The Southeast (SE) Asian tin belt is a major tin producer globally, with a prolonged mineralization history from the Permian to Paleogene (ca. 285−45 Ma). Tin deposits in this region are typically associated with tectonic settings that involve subduction and collision of the Paleo-, Meso-, and Neo-Tethys slabs. Ca. 40 Ma, a notable transition occurred in the tectonic regime of SE Tibet, with the Neo-Tethys subduction giving way to lateral extrusion of the Indochina block along major strike-slip faults. Previously, it was believed that this shift had brought tin mineralization to a halt. In this study, we present in situ laser ablation−inductively coupled plasma−mass spectrometry U-Pb cassiterite ages of 21−19 Ma from the Yunling tin deposit located in western Yunnan, China. Yunling produces gem-quality cassiterite that is transparent but contains low U contents, which renders usual U-Pb dating techniques unusable. To address this, a customized dating protocol involving cathodoluminescence (CL) imaging and testing of U distribution on crystallographically well-defined cassiterite crystals was applied. The study revealed two types of primary microstructures in cassiterite: volumetrically dominant concentric oscillatory growth zones and subordinate CL-dark sector zones. The U content shows a preferential enrichment in the CL-dark sector zones, typically tens of parts per million (ppm), which is two orders of magnitude greater than the U content in the concentric zone. This is significant, as the dating results (21−19 Ma) obtained through the targeted selection of CL-dark sector zones represent the youngest tin mineralization event in the SE Asian tin belt. Additionally, these results challenge the established belief that the ore-hosting Indosinian granite, dated to ca. 215 Ma, was related to Sn mineralization in the Yunling area. We suggest that emplacement of the early Neogene Sn mineralization at Yunling may be genetically connected to extensive delamination of the lithosphere in southeastern Tibet. The lithospheric delamination led to the upwelling and decompression partial melting of the asthenosphere, which provides a plausible explanation for the high temperature required for the release of Sn from biotite-dehydration melting of sedimentary protolith. The study also highlights the potential of Oligocene−Miocene-aged granites situated in the Sanjiang Tethys and adjacent areas of western Yunnan as prospective exploration targets for tin mineralization.