Looped Structure Research Articles

CircRNA Regulation of T Cells in Cancer: Unraveling Potential Targets.

T lymphocytes play a critical role in antitumor immunity, but their exhaustion poses a significant challenge for immune evasion by malignant cells. Circular RNAs (circRNAs), characterized by their covalently closed looped structure, have emerged as pivotal regulators within the neoplastic landscape. Recent studies have highlighted their multifaceted roles in cellular processes, including gene expression modulation and protein function regulation, which are often disrupted in cancer. In this review, we systematically explore the intricate interplay between circRNAs and T cell modulation within the tumor microenvironment. By dissecting the regulatory mechanisms through which circRNAs impact T cell exhaustion, we aim to uncover pathways crucial for immune evasion and T cell dysfunction. These insights can inform innovative immunotherapeutic strategies targeting circRNA-mediated molecular pathways. Additionally, we discuss the translational potential of circRNAs as biomarkers for therapeutic response prediction and as intervention targets. Our comprehensive analysis aims to enhance the understanding of immune evasion dynamics in the tumor microenvironment by facilitating the development of precision immunotherapy.

Facile fabrication of non-fluorine polymer brush/loop surfaces for oil/water separation and self-cleaning applications

Polydimethylsiloxane (PDMS), a low surface energy material, is widely used in the preparation of various (super)hydrophobic materials. Nevertheless, the impact of PDMS chain architectures (i.e., linear, branched, difunctional and monofunctional PDMS) on surface properties, such as hydrophobicity and self-cleaning, has received limited attention. In this work, we constructed PDMS chains on stainless steel mesh/polyester fiber/silica surfaces to form both looped and linear topological structures. Surfaces with looped structure showed higher roughness and stronger hydrophobicity in air and water, compared to the surfaces with linear structures. Surface force measurements demonstrated that the silica surface with looped structure exhibited stronger hydrophobic forces in water than the surfaces with linear structure. The looped structure coatings also exhibited superior self-cleaning and faster water sliding compared to the linear structure coatings. Additionally, both looped and linear PDMS coated stainless steel mesh/polyester fibers showed efficient separation of oil/water mixture. This study sheds light on the influence of polymer topology on surface properties and advances the development of applications in oil/water separation and self-cleaning.

A New Approach to Design Triple-Band Filtering Power Dividers Based on Coupled Lines

In this letter, a new approach to designing triple-band filtering power divider (FPD) based on coupled lines is proposed. A resistor loaded looped coupled-line structure is utilized to realize the indispensable in-band isolation and power division. Moreover, eight coupled lines that are used in cascaded or loaded ways are imported to introduce multiple transmission zeros (TZs) and transmission poles (TPs) for stopband rejection and multipassband transmission. Four of these coupled lines are also used for controlling bandwidth. Finally, to demonstrate the authors’ design concept, a prototype operating at 2.3, 3.1, and 3.9 GHz with 3-dB fractional bandwidths (FBWs) of 17.4%, 13.0%, and 10.0% is simulated, fabricated, and measured, respectively. The tested results are consistent with the simulation results of electromagnetic simulation software.

Hsa_circ_0000851 promotes PDK1/p-AKT-mediated cell proliferation and migration by regulating miR-1183 in triple-negative breast cancer

Breast cancer (BC) is the most common cause of cancer-related mortality in women worldwide. Circular RNAs (circRNAs), a type of non-coding RNA, have garnered interest because of their unique looped structure. In recent years, circRNAs have been shown to be involved in various diseases, including carcinogenesis, and to serve as biomarkers for early risk assessment and survival prediction of different tumour types. This study aimed to identify a novel circRNA, hsa_circ_0000851, generated from the sixth intron of the oncogene TCF4, reported to be involved in BC pathogenesis. Our study showed that hsa_circ_0000851 was mainly located in the cytoplasm of BC cells and upregulated in BC cell lines and tissue samples. Higher hsa_circ_0000851 expression levels resulted in increased proliferation of BC cells both in vitro and in vivo, while treatment of BC cells with hsa_circ_0000851 siRNA decreased their proliferation. We found that hsa_circ_0000851 bound directly to miR-1183, accelerating the expression of its target gene PDK1, which facilities BC cell proliferation and migration through PDK1/p-AKT.

