Local Temperature Control Research Articles

Controlling Temperature and Chemical Environment for Patch Clamp Studies of TRP Channels

A microfluidic device fabricated in PDMS, which permits control of local temperature and rapid exchange of solution around a single cell, has recently been developed. The system allows an experimental design where a single cell can be exposed to a large set of temperatures and concentrations of chemicals in a short time.Transient receptor potential (TRP) channels are activated by both ligands and temperature, and synergetic effects exist between temperature and concentration of ligand. Therefore, together with patch clamp recording, the microfluidic system is used for studying the response and cooperative effects of temperature and chemicals in TRP channels.Figure 1The microfluidic device contains 16 wells connected to separate channels, with separate outlets in the open volume. In the immediate vicinity of the outlet, there is no mixing between the flows from the different channels due to laminar flow. A thin-film Cr/Au-structure is aligned beneath the channel outlets, which yields resistive heating when a voltage is applied.View Large Image | View Hi-Res Image | Download PowerPoint Slide

Compartmental Modeling of a Structured Heat Exchanger Reactor: Conversion and Temperature Profiles Predictions

Since the last ten years numerous studies have been carried out to develop micro-reactors or micro-structured reactors in order to improve scale of production, economics, safety and environmental impact of chemical production. Consequently Alfa Laval Vicarb has developed a new structured heat exchanger reactor. The design of the reactor is based on high performances heat plate exchanger in which small inserts allow a good mixing of the reactants and to improve the heat transfer phenomena or to increase the heat transfer coefficient. This new technology of heat plate exchange reactors has a modular structure where the exchange zones have two possible configurations: co- and counter counter-current. The structure of the reactor is also flexible, that makes possible to have multiple reactants injections and different local temperature control points. In order to have a rapid prediction of conversion and temperature profiles, a mathematical model conserving the same notion of the structure of the reactor has been developed. This mathematical model is a simple compartmental model which presents advantages in terms of flexibility, possible online simulations and rapid prediction simulations. The structure of the model has been determined with the help of tracer experiment and computational fluid dynamics simulations. Heat and mass transfer equations are written considering each cell and using experimental heat transfer data then chemical kinetics from literature are introduced to the model. A sub-compartmental model has been developed in order to predict the micro-mixing phenomena. Four reactions for which the kinetic laws of reactions are well known have been selected to test the model: alkaline hydrolysis of ethyl acetate, alkaline hydrolysis of ethylene glycol diacetate, oxidation of sodium thiosulphate by hydrogen peroxide and Bourne reactions. The results of comparison between simulations and experimental data in terms of yield, selectivity, temperature profiles and micro-mixing characteristics are in reasonable agreement.

Temperature Control System with Multi-closed Loops for Lithography Projection Lens

Image quality is one of the most important specifications of optical lithography tool and is affected notably by temperature, vibration, and contamination of projection lens(PL). Traditional method of local temperature control is easier to introduce vibration and contamination, so temperature control system with multi-closed loops is developed to control the temperature inside the PL, and to isolate the influence of vibration and contamination. A new remote indirect-temperature-control(RITC) method is proposed in which cooling water is circulated to perform indirect-temperature-control of the PL. Heater and cooler embedded temperature control unit(TCU) is used to condition the temperature of the cooling water, and the TCU must be kept away from the PL so that the influence of vibration and contamination can be avoided. A new multi-closed loops control structure incorporating an internal cascade control structure(CCS) and an external parallel cascade control structure(PCCS) is designed to prevent large inertia, multi-delay, and multi-disturbance of the RITC system. A nonlinear proportional-integral(PI) algorithm is applied to further enhance the convergence rate and precision of the control process. Contrast experiments of different control loops and algorithms were implemented to verify the impact on the control performance. It is shown that the temperature control system with multi-closed loops reaches a precision specification at ±0.006 ℃ with fast convergence rate, strong robustness, and self-adaptability. This method has been successfully used in an optical lithography tool which produces a pattern of 100 nm critical dimension(CD), and its performances are satisfactory.

Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans

Microscopy, phenotyping and visual screens are frequently applied to model organisms in combination with genetics. Although widely used, these techniques for multicellular organisms have mostly remained manual and low-throughput. Here we report the complete automation of sample handling, high-resolution microscopy, phenotyping and sorting of Caenorhabditis elegans. The engineered microfluidic system, coupled with customized software, has enabled high-throughput, high-resolution microscopy and sorting with no human intervention and may be combined with any microscopy setup. The microchip is capable of robust local temperature control, self-regulated sample-loading and automatic sample-positioning, while the integrated software performs imaging and classification of worms based on morphological and intensity features. We demonstrate the ability to perform sensitive and quantitative screens based on cellular and subcellular phenotypes with over 95% accuracy per round and a rate of several hundred worms per hour. Screening time can be reduced by orders of magnitude; moreover, screening is completely automated.

Brain cooling maintenance with cooling cap following induction with intracarotid cold saline infusion: A quantitative model

Intracarotid cold saline infusion (ICSI) is potentially much faster than whole-body cooling and more effective than cooling caps in inducing therapeutic brain cooling. One drawback of ICSI is hemodilution and volume loading. We hypothesized that cooling caps could enhance brain cooling with ICSI and minimize hemodilution and volume loading. Six-hour-long simulations were performed in a 3D mathematical brain model. The Pennes bioheat equation was used to propagate brain temperature. Convective heat transfer through jugular venous return and the circle of Willis was simulated. Hemodilution and volume loading were modeled using a two-compartment saline infusion model. A feedback method of local brain temperature control was developed where ICSI flow rate was varied based on the rate of temperature change and the deviation of temperature to a target (32 °C) within a voxel in the treated region of brain. The simulations confirmed the inability of cooling caps alone to induce hypothermia. In the ICSI and the combination models (ICSI and cap), the control algorithm guided ICSI to quickly achieve and maintain the target temperature. The combination model had lower ICSI flow rates than the ICSI model resulting in a 55% reduction of infusion volume over a 6 h period and higher hematocrit values compared to the ICSI model. Moreover, in the combination model, the ICSI flow rate decreased to zero after 4 h, and hypothermia was subsequently maintained solely by the cooling cap. This is the first study supporting a role of cooling caps in therapeutic hypothermia in adults.

On-chip fabrication to add temperature control to a microfluidic solution exchange system

We present a concept for the post production modification of commercially available microfluidic devices to incorporate local temperature control, thus allowing for the exact alignment of heating structures with the existing features, e.g. wells, channels or valves, of a system. Specifically, we demonstrate the application of programmable local heating, controlled by computerized PI regulation, to a rapid solution exchanger. Characterisation of the system to show that both uniform temperature distributions and temperature gradients can be established, and to confirm that the solution exchange properties are undisturbed by heating, was achieved using in situ thermometry and amperometry.

High-Throughput Synthesis and Screening of Hydrogen-Storage Alloys

Libraries of mixed-metal hydride materials are synthesized on a silicon microfabricated array of "hot-plate" MEMS devices, which allow high-throughput screening using temperature programmed desorption and infrared thermography. The heating plate of the MEMS device is a membrane with low heat capacity, allowing fast and localized temperature control and the extraction of calorimetric data from thermography. The combination of the synthetic method and screening chip allows a fast determination of the desorption temperature and hydrogen content of the materials. Mixed metal hydrides are synthesized directly. The potential of the method is exemplified by presenting results for the sorption properties of Mg xNi 1- x hydride thin-film materials. The results are consistent with the literature, showing the highest hydrogen capacity and desorption temperature for the MgH 2 phase in Mg-rich compositions and the promotion of a lower temperature desorption from the Mg 2NiH 4 phase, with a concomitant reduction in hydrogen capacity.

Plasmon-Assisted Local Temperature Control to Pattern Individual Semiconductor Nanowires and Carbon Nanotubes

We demonstrate a new versatile strategy to rapidly heat and cool subdiffraction-limited volumes of material with a focused light beam. The local temperature rise is obtained by exploiting the unique optical properties of metallic nanostructures that facilitate efficient light-to-heat conversion through the excitation of surface plasmons (collective electron oscillations). By locally heating nanoscale metallic catalysts, growth of semiconductor nanowires and carbon nanotubes can be initiated and controlled at arbitrarily prespecified locations and down to the single nanostructure level in a room-temperature chamber. This local heating strategy can be orders of magnitude (>10(5)) more energy efficient than conventional chemical vapor deposition (CVD) tools in which an entire chamber/substrate is heated. For these reasons, it has great potential for use in process- and energy-efficient assembly of nanowires into complementary metal-oxide-semiconductor (CMOS) compatible device architectures. In general, the high degree of spatial and temporal control over nanoscale thermal environments afforded by this method inspires new pathways for manipulating a range of important thermally stimulated processes and the development of novel photothermal devices.

