Livestock Wastewater Treatment Research Articles

Response of denitrobacteria involved in nitrogen removal for treatment of simulated livestock wastewater using a novel bioreactor

A ceramic filter medium (CFM) and dewatered aluminum sludge (DAS) were used as a small-scale combined biofilter to treat synthetic livestock wastewater. The average removal efficiency of ammonia nitrogen (NH4+-N) and total nitrogen (TN) reached 95.75 and 75.11% respectively. Illumina sequencing indicated that, except for initial media, most beta diversities of napA, narG, nirS, qnorB, and nosZ in the CFM or DAS were in a steady-state condition regardless of space-time variation, while these genes showed discrepancy between the CFM and DAS. In addition, the dominant genera were principally influenced by the synergic environmental factors, namely NH4+-N, TN, chemical oxygen demand (COD), and pH of effluents. This research demonstrated that the elimination of TN for treating synthetic livestock wastewater could be achieved by increasing the relative abundance of Azoarcus, Rhodanobacter, and Burkholderia. Meanwhile, increasing these genera contributes to suppressing the genera disadvantageous to TN attenuation.

Formulation and Characterization of a Heterotrophic Nitrification-Aerobic Denitrification Synthetic Microbial Community and its Application to Livestock Wastewater Treatment

There have been many studies on single strains in wastewater treatment and a new synthetic microbial community was prepared in this study, which provides a reference for the application of heterotrophic nitrification-aerobic denitrification in actual wastewater treatment. The growth period distribution of the composite bacteria was determined by plotting growth curves with different sole nitrogen sources, and the influence of the carbon source, carbon to nitrogen ratio (C/N) ratio, pH, and temperature on ammonia removal by the composite heterotrophic nitrifying-aerobic denitrifying strain was investigated. The optimal conditions for the heterotrophic nitrification process were sodium citrate as the carbon source, a C/N ratio of 10, a pH of 7, and a temperature of 30 °C, and only trace amounts of nitrate and nitrite were observed during the process. When the sequencing batch reactor (SBR) of a pig farm wastewater treatment plant was inoculated with the synthetic microbial community, the average removals of the chemical oxygen demand (COD) and ammonia nitrogen in the effluent were 92.61% and 20.56%, respectively. From the results, the synthetic microbial community was able to simultaneously perform heterotrophic nitrification-aerobic denitrification indicating great potential for full-scale applications.

Understanding Antibiotic Residues and Pathogens Flow in Wastewater from Smallholder Pig Farms to Agriculture Field in Ha Nam Province, Vietnam.

Background:Contact with livestock wastewater on farms and in communities can pose a risk to human and animal health.Methods:This cross-sectional study was conducted in 180 households and 24 pig farms (96 wastewater samples) to explore information about pig production, livestock waste management, antibiotic use, and to analyze antibiotic residues and microbial contamination, respectively.Results:Of the 120 households raising pigs, biogas systems were the most commonly used to treat animal waste (70%), followed by compositing (19%), and the remaining respondents discharged waste directly into drains or ponds (11%). The majority of respondents (78%) used antibiotics to treat and prevent disease in pigs, but 32% of them did not know of any disadvantages of antibiotic abuse. ELISA assays were performed on half of the wastewater samples (n = 48), demonstrating that residues of flouroquinolones and sulfonamides were present in 6.3% (3/48) and 22.9% (11/48) of tested samples, respectively. The average residual level of sulfamethazine was 27.8 ug/l. Further, E. coli concentrations exceeding regulatory levels in Vietnam were found in nearly all samples. Salmonella spp. was also found in 57.3% of samples, though prevalence rates varied across the different sites. Finally, G. lamblia was found in 8.4% of samples, and C. parvum was found in 5.2% of samples.Conclusions:This study suggests that livestock wastewater carried potential harmful pathogens and antibiotic residues that could come into contact with humans in the community. Thus, appropriate operation and application of livestock wastewater treatment (such as biogas or composting) and management should be a continued focused.