Identification of model flow parameters and model coefficients with the help of integrated measurements of pipeline system operation parameters

The problem of state and parameter estimation of natural gas pipeline system under stationary and non-stationary gas flow is considered. The initial information for solving the problem are pressure and flow measurements with standard measuring equipment. Measurement errors are considered random values that have a normal distribution with zero expectation. The developed methodology takes into account the whole complex of measured parameters in their relationship. The problem reduces to an optimization task; the objective function is derived from the maximum likelihood method. The effectiveness of the methodology was tested on a real object – a complex gas distribution system of a looped structure. The hydraulic efficiency coefficients for this facility are estimated in stationary and non-stationary modes. We use a non-standard model with lumped parameters, which allows us to switch from a system of partial differential equations to a system of ordinary differential equations connecting nodal pressures and flow rates at the arcs of the network graph. In a computational experiment using the gas pipeline branch as an example, the developed algorithm showed very fast convergence.

Truncated Microstrip Patch Antenna with DGS Based on Double Looped CSRR Arrays for Enhancement of Bandwidth

This paper presents a novel method for the design of truncated microstrip patch antenna with DGS based on Double Looped CSRR arrays for enhancement of bandwidth. The proposed antenna is suitable for WLAN/Wi-Fi, RFID applications. A truncated microstrip patch antenna without DGS was initially designed for a resonant frequency of 2.86GHz having a bandwidth of 95.6MHz. By integrating a new Double Looped CSRR array (DLCSRR) structure on the ground plane, resonant frequency of the antenna is shifted to 2.47GHz and also the bandwidth is enhanced to 202.5MHz. By implementing DLCSRR array structure on the ground plane size reduction and bandwidth enhancement of 52.79% is achieved. The performance of truncated microstrip patch antenna with DGS based on Double Looped CSRR array structure was also compared by replacing conventional proximity fed microstrip patch antenna with the same physical size. The conventional proximity coupled microstrip patch antenna is designed for a resonant frequency of 2.26GHz with a lower bandwidth of 23.3MHz.The antennas were designed using CST simulation software. The measurement result shows a good agreement with simulated results.

Truncated Microstrip Patch Antenna with DGS Based on Double Looped CSRR Arrays for Enhancement of Bandwidth

This paper presents a novel method for the design of truncated microstrip patch antenna with DGS based on Double Looped CSRR arrays for enhancement of bandwidth. The proposed antenna is suitable for WLAN/Wi-Fi, RFID applications. A truncated microstrip patch antenna without DGS was initially designed for a resonant frequency of 2.86GHz having a bandwidth of 95.6MHz. By integrating a new Double Looped CSRR array (DLCSRR) structure on the ground plane, resonant frequency of the antenna is shifted to 2.47GHz and also the bandwidth is enhanced to 202.5MHz. By implementing DLCSRR array structure on the ground plane size reduction and bandwidth enhancement of 52.79% is achieved. The performance of truncated microstrip patch antenna with DGS based on Double Looped CSRR array structure was also compared by replacing conventional proximity fed microstrip patch antenna with the same physical size. The conventional proximity coupled microstrip patch antenna is designed for a resonant frequency of 2.26GHz with a lower bandwidth of 23.3MHz.The antennas were designed using CST simulation software. The measurement result shows a good agreement with simulated results.

