Mixture of different Pseudomonas aeruginosa SD-1 strains in the efficient bioaugmentation for synthetic livestock wastewater treatment

Abstract

Strains selection for inoculation is the key to the successful construction of a bioaugmentation system, a promising strategy for specific pollutant removal. Pseudomonas aeruginosa SD-1 wild-type (WT) strain exhibited high capacity for biofilm formation but low efficiency for nitrate (NO3−) removal. Meanwhile, quorum sensing deficient strain ΔlasR showed excellent efficiency for NO3− removal but poor capability for colonization in activated sludge. The opposite effect of biofilm formation and NO3− removal exist in WT or ΔlasR, which limits the construction of bioaugmentation system of strain SD-1 and its application. To solve this issue, a mixture of WT and ΔlasR (v/v = 1:1) was used to construct a bioaugmentation system. Compared with the inoculation of WT or ΔlasR alone, the mixed inoculation not only was beneficial for activated sludge development but also for pollutant removal. The indicators for activated sludge including the abundance of P. aeruginosa, the sludge volume index and the average particle size in mixed inoculated reactors were close to those of reactors with single and repeated inoculation of WT. The effluent of chemical oxygen demand (COD) and NO3−-N were stable at 3.9–22.6 mg L−1 and 0–5.53 mg L−1 after d 3, respectively. This study presents a detailed case on the ecological tradeoff of colonization and pollutant removal of inoculated strains during bioaugmentation. The results provide information on the appropriate conditions for application of P. aeruginosa SD-1 for livestock wastewater treatment and further enrich our ecological understanding of bioaugmentation.