Contamination of Metal Elements in Livestock Wastewater Treatment Plants in Korea

Contamination of Metal Elements in Livestock Wastewater Treatment Plants in Korea

Removal of Cypermethrin and Chemical Oxygen Demand from Livestock Wastewater by Electrocoagulation

AbstractThe direct discharge of wastewater from livestock into freshwater and surface runoff originating from processing areas threaten aquatic ecosystems and the quality of drinking water taken from streams. The present work studied the removal of chemical oxygen demand (COD) and cypermethrin (Cyp) from livestock wastewater generated after cattle baths by a continuous electrocoagulation (EC) process. The effect of pH, retention time, and voltage on COD and Cyp removal was examined. Under the optimal operating conditions, EC effectively removed COD (93 %) and cypermethrin (96 %). Thus, continuous EC was successfully applied to treat livestock wastewater.

Antibiotics in livestock wastewater treatment by using biomass-derived activated carbon supported ZnS nanomaterials.

A new type of composite photocatalyst material was successfully prepared through the ultrasound-assisted coprecipitation method precipitate of zinc sulfide (ZnS) nanomaterials on peach wood activated carbon (PAC). The optimization of ZnS@PAC demonstrates excellent photocatalytic performance by using the response surface method (RSM), which is essential for improving photocatalytic performance. In this model it was found that the photocatalytic degradation of enrofloxacin (ENR) increased with microwave heating power and ZnS concentration, whereas it decreased with increasing activation time. The RSM model predicts that under certain conditions (microwave heating power 800 W, activation time 3 h, ZnS 0.5 mol·L-1), the maximum degradation rate of ENR in livestock and poultry wastewater is 97.81%. By empirical testing under the optimum conditions with 97.35% degradation the accuracy of the designed model was proven using RSM and the mechanism of the photocatalytic process was studied.

Removal of tetracycline via the synergistic effect of biochar adsorption and enhanced activation of persulfate

We developed a simple in-situ system to remove model antibiotic tetracycline (TC) using biochar (BC) adsorption and persulfate (PS) oxidation (Cu/BC/PS). The biochar produced by pyrolysis of corn stalks at 700 °C exhibited a much higher Brunauer-Emmett-Teller (BET) surface area (431 m2/g), absorption capacity of Cu2+ (8.37 mg/g) and tetracycline (18.80 mg/g) than that of other temperature-prepared biochar. The removal efficiency of TC with the Cu/BC700/PS system reached 55.4%–72.6% with solution conditions of pH 4–9, 120 mg/L TC, 300 mg/L PS and 0.5 g/L BC. Under 619 ± 5 mg/L chemical oxygen demand (COD) condition, the TC removal efficiency of the Cu/BC700/PS system was about 5 times more than that of the Cu/PS system. The results of characterization methods such as Electron paramagnetic resonance (EPR), X-ray photoelectron spectroscopy (XPS), and radical quenching experiments suggested that the reduced Cu(I) on biochar was a catalytic center in the degradation process and the SO4·- and OH were the dominant radicals. The reaction kinetics showed that the BC adsorption of Cu2+ and TC were the limiting steps of the entire reaction. A proposed system mechanism is that Cu is absorbed by the biochar and helps further enhance the activation of PS. In the practical application to biogas slurry (2383 ± 47 mg/L COD), the total removal efficiencies of 5 heavy metals and 15 antibiotics were 57.4% and 42.3%, respectively. Moreover, the germination index (GI) of the biogas slurry increased from 8.50% to 43.48% after treatment. Thus, the Cu/BC/PS system has a great prospect in the harmless treatment and recycling of livestock wastewater.

Novel electrodes for cathode electro-Fenton oxidation coupled with anodic oxidation system for advanced treatment of livestock wastewater

With the rapid development of livestock industry, a large amount of livestock wastewater generates in China in recent years. The conventional treatment process for livestock wastewater, including anaerobic digestion and anoxic-oxic treatment, is very common in China for the low operational cost. However, the very low BOD5/CODCr ratio (<0.1) in the associated effluent renders the further treatment process very difficult. In this study, a PbO2 anode coupled with a 2-ethylanthraquinone (EAQ)-modified graphite felt cathode was used to treat the livestock wastewater (BOD5/CODCr < 0.1) discharged from the conventional two-stage anoxic/oxic (A/O) process. The results of scan electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and fourier transforminfrared spectroscopy (FTIR) show that the prepared PbO2 electrode has a smaller crystal size, higher oxygen evolution reaction (OER) potential and an excellent electro-catalytic performance compared to a purchased PbO2 electrode, and the EAQ-modified graphite felt cathode has a higher content of oxygen-containing functional groups and higher oxygen reduction reaction (ORR) electro-catalytic performance than pristine graphite felt. The results of the electrolysis experiment showed that the BOD5/CODCr ratio was increased from 0.084 to 0.42 with 71.5% CODCr removal after 80 min of electrolysis, when the Fe2+ concentration was 0.3 mM. The .OH produced in the electrolysis system was captured successfully by electron paramagnetic resonance (EPR).