Gold Complexes with Difunctional Perfluoroalkyl Chains: Quantifying the Energy of Aurophilic Interactions in Flexible Open‐Chain Complexes

AbstractThe first binuclear AuI compounds containing bridging (CF2)n chains (n=4, 6, 8) and AuIII metallaperfluorocyclopentanes have been obtained by photoinitiated reactions of LAuMe (L=PPh3, PMe3, PCy3, or IPr) with α,ω‐diiodoperfluorocarbons. Complexes LAu(CF2)4AuL present an unusual looped structure stabilized by an aurophilic interaction for L=PMe3, PPh3, and PCy3. The study of their dynamic behaviour has provided new insights about the strength of aurophilic interactions in solution, allowing quantification of the energy of a single Au⋅⋅⋅Au interaction.

Gold Complexes with Difunctional Perfluoroalkyl Chains: Quantifying the Energy of Aurophilic Interactions in Flexible Open-Chain Complexes.

The first binuclear AuI compounds containing bridging (CF2 )n chains (n=4, 6, 8) and AuIII metallaperfluorocyclopentanes have been obtained by photoinitiated reactions of LAuMe (L=PPh3 , PMe3 , PCy3 , or IPr) with α,ω-diiodoperfluorocarbons. Complexes LAu(CF2 )4 AuL present an unusual looped structure stabilized by an aurophilic interaction for L=PMe3 , PPh3 , and PCy3 . The study of their dynamic behaviour has provided new insights about the strength of aurophilic interactions in solution, allowing quantification of the energy of a single Au⋅⋅⋅Au interaction.

HAVA-JETLİ TEKSTÜRE PBT İPLİKLERİN MEKANİK ÖZELLİKLERİNİN VE KARARSIZLIK DAVRANIŞLARININ İNCELENMESİ

Hava-jetli tekstüre işlemi, sentetik filamentlerin iplik içerisindeki düzenli yerleşimlerini mekanik yolla değiştirerek, bu filamentleri birbirine karışmış, hacimli, doğal ipliklere benzer bir yapıya dönüştürür. Bu teknikte, uygulanan aşırı beslemenin yardımıyla her çeşit filament, tekstüre jetinden uygulanan süpersonik hava akımının tesiriyle karıştırılabilir. Hava-jetli tekstüre işleminde jetten tekstüre olarak çıkan ipliğe, alım silindiri ile sevk silindiri arasında bir mekanik germe işlemi uygulanır. Mekanik germenin amacı, tam olarak ilmek oluşturamamış filamentlerin çözülmesi ve ilmek oluşturabilmiş filamentlerin daha iyi kilitlenerek ipliğin mekanik özelliklerini daha kararlı hale getirmektir. Hava-jetli tekstüre ipliklerin yapısında bulunan ilmeklerin bir kısmı uygulanan kuvvetlerin etkisiyle yok olabilir, iplik üzerinde kalan ilmeklerin miktarı bu ilmeklerin “kararlılığı” ile ilişkilidir. Poliesterler ana zincirlerinde ester bağları bulunduran sentetik liflerdir. Bu lifler olumlu performans özellikleri nedeniyle konvansiyonel ve endüstriyel alanlarda sıklıkla tercih edilmektedir. Farklı poliester çeşitleri arasında, polibütilen tereftalat (PBT) lifleri özellikle iyi toparlanma davranışının istendiği uygulamalarda tercih edilmektedir. Bu çalışmada hava-jetli tekstüre PBT ipliklerin mekanik özellikleri ve kararsızlık davranışları incelenmiştir. Yapılan ön denemelerde, mekanik germe oranının %6’nın üzerinde olduğu durumlarda iplik yapısında deformasyon gözlenmiştir. Bu nedenle bu çalışmada en yüksek %6 olacak şekilde 4 farklı mekanik germe (%0-2-4-6) ve 3 farklı aşırı besleme oranında (%20-40-60) hava-jetli tekstüre PBT iplikler üretilmiştir. Üretilen bu numunelerin, iplik numaraları, mekanik özellikleri ve elastik toparlanma davranışları incelenmiştir. Çekme ve elastik toparlanma deneylerinden elde edilen sonuçlar optik mikroskop deneyleriyle ilişkilendirilerek açıklanmıştır. Yapılan deneyler sonucunda, hava-jetli tekstüre PBT ipliklerde aşırı besleme oranındaki artışa bağlı olarak ilmekli yapıda bir artış gözlenmiştir. Bu durum, numunelerin iplik numarası ve kopma uzaması değerlerinde bir artışa, elastik toparlanma davranışlarında ise azalmaya neden olmuştur. En az ilmekli yapının gözlendiği %20 aşırı besleme ile üretilen numunelerde filamentler iplik eksenine olan paralelliklerini kısmen korumuştur. Dolayısıyla bu numuneler için hava-jetli tekstüre iplik yapısından ziyade filamentleri meydana getiren molekül zincirlerinin özellikleri ön plana çıkmış ve en iyi toparlanma bu iplik grubunda gözlenmiştir.

