Levofloxacin Lactate Research Articles

Pharmacovigilance of cutaneous adverse drug reactions in associations with drugs and medical conditions: a retrospective study of hospitalized patients

BackgroundCutaneous adverse drug reaction (CADR) is a common problem in clinical medication. This study aimed to investigate the correlation between clinical drug application and CADR occurrence as evidence for preventive strategies and rational clinical drug use.MethodsWe analyzed the characteristics of CADRs of 858 patients admitted to Shandong Provincial Third Hospital from March 2007 to December 2018. The most significant drugs concerning the common skin symptoms and their significance to CADR were investigated by case-non-case and multiple logistic regression analyses.ResultsA total of 266 drugs were involved in 858 cases of CADR. Among the ten most relevant medications, primarily antibiotics and herbal injections, and nutritional support drugs, potassium sodium dehydroandrographolide succinate injection, and cefoperazone sodium and sulbactam sodium injection were found to be 2.1 and 1.45 times statistically more prone to CADRs than to other adverse drug reactions (ADRs), respectively. The main route of administration was intravenous (63.16%), with oral administration accounting for 25.19%. There were 747 cases of ADR, 71 of severe ADR, 2 of new and severe ADRs, and 38 cases of new ADR. Overall, 100 cases of CADR exhibited abnormal alanine aminotransferase, aspartate aminotransferase, and serum creatinine levels. The predictive factors for severe CADR occurrence included allergy and smoking histories, cefoperazone sodium, sulbactam sodium injection, levofloxacin lactate and sodium chloride injection.ConclusionsDrug-induced CADR symptoms are commonly associated with other ARDs, predominantly rashes and pruritus, and are often accompanied by some medical conditions, especially liver and kidney damage. Detailed attention to a patient’s primary diseases, allergy history, and drug safety profile could help prevent or reverse CADR in most patients.

Impact of Different Antibiotic Regimens on Patients with Respiratory Tract Infection

To assess impact of different antibiotic regimens on patients with respiratory tract infection, we establish a randomized controlled trial for comparison of different antibiotic regimens. From May 2019 to February 2021, we collected valid data from 182 patients in the hospital. This study consisted of 3 steps. First, we invited the patients with respiratory tract infection who meet the inclusion criteria. Also, patients were divided into control group (n=98) and intervention group (n=84) according to the antibiotic regimen they received. Second, we collected the data by questionnaires, including basic patient characteristics, treatment period, treatment cost, and treatment effect. Third, we analyze the data by software. In this study, the less days of treatment period and less payment of treatment in intervention group patient than control group patients, that their results between were significantly different. Also, treatment effect of two antibiotic regimens were similar, that the rates of treatment effective were no significantly different. In summary, we found that the patients who received levofloxacin lactate sodium chloride injection treatment had shorter treatment period and less treatment cost than the patients who received ceftazidime treatment, but their treatment effects were similar.

Clinical study of the treatment principle of Yangyin-Huayu for the patients with recurrent hemoptysis of bronchiectasis

Objective To observe the clinical curative effect and the serum CPR changes by the treatment principle of Yangyin-Huayu for the patients with recurrent hemoptysis of Bronchiectasis. Methods A total of 64 patients were enrolled and divided into 2 groups according to the random number table method, 32 patients in each group. The control group was treated with the injection of blood clotting enzyme, pituitrin, levofloxacin lactate. And the treatment group was treated with the Yangyin-Huayu on the basis of the control group. Two groups were treated for 10 days. The CPR was detected by the double optical path immune turbidity analyzer. And the clinical effects, symptoms and signs scores were compared between before and after treatment and between two groups. Results Compared with the total effective rate of control group 84.38%(27/32), the treatment group was 96.88% (31/32) and significantly high (χ2=6.372, P<0.01). After treatment, the levels of serum CPR (2.45 ± 1.42 mg/L vs. 6.19 ± 2.34 mg/L, t=4.671) in the treatment groupwas significantly lower than that in the control group (P<0.01). The scores of cough (0.62 ± 0.91 vs. 1.34 ± 0.58, t=5.364), sputum (0.14 ± 0.28 vs. 0.68 ± 0.72, t=4.035), haemoptysis (0.10 ± 0.65 vs. 0.62 ± 0.26, t=5.012), fever (0.04 ± 0.31 vs. 0.76 ± 0.07, t=3.026), breathing difficulties (0.12 ± 0.41 vs. 0.69 ± 0.05, t=4.010) in the treatment group were lower than those in the control group (P<0.01). Conclusions The treatment principle of Yangyin-Huayu combined with conventional western medicine therapy can improve the patients with recurrent hemoptysisin the fields of symptoms, signs, the serum level of CPR, and total effect rates. Key words: Bronchiectasis; Hemoptysis; Hemostatics (TCD); Removing blood stasis for hemostasis; Nourishing Yin and hemostasis; Integrated Chinese traditional and western medicine therapy

