Zinc Levels Research Articles

196 Effects of zinc source and level in low ABC-4 diets on nursery pig growth performance and fecal dry matter

Abstract Incorporating pharmacological levels of Zn in nursery pig diets is a well-recognized practice for reducing post-weaning diarrhea, enhancing growth performance, and enhancing gut health. We hypnotized that the use of a specific form of ZnO (small aggregates and agglomerated particles; SAAP), which provides a greater antibacterial property than standard ZnO, could be added at reduced levels without compromising piglet performance. This study aimed to explore the effects of different levels of SAAP Zn source (HiZox, Animine, France) in low ABC-4 diets with low or high CP content. A total of 360 weanling barrows [DNA 200 × 400, initial body weight (BW) = 5.9 ± 0.4 kg] were used in a 38-d experiment. Pigs were randomly assigned to pens (5 pigs/pen) and pens assigned to 1 of 6 treatments with 12 pens/treatment. The experiment consisted of phase 1 from d 0 to 10, phase 2 from d 11 to 24, and phase 3 from d 25 to 38. Treatments consisted in a negative control (NC), which had 150 ppm of Zn (HiZox) throughout the experiment and the remaining treatments were: positive control (PC) containing 3,000 ppm (phase 1) and 2,000 ppm (phase 2) of Zn provided by ZnO; low-HiZox containing 500 ppm (phase 1) and 300 ppm (phase 2) of Zn; low-HiZox-low-CP was similar to low-HiZox but contained less CP (19.3%) than the NC, PC, low and high-HiZox diets (21.3%); high-HiZox containing 800 ppm (phase 1) and 500 ppm (phase 2) of Zn; and high-HiZox-low-CP was similar to high-HiZox but contained 19.3% CP. In phase 3, all pigs were fed a common diet containing 150 ppm of Zn (HiZox) and high CP. For the experimental period (d 0 to 24), average daily gain (ADG; quadratic, P = 0.007) and average daily feed intake (ADFI; quadratic, P = 0.018) increased as HiZox increased (Table). Pigs fed high-HiZox-low-CP had poorer gain to feed ratio (G:F; P < 0.05) than NC, PC, low-HiZox, and high-HiZox. Additionally, in both experimental and overall periods, pigs fed diets with low CP had decreased G:F (P = 0.043) than those fed the same Zn levels but with high CP. Overall, pigs fed high-HiZox-low-CP had decreased G:F than pigs fed high-HiZox only (732 vs. 761 g/kg, respectively; P < 0.05), with other treatments intermediate. For fecal dry matter (DM), pigs fed low CP diets had greater (P = 0.008) DM compared with pigs fed high CP. Thus, the inclusion of HiZox at 800 and 500 ppm in phases 1 and 2, respectively, in a low-ABC-4 diet might be a strategy to reduce the use of pharmacological levels of Zn from ZnO in nursery diets.

202 Source matters: long-term effects of nursery sanitary condition and dietary Zinc source and level on growth performance, diarrhea incidence, and carcass metrics of pigs