The design and development of an illuminated polymeric optical fibre (POF) knitted garment

This paper presents a new design and development process for integrating illuminated Polymeric Optical Fibre (POF) into knitted garments, by incorporating POF directly into the looped structure. Fully-fashioned knitting is presented as a means of overcoming garment design restrictions in existing works. A key challenge this work addresses is the creation of more appealing and fashionable form-fitting illuminated POF garments. The resultant work, a tight-fitting knitted raglan sleeve jumper with a section of knitted POF demonstrates that new aesthetics can be achieved, with our new technique. This project builds on existing research into POF integration into fashion and textiles, whilst challenging the existing view that POF cannot be formed into a looped structure. This project used a hand flat knitting machine to produce the knitted POF garment, to balance the benefits of using handcraft techniques for challenging fibres, with the faster knitting speed better associated with using knitting machines.

Abstract 200: Targeting the Highly Abundant Circular RNA, circHeart, in Cardiomyocytes Attenuates Pressure Overload Induced Hypertrophy

Objectives: Circular RNAs (circRNAs) are single-stranded non-coding RNAs that form a covalent closed looped structure and are generated during gene transcription. In our genome-wide analysis of cardiac circRNA expression in both human and mouse hearts we identified a highly abundant circRNA, named here as circHeart and hypothesize an important role for circHeart in cardiomyocyte physiology and homeostasis. Materials and method: We undertook FISH to visualize the localization of circHeart . Online tools have predicted multiple potential miRNA binding sites on circHeart , including the cardiac-enriched microRNA-133a (miR-133a). We assessed the interactions of circHeart with these miRNAs by luciferase and pull-down assays and performed AAV9-mediated RNAi knockdown and overexpression of circHeart in mice in vivo . In vivo phenotype was tracked by echocardiography, electrocardiogram and histological studies. Transverse aortic constriction (TAC) was also performed. Results: FISH imaging showed circHeart localized largely to the cardiomyocyte cytosol. Luciferase and reciprocal pull-down assays suggest circHeart can interact with miR-133a. MiR-133a is a well-known regulator of cardiac hypertrophy and antagomir-133a treatment has been shown to induce cardiac hypertrophy in vivo . In our studies, while AAV9 RNAi-mediated knockdown of circHeart has no apparent baseline effect in the heart it suppresses pathological cardiac hypertrophy post-TAC. More strikingly, AAV9-mediated overexpression of circHeart in vivo resulted in mouse mortality between 3-4 days post-transduction and surviving mice showed evidence of dilated cardiomyopathy. Known targets of miR-133a were concordantly modulated in the knockdown and overexpression mouse model. Conclusion: CircRNAs have huge potential role as therapeutic targets and/or disease biomarkers and represent a new avenue for cardiovascular research. Taken together, our data are consistent with a mechanistic model of circHeart sequestering miR-133a. Inhibiting circHeart abundance presents a potential target for treating cardiac hypertrophy.