Mixture of different Pseudomonas aeruginosa SD-1 strains in the efficient bioaugmentation for synthetic livestock wastewater treatment

Strains selection for inoculation is the key to the successful construction of a bioaugmentation system, a promising strategy for specific pollutant removal. Pseudomonas aeruginosa SD-1 wild-type (WT) strain exhibited high capacity for biofilm formation but low efficiency for nitrate (NO3−) removal. Meanwhile, quorum sensing deficient strain ΔlasR showed excellent efficiency for NO3− removal but poor capability for colonization in activated sludge. The opposite effect of biofilm formation and NO3− removal exist in WT or ΔlasR, which limits the construction of bioaugmentation system of strain SD-1 and its application. To solve this issue, a mixture of WT and ΔlasR (v/v = 1:1) was used to construct a bioaugmentation system. Compared with the inoculation of WT or ΔlasR alone, the mixed inoculation not only was beneficial for activated sludge development but also for pollutant removal. The indicators for activated sludge including the abundance of P. aeruginosa, the sludge volume index and the average particle size in mixed inoculated reactors were close to those of reactors with single and repeated inoculation of WT. The effluent of chemical oxygen demand (COD) and NO3−-N were stable at 3.9–22.6 mg L−1 and 0–5.53 mg L−1 after d 3, respectively. This study presents a detailed case on the ecological tradeoff of colonization and pollutant removal of inoculated strains during bioaugmentation. The results provide information on the appropriate conditions for application of P. aeruginosa SD-1 for livestock wastewater treatment and further enrich our ecological understanding of bioaugmentation.

A structural equation model to predict macroinvertebrate-based ecological status in catchments influenced by anthropogenic pressures

A Partial Least Squares-Path Model (PLS-PM) was developed for the Ave River Basin (North of Portugal), and the results used in a scenario analysis. The data for PLS-PM comprised a set of anthropogenic pressures, water quality parameters, and a macroinvertebrate-based biodiversity index (IPtIN) used to assess the ecological status of streams. These groups of measured parameters (called latent variables) were given the names “Pressures”, “Contamination” and “Ecological Integrity”. Besides, latent variables were connected through path coefficients representing potential causal effects among them. In a large portion of Ave the ecological status of streams is currently bad or poor. Nitrate and coliforms were the most weighted measured variables of latent variable “Contamination”, with w ≈ 0.7 and w ≈ 0.2, respectively. The highest “Pressures” weights were ascribed to livestock farming (0.7) and population density (0.4). The connections “Pressures”—“Contamination” and “Contamination” — “Ecological Integrity” exposed a sequence of direct negative effects between the three variables, expressed in the corresponding path coefficients (pc = 0.87 and pc = −1.11). Paradoxically, a direct negative effect of “Pressures” over “Ecological Integrity” was absent (pc = 0.29). Therefore, the poor ecological status of local stream waters might not be directly related to the presence of potentially threatening contaminant sources (the “Pressures”), but to ineffective monitoring of livestock farming and wastewater treatment activities that potentiate (accidental) releases of contaminants into the streams. The lack of a direct link “Pressures” — “Ecological Integrity” supported the results of pressure change versus IPtIN change scenarios. Regardless of some significant reductions of anthropogenic activity and population density until 2027, announced by the Portuguese Environmental Agency, the scenarios could not predict improvement of ecological status beyond the “moderate” category. The study recommendations were therefore to prevent contamination through proper implementation and monitoring of existing watershed management plans. The adequate treatment of domestic effluents and the control of livestock farming residues are urgent.

