Repair Maintenance Research Articles

M6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region.

The N6-methyladenosine (m6 A) RNA modification serves crucial functions in RNA metabolism; however, the molecular mechanisms underlying the regulation of m6 A are not well understood. Here, we establish arginine methylation of METTL14, a component of the m6 A methyltransferase complex, as a novel pathway that controls m6 A deposition in mammalian cells. Specifically, protein arginine methyltransferase 1 (PRMT1) interacts with, and methylates the intrinsically disordered C terminus of METTL14, which promotes its interaction with RNA substrates, enhances its RNA methylation activity, and is crucial for its interaction with RNA polymerase II (RNAPII). Mouse embryonic stem cells (mESCs) expressing arginine methylation-deficient METTL14 exhibit significantly reduced global m6 A levels. Transcriptome-wide m6 A analysis identified 1,701 METTL14 arginine methylation-dependent m6 A sites located in 1,290 genes involved in various cellular processes, including stem cell maintenance and DNA repair. These arginine methylation-dependent m6 A sites are associated with enhanced translation of genes essential for the repair of DNA interstrand crosslinks; thus, METTL14 arginine methylation-deficient mESCs are hypersensitive to DNA crosslinking agents. Collectively, these findings reveal important aspects of m6 A regulation and new functions of arginine methylation in RNA metabolism.

Analisis Kelayakan Pembangunan Fasilitas Vulkanisir Ban Pesawat Terbang Di Indonesia Dan Strategi Pengembangannya

Airplane tire retreading is the process of reconditioning an airplane tire layer after several take- offs and landing times. Airplane tire retreading can be done between 3-5 times depending on the wear rate of the tire layers.The total number of aircraft operating in Indonesia is 1259 according to data from the DKPPU, and data from INACA 'Indonesia Aviation Outlook 2017' the number of Wide Body jets is 36 and Narrow Body is 414. From these data, the tires with the largest population and the same size is the aircraft tires B737-800/900/ER and A320-200/300 with the number of tires on the Nose Landing Gear/NLG are 806 EA and in the Main Landing Gear/MLG are 1612 EA. The available information states that the need for retreading tires for all Jet aircraft in Indonesia is around USD 13 million per year. About 80% or around USD 10.4 million per year is spending on retreading aircraft tires for B737-800/900/ER and A320- 200/300.This figure is a big opportunity for Indonesia MRO However, MRO (Maintenance Repair & Overhaul) Indonesia cannot take this opportunity because it still does not have the ability to retread airplane tires. For this reason, the authors conducted a feasibility analysis for the construction of aircraft tire retreading facilities for the B737-800/900/ER and A320-200/300 aircraft in Indonesia and conducted a strategic analysis necessary for the construction of the tire retreading facility to be successful. From the feasibility calculation, it is obtained 29.6% ROI (Return On Investment) and 4.8 year PBP (Pay Back Period) shows that the construction of the B737-800/900/ER and A320-200/300 aircraft tire retreading facilities is very feasible. Several strategies to achieve this are collaborating with Original Equipment Manufacturer/ OEM, seeking strategic partners, asking for support from the Government (the Ministry of Industry) by submitting the tire retreading facility development to the Aerospace Industry Roadmap.

Vascular Endothelial Growth Factor: A Translational View in Oral Non-Communicable Diseases.

Vascular endothelial growth factors (VEGFs) are vital regulators of angiogenesis that are expressed in response to soluble mediators, such as cytokines and growth factors. Their physiologic functions include blood vessel formation, regulation of vascular permeability, stem cell and monocyte/macrophage recruitment and maintenance of bone homeostasis and repair. In addition, angiogenesis plays a pivotal role in chronic pathologic conditions, such as tumorigenesis, inflammatory immune diseases and bone loss. According to their prevalence, morbidity and mortality, inflammatory diseases affecting periodontal tissues and oral cancer are relevant non-communicable diseases. Whereas oral squamous cell carcinoma (OSCC) is considered one of the most common cancers worldwide, destructive inflammatory periodontal diseases, on the other hand, are amongst the most prevalent chronic inflammatory conditions affecting humans and also represent the main cause of tooth loss in adults. In the recent years, while knowledge regarding the role of VEGF signaling in common oral diseases is expanding, new potential translational applications emerge. In the present narrative review we aim to explore the role of VEGF signaling in oral cancer and destructive periodontal inflammatory diseases, with emphasis in its translational applications as potential biomarkers and therapeutic targets.