In vivo Raman measurement of levofloxacin lactate in blood using a nanoparticle-coated optical fiber probe.

Monitoring drug concentrations in vivo is very useful for adjusting a drug dosage during treatment and for drug research. Specifically, cutting-edge "on-line" drug research relies on knowing how drugs are metabolized or how they interact with the blood in real-time. Thus, this study explored performing in vivo Raman measurements of the model drug levofloxacin lactate in the blood using a nanoparticle-coated optical fiber probe (optical fiber nano-probe). The results show that we were able to measure real-time changes in the blood concentration of levofloxacin lactate, suggesting that this technique could be helpful for performing drug analyses and drug monitoring in a clinical setting without repeatedly withdrawing blood from patients.

Thermo-responsive hydrogels with N-isopropylacrylamide/acrylamide interpenetrating networks for controlled drug release

Series of thermo-sensitive hydrogels (PNAs) based on N-isopropylacrylamide/acrylamide interpenetrating polymer networks were synthesized via in situ free-radical polymerization. Poly (ethylene glycol diacrylate) and poly (ε-caprolactone diacrylate) were synthesized as macro-cross-linkers due to their excellent biocompatibilities. The macro-cross-linkers and hydrogels were characterized by 1H NMR and FT-IR, respectively. The interior morphology of the hydrogels was observed by scanning electron microscopy. The swelling ratios at different temperatures and the swelling/deswelling kinetics of the hydrogels were studied. Their volume phase transition temperatures were also measured by differential scanning calorimetry characterization. The results indicated that the PNA hydrogels had uniform macroporous structures, and they not only had considerable swelling ratios, but also exhibited rapid swelling/deswelling kinetics and response sensitivities. In addition, the weight ratio of AAm/NIPAAm also affected the swelling performance and phase transition temperature of hydrogels, and its value less than 5% was the optimal proportion to achieve excellent comprehensive properties. Levofloxacin lactate and Naproxen were selected as drugs and simulated in vitro condition release, and the drug release results showed that the PNA hydrogels behaved fast release performance.

Novel fast thermal-responsive poly(N-isopropylacrylamide) hydrogels with functional cyclodextrin interpenetrating polymer networks for controlled drug release

Thermal-responsive hydrogels poly[(N-isopropylacrylamide)-co-(aminoethyl methacrylate β-cyclodextrin)] (PNiCD) were synthesized, and the morphology, thermal behavior and swelling/deswelling kinetic of the PNiCD hydrogels were studied. The results indicate that PNiCD hydrogels not only had considerable swelling ratios and rapid swelling/deswelling kinetics, but also behaved with fast thermal response sensitivities. In addition, by adjusting the weight ratio of GCD/NIPAAm ratio PNiCD hydrogels with different macroporous and thermal-responsive performances can be prepared for potential application in the biological medicine field. Levofloxacin lactate (LL) and rhodamine B (Rh) were elected as model drugs to explore controlled drug release for PNiCD hydrogels. The results showed that about 30 % of the LL and Rh were released within the first 30 min, and 80 % drugs were released at around 300 min, and this release process was faster than that of PNIPAAm hydrogel. The fast release performance indicated that these new CD modified PNIPAAm-based PNiCD hydrogels are promising for use in rapid drug delivery in the biological medicine field.

A non-invasive method for the determination of liquid injectables by Raman spectroscopy.