Electrochemiluminescence sensing platform for ultrasensitive DNA analysis based on resonance energy transfer between graphitic carbon nitride quantum dots and gold nanoparticles

Electrogenerated chemiluminescence (ECL) of semiconductor quantum dots (QDs) is considered as a powerful technique in the fabrication of biosensor, however, the inherent toxicity of the heavy metal ion containing in QDs limits their further applications. Thus, searching for environment-friendly luminescent nanomaterials with high electrochemiluminescence (ECL) efficiency is an urgent goal. In this work, a solid-state method under low temperature was adopted to prepare graphitic carbon nitride quantum dots (g-CNQDs). By using coreactant K2S2O8, a strong cathodic ECL signal of g-CNQDs could be observed in phosphate buffer. A novel ECL resonance energy transfer procedure was constructed between g-CNQDs (emitter) and gold nanoparticles (acceptor). A signal probe was formed by connecting gold nanoparticles at the hairpin DNA (Hai-DNA) terminal. When the signal probe was anchored on g-CNQDs, ECL resonance energy transfer occurred due to the ECL quenching of gold nanoparticles to g-CNQDs. This phenomenon decreased the ECL signal. In the presence of target DNA (T-DNA), the looped structure of Hai-DNA could be destroyed by T-DNA, and gold nanoparticles were separated from g-CNQDs. Accordingly, the ECL resonance energy transfer procedure was hindered, and the ECL signal was recovered again. The ECL intensities exhibited linear correlation with the logarithm of T-DNA concentration from 0.02 fM to 0.1 pM, and the limit of detection was 0.01 fM (3σ). With the developed ECL resonance energy transfer system, good selectivity and high sensitivity were achieved in T-DNA detection.

Low-Cost and High-Efficiency Solar-Driven Vapor Generation Using a 3D Dyed Cotton Towel.

Solar‐driven vapor generation is a promising method to mitigate freshwater shortage and water contamination. However, most of the current highly efficient solar evaporators suffer from low robustness, tedious preparation procedures, and high cost. In this study, an easy‐to‐manufacture, low‐cost, and high‐reliability solar‐driven evaporator is designed using a black cotton towel with a hollow conical shape. The reactive dye molecules diffuse into the cotton and form strong covalent bonds with the fiber after dyeing, which firmly fixes light‐absorbing materials on the substrate. The looped pile structure of towels and hierarchical structure of yarns enable the evaporator enlarged surface area. The hollow conical shape of the cotton towel can effectively suppress the heat loss to the environment without compromising light absorption. The 3D vapor generator exhibits an evaporation rate of 1.40 and 1.27 kg m−2 h−1 for pure water and saline water, respectively. Meanwhile, this towel‐based solar‐driven evaporator exhibits a promising antifouling property as well as superior reusability and provides a reliable pathway in dealing with realistic waters, such as seawater and dyeing sewage. Therefore, the low‐cost, solar‐driven water evaporation system offers a complementary approach for high‐efficiency vapor generation and water purification in practical application.

Report of a non-looped variant of ansa cervicalis with omohyoid innervation from accessory nerve branch and omohyoid attachment to mastoid process.

A variant of the innervation of the infrahyoid neck musculature is reported in which the typical looped ansa cervicalis structure is absent. In this variant, the infrahyoid muscles (sternohyoid, sternothyroid omohyoid and thyrohyoid) were innervated by a presumptive superior root of "ansa cervicalis" traveling with vagus nerve (CN X) and not branching from hypoglossal nerve (CN XII). The omohyoid muscle, typically innervated by the inferior root of ansa cervicalis, is instead innervated by nerve fibers branching from the accessory nerve (CN XI). This formation created a non-looping variant of ansa cervicalis. Furthermore, the omohyoid muscle did not attach to the hyoid bone but instead attached to the mastoid process of the temporal bone by merging its fibers superiorly and posteriorly with the clavicular portion of the sternocleidomastoid muscle, creating a "sternocleidoomomastoid" muscle innervated by a branch of accessory nerve. This variation was found in one black male cadaver from a cohort of 25 male and female cadavers. Only one variation of ansa cervicalis was observed. As neck dissections and surgical procedures of this region are performed for a variety of conditions-including coronary artery bypass grafting and metastatic neck disease-variations of this type are of broad clinical surgical importance.