Economic efficiency of road transport vehicles of fleet and its impact on commercial indices and production plan of a motor transport enterprise

At the current stage of development of the branch of motor transport in Ukraine, satisfactory equipment of enterprises with the fixed assets, particularly motor transport enterprises, is one of the main problems. Fleet of road transport vehicles of those enterprises needs almost complete renovation, and productive-technical base for maintenance inspection and repair of transport requires complete technical re-equipment. The aim of the work is to develop theoretical approaches and practical recommendations concerning maintenance of road transport vehicles of motor transport enterprises, choice of the type of transportation means and determination of the necessary number of vehicles depending on the production plan of their operation.

ВЛИЯНИЕ КРИТЕРИЕВ НАДЕЖНОСТИ ПРИ ТЕХНИЧЕСКОМ ОБСЛУЖИВАНИИ МОДУЛЬНЫХ ТРАНСПОРТНЫХ СРЕДСТВ

Criteria for the reliability of maintenance for modular trucks are considered. The diagram of a modular truck is presented. The main criteria of reliability are evaluated. Attention is drawn to reliability as the main parameter of the reliability of a modular vehicle. The value of reliability for technical maintenance and ensuring the maximum technical readiness of modular vehicles is re-vealed. The structure of maintenance and current repair (TR) of a modular car is considered. Con-clusions are made about the importance of reliability in the maintenance of modular trucks.

Стратегии помощи взрослым с РАС. Построение романтических, интимных отношений и семейной жизни

This paper further examines unique aspects of emotional life of adults with ASD and challenges and rewards of building long-term romantic and family relationships. Psychotherapeutic methods of choice, DIRFloortime and mentalization, are aimed at forming, maintenance and flexible repair of the interpersonal relationship: DIRFloortime offers play- and affect-based strategies and helps to build interpersonal relationship, while mentalization techniques provide metacognitive intervention and serve as scaffolding for emotional thinking and higher-level cognition. Techniques of Floortime play, Socratic questions and collateral work proved to be effective. Clinical vignettes illustrate moving the entire family up towards more effective and harmonious co-regulated interaction with corrections to sensory triggers, elements of psychoeducation about self-regulation, and direct self-regulation individual coaching. Coregulating affect, raising self-awareness, demonstrating relationship building and repair, as well as interest in the other in the course of playful interaction; waiting and gently prodding into the emotionally meaningful subjects helps the therapist to help adult autists to erect the societal support system.

Аналитические методы информационного моделирования процессов поддержки принятия решений в системе эксплуатационного контроля Петербургского метрополитена

The author offers the application of analytical methods of information modeling to ensure the effectiveness of complex interrelated processes of maintenance and current repair in the system of operational control of the St. Petersburg Metro. The Data Envelope Analysis (DEA) method and application models developed on its basis can be successfully used for analyzing information situations and making control decisions in systems that require objective analytical processing of «large data bases». One of the most important advantages of using the analytical methods of information modeling in the field of operational control is a significant reduction of the risks of emergency situations due to subjective factors associated with the influence of the final beneficiary of the activity, namely, the citizen.

Comparison of six gas turbine power cycle, a key to improve power plants

It is required to sake methods to improve the power plant performance. Most of the proposed methods can be commenced only in the design stapes. However, the main question of this study is “What can we do to improve the performance of a running power plant?” The first answer to this question is that monitoring the site and periodic overhaul can keep a power plant in its acceptable condition. However, this answer is very qualitatively and needs more precise information like which parameters shall be monitored or which equipment needs more care in the overhaul. In this study, important parameters and the method of their calculations are introduced that must be monitored and compared. Six similar gas turbine power cycles were selected to be compared deeply during a day in this study. In this way, many data were collected every five minutes. Unlike most of the previous studies, this one concerns with maintenance policy and repair strategy. Results of this comparison lead to answer to these questions that which equipment needs special care? Finally, it was shown that in each unit, which equipment needs more attention and which one can be considered as a standard for the others.

