits, and fruit weight were assessed through statistical analysis. Results showed that poultry manure significantly enhanced vegetative growth parameters such as plant height, leaf number, and leaf area compared to the control. At 2, 4, and 6 weeks after transplanting (WAT), poultry manure consistently outperformed NPK in terms of plant height and leaf area. However, NPK showed a superior number of flowers and fruit yield, highlighting its rapid nutrient release and immediate availability. Despite NPK's effectiveness, the overall yield attributes of poultry manure were comparable, suggesting its potential as a sustainable alternative due to its soil-enriching properties and long-term benefits. This study underscores the importance of integrating organic and inorganic fertilizers to optimize tomato yield and soil health, advocating for further research into the economic and long-term impacts of organic fertilization in tropical agriculture.