Effects of roxarsone and sulfadiazine on biogas production and their degradation during anaerobic digestion

Roxarsone and sulfadiazine are typical feed additives and often occur in manure and livestock wastewater. Anaerobic digestion is a common technique for the treatment of manure and livestock wastewater. However, the influence of roxarsone and sulfadiazine on anaerobic digestion and their degradation are still unclear. In this study, the effect of roxarsone and sulfadiazine on the anaerobic digestion of synthetic wastewater and their degradation were investigated. During 70-day incubation, the presence of 200 mg L−1 sulfadiazine exhibited little influence on hydrolysis and acidification, but resulted in a temporary inhibition on methanogenesis, whereas 120 mg L−1 of roxarsone resulted in nearly complete inhibition on methanogenesis. Further, the co-existence of 100 mg L−1 sulfadiazine and 60 mg L−1 roxarsone significantly exacerbated the inhibition on anaerobic digestion. Sulfadiazine exhibited high resistance to the adsorption and biodegradation, while roxarsone was nearly completely degraded during anaerobic digestion. Nearly half of total arsenic was transferred from solution to anaerobic granular sludge. The predominant arsenic species was 4-hydroxy-3-aminophenylarsonic acid in the solution and As3+ in the sludge. These results will provide valuable information for anaerobic treatment of livestock wastewater simultaneously contaminated by roxarsone and sulfadiazine.

Carex paniculata constructed wetland efficacy for stormwater, sewage and livestock wastewater treatment in rural settlements of mountain areas

Constructed wetlands are one of the most appropriate wastewater treatment systems in mountain areas, where altitude, slope and climate constitute major environmental and economic constraints for infrastructure construction and subsequent management. In order to protect mountain natural wetland habitats that are sensitive to ecological equilibrium disruption, instead of the more commonly used macrophytes, plant species native to upland wetlands should be preferentially implemented as a contribution to biodiversity conservation and for the creation of more efficient, more resilient and better-adapted constructed wetlands. Carex paniculata is a key macrophyte in several European mountain aquatic habitats, and one of the few high-biomass producers that can grow at sea level and at altitudes of up to 2,600 m. In this paper, the results of a 2-year investigation demonstrate the efficacy of Carex paniculata for the treatment of the mixed stormwater, sewage and livestock wastewater effluents from a typical rural settlement at 825 m above sea level in the Cantabrian Mountains. The year-round suitability of Carex paniculata for the treatment of wastewater with seasonally variable flow and composition in mountain areas is demonstrated.

Removal of veterinary antibiotics in constructed wetland microcosms – Response of bacterial communities

This study aimed to evaluate the response of bacteria, in terms of microbial community structure, from constructed wetland (CW) microcosms exposed to two veterinary antibiotics, enrofloxacin (ENR) and ceftiofur (CEF), alone or in a mixture, identifying which bacterial groups were dominant in CWs substrate during livestock wastewater treatment. Wastewater, not-doped or doped with ENR and/or CEF (100 µg/L each), was treated during 18 one-week cycles. Systems showed removal percentages > 85% for the added antibiotics, showing also high removal percentages for nutrients and organic matter and confirming CWs systems were working properly. However, both time of exposure and presence of antibiotics influenced significantly CWs substrate bacterial community structure. Pyrosequencing results showed bacterial communities were dominated by phyla Proteobacteria (38–48%), Firmicutes (20–27%), Bacteroidetes (12–15%) and Actinobacteria (4–9%), and that their relative abundance was clearly affected by the presence of the antibiotics. Results suggest the applicability of CWs for the removal of veterinary antibiotics from livestock wastewaters and provide new knowledge about the bacteria within the system, which can potentially be involved in removal processes. This information could in the future be used to improve CWs removal rates of pharmaceuticals from livestock wastewaters.

Association of robust nitrogen removal with spatiotemporal nitrifying bacterial community dynamics in a new bioreactor for treatment of simulated livestock wastewater with high ammonia content

AbstractBACKGROUNDDifferent types of nitrogen‐containing wastewater have been treated with dewatered alum sludge (DAS) or neutralized used acid (NUA) or with a combined DAS–NUA biofilter. The connections between the spatiotemporal structures of ammonia‐oxidizing bacteria (AOB) and nitrite‐oxidizing bacteria (NOB) and the nitrogen removal performance of DAS–NUA biofilter have not been fully clarified. To address this issue, AOB and NOB genetic characteristics response to nitrogen conversions in a laboratory‐scale DAS–NUA biofilter were investigated.RESULTSThe spatiotemporal distribution of the AOB in DAS was heterogeneous in the bioreactor. The trends observed in its structure were a function of depth along the flowpaths and time. However, AOB in NUA and NOB in both substrates were time and space independent. The spatial and temporal behavior of nitrifying bacteria mainly depended on the pH, followed by dissolved oxygen. The Shannon indexes of AOB and NOB and the relative abundances of Nitrosomonas and Nitrobacter had significantly negative relationships with NH+4–N and total nitrogen concentrations in effluents.CONCLUSIONSThe Shannon indexes of AOB and NOB and the relative abundances of Nitrosomonas and Nitrobacter were the key indicators of nitrogen removal and have implications for effective nitrogen attenuation strategies. © 2018 Society of Chemical Industry