Abstract There is growing evidence that requirements for particular nutrients increase when pigs are kept under low sanitary conditions (LSC). It may be hypothesized that dietary zinc (Zn) source can exert influence on immune status, which may influence resistance to subclinical and clinical diseases. The objective was to investigate the long-term effects of nursery sanitary condition and dietary Zn source and growth performance, diarrhea incidence, and carcass metrics of pigs. An experiment was performed with 480 mixed-sex weanling pigs (initial body weight = 6.4 ± 0.38 kg) which were assigned to 1 of 5 treatments during the nursery (42 d) as follows: 1) pigs housed in high sanitary conditions (HSC) and fed 50 mg/kg Zn from Zn-amino acid complex (ZnAA), pigs housed in LSC and fed either 2) 50 mg/kg Zn from ZnAA, 3) 100 mg/kg Zn from ZnAA, 4) 100 mg/kg Zn and 0.4 mg/kg chromium (Cr) from Zn+Cr-amino acid complex (Zn+CrAA), or 5) 100 mg/kg Zn from inorganic Zn (ZnSO4). Pigs were then placed onto a common grower (42 d) and finisher (42 d) diets and housed under similar, standard conditions and were monitored until slaughter. Growth performance, diarrhea incidence, and carcass characteristics were assessed. Pigs housed in LSC and fed 100 mg/kg from ZnSO4 had less average daily feed intake (ADFI) during wk 1 and 2 of nursery (0.29 vs. 0.33 ± 0.01 kg/d; P < 0.05) and poorer feed to gain (FGR) during wk 3 and 4 of nursery (1.76 vs. 1.54 ± 0.03 kg/kg; P < 0.05), compared with the other groups. Overall nursery FGR was poorer in pigs housed in LSC and fed 100 mg/kg from ZnSO4 compared with the other groups housed in LSC (1.63 vs. 1.54 ± 0.02 kg/kg; P < 0.05). Diarrhea incidence tended to increase in all groups housed in LSC compared with the group housed in HSC during week 1 of nursery (91.1 vs. 80.4 ± 3.11%; P < 0.10), while no effect on diarrhea incidence was observed during the subsequent week (P > 0.10). Slaughter weight was increased in pigs housed in LSC and fed 100 mg/kg Zn and 0.4 mg/kg Cr from Zn+CrAA compared with pigs housed in LSC and fed 100 mg/kg from ZnSO4 (123.4 vs. 120.1 ± 0.63 kg; P < 0.05). Housing pigs in LSC during the nursery seems to trigger negative effects on growth performance and diarrhea incidence during nursery only, but these effects do not seem to be translated into the grower and finisher phases. Feeding ZnAA and Zn+CrAA aid the pigs in responding to LSC during nursery and may be an approach to help minimize negative effects of immune system stimulation.

203 The relationship between potentiated zinc oxide and monovalent copper in a dietary administration for weanling piglets

Abstract The aim of this study was to evaluate different dietary level of Zinc (Zn) and Copper (Cu) supplementation according to European and non-European levels on the health and performance of pigs 14 d after weaning. A total of 120 piglets [initial body weight = 7.14 ± 0.92 kg] were divided into 4 experimental treatments: positive control (PC, 2,500 ppm of Zn, ZnO) and 3 treatments in which Zn and Cu were added through potentiated ZnO and Cu2O according to different European and non-European levels of inclusion: EU (120 ppm of Zn; 140 ppm of Cu), non-EU+ (300 ppm of Zn; 200 ppm of Cu) and non-EU- (300 ppm of Zn; 140 ppm of Cu). Growth performance, serum Zn and Cu concentrations, fecal score, blood biomarkers of intestinal integrity, and fecal microbial composition were examined and analyzed using GLM and MIXED procedures of SAS. Fecal samples at 14 d were collected and the V3–V4 hypervariable regions of the bacterial 16S gene were sequenced in one Illumina MiSeq run. Body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (FCR) and (feed efficiency (FE) were not affected by the experimental treatments. The fecal score suggests that during the first 14d, the consistency of the feces tended (P < 0.10) to be less in the three treatment groups while in PC, the greatest concentration of Zn favored a better fecal consistency. The initial serum Zn and Cu concentrations were similar for all dietary treatment groups. After 14 d, serum Zn concentration was significantly greater in PC (15.71 ± 6.25 µg/L, P < 0.05). No differences were highlighted in serum Zn concentration among the other 3 treatment groups. Serum Cu concentrations revealed statistically significant results (P < 0.05) on 14 d after weaning in EU, non-EU+ and non-EU- groups, showing greater concentrations of Cu (17.65 ± 5.49 µmol/L, 17.73± 3.46 µmol/L 18.33 ± 4.13 µmol/L, respectively) compared with PC (11.87 ± 3.60 µmol/L). These results suggested that the EU level of inclusion of both potentiated Zn and monovalent Cu is enough to reach the same Cu and Zn plasma concentrations of non-EU treatments. Plasma DAO was negatively (P < 0.05) affected in non-EU-. Firmicutes and Bacteroidetes were the most abundant phyla in fecal microbiome. Non-EU- contributed to a significant decrease in biodiversity in fecal microbiota as depicted with Shannon for diversity and Simpson for evenness indexes (P < 0.05). In fecal samples, the presence of genera linked to a greater disruption of the gut barrier (Escherichia-Shigella) was depicted in non-EU- group, indicating significant modifications of the microbial community. These results suggest the need for a balanced supplementation of Cu and Zn through more bioavailable sources. The use of potentiated ZnO and Cu2O according to European levels could represent a valid strategy to enhance weanling piglets gut health and to reduce the environmental impact.