Preliminary report of a new type of braided vein stent in animals.

Iliofemoral vein stenosis or occlusion is a common cause of severe chronic venous insufficiency. Endovascular venous stenting has become a preferred treatment because it is minimally invasive and has a high safety profile. Despite the wide application of the wallstent, it is not specifically designed for veins. There are currently few studies on braided stents in the field of veins. We designed a novel braided vein stent, which has higher radial resistive force and more optimized looped ends structure compared with the wallstent. The purpose of this study was to evaluate the safety and performance of the stent in animals, providing a reference for further clinical trials. The Wallstent is used as a control group. The novel stent and the Wallstent were implanted in the iliac vein of sheep. After 30 days and 90 days, vascular injury, thrombus, neointima coverage, and luminal stenosis were evaluated through venous angiography, endoscopic observation of stent specimen and histopathology. Imaging, histology, and integration data were analyzed by t-test for comparisons between the groups. Two groups of stents were successfully implanted. Follow-up observation showed that there was no thrombosis or obstruction >50% occurred in any group and no significant differences in patency, vascular injury, or intimal hyperplasia compared with the Wallstent. The novel stent significantly increases the radial resistive force and does not increase vascular injury, thrombus and stent stenosis during 30-day and 90-day follow-up. The next step is to further validate the effectiveness of the stent through long-term animal observation and human clinical trials.

Interaction between Sso7d and DNA studied by single-molecule technique

Each organism has its own set of chromatin proteins to protect the stable structure of DNA and thus maintain the stability of genes. Sso7d is a small nonspecific DNA-binding protein from the hyperthermophilic archaea Sulfolobus solfataricus. This protein has high thermal and acid stability. It stabilizes dsDNA and constrains negative DNA supercoils. Besides, the Sso7d binds in a minor groove of DNA and causes a sharp kink in DNA. By observing the interaction between chromatin protein and DNA structure, we can understand the function and mechanism of chromatin protein. Sulfolobus solfataricus can survive at high temperature. To understand why the DNA of Sulfolobus solfataricus retains activity at high temperature, we investigate the interaction between Sso7d and DNA by atomic force microscope (AFM) and magnetic tweezers. Atomic force microscope and magnetic tweezers are advanced single molecule experimental tools that can be used to observe the interaction between individual molecules. The experimental result of AFM reveals the process of interaction between Sso7d and DNA. The DNA structure changes at a different concentration of Sso7d and depends on reaction time. At a relatively low concentration of Sso7d, DNA strand forms a kink structure. When the concentration of Sso7d is increased, DNA loops appear. Finally, DNA becomes a dense nuclear structure at a high concentration of Sso7d. If the time of the interaction between Sso7d and DNA is increased, DNA structure tends to be more compact. These results indicate that high concentration of Sso7d is important for the compact structure of DNA. We design an experiment to find out the formation of the looped structure on DNA. Moreover, we measure the angle of kinked DNA and compared it with previous result. Through the experiment of magnetic tweezers, we measure the forces of unfolding the double-stranded DNA complexed with Sso7d at different concentrations. The experimental results show that the binding between Sso7d and DNA increases the force of unfolding the double-stranded DNA. The binding energy between Sso7d and dsDNA is 3.1kBT which is calculated from experimental data. It indicates that DNA base pairs are more stable when chromatin protein Sso7d exists. These results can explain the survival of Sulfolobus in high temperature environment.

Crystal structure of fipronil.