Drug safety has become a very important subject, and more countries have joined in the fight against counterfeit drugs. This study demonstrated a non-invasive Raman spectroscopy method that could be utilized for screening liquid injectable drugs for spurious/falsely-labeled/falsified/counterfeit medical products (SFFCs). Two problems were solved to remove the blocks in identification and quantitation: one problem was the weak API signal extraction from the non-invasive Raman spectra and the other was the problem of Raman absolute measurement. Principal component analysis (PCA) and classical least square (CLS) algorithms were performed to establish the models. Water was chosen as the "internal standard" to normalize the spectra to solve the problem of Raman absolute measurement. The results showed that the 11 positive samples and 66 negative samples were all well identified with a threshold of 0.95. One of the positive samples contained the excipient propylene glycol, which was identified successfully at the same time. The accuracy of quantitative results was approximately 5% for doxofylline liquid injectables and about 10% for the low-concentration and big glass bottle-containers of Levofloxacin Lactate and Sodium Chloride Injections as compared to the results using an HPLC method, this is satisfactory for fast screening of SFFCs. In conclusion, with the development of a database of identification and quantitation models, this method may determine liquid injectable drugs in a fast and non-invasive way and become one of the most powerful weapons against SFFCs. Graphical Abstract ᅟ.

Raman spectroscopy measurement of levofloxacin lactate in blood using an optical fiber nano‐probe

Liquid chromatography and mass spectrometry were time‐consuming and expensive as the main methods for the drug analysis at present, and the samples must be pretreated. The Raman spectroscopy measurement methods were fast and simple, so the Raman spectroscopy methods for the drug analysis were explored in this paper. An optical fiber nano‐probe coated with gold nanoparticles was fabricated and used with surface‐enhanced Raman spectroscopy (SERS) to measure levofloxacin lactate. The resulting SERS spectra of levofloxacin lactate in mouse blood that was detected by the optical fiber nano‐probe clearly showed the characteristic wave numbers of levofloxacin lactate, indicating that optical fiber nano‐probes can be used with spectral techniques to analyze drugs in vitro or potentially even in vivo. Copyright © 2015 John Wiley &amp; Sons, Ltd.

Levofloxacin lactate and ceftizoxime for anti-infective therapy of acute edematous biliary pancreatitis: a comparison of efficacy

Objective To compare the anti-infective effects of levofloxacin lactate and ceftizoxime in treatment of acute edema biliary pancreatitis,so as to provide reference for clinical anti-infective therapy of acute pancreatitis.Methods A total of180 consecutive patients with acute edema biliary pancreatitis,who were treated in the emergency department of Changhai Hospital from January 2014 to November 2014,were selected in the present study,and they were randomly divided into levofloxacin lactate treatment group and ceftizoxime treatment group.In addition to conventional treatment including water fasting,gastrointestinal decompression,acid suppression,enzyme suppression and nutrition support,etc.,levofloxacin lactate therapy and ceftizoxime were given separately to control the infection in the two groups.Then the following parameters were observed:remission of vomiting,abdominal pain and bloating,open eating time,hospital stays,blood routine and biochemistry test,C reaction protein(CRP),serum amylase,and the absorption of inflammation of the pancreas in imaging.Results The levofloxacin lactate treatment group had a significantly higher effective rate than the ceftizoxime treatment group(P0.05).Compared with the ceftizoxime treatment group,the remission of vomiting and abdominal pain and bloating was significantly faster in the levofloxacin lactate treatment group(P0.01);the open eating time,hospital stays hemogram and blood amylase recovery were also significantly improved in the levofloxacin lactate treatment group(P 0.05);and the absorption of inflammation of the pancreas in CT imaging was significantly faster(P0.05).Conclusion The study shows that levofloxacin lactate is more effective than ceftizoxime in the anti-infective therapy of acute edema biliary pancreatitis.

Influences of levofloxacin salts on the metabolism of Escherichia coli by microcalorimetry

A microcalorimetric technique was used to evaluate the influence of both Levofloxacin lactate in sodium chloride injection (drug A) and Levofloxacin hydrochloride in sodium chloride injection (drug B) on the metabolism of Escherichia coli. By means of an isothermal calorimeter and ampoule method at 37 °C, the power-time curves of E. coli growth were obtained under different conditions. The parameters such as the growth rate constant k, maximum power output Pm, time tm corresponding to the maximum power output and inhibitory ratio I of these two drugs were obtained. The results reveal that the inhibitory abilities enhance with increasing concentrations of the two drugs. The critical growth concentration and the half-inhibitory concentration IC50 were 0.15 and 0.079 μg mL−1 (for drug A), 0.13 and 0.061 μg mL−1 (for drug B), respectively. These results show the drug A has slightly better inhibitory effect on E. coli than that of drug B.