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In the military units of the National Guard of Ukraine, a system for repairing automotive vehicles has been introduced, which provides for current, secondary and overhaul repairs. Maintenance of machines is carried out in order to eliminate malfunctions arising from the use of machines, and allows the replacement of individual units, including the main one. The average repair consists in the replacement or overhaul of not more than half of the main units. Overhaul restores the life of the machine and should be carried out at specialized enterprises of the Ministry of Defense of Ukraine or at other enterprises under contracts. Actually, in the military units of NGU, only current repairs are carried out. This is due to the high cost of overhaul and its low quality. Therefore, for the military units of the NGU, a diagnostic and recovery system for repairing automotive vehicles is proposed, including two types of repair: maintenance and restoration. The essence of maintenance repair consists in eliminating malfunctions, violations of adjustments during the operation of the machines according to the inspection, listening ? diagnostics with built-in and external diagnostic devices. Reconditioning repair consists in carrying out repair operations related to disassembling, troubleshooting, mechanical or other processing of component parts, assembling units, assemblies, testing them, and, if necessary, replacing them with new or repaired ones. Maintenance and repair repairs of automotive equipment are carried out only if there is a need for their implementation. It is proposed to establish ten parameters of the diagnostic and recovery system for the repair of automotive equipment at NGU and determine the life of the machine only before being discarding. The procedure for determining the number of machines that must be written off and get new ones to increase the non-reduced resource in each operation group is given.

Методи розподілу витрат на утримання і експлуатацію обладнання при визначенні собівартості промислової продукції

The method of costs allocation for equipment maintenance and operation has been analyzed. It is especially important to properly allocate costs by type of product at those industrial enterprises, where a complex range of products is produced as a result of a single production process. The main method for calculating the product cost unit in industry is calculating the product cost and expense budget. Differentiated methods should be used in allocating indirect costs between particular products or types of products, depending on the nature of a cost, its economic nature, and its connection with the process of forming production cost. In calculating production cost, costs for production maintenance and management are identified, having a very diverse composition. A big group of expenses connected with equipment operation (depreciation on operating equipment and vehicles; maintenance repair of the equipment and shop-floor travelers; wear of low-value tools; the wages of workers engaged in equipment maintenance; maintenance materials) is singled out in a special spending pattern. It is established that quite accurate results are also used in a number of industries. For example, the method of allocation of indirect costs allows the maintenance costs and equipment operation costs to be reasonably allocated, thus making it possible to more accurately calculate the production cost and make management decisions on this basis. It should be noted that, since indirect costs include a large number of various production costs, which differ in many economic respects, allocation of all indirect costs should not be carried out on a single basis.

Cell Sources and Influencing Factors of Liver Regeneration: A Review.

Liver regeneration (LR) is a set of complicated mechanisms between cells and molecules in which the processes of initiation, maintenance, and termination of liver repair are regulated. Although LR has been studied extensively, there are still numerous challenges in gaining its full understanding. Cells for LR have a wide range of sources and the feature of plasticity, and regeneration patterns are not the same under different conditions. Many patients undergoing partial hepatectomy develop cirrhosis or steatosis. The changes of LR in these cases are not clear. Many types of cells participate in LR. Hepatocytes, biliary epithelial cells, hepatic progenitor cells, and human liver stem cells can serve as the cell sources for LR. However, different types and degrees of damage trigger the response from the most suitable cells. Exploring the cell sources of LR is of great significance for accelerating recovery of liver function under different pathological patterns and developing a cell therapy strategy to cope with the shortage of donors for liver transplantation. In clinical practice, the background of the liver influences regeneration. Fibrosis and steatosis create different LR microenvironments and signal molecule interaction patterns. In addition, factors such as partial hepatectomy, aging, platelets, nerves, hormones, bile acids, and gut microbiota are widely involved in this process. Understanding the influencing factors of LR has practical value for individualized treatment of patients with liver diseases. In this review, we have summarized recent studies and proposed our views. We discuss cell sources and the influential factors on LR to help in solving clinical problems.