The title compound, C12H4Cl2F6N4OS {systematic name: 5-amino-1-[2,6-di-chloro-4-(tri-fluoro-meth-yl)phen-yl]-4-[(tri-fluoro-methane)sulfinyl]-1H-pyrazole-3-carbo-nitrile}, is a member of the phenyl-pyrazole group of acaricides, and one of the phenyl-pyrazole group of insecticides. The dihedral angle between the planes of the pyrazole and benzene rings is 89.03 (9)°. The fluorine atoms of the tri-fluoro-methyl substituent on the benzene ring are disordered over two sets of sites, with occupancy ratios 0.620 (15):0.380 (15). In the crystal, C-N⋯π inter-actions [N⋯ring centroid = 3.607 (4) Å] together with N-H⋯N and C-H⋯F hydrogen bonds form a looped chain structure along [10[Formula: see text]]. Finally, N-H⋯O hydrogen bonds and C-Cl⋯π inter-actions [Cl⋯ring centroid = 3.5159 (16) Å] generate a three-dimensional structure. Additionally, there are a short inter-molecular F⋯ F contacts present.

A numerical study of a looped-tube thermoacoustic engine with a single-stage for utilization of low-grade heat

Multi-stage thermoacoustic engines with a looped structure have become a promising technology to use heat at a low temperature level. This study forwards a single-stage engine with comparable capabilities. A stub is used to suppress acoustic impedance disturbances. This conception shows to be a powerful tool to accurately tune acoustic conditions in the regenerator. Furthermore, a systematic parametric study is carried out numerically with DeltaEC to examine their relations. This could help in a system-wide optimization according to various application requirements. The study comprises geometrical parameters of regenerator, heat exchangers, and feedback loop as well as process parameters like operational temperatures, mean pressure and working gas. Discrepancies between ideal conditions on component level and on system level have been observed and an approach to interpret them is given. Recommendations for parameter settings depending on an efficiency or power driven operation are supplied. Since a proper coupling between acoustic load and acoustic field in the loop is essential for a sufficient overall-performance, joining mechanisms have been examined in terms of acoustic impedance and position of the load. Eventually it is shown that the proposed engine type is able to power an acoustic load with a relative Carnot efficiency greater than 50% when heat is supplied at 150°C and rejected at 15°C.

A new hydraulic regulation method on district heating system with distributed variable-speed pumps

Compared with the hydraulic configuration based on the conventional central circulating pump, a district heating system with distributed variable-speed-pumps configuration can often save 30–50% power consumption on circulating pumps with frequency inverters. However, the hydraulic regulations on distributed variable-speed-pumps configuration could be more complicated than ever while all distributed pumps need to be adjusted to their designated flow rates. Especially in a multi-source looped structure heating network where the distributed pumps have strongly coupled and severe non-linear hydraulic connections with each other, it would be rather difficult to maintain the hydraulic balance during the regulations. In this paper, with the help of the advanced automation and information technologies, a new hydraulic regulation method was proposed to achieve on-site hydraulic balance for the district heating systems with distributed variable-speed-pumps configuration. The proposed method was comprised of a new hydraulic model, which was developed to adapt the distributed variable-speed-pumps configuration, and a calibration model with genetic algorithm. By carrying out the proposed method step by step, the flow rates of all distributed pumps can be progressively adjusted to their designated values. A hypothetic district heating system with 2 heat sources and 10 substations was taken as a case study to illustrate the feasibility of the proposed method. Two scenarios were investigated respectively. In Scenario I, the flow rate of one substation varied according to its heat demand and the flow rates of other substations maintained their original values. And in Scenario II, the flow rates of all substations varied synchronously with the same relative rate. The results of the both scenarios indicated that all pumps could be properly adjusted to their designated flow rates by the proposed method with a high frequency adjustment resolution as 0.001Hz. In scenario I, compared with the district heating system with distributed variable-speed-pumps configuration, the power consumption would be 26.6–66.8% less than that of the conventional central circulating pump configuration during the 4 rounds of regulations. In scenario II, the energy saving ratio of the district system with distributed variable-speed-pumps configuration would be 36.1–90.3% less than that of the conventional central circulating pump configuration during the 5 rounds of regulations.