Evaluation of Serum Level of Zinc, Copper and Magnesium in Asthmatic Children and Their Correlation to Disease Severity

Evaluation of Serum Level of Zinc, Copper and Magnesium in Asthmatic Children and Their Correlation to Disease Severity

Influence of Dietary Zinc Supplementation on Serum Minerals (Zinc, Calcium, Phosphorus) and Liver Enzyme Level (ALP, ALT, GGT) in Layer Chicken

The Indian industry is presently focussed in production of eggs that have higher or enriched levels of certain nutrients. These eggs are capable of safeguarding the health of consumers. Designer eggs have high market demand because of the consumers’ willingness to purchase designer eggs due to its’ nutritional qualities additional to regular eggs. Considering these facts the current study was planned to produce zinc enriched eggs by supplementing laying hens’ diet with required levels of zinc. The present study was conducted at the Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Mannuthy, for 12 weeks. A total of 28 week old thirty two laying hens, belonging to crossbred (White Leghorn N strain and Desi) housed in the animal house attached to the department were used in the experiment. The treatments of this study included a control diet (according to BIS, 2007) fed group and zinc-supplemented group. Each of the experimental treatments had four replicates with four birds with all standard managemental conditions. The functional liver status of birds was not altered due to zinc supplementation, since the present study neither caused any toxicity nor imparted any negative effect on the production performance. Highly significant effect was observed on serum concentration of zinc and calcium. However, the phosphorus levels of treated birds were not significantly different from the control group.