C:N ratio shaped both taxonomic and functional structure of microbial communities in livestock and poultry breeding wastewater treatment reactor

C:N ratios play critical roles in determining the stability and performance of the wastewater treatment reactor. Here, we investigated bacterial and archaeal community composition, diversity, association networks, and functional profiles in livestock and poultry breeding wastewater (LPBW) with C:N gradients from 7.8 to 18.9 using 16S rRNA gene amplicon sequencing. Highest total nitrogen (TN) and total phosphorous (TP) removal rates were detected in the wastewater with high C:N ratios, while bacterial and archaeal communities in the wastewater varied across the four C:N ratios. Proteobacteria, Acidobacteria, and Bacteroides were generally the dominant phyla in the wastewater across treatments, with Candidatus Saccharibacteria being more enriched in the wastewater with high C:N ratios. Association network analysis showed that specific bacterial and archaeal taxa likely have similar metabolic activities allowing them to respond similarly to different C:N ratios. Bacteroidetes, Actinobacteria, Verrucomicrobia, Candidatus Saccharibacteria, and Proteobacteria were the keystone species found in the networks. Most dominant bacterial functions in the wastewater were chemoheterotrophy and aerobic chemoheterotrophy. Nitrite respiration, nitrous oxide denitrification, nitrate denitrification and nitrite denitrification were up-regulated with increased C:N ratios. Our findings provide new insights into our understanding of the compositions, potential associations, and predicted functional profiles of the microbial community in LPBW treated with different C:N ratios.

Study on Nitrification Characteristics of Livestock Wastewater Treated With SBR

By coupling intermittent characteristics of livestock wastewater and intermittent operation of SBR, the feasibility of using intermittent aeration to reduce energy consumption of livestock wastewater treatment facilities was studied. SBR was utilized to treat livestock wastewater pretreated by the anaerobic process. A systematic study of the start-up of nitrification in SBR using the novel aeration method and the influence of aeration rate and aeration time on nitrification performance was carried out. The results showed that the removal of NH4+-N could be achieved under different operating conditions. DO concentration was the dominant factor affecting the nitrification process. Under the operation condition of stage 3 (i. e. aeration rate 0.8 L/min, temperature 25 °C, aeration time 12 h). Because of the lack of carbon source, the removal rate of TN decreased, and a certain degree of accumulation of NO3--N occurred. Through monitoring the DO, pH and Eh, the results indicated that the Eh curves varied irregularly. However, DO and pH in one operating cycle showed obvious patterns and corresponded well with the nitrification process, so they could be used as real-time control parameters to control the nitrification aeration process.

Antibiotic resistance genes and intI1 prevalence in a swine wastewater treatment plant and correlation with metal resistance, bacterial community and wastewater parameters

The livestock wastewater treatment plant represents an important reservoir of antibiotic resistance determinants in the environment. The study explored the prevalence of five antibiotic resistance genes (ARGs, including sulI, tetA, qnrD, mphB and mcr−1) and class 1 integron (intI1) in a typical livestock wastewater treatment plant, and analyzed their integrated association with two metal resistance genes (copA and czcA), two pathogens genes (Staphylococcus and Campylobacter), bacterial community and wastewater properties. Results indicated that all investigated genes were detected in the plant. The treatment plant could not completely remove ARGs abundances, with up to 2.2 × 104~3.7 × 108 copies/L of them remaining in the effluent. Mcr−1 was further enriched by 27-fold in the subsequent pond. The correlation analysis showed that mphB significantly correlateed with tetA and intI. Mcr−1 strongly correlated with copA. MphB and intI significantly correlated with czcA. The correlations implied a potential co-selection risk of bacterial resistant to antibiotics and metals. Redundancy analyses indicated that qnrD and mcr−1 strongly correlated with 13 and 14 bacterial genera, respectively. Most ARGs positively correlated to wastewater nutrients, indicating that an efficient reduction of wastewater nutrients would contribute to the antibiotic resistance control. The study will provide useful implications on fates and reductions of ARGs in livestock facilities and receiving environments.

