Aim: The aim of this in-vitro study will be to evaluate and compare the accuracy of two apex locators in the presence of various irrigants. Methods and Material: The study will be conducted on 80 extracted straight, single-rooted permanent human teeth with mature apices. The teeth will be decoronated. Access cavity preparations will be done and working length will be established with 10 K file or 15 k-file into the canal until its tip emerged through the major apical foramen at ×10 magnification under a stereomicroscope. All the samples selected will be divided into four groups on the basis of irrigants used. Group 1 will be dry without irrigants, used as control, in Group 2 saline will be used as irrigant, in Group 3 sodium hypochlorite will be used as irrigant and in Group 4 chlorehexidine will be used as irrigant. Each group again will be subdivided into two groups on the basis of two different apex locators used (Root ZX Mini J. Morita) and CanalPro (Coltene). All the samples will be embedded in gelatin filled polyurethane bottles to simulate the periodontium. Statistical analysis used: Subsequently, the data will be statistically analysed using one-way analysis of variance (ANOVA) and paired t-test. Results: In this study, group 2 canal pro apex locator is accurate and sodium hypochlorite irrigant shows maximum variation. Conclusions: Canalpro showed accurate values than Root ZX and sodium hypochlorite showed more variations
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