Synthesis of Fe&lt;sub&gt;3&lt;/sub&gt;O&lt;sub&gt;4&lt;/sub&gt; Nanocrystals and Application in Photocatalytic Degradation of Levofloxacin Lactate

Fe3O4nanocrystals were synthesized with polyethylene glycol (PEG) and polyvinyl pyrrolidone (PVP) as dispersant respectively by solvothermal process. The nanocrystals were characterized in order to elucidate the morphology and structure by transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) respectively. The results indicated that Fe3O4 nanocrystals were cubic phases, and the morphologies of samples were globular nanoparticles structure no matter what dispersant was. The photocatalytic degradation of levofloxacin lactate (LVFX) revealed that Fe3O4nanocrystals promoted the degradation of LVFX. As the UV radiation time was only 30 min, the photocatalytic degradation percentage of LVFX with Fe3O4(magnetite) as catalyst was about 92%. However, the direct photolysis degradation percentage of LVFX without Fe3O4is about 85%.

Synthesis of conjugates of β-cyclodextrin with polyamidoamine dendrimers and their molecular inclusion interaction with levofloxacin lactate

Polyamidoamine (PAMAM) dendrimers of different generations (G2 and G4) conjugated with β-cyclodextrin (β-CD), PAMAM (G2, G4)-CD, were synthesized using substitution reaction from mono-6-iodine-β-cyclodextrin and PAMAM dendrimers. The resulting molecular structures were characterized by NMR, IR. The molecular interaction between various dendrimers and levofloxacin lactate (LFL) were investigated by monitoring the fluorescence of LFL in the presence of dendrimers in buffer solution (pH 7.4) at 25 °C. It was found that the PAMAM (G2, G4)-CD possesses higher sensitizing ability than that of the corresponding parent dendrimers and natural β-CD, and increases concomitantly with the increases of generation and content of β-CD, suggesting that the PAMAM (G2, G4)-CD possesses stronger inclusion ability with LFL. The possible interaction mechanism between PAMAM-CD and LFL was proposed by 1H NMR analysis and theoretical calculation. The results show that the LFL molecule is located at the amine end of dendrimer molecule and along the side of cyclodextrin cavities to form supramolecular complexes. Furthermore, results indicate that the main driving force of the complex could be attributed to the electrostatic interactions and hydrogen bonding between LFL and PAMAM-CD, as well as the synergistic effect of intermolecular forces.

Appropriate Dose for Degradation of Levofloxacin Lactate: Gamma Radiolysis and Assessment of Degradation Product Activity and Cytotoxicity

Antibiotic residues have the risk of promoting the emergence of resistant bacteria in the aquatic system. Gamma irradiation has been proven to be an effective technique to remove the contamination. However, degradation products might also promote antimicrobial resistance, especially when the active part of the parent structure remains unaltered. Therefore, degradation products have been analyzed in detail to evaluate their potential environmental effect. Levofloxacin lactate was employed as the targeted antibiotic to evaluate gamma radiolysis of antibiotics; the activity and cytotoxicity of the products were also analyzed. Levofloxacin lactate (10 μg mL−1) was removed >99% with the dose of 1 kGy. The G-values ranged from 1.643 to 0.193 with increasing radiation dose. Five main radiolysis products (mass/charge ratio [m/z] 346, 330, 318, 302, and 274) were identified by liquid chromatography-mass spectrometry (LC-MS). Results indicated degradation at the piperazinyl substituent and the quinolones moiety. The inhibitory concentration was about 0.2 μg mL−1 with 0.5 kGy, whereas the minimal inhibitory concentration (MIC) was 4 μg mL−1 and the byproduct (m/z 346) was found to have anti–Escherichia coli activity. Relative proliferation rates for lymphocyte under different doses were all >80, indicating that the irradiated levofloxacin lactate was nontoxic. All results revealed that 3 kGy was the appropriate dose for the radiolysis of levofloxacin lactate (10 μg mL−1).