99 Phytochemicals as medical growth promoter replacement on growth performance in wean to finisher pigs

Abstract Stress can disturb the ecological balance of the microbiota residing in the GI tract of piglets leading to dysbiosis. This unhealthy imbalance causes the piglets to be more vulnerable to deleterious effects induced by opportunistic pathogens. Antibiotics and increased zinc levels have often been used as dietary supplements to help minimize these stressors in pigs. Emerged evidence suggests that phytochemicals can promote growth by boosting nutrient digestibility, immune cell activity, and possessing bactericidal characteristics. We evaluated two phytochemicals formulations as safer and more environmentally friendly dietary alternatives for postweaning pigs. We used a total of 240 weaned piglets [average body weight (BW): 6.26 ± 0.36 kg] which were blocked (n = 8) by BW and then randomly assigned to 1 of 5 dietary treatments. Treatments were negative control (NC), Mecadox 10 (Phibro Animal Health Corp., Teaneck, NJ) + ZnO positive control (PC), Mecadox + phytochemical 1 (Lippia origanoides; Phyt1+AB), phytochemical 1 alone (Phyt1), and phytochemical 2 (Lippia origanoides + Rosmarinus officinalis + Sodium humates; Phyt2). The pigs were fed a 7-phase feeding regime: Nursery phase (NP) 1, 2, and 3, Grower phase 1 and 2 (Grower), and Finisher phase 1 and 2 (Finisher). The antibiotic and ZnO were withdrawn at the end of NP3, while Phyt1 and Phyt2 remained in feed throughout the entire study period. Data were analyzed using Mixed procedure of SAS (Cary, NC) with treatments as the main effect and BW blocks as the random effect. Pen was the experimental unit. Supplemental phytochemicals (Phyt1+AB, Phyt1, Phyt2) had greater average daily gain (ADG) and gain to feed ratio (G:F) during NP1 when compared with the pigs fed NC, but were still less than pigs fed PC (P < 0.0001). Pigs fed PC maintained a greater ADG during nursery overall than NC, Phyt1, and Phyt2 with pigs fed Phyt1+AB as intermediate (P < 0.0001). Phyt1 exerted an adverse effect (Phyt1+AB and Phyt1) on ADG during relatively mild stress in Grower phase (Grower, P = 0.0146), and resulted in the lighter BW at the end of Grower than pigs fed PC (P = 0.031). Notably, the pigs experienced summer heat stress during the Finisher phase, of which, the presence of phytochemicals minimized. Pigs fed Phyt2 had a greater ADG during Finisher than pigs fed NC with pigs fed PC, Phyt1+AB, and Phyt1 as intermediates (Finisher, P = 0.0419), while pigs fed Phyt1+AB and Phyt2 had greater G:F when compared with pigs fed PC and NC with pigs fed Phyt1 as intermediate (Finisher, P = 0.0032). Collectively, we demonstrated that phytochemical 2 can be a safer medication diet replacement to help pigs sustain weaning and heat stress. The mode of action and the optimum concentrations of these phytochemicals to achieve maximum benefits need further investigation.

Assessment of Macro and Micro Mineral Compositions at Different Depths of Forest Nursery Soils in Federal University of Agriculture Abeokuta, Nigeria

Forest soil is characteristically fundamental in the establishment of healthy seedlings for eventual planting out on the field. It is composed of mineral particles, organic matter, water, air, and numerous microorganisms. Two forest nursery soils at different depths were investigated at the Federal University of Agriculture Abeokuta (FUNAAB) for their mineral compositions. With the aid of a soil auger, twenty soil samples were randomly collected from different points in two sites at different depths (0-15 cm and 15-30 cm, air dried and taken to the laboratory for further analysis. Site 1 and site 2 (frequently cultivated portion for agroforestry bare-root seedlings and intermittently used portion). The obtained data were subjected to descriptive statistics. The composition of mineral elements varied with sites and depths. The level of Potassium was higher at depths 0 – 15 cm and 15 – 30 cm in site 2 than other macro-minerals (Sodium, Calcium and Magnesium) when compared between sites. The order of concentration includes K>Mg>Na>Ca. Micro-minerals were significantly higher at soil depth 0 – 15 cm in site 2 than site 1 (Fe>Zn>Mn>Cu). At soil depth 15 – 30 cm, levels of copper, zinc and manganese were significantly higher (p < 0.05) in site 1 with the following trend Cu>Fe>Zn>Mn. The study showed that forest nursery soils varied in their mineral composition along with depth in the studied sites. Site 2 had better physicochemical properties and higher level of some essential minerals needed for good performance of seedling growth at nursery stage.

Association between biomarkers of zinc and copper status and heart failure: a meta-analysis.

Previous studies have investigated the relationship between heart failure (HF) and levels of zinc and copper, but conflicting results have been reported. This meta-analysis aims to clarify the role of zinc and copper in HF progression by examining the associations between HF and concentrations of these minerals. We utilized STATA 12.0 software to calculate the standard mean difference (SMD) and 95% confidence interval (CI) for serum zinc and copper levels in patients with HF compared with healthy controls (HCs). The meta-analysis indicated a lower serum zinc level in patients with HF compared with HCs, using a random effects model (SMD=-0.77; 95% CI: -1.01, -0.54; I2=61.9%, the P-value for Q test=0.002). Additionally, the meta-analysis showed an increased serum copper level in patients with HF compared with HCs, using a random effects model (SMD=0.66; 95% CI: 0.09, 1.23; I2=93.8%, the P-value for Q test<0.001). Meta-regression analysis indicated that publication year, age, and gender were not responsible for heterogeneity across studies. This meta-analysis demonstrates that patients with HF have lower serum zinc and higher copper concentrations compared with healthy subjects. However, the potential of zinc supplementation as a therapy for HF should be approached with caution. The heterogeneity among the included studies was found to be high. It is recommended that further well-designed large sample studies be conducted to validate these findings.