Micro thermoindicators and optical-electronic temperature control for microfluidic applications

The authors report the design and implementation of sensing and control of local temperature in polydimethylsiloxane microfluidic reaction chip, based on the fabrication of a microtemperature sensor with thermochromic color bars and the associated optical and electronic feedback controls. The thermochromic color bar demonstrates easy and accurate local temperature monitoring. In combination with a microheater, this contactless microchip temperature control approach may have wide application potentials in microchemical and microbiological analyses.

Time-resolved visualization of the heat flow in VO2∕Al2O3 films

The authors performed resistance switching of a VO2 film grown on the Al2O3 substrate by applying electric pulses. As a consequence, Joule heat of about 1W has been induced in a 15×50μm2 area between the two electrodes. Using synchrotron-based infrared microspectroscopy, the authors mapped the propagation of Joule heat around the electrodes with a micrometric spatial resolution and in a submicrosecond time scale. They found that the experimental observations are well described by the Fourier law of heat conduction demonstrating the possibility of local temperature control in a micrometric length scale.

Mitigation and control of the particle pinch in the Electric Tokamak

The Electric Tokamak [R. J. Taylor, T. A. Carter, J.-L. Gauvreau et al., Nucl. Fusion 45, 1634 (2005)] operates at high plasma density (one and a half times the Greenwald limit) due to a strong particle pinch. However, particle accumulation causes several problems. The operation of the machine can suffer several violent disruptions that hinder the study of many plasma phenomena. Plasma motion and large density swings are undesirable because they alter continuous processes, leaving only transient regimes to study. Particle source and local temperature control can defeat the fundamental mechanisms of this ‘‘electric’’ pinch. If edge fueling feedback is not sufficient to induce quiescent behavior, the fast ion loss caused by second harmonic ion-cyclotron rf injection functions as a particle sink deep within the outer plasma cross section. By linking these strong effects to the fueling feedback, stable medium density (2×1018particles∕m3) plasmas can be sustained for several seconds. This new regime yields surprisingly long and calm discharges.

Manipulation of surface reaction dynamics by global pressure and local temperature control: A model study

Specific catalyst design and external manipulation of surface reactions by controlling accessible physical or chemical parameters may be of great benefit for improving catalytic efficiencies and energetics, product yield, and selectivities in the field of heterogeneous catalysis. Studying a realistic spatiotemporal one-dimensional model for CO oxidation on Pt(110) we demonstrate the value and necessity of mathematical modeling and advanced numerical methods for directed external multiparameter control of surface reaction dynamics. At the model stage we show by means of optimal control techniques that species coverages can be adjusted to desired values, aperiodic oscillatory behavior for distinct coupled reaction sites can be synchronized, and overall reaction rates can be optimized by varying the surface temperature in space and time and the CO and O2 gas phase partial pressure with time. The control aims are formulated as objective functionals to be minimized which contain a suitable mathematical formulation for the deviation from the desired system behavior. The control functions pCO(t) (CO partial pressure), pO2(t) (O2 partial pressure), and T(x,t) (surface temperature distribution) are numerically computed by a specially tailored optimal control method based on a direct multiple shooting approach which is suitable to cope with the highly nonlinear unstable mode character of the CO oxidation model.

Volumetric thermal devascularization of large meningiomas

Controlling hemorrhage is crucial in the safe and efficient removal of large meningiomas. Intravascular embolization is not always a satisfactory means of accomplishing this goal because of the procedure's hemostatic effect and risk of complications. The authors in this study used a volumetric thermal ablation technique incorporating radiofrequency energy, image guidance, and local temperature control to devascularize tumor tissue. Five patients with large meningiomas were treated. The target and orientation of the radiofrequency thermal ablation (RFTA) were simulated preoperatively to maximize devascularization of the lesion without thermal injury to adjacent critical structures. Image fusion, three-dimensional reconstruction, and image-guided methods provided for optimized trajectories and targets for insertion of the RFTA needle. During ablation, local temperatures of the tissue being cauterized were monitored continuously to limit the ablated lesion to within the target volume. The effects of devascularization and the softening of the tumor parenchyma facilitated lesion removal. The intracranial ablated meningioma changed into necrotic tissue and shrank within a few months. Histopathological examination of the ablated lesion revealed sharply demarcated coagulation necrosis. Volumetric thermal devascularization can be applied safely in the treatment of large meningiomas to facilitate surgical manipulation of the lesion as well as to reduce its size palliatively. The procedure's usefulness should be studied further in a larger number of cases with different tumor characteristics.