Research of the effect of temporary reservation on the reliability of equipment locomotives

The article deals with the issues of the effect of the temporary reservation of the work of a separate locomotive equipment on its reliability, under the conditions different laws of distribution of time of work, time of recovery, time for temporary reservation. At the same time, the issue of the influence of the random nature of the occurrence of refusals with the justification of the strategy of their prevention is considered. The effect of temporary reservation of work of separate equipment of locomotives on its reliability is investigated. Calculation takes place taking into account different laws of distribution of time of work to the failure, time on recovery, time on temporary reservation. This research is relevant because, in recent years, in our country gradually develops high-speed railway traffic. In the majority, the high-speed rolling stock (SRS) operating in the country, built abroad, and not always the mode of its operational loads meets the recommendations of suppliers of this technique. This leads to a breakdown in train traffic and great downtime, due to both the general condition of the railways and the intensity of the use of this SRS. One more negative factor is the problem of organization of technical maintenance (TM) and current repair (СR) of this rolling stock. But not only for SRS there are such problems, because the current traction rolling stock has reached or exceeded the normative term of use, the repair base is updated slowly, technological processes TM and СR are not corrected during the life cycle. All this requires both practical and theoretical substantiation of the strategy and tactics of TM and СR of the transport rolling stock. Therefore, there were formulated and scientifically substantiated methods for ensuring and improving the reliability of technique, on the basis of the analyzed functional relationships between the reliability indicators and the calculated ratios for reliability indicators of complex systems, which are is the traction rolling stock and locomotives. It has been determined that the temporary reservation of the work of the locomotive systems significantly affects the time to the failure, recovery time and readiness factor and significantly improves these parameters, in contrast to the system performance without temporary reservation. It is also concluded that it is advisable to organize the recovery process in such a way that the distributions of time for recovery were close to gamma-distribution. And the value of the readiness factor depends significantly on the type of the law of distribution of exploitation to failure and the type of the law of distribution of time for recovery.

ВИЗНАЧЕННЯ НОРМ ЧАСУ НА ТЕХНІЧНЕ ОБСЛУГОВУВАННЯ ТРАНСПОРТНОГО ЗАСОБУ ДЛЯ ФОРМУВАННЯ ТРУДОМІСТКОСТІ РОБІТ

У роботі розглянуті актуальні питання визначення норм часу на обслуговування і ремонт транспортних засобів для формування трудомісткості робіт технічного обслуговування та поточного ремонту рухомого складу підприємств автомобільного транспорту, що використовуватимуться під час їх технологічного розрахунку. На основі існуючих норм часу на виконання різних видів робіт запропоновано граф технічного обслуговування (ремонту) автомобіля за яким розроблено методику визначення норм часу та трудомісткості технічних впливів на автомобіль. Граф технічного обслуговування (ремонту) транспортних засобів служить графічною моделлю для подальших розрахунків норм часу та забезпечує перехід від об’єкта впливу до елементарних операцій. Такий підхід дозволяє розподілити елементарні операції і врахувати особливості їх виконання. Розроблена методика сприяє подальшому розвитку методів визначення трудомісткості технічного обслуговування для технологічного розрахунку підприємств автомобільного транспорту. Проведено аналіз величин трудомісткостей технічного обслуговування транспортних засобів. На прикладі, проведення регламентних робіт з технічного обслуговування нижчого порядку для автобуса за допомогою методів аналізу затрат робочого часу та використовуючи норми часу на слюсарні операції, здійснено орієнтовний розрахунок його сумарної трудомісткості. Отримані результати вказують на скорочення часу виконання операцій технічного обслуговування автобуса, а від так і зменшення встановленої трудомісткості близько 40 % у порівнянні з нормативом.