The expressions of TF mRNA and TFPI mRNA of liver in rats with Vibrio vulnificus sepsis and the intervention effects of antibacterial agents

Objective To investigate the expressions of TF mRNA and TFPI mRNA of liver in rats with Vibrio vulnificus sepsis and to assess the interventional effects of cefoperazone sodium along with levofloxacin lac-tate. Method One hundred and ten male SD rats were divided (random number) into normal control group (NC group, n = 10), Vibrio vulnificus sepsis group (VV group, five subgroups n = 10 in each), drug intervention model (AA group, five subgroups n = 10 in each). The Vibrio vulnificus sepsis models and drug intervention models of rat were made. The reverse transcription polymerase chain reaction (RT-PCR) assay was employed for the measurement of TF mRNA and TFPI mRNA. ANOVA and t-test performed with SPSS version 12.0 software. Results Compared with NC, the expressions of TF mRNA in liver increased markedly 2 h,6 h, 12 h and 16 h af-termodeling in VV groups (P 0.05). Compared with VV groups, the expressions of TF mRNA in liver of rats in AA groups were greatly lowered 9 hours after administration of bactericide (P<0.05), and the expressions of TFPI mRNA in liver of rats in AA groups were significantly higher 12h and 16 h after intervention (P<0.05). Conclusions There is a obvious imbalance between coagulation and anticoagulation functions of circulation system during Vibrio vulnificus sepsis, and the imbalance can be corrected gradually after treatment with antibacterial agents. Key words: Vibrio vulnificus; Sepsis; Tissue factor (TF) ; Tissue factor pathway inhibitor (TFPI); Antibacterial agents

SYNTHESIS OF POLYAMIDOAMINE DENDRIMERS MODIFIED BY CYCLODEXTRIN AND THEIR MOLECULAR INCLUSION INTERACTION WITH LEVOFLOXACIN LACTATE

The polyamidoamine (PAMAM)dendrimers having different generations (G2 and G4)modified by β-cyclodextrin (β-CD),PAMAM( G2,G4)-β-CD,were synthesized using substitution reaction from mono-6- iodine-β-cyclodextrin and PAMAM dendrimers,and the molecular structures were characterized by 1H-NMR and IR.The molecular interaction between various dendrimers and levofloxacin lactate ( LFL) was determined by fluorimetry in buffer solution (pH = 7.4)at 25℃ .It was found that the PAMAM(G2,G4)-β-CD possesses higher sensitizing ability than that of the corresponding parent dendrimers and natural β-CD,and it increases concomitantly with the increases of generation and content of β-CD,suggesting that the PAMAM( G2,G4)-β- CD possesses strong inclusion ability with LFL.The possible interaction mechanism between PAMAM-CD and LFL was proposed by 1H-NMR analysis and theoretical calculation.The results showed that the LFL molecule was placed in the amino end of dendrimer molecule and between the sides of cyclodextrin cavities to form supramolecular complexes.Furthermore,the results indicate that the main driving force of the system could be attributed to the electrostatic interaction as well as hydrogen bonding between the LFL and PAMAM-CD,and fully reflects the synergistic effect of intermolecular forces.

SYNTHESIS OF POLYAMIDOAMINE DENDRIMERS MODIFIED BY CYCLODEXTRIN AND THEIR MOLECULAR INCLUSION INTERACTION WITH LEVOFLOXACIN LACTATE

The polyamidoamine (PAMAM)dendrimers having different generations (G2 and G4)modified by β-cyclodextrin (β-CD),PAMAM( G2,G4)-β-CD,were synthesized using substitution reaction from mono-6- iodine-β-cyclodextrin and PAMAM dendrimers,and the molecular structures were characterized by 1H-NMR and IR.The molecular interaction between various dendrimers and levofloxacin lactate ( LFL) was determined by fluorimetry in buffer solution (pH = 7.4)at 25℃ .It was found that the PAMAM(G2,G4)-β-CD possesses higher sensitizing ability than that of the corresponding parent dendrimers and natural β-CD,and it increases concomitantly with the increases of generation and content of β-CD,suggesting that the PAMAM( G2,G4)-β- CD possesses strong inclusion ability with LFL.The possible interaction mechanism between PAMAM-CD and LFL was proposed by 1H-NMR analysis and theoretical calculation.The results showed that the LFL molecule was placed in the amino end of dendrimer molecule and between the sides of cyclodextrin cavities to form supramolecular complexes.Furthermore,the results indicate that the main driving force of the system could be attributed to the electrostatic interaction as well as hydrogen bonding between the LFL and PAMAM-CD,and fully reflects the synergistic effect of intermolecular forces.

