Influences of levofloxacin salts on the metabolism of Escherichia coli by microcalorimetry

Abstract

A microcalorimetric technique was used to evaluate the influence of both Levofloxacin lactate in sodium chloride injection (drug A) and Levofloxacin hydrochloride in sodium chloride injection (drug B) on the metabolism of Escherichia coli. By means of an isothermal calorimeter and ampoule method at 37 °C, the power-time curves of E. coli growth were obtained under different conditions. The parameters such as the growth rate constant k, maximum power output Pm, time tm corresponding to the maximum power output and inhibitory ratio I of these two drugs were obtained. The results reveal that the inhibitory abilities enhance with increasing concentrations of the two drugs. The critical growth concentration and the half-inhibitory concentration IC50 were 0.15 and 0.079 μg mL−1 (for drug A), 0.13 and 0.061 μg mL−1 (for drug B), respectively. These results show the drug A has slightly better inhibitory effect on E. coli than that of drug B.