Control of the sharkskin instability in the extrusion of polymer melts using induced temperature gradients

The extrusion of polymer melts is often rate limited by the onset of an elastic surface instability known as sharkskin. Appearance of these surface distortions is generally unacceptable for commercial applications. The desire to forestall the onset of sharkskin to higher output rates has motivated a considerable amount of research to characterize the nature of the instability. In this manuscript, we will present a series of detailed experiments using a custom fabricated extruder and die. By incorporating thermal breaks and precise localized temperature control of the die and barrel, predetermined temperature gradients could be induced across the extrudate. Polymers are typically very poor thermal conductors, and therefore the effects of heating or cooling from a boundary can be designed to only affect the properties of the extrudate very close to the die wall. We will present data correlating the amplitude and frequency of the sharkskin instability to the bulk and die surface temperature as well as the shear rates. The result is a quantitative processing map that characterizes the instability and demonstrates that by modifying the rheology of the polymeric fluid very near the die exit corner, it is possible to suppress or control the sharkskin instability through isolated die heating or cooling. By reformulating our data into Weissenberg and Deborah numbers using the relaxation time evaluated at the wall temperature, we demonstrate that the sharkskin surface instability is dependent only on flow kinematics and viscometric properties of the fluid very near the die wall, a result of the stress singularity present at the die exit, and independent of bulk fluid properties. This technique could conceivably increase the profitability of extrusion processes and be extended to develop precisely-controlled sharkskin for designing specific functionality into extruded surfaces.

An independent, temperature-controllable microelectrode array.

Rapid, localized temperature control and negligible power consumption are key requisites for realizing effective parallel and sequential processing in the miniaturized, integrated biomedical microdevices where temperature-dependent biochemical reactions and fluid flow occur. In this study, an independent, temperature-controllable microelectrode array, with excellent temperature control rates and minimal power consumption, has been developed using microelectromechanical systems technology. The microfabricated array consists of Pt microelectrodes (100-microm diameter), with n-doped polysilicon microheaters (1.4-k Omega resistance), and vacuum-sealed cavities of depth 6.2 microm and diameter 200 microm. The thermal characteristics of each microelectrode were evaluated electrochemically through surface temperature measurements. The large heater power coefficient (2.1 +/- 0.1 degrees C mW(-1)) and the short heating and cooling times (less than 0.2 s for T(0.95)) are consequences of the vacuum-sealed cavities, which facilitate good thermal isolation and low thermal mass. The temperature of each microelectrode is independently controlled by a dedicated microheater, without thermally influencing the adjacent microelectrodes significantly.

Micro Device for Local Temperature Control under Microscope

The device for controlling the temperature locally under a microscope in a short response time, within 3 seconds, is developed. This device is equipped with a temperature controlling micro heater and a micro thermo sensor patterned on a glass plate which can be used for general biochemical assay. Since this device controls the temperature so locally, the total amount of the heat which is needed for temperature controlling is small enough to avoid the negative effects such as defocusing during observation under a microscope. We established the sensor model to calculate the temperature from the measured resistance value and the initial resistance at the roon temperature. Thus, we can avoid the calibration in each sensor. The rotation velocity change of a single F1 molecule, the rotary biomolecular motor, within short response time is successfully observed by controlling the temperature. This is the result that could not be achieved without MEMS technology. This result also shows the applicability of the device to other temperature controlling biochemical assay.