An Example of Primary School Buildings in Akçakoca, Belongs to Early Republican Period: Orhan Gazi Primary School

Cumhuriyet’in ilanıyla birlikte birçok alanda köklü değişimler yaşanmaya başlamıştır. Eğitim ve mimarlık değişimlerin hızlı yaşandığı alanların başında gelmektedir. Eğitim, halkın bilinçlenmesi açısından önemli bir role sahipken; mimarlık ise modernitenin mekânsal temsili açısından kritik rol oynamaktadır. Dolayısıyla, Erken Cumhuriyet döneminde eğitim yapılarının birçok açıdan önemli misyonlara sahip olduğunu söylemek mümkündür. Temel eğitimin verildiği ilkokullar ise; diğer kademelerdeki eğitim kurumlarından daha öncelikli olarak değerlendirilmiştir. Halkın çoğunluğunun henüz okuma-yazma bilmiyor olması nedeniyle; hızlı bir şekilde okur-yazar oranının arttırılması amaçlanmıştır. Bununla birlikte; Cumhuriyet ilkelerine bağlı ilköğretim okullarının yaygınlaştırılarak herkesin ilkokul eğitimi alması hedeflenmiştir. Bu kapsamda; kentlerde, kasabalarda ve köylerde yeni okul binaları tasarlanarak inşa edilmiştir. Cumhuriyetin ilk yıllarında inşa edilen eğitim yapıları; I. Ulusal Mimarlık Dönemi olarak adlandırılan mimari üslubu yansıtmaktadır. Çalışmaya konu olan Orhan Gazi İlkokulu da bahsedilen misyonla Akçakoca’da 1. Ulusal Mimarlık Üslubunun özellikleriyle inşa edilmiştir. Çalışmayla amaçlanan; dönemin mimari özellikleri paralelinde incelenen yapının Düzce ve Akçakoca’nın Erken Cumhuriyet dönemi mimarlık üretimine ışık tutması ve daha sonra yapılacak çalışmalara örnek teşkil etmesidir.

Type I IFNs: A Blessing in Disguise or Partner in Crime in MERS-CoV-, SARS-CoV-, and SARS-CoV-2-Induced Pathology and Potential Use of Type I IFNs in Synergism with IFN-γ as a Novel Antiviral Approach Against COVID-19.

Since the end of 2019, the emergence of novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has accelerated the research on host immune responses toward the coronaviruses. When there is no approved drug or vaccine to use against these culprits, host immunity is the major strategy to fight such infections. Type I interferons are an integral part of the host innate immune system and define one of the first lines of innate immune defense against viral infections. The in vitro antiviral role of type I IFNs against Middle East respiratory syndrome coronavirus (MERS-CoV) and SARS-CoV (severe acute respiratory syndrome coronavirus) is well established. Moreover, the involvement of type I IFNs in disease pathology has also been reported. In this study, we have reviewed the protective and the immunopathogenic role of type I IFNs in the pathogenesis of MERS-CoV, SARS-CoV, and SARS-CoV-2. This review will also enlighten the potential implications of type I IFNs for the treatment of COVID-19 when used in combination with IFN-γ.

Evaluation of the influence of the mode of movement of “Volgabus” buses on the performance of the clutch

The article gives evaluation of the clutch performance of “Volgabus-5270GH” MUE “Volzhskaya №1732 bus depot” based on bus removals with different mileage values. Scheduled maintenance and repair system implies the elimination of bus removals and repair works between MO1 (MO - Maintenance Operations) and MO2. Authors have carried out the analysis of clutch malfunctions. Based on many studies, it has been established that the service life of parts and components of vehicles in kilometers of run, under proper operation, obeys the law of normal distribution. It is defined that untimely bus removals with repair under condition of qualitative manufacturing and timely scheduled maintenance repair (SMR) are also possible under influence of operational factors of a route network of Volzhsky. The analysis of bus movement modes on the main routes and their possible connection with clutch malfunctions has been carried out. The article gives recommendations for increasing the efficiency of bus clutch.