Serum zinc levels and body composition variability as trajectory for hyperlipidemic and dyslipidemic effect among welders exposed to welding fumes and smoking: A biomarker for cardiovascular health

Welding is a common method for joining metals by heating them to the welding temperature. Exposure to welding fumes has a serious effect on the health of welders. This study examined serum zinc variability and body composition as route for hyperlipidemia and dyslipidemia in welders exposed to welding fumes and smoking, exploring the possibilities for the risk of possible cardiovascular disease. The experimental case control design was adopted in the study. Forty apparently healthy adult males were randomly selected comprising of twenty control group (non-smokers and smokers without welding experience) and twenty experimental group (non-smokers and smokers with welding experience) welders. Data obtained were represented as Mean ± SEM while comparison of means across group was done by one-way ANOVA followed by Tukey's multiple comparison for post hoc test at p-value < 0.05 level of significance using Graph Pad prism version 8. The data obtained showed that the body mass index (BMI) of smokers (non-welders and welders) were slightly reduced while that of non-smoking welders was increased compared to the control. The serum zinc level increased among the smoking welders, while the smoking non-welders and non-smoking welders decreased when compared to the control group (p < 0.05). Exposure to welding fumes has been shown to increase total cholesterol levels compared to the control. Weld fumes significantly (p < 0.05) increased high-density lipoproteins (HDL) levels among smoking non-welders compared to the control group, while, HDL was reduced in non-smoking welders and smoking welders, respectively (p < 0.05). Triglyceride levels significantly (p < 0.05) increased in all experimental groups compared to control levels (p < 0.05). Exposure to welding fumes and smoking caused significant changes in serum zinc, HDL and triglycerides levels with implications for the formation of plaques around the arteries interfering with the effective flow of blood through the vascular system, with implications of hyperlipidemia and dyslipidemia. This study recommends that further studies should be done using biomarkers from urine or toe nails.

Effect of phosphorus and zinc levels on growth and yield of greengram (Phaseolus radiata L.)

Effect of phosphorus and zinc levels on growth and yield of greengram (Phaseolus radiata L.)

Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion

Background: According to the World Health Organization, zinc deficiency is a growing global health problem, impacting 31% of the world’s population. Zinc deficiency is a common comorbidity with many chronic diseases, from skin to kidney diseases. Given the increasing interest in zinc’s role in disease onset and progression, there is a dire need for reproducible approaches to manipulate zinc homeostasis. Here, we provide proven strategies for inducing and validating zinc deficiency and zinc repletion in a mouse model. Experimental Design: After acclimating to a ZnA (zinc adequate) diet, mice are randomly assigned to either a ZnA or ZnD (zinc deficient) diet. To replete zinc levels (ZnR), a subset of ZnD mice is returned to the ZnA diet. Animal growth rates and plasma zinc levels are assessed to validate zinc status. Results: Mice placed on the ZnD-diet have reduced body weights (26.48g ± 0.78) compared to mice maintained on the ZnA-diet (28.12g ± 0.13). However, zinc repletion promotes weight gain (27.87g ± 1.01) resulting in body weights comparable to mice maintained on the ZnA-diet. Furthermore, mice maintained on the ZnA-diet have plasma zinc levels of 3.19 μM/g Body Weight. While plasma zinc levels are 40% lower in mice place on the ZnD-diet (1.29 μM/g Body Weight). In contrast, plasma zinc levels (2.44 μM/g Body Weight) are increased in ZnR mice. Conclusion: These findings validate that the described dietary approach is an effective strategy for inducing zinc deficiency and zinc repletion. Significance: This cost-effcient approach will enhance the scientific rigor and reproducibility of studies examining the impact of zinc deficiency, and repletion on disease onset and progression. Funding Source: R21 DK119879, R01 DK-133698. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Influence of different level of sulphur and zinc on physico-chemical properties of soil yield attributes and nutrient content of maize (Zea mays L.) Shakti 8484

