Constructing medium–low-speed magnetic levitation (MLS maglev) subgrade by borrowing the construction standards of high-speed railway (HSR) subgrade can result in enormous engineering waste because the amplitude, distribution width, and dynamic coefficient of the dynamic load of MLS maglev trains are much smaller than those of HSR trains. To address this problem, an innovative subgrade structure was proposed to match the dynamic loads of MLS maglev trains. Two experimental models are constructed to study the proposed MLS maglev subgrade at train speeds of 100 km/h, 120 km/h, 160 km/h, and 200 km/h. The two models are labeled as Models I and II, and the only difference between these two models is the type of surface layer material of the subgrade bed, whereas the other structural features are the same. Specifically, the Model Ⅰ employs graded gravel, whereas the Model Ⅱ uses A group filling. The experimental results indicate that for both models, the dynamic earth pressure amplitudes are all less than 20 % of the subgrade’s self-weight at the bottom surface of the subgrade bed. In addition, the cumulative settlement of the two models is below the cumulative settlement limit. Although both models with the proposed MLS maglev subgrade exhibit excellent long-term performance, the Model Ⅰ has superior attenuation characteristics of dynamic earth pressure, and smaller acceleration and dynamic deformation than that the Model Ⅰ. This is attributed to the graded gravel used in the Model Ⅰ, which possesses a greater dynamic modulus and damping ratio than the A group filling used in the Model Ⅱ. The results presented in this study could contribute to the comprehension of the dynamic behavior of the subgrade under the dynamic loads of MLS maglev trains. More importantly, compared to the existing MLS maglev subgrades, the proposed MLS maglev subgrade has lower construction standards and is thus suitable for practical engineering applications.