Symptoms prevalence among office employees and associations to building characteristics

A cross-sectional study was performed in eight companies, comprising 32 buildings without previously recognized indoor air problems. Engineers filled in a technical questionnaire on building characteristics, floor surface materials, ventilation, cleaning procedures, heating and cooling. A total of 3562 employees returned questionnaires on individual factors, workload, perceived physical work environment, allergy and symptoms. Frequent symptoms were feeling of fatigue or heavy-headedness, eye irritation, and dry facial skin. Women reported symptoms more frequently than men. Employees with allergy had a 1.8-2.5 times risk of reporting a high score for general, skin, or mucosal symptoms. The risk of a high symptom score increased with daily visual display unit (VDU) work time. Passive smoking and psychosocial load were also relatively strong predictors of symptoms. Weekly cleaning as compared with a frequency of cleaning two to four times a week increased the risk of symptoms. Adjusted odds ratio for a high general symptoms score from infrequent cleaning was 1.5 (95%CI 1.1-2.0). A high ventilation flow or central ventilation unit filter EU7 vs. EU8 seemed to be associated with an increased risk of general symptoms. Absence of local temperature control increased the risk of mucosal symptoms.

Local Temperature Control within a Confined Space by Using a Neural Network Model.

Precision agriculture is now practiced in plant factories. In the case of precision agriculture in Japan, the Microprecision Agriculture program is defined as an ultimate optimized plant production system utilized in order for plant factories to have environmental safeguards that are compatible with yield. Microprecision techniques to manage temperatures or other parameters in the plant factory can provide objectively controlled environments for specific purposes. In fact, in a plant factory, some environmental non-uniformity always exists within the interior. Most of the environmental factors in a fully controlled plant factory are observable and controllable. The conventional strategy for the environmental control of the interior of plant factories has been to make atmospheric factors, such as temperature and humidity, as uniform as possible throughout the interior space. This paper discusses an experiment to control local temperatures, as an example of how microprecision technology can be implemented in plant factories. The results of the experiment using a neural network model show that it is possible to control the temperature distributions by manipulating the wind directions and the temperatures of the airflow. This technique can also be implemented in people's living and working environments.

Local temperature control within a confined space

Precision agriculture is now practiced in plant factories. Microprecision techniques to manage temperature or other parameters in the plant factory can provide objectively controlled environments for specific purposes. This paper discusses an experiment to control local temperatures, as an example of how microprecision technology can be implemented in plant factories. This technique can also be implemented in people's living and working environments

The creation of linear contiguous lesions in the atria with an expandable loop catheter

OBJECTIVESThis article describes a catheter system designed to create linear atrial lesions and identifies electrophysiologic markers that are associated with the creation of linear lesions.BACKGROUNDAtrial fibrillation (Afib) is the most common arrhythmia in humans and causes a significant morbidity. The success of surgical interventions has provided the impetus for the development of a catheter-based approach for the ablation of Afib.METHODSWe tested a catheter system with 24 4-mm ring electrodes that can create loops in the atria. The electrodes can be used to record electrical activity and deliver radiofrequency power for ablation. In 33 dogs, 82 linear lesions were generated using three power titration protocols: fixed levels, manual titration guided by local electrogram activity and temperature control. Bipolar activity was recorded from the 24 electrodes before, during and after lesion generation. Data were gathered regarding lesion contiguity, transmurality and dimensions; the changes in local electrical activity amplitude; the incidence rate of rapid impedance rises and desiccation or char formation; and rhythm outcomes.RESULTSCatheter deployment usually requires <60 s. Linear lesions (12 to 16 cm in length and 6 ± 2 mm wide) can be generated in 24 to 48 min without moving the catheter. Effective lesion formation can be predicted by a decrease of greater than 50% in the amplitude of bipolar recordings. Splitting or fragmentation of the electrogram and increasing pacing threshold (3.1 ± 3.3 mV to 7.1 ± 3.8 mV, p < 0.01) are indicative of effective lesion formation. Impedance rises and char formation occurred at 91 ± 12°C. Linear lesion creation does not result in the initiation of Afib. However, atrial flutter was recorded after the completion of the final lesion in 3/12 hearts. When using temperature control, no char was noted in the left atrium, whereas 8% of the right atrium burns had char.CONCLUSIONSThis adjustable loop catheter forces the atrial tissue to conform around the catheter and is capable of producing linear, contiguous lesions up to 16 cm long with minimal effort and radiation exposure. Pacing thresholds and electrogram amplitude and character are markers of effective lesion formation. Although Afib could not be induced after lesion set completion, sustained atrial flutter could be induced in 25% of the hearts.