Influence of different level of sulphur and zinc on physico-chemical properties of soil yield attributes and nutrient content of maize (Zea mays L.) Shakti 8484

A comparative study of acquisition of heavy metals and its toxicological effects in fish species: Siluriformes and Channa punctata

The heavy metals damage fish's physiologic and chemical composition, causing structural damage and hindering their activities. Lucknow's fisheries sector, accounting for 1% of GDP, faces contamination from heavy metals, pesticides, and fertilizers. This study examines heavy metal levels in Siluriformes and Channa punctata fish and their buildup in the Gomati River water reservoir. Fish samples from the Gomati River near Lucknow, India, were collected and dissected into pieces with stainless steel blades. The samples were then dried at 100°C for 24 hours, revealing the anatomical features of Siluriformes and Channa punctata species. The Pb, a heavy metal, was found to have the highest concentration in the scales of Siluriformes, with values in various regions, while Cd had the lowest concentration, ranging from 0.016 to 0.112 mg/kg. Excessive consumption of fish can be dangerous due to toxic metallic elements accumulation. Siluriformes and Channa punctata fish species have high levels of Lead and Cadmium, respectively, indicating potential health risks. Channa punctata and Siluriformes fish have higher heavy metal concentrations than Siluriformes, with lead, iron, and nickel levels beyond WHO limits, while chromium, zinc, cadmium, and copper levels are below WHO limits.

The association of circulating levels of Zinc and Iron in adults patients with prediabetes and type two diabetes

The association of circulating levels of Zinc and Iron in adults patients with prediabetes and type two diabetes

ARTERIAL STIFFNESS ASSOCIATES WITH MALNUTRITION IN HYPERTENSIVE PATIENTS WITH CONCOMITANT CHRONIC PANCREATITIS

Objective: The association between pancreatitis and cardiovascular diseases remains incompletely understood. Malnutrition in chronic pancreatitis may be associated with an increase in arterial stiffness in hypertension. Objective: to evaluate interaction between malnutrition markers and arterial stiffness in patients with hypertension and chronic pancreatitis. Design and method: Methods: 70 non-diabetic patients with 1st degree hypertension with concomitant chronic pancreatitis (median age – 52,4 [43,8;63,7] years) were included in the study. We divided all included patients into two groups: 1st group – 41 patients with malnutrition, 2nd group – 29 patients without malnutrition. Standard laboratory blood tests, haematological parameters, lipid profile, glucose, renal and liver function tests, pancreatic fecal elastase-1, total protein, serum albumin, serum iron, vitamin 25(OH)D, serum zinc and magnesium detection were performed for all patients. The groups were comparable in terms of age, gender structure, functional state of the kidneys. Non-parametric methods were used for statistical data analysis. To determine the prognostic significance, the ROC analysis was performed. Significant differences were considered when the p-value was p&lt;0.001 Results: In patients with a combined course of hypertension and chronic pancreatitis with established malnutrition, an increase in arterial stiffness was significantly more often compared to patients with normal nutritional status - 83% and 62%, respectively (p&lt;0.001). A significantly lower level of brachial artery augmentation index was established by 43% (p&lt;0.001) in the first group. It was established that patients with malnutrition had a significantly higher caritid-femoral pulse wave velocity by 17.5% (p&lt;0.001). Correlations were established between brachial artery augmentation index and serum levels of zinc (R=0.63, p&lt;0.001), magnesium (R=0.66, p&lt;0.001), albumin (R=0.58, p&lt;0.001), vitamin 25(OH)D (R=0.68, p&lt;0.001) in patients with the combined course of hypertension and chronic pancreatitis. ROC analysis revealed that serum zinc level had an excellent diagnostic power for arterial stiffness in hypertensive patients with chronic pancreatitis (AUC = 0.910). Serum albumin had very good diagnostic power for arterial stiffness in hypertensive patients with chronic pancreatitis (AUC = 0.859). Conclusions: Arterial stiffness may be associated with malnutrition in hypertensive patients with concomitant chronic pancreatitis.