재활용 건축 폐기물을 이용한 축산폐수처리 혐기성 소화조 발생 황화수소 제거 연구

재활용 건축 폐기물을 이용한 축산폐수처리 혐기성 소화조 발생 황화수소 제거 연구

Semi-continuous operation and fouling characteristics of submerged membrane photobioreactor (SMPBR) for tertiary treatment of livestock wastewater

Abstract A submerged membrane photobioreactor was tested to provide a feasible means to conduct tertiary treatment of livestock wastewater. In this study, three phases of a semi-continuous photobioreactor were tested based on hydraulic retention time to evaluate biomass productivity, effluent quality, and fouling characteristics of the membrane module. The results show that the membrane module contributes to securing effluent quality with high nitrogen (36%) and phosphorus (25%) daily removal rates. Shorter hydraulic retention time resulted in better performance for removal of nitrogen and phosphorus; a 3 d hydraulic retention time resulted in 96% (nitrogen), and 85% (phosphorus) removal. Under the proposed condition, the biomass concentration simultaneously increased to 3500 mg/L (dry weight). Active photosynthesis in the photobioreactor was strongly associated with the average concentrations of soluble microbial products (carbohydrate: 84.5 mg/L, protein: 37.9 mg/L) and extracellular polymeric substances (carbohydrate: 44.1 mg/L, protein: 556.9 mg/L). Relatively high carbohydrate concentrations in the permeate provided evidence of a strong relationship between the effluent quality and carbohydrates in the soluble microbial products. Morphological and chemical analyses proved that micro-algal membrane fouling is attributable to soluble microbial products and/or extracellular polymeric substances. These occur in significant amounts after 10 d, indicating an unavoidable need for biomass harvesting. This study provides an understanding of the fouling trend according to varying hydraulic retention time and biomass harvesting, and offers a viable operation strategy for use of a submerged membrane photobioreactor to convert pollutants into useful bioresources and improve effluent quality.

IbM KELOMPOK PETERNAK SAPI PERAH Dl BENDUL MERISI KELURAHAN BENDUL MERISI, KECAMATAN WONOCOLO, KOTA SURABAYA

Potential products fresh milk from a partners dairy farmers in the area Bendul Merisi Surabaya is still quite favorable, but the technology has not been used in terms of addressing the problem of livestock waste, so the existence of the business partners still largely very simple in handling the waste, which could impact unhealthy society around. The existence of farm businesses that are geographically dense settlements which are urban areas in Surabaya, where villagers are a mixture of local women for newcomers. Required touch technology and knowledge in the group of dairy farmers are still traditional, thus providing added value to the farm business that is expected to have an impact on the surrounding environment healthier. In the implementation of community service (PPM) is made to partners I, namely Mr. Masoud located in the Bendul Merisi, 27 Surabaya and partners II, Mrs. Aslam in the Bendul Merisi, 31 Surabaya, both within the village RW.6 RT.5 Bendul Merisi Wonocolo Subdistrict Surabaya. Priority issues in general partners after advocacy by Tim PPM states have realized the importance of livestock wastewater treatment so as not to impact negatively on the environment and understand the importance of fresh milk production management by developing a broader market access (internet media). The purpose of this devotion 1). provide a good understanding of the handling of liquid waste from the farm; 2). provide a good understanding of marketing management of fresh dairy products; 3). develop household business partners who are economically independent. The method is offered for problems of waste and management are 1) helping to prepare the handling and management of liquid waste effectively is by building bathtub tiered so that the water coming out of the location of the farm is quite clean and does not pollute the environment, 2) help increase the marketing management product by utilizing Internet access. By the conclusion of 2016 IbM have done the steps are 1) the innovation of science and technology in the form of construction of wastewater treatment basin farms in stages; 2) advocacy to partner in improving the understanding of appropriate technology and 3) to facilitate the implementation of Internet technology to expand its internet blog marketing information and business networks. With these steps, the partners feel the positive benefits such as ease of access to fresh milk sales and the emergence of consciousness and moral responsibility for pollution of river water in view of the farm is located in a densely populated urban environment Surabaya. DOI: https://doi.org/10.26905/abdimas.v2i2.1811