Внедрение и адаптация методов математического моделирования нелинейных процессов на примере эксплуатации транспортных средств и специальной техники

The modern mathematical modeling methods provides a comprehensive toolkit for handling a most of data, on another hand more complicated tasks requires logic with higher computational complexity, which means a considerable influence at a Big Data handling. The most suitable method selection is a search of a compromise solution with the necessary accuracy and admissible computational complexity. Moreover, methods are to be adapted to the certain solution aimed at providing the greatest match of the modeling results to process class. This article discusses the selecting and adaptation of mathematical modeling methods to vehicles and heavy equipment MRO costs, which are a result of two different processes: regular maintenance and accidental repair that may be delayed due to the situation reasons. The work done not only allows to make a longterm (thirty years) forecast using accumulated during a short time (three years) statistics, but also provides a toolkit to handle a wide range of data including non-linear and stochastic processes.

Air void and ring gap effect on CFST arch bridges dynamic performance

Many old concrete-filled steel tube (CFST) arch bridges constructed using common concrete, the fluidity before initial setting and dry shrinkage during use create ring gaps at the interfaces of concrete core column (CCC) and spiral welding tube (SWT), and air voids in CCC. Also, unfavorable conditions of nature increase the number of gaps and voids, create more serious defects, decrease arch rib stiffness, and even change the dynamic properties of the whole bridge structure. Therefore, a finite element analysis (FEA) verified by scale-model experiments on CFST arch bridge rib members with air void and ring gap composite defects was conducted to determine the tangential stiffness of the structure. Secondly, the natural vibration and frequency of the whole bridge were analyzed using tangential stiffness and the influences of various defects on the dynamic performance of bridge was investigated. Therefore, a novel trans-scale determine system was developed for the detection of the tangential stiffness of bridges with composite defects in CFST ribs, so as to evaluate the influences of these defects on the dynamic performance of bridges. This study can provide a strong foundation for the maintenance repair of CFST arch bridges.

Lysine acetyltransferase Tip60 is required for hematopoietic stem cell maintenance

AbstractHematopoietic stem cells (HSCs) have the potential to replenish the blood system for the lifetime of the organism. Their 2 defining properties, self-renewal and differentiation, are tightly regulated by the epigenetic machineries. Using conditional gene-knockout models, we demonstrated a critical requirement of lysine acetyltransferase 5 (Kat5, also known as Tip60) for murine HSC maintenance in both the embryonic and adult stages, which depends on its acetyltransferase activity. Genome-wide chromatin and transcriptome profiling in murine hematopoietic stem and progenitor cells revealed that Tip60 colocalizes with c-Myc and that Tip60 deletion suppress the expression of Myc target genes, which are associated with critical biological processes for HSC maintenance, cell cycling, and DNA repair. Notably, acetylated H2A.Z (acH2A.Z) was enriched at the Tip60-bound active chromatin, and Tip60 deletion induced a robust reduction in the acH2A.Z/H2A.Z ratio. These results uncover a critical epigenetic regulatory layer for HSC maintenance, at least in part through Tip60-dependent H2A.Z acetylation to activate Myc target genes.

IDENTIFIKASI POLA ALIRAN DI SEKITAR DAERAH GENANGAN BANJIR

Johan Pahlawan Sub-district especially Leuhan Village and Blang Beurandang Village is a residential area that was hit by a flood inundation. Rainfall intensity and flow pattern conditions cause inundation. Puddles result in physical damage to home components and cause a number of losses, with varying levels of inundation and water levels. This analysis study was conducted with the aim of identifying the condition of the pattern of drainage flow around the flood inundation area. The research approach is in the form of quantitative and qualitative research. Identification results obtained: The pattern of land flow and drainage in the form of flow direction is in accordance with the topographic conditions of the study area, with channel conditions such as shrubs, constriction and at certain points with conditions of low contoured areas so that inundation is prone to improvements such as maintenance activities and repair.