Improving of test utilization through modification of the electronic ordering system for blood zinc level

Improving of test utilization through modification of the electronic ordering system for blood zinc level

Comparison of zinc levels in mothers with and without abortion: A systematic review and meta-analysiss

IntroductionZinc is an essential trace element involved in different physiological functions. During pregnancy, it plays a crucial role in healthy embryogenesis. Abortion is the most severe problem associated with early pregnancy complications. This study aims to compare the levels of Zn in mothers with and without abortion. MethodsThis study is a systematic review and meta-analysis of studies published between 1980 and 2022 in PubMed, Science Direct, Pro Quest, Wiley, Web of Science, and Scopus databases. The search was conducted using both main and Mesh keywords, specifically targeting terms related to abortion, pregnancy loss, and zinc. Heterogeneity in the studies included in the meta-analysis was evaluated using statistical tests such as the chi-square test, I2, and forest plots. Publication bias was assessed using Begg's and Egger's tests. All analyses were conducted using Stata 15, and statistical significance was considered at p < 0.05. ResultsIn general, nine studies were included in this analysis. Based on the results of the meta-analysis, the mean difference in the Zn level between mothers with and without abortion is equal to 193.18 (95 % CI; 107.11 to 279.25, P ≤ 0.001). In other words, the level of Zn in the group of mothers without abortion is 193 units higher than the group of mothers with abortion. The investigation into the comparison of Zn levels in mothers with and without abortion did not reveal any indication of publication bias according to both Begg's test (P-value = 0.858) and Egger's test (P-value = 0.270). Conclusionthe study findings indicate a significant association between lower levels of Zn in mothers with abortion compared to mothers without abortion. This suggests that a higher level of Zn may have a preventive effect on the occurrence of abortion in pregnant women. The data also highlights the positive role of trace metals, particularly Zn, in influencing pregnancy outcomes and suggests that maintaining adequate levels of Zn may reduce the likelihood of abortion occurrence, along with other contributing factors. It is important to note that further research, including prospective cohort and experimental studies, is needed to provide more substantial evidence and strengthen these findings.

Effect of nitrogen and zinc fertilizer on Zn biofortification in pearlmillet (Pennisetum glaucum)

A field experiment was conducted during the rainy (kharif) season of 2011–12 at Mirzapur, Uttar Pradesh to study the response of pearlmillet [Pennisetum glaucum (L.) R. Br.] to nitrogen and zinc on Zn biofortification in this crop. The field experiment was laid out in a factorial randomized block design, with 3 replications, comprising 4 nitrogen (N) levels (0, 20, 40 and 60 kg N/ha) and 3 levels of zinc (0, 5 and 10 kg Zn/ha) with constant rates of phosphorus and potassium at 40 and 30 kg/ha respectively. Application of 60 kg N/ha and 10 kg Zn/ha resulted in the maximum grain yield, N content and uptake by grain and stover of pearlmillet. Zinc content in grain linearly enhanced with sole application of N and Zn levels up to 20 kg N/ha and 5 kg Zn/ha. Combined application of 5 kg Zn/ ha × 20 kg N/ha was found optimum for enhancement of Zn content in grains of pearlmillet. Agronomic nitrogen and zinc efficiency decreased with the increasing nitrogen and zinc levels.