Novel supramolecular system of amphiphilic hyperbranched polymer with β-cyclodextrin and hyperbranched topography cavities: Synthesis and selective encapsulation

Novel supramolecular system of amphiphilic hyperbranched polymer with hyperbranched poly(β-cyclodextrin) core was designed and synthesized to accomplish a so-called selective encapsulation, where two types of guest molecules can be encapsulated into two types of molecular cavities from β-cyclodextrin (β-CD) and topography structure of hyperbranched polymer, respectively. The double molecular recognition behaviors from β-CD and hyperbranched cavities drive one guest to go into the former, the other guest to the latter. This selective encapsulation was further confirmed via the release profiles and sequences of Levofloxacin lactate (LL) and Phenolphthalein (PP). LL presents a sustained release period followed by an almost non-release stage, while PP releases on a quite slow rate at first, subsequently on the linearly increasing rate. At the early stage, the release of LL dominates in comparison with PP, and then the release rate of PP increases to play a determinate role in the release system. It can be attributed to the existence of two guests in the different molecular cavities with the different microenvironments. The observed selective encapsulation of supramolecular system is a new phenomenon, which is helpful to extend the application of CD-based hyperbranched polymers in supramolecular science and complex drug delivery system.

The Action of a Novel Fluoroquinolone Antibiotic Agent Antofloxacin Hydrochloride on Human-Ether-à-go-go-Related Gene Potassium Channel

The administration of certain fluoroquinolone antibacterials has recently been linked to QT interval prolongation, raising the clinical concerns over the cardiotoxicity of these agents. In this study, the effects of a novel fluoroquinolone, antofloxacin hydrochloride (AX) on human-ether-à-go-go-related gene (HERG) encoding potassium channels and the biophysical mechanisms of drug action were performed with whole-cell patch-clamp technique in transiently transfected HEK293 cells. The administration of AX caused voltage- and time-dependent inhibition of HERG K+ current (I(HERG/MiRP1)) in a concentration-dependent manner but did not markedly modify the properties of channel kinetics, including activation, inactivation, deactivation and recovery from inactivation as well. In comparison with sparfloxacin (SPX), levofloxacin lactate (LVFX), the potency of AX to inhibit HERG tail currents was the least one, with an IC(50) value of 460.37 microM. By contrast, SPX was the most potent compound, displaying an IC(50) value of 2.69 microM whereas LVFX showed modest potency, with an IC(50) value of 43.86 microM, respectively. Taken together, our data suggest that AX only causes a minor reduction of I(HERG/MiRP1) at the estimated free plasma level.

Dynamic expressions of liver tissue apoptosis-related genes of Vibrio vulnificus sepsis rats and the effects of antibacterial agents

Dynamic changes in mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax and apoptosis-inhibiting gene Bcl-2 of vibrio vulnificus sepsis rats were detected and the effects of antibacterial agents were examined. The rat model with Vibrio vulnificus sepsis (VV group) was established and some of the Vibrio vulnificus sepsis rats were treated with antibacterial agents (AA group). The mRNA expressions of Fas, Bax and Bcl-2 were measured by reverse transcription polymerase chain reaction (RT-PCR). As compared with normal control group (NC group), the expressions of Fas and Bax mRNA in liver tissue at all different time points in VV group were increased significantly (P<0.05), and the highest levels of Fas and Bax mRNA expressions were 6 and 12 h after the infection, respectively. At the same time, the expression of Bcl-2 mRNA in liver tissue at all different time points in VV group were decreased significantly (P<0.05), and the lowest level of Bcl-2 mRNA expression appeared 2 h after the infection. The mRNA expressions of Bcl-2 in liver tissue 9 and 12 h after the infection in AA group were increased significantly (P<0.05) compared with NC group, while the expressions of Fas and Bax mRNA were not significantly different from those of NC group. Compared with VV group, the expression of Fas mRNA in AA group was decreased (P<0.05) and Bax mRNA was decreased significantly 12 and 16 h after the infection (P<0.05), while the expressions of Bcl-2 mRNA were increased significantly 9, 12 and 16 h after the infection (P<0.05). It is concluded that the mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax were increased remarkably in vibrio vulnificus sepsis rats, whereas the expression of apoptosis-inhibiting gene Bcl-2 mRNA was decreased obviously in sepsis rats in early stage. The treatment with cefoperazone sodium and levofloxacin lactate could inhibit the expression of Fas mRNA and Bax mRNA and enhance the expression of Bcl-2 mRNA at the same time.