EVALUATION OF HEAVY METALS' CONCENTRATION AND PHYSICOCHEMICAL PARAMETERS OF GROUNDWATER WITHIN JOS METROPOLIS OF PLATEAU STATE, NIGERIA

This paper studied the quality of drinking water from borehole sources in Jos and its environs. The level of heavy metal concentrations and some physicochemical parameters were determined using standard methods. The result of heavy metal concentrations analyzed within the ten (10) sampling points shows that JS1 sampling point had a significantly high (p&lt;0.05) concentration of copper (0.0064 mg/L) and lowest in JS1 sampling point (0.001 mg/L). Chromium had a significantly high (p&lt;0.05) concentration in JS10 sampling point (0.718 mg/L) and lowest in JS1 (0.153 mg/L). Manganese had significantly high (p&lt;0.05) concentration in JS4 sampling point (0.198 mg/L) and lowest in (JS6 0.020 mg/L). Iron was significantly high (p&lt;0.05) in JS4 sampling point with the concentration of 1.388 mg/L and lowest value of 0.020 mg/L in JS5. Zinc had a significantly high (p&lt;0.05) in JS4 sampling point with the concentration of 0.085 mg/L. Lead had significantly high (p&lt;0.05) in JS8 sampling point with a concentration of 0.005 mg/L. Cadmium and nickel were not detected in all ten (10) sampling points. The electrical conductivity and pH and were found to be in the range of (0.000 - 350 µSv/cm) and (4.81 – 6.70), respectively. This research findings suggest that continued water quality monitoring should be carried out to check the concentration levels of copper, zinc, manganese, cadmium, nickel, lead and physicochemical parameters electrical conductivity and pH in those ten (10) sampling points to prevent them from been above the limit of World Health Organization (WHO) permissible limit and also creating...

Factors Influencing Wound Healing in Diabetic Foot Patients.

Background and objectives: Diabetic foot stands out as one of the most consequential and devastating complications of diabetes. Many factors, including VIPS (Vascular management, Infection management, Pressure relief, and Source of healing), influence the prognosis and treatment of diabetic foot patients. There are many studies on VIPS, but relatively few studies on "sources of healing". Nutrients that affect wound healing are known, but objective data in diabetic foot patients are insufficient. We hypothesized that "sources of healing" would have many effects on wound healing. The purpose of this study is to know the affecting factors related to the source of healing for diabetic foot patients. Materials and Methods: A retrospective review identified 46 consecutive patients who were admitted for diabetic foot management from July 2019 to April 2021 at our department. Several laboratory tests were performed for influencing factor evaluation. We checked serum levels of total protein, albumin, vitamin B, iron, zinc, magnesium, copper, Hb, HbA1c, HDL cholesterol, and LDL cholesterol. These values of diabetic foot patients were compared with normal values. Patients were divided into two groups based on wound healing rate, age, length of hospital stay, and sex, and the test values between the groups were compared. Results: Levels of albumin (37%) and Hb (89%) were low in the diabetic foot patients. As for trace elements, levels of iron (97%) and zinc (95%) were low in the patients, but levels of magnesium and copper were usually normal or high. There were no differences in demographic characteristics based on wound healing rate. However, when compared to normal adult values, diabetic foot patients in our data exhibited significantly lower levels of hemoglobin, total protein, albumin, iron, zinc, copper, and HDL cholesterol. When compared based on age and length of hospital stay, hemoglobin levels were significantly lower in both the older age group and the group with longer hospital stays. Conclusions: Serum levels of albumin, Hb, iron, and zinc were very low in most diabetic foot patients. These low values may have a negative relationship with wound healing. Nutrient replacements are necessary for wound healing in diabetic foot patients.