Zinc Levels Research Articles

Evaluation of polypropylene CSF low-bind collection tubes for trace metal contamination.

Due to the ability of metal ions to cross the blood-brain barrier, there has been interest in analyzing cerebrospinal fluid (CSF) for trace element concentrations to investigate possible correlations with neurodegenerative diseases. In this study, Sarstedt polypropylene CSF collection tubes were analyzed to determine the contamination levels of aluminum, titanium, chromium, manganese, cobalt, nickel, molybdenum, gadolinium, vanadium, arsenic, cadmium, mercury, lead, thallium, selenium, copper, zinc, and iron. Sarstedt polypropylene CSF collection tubes from 2 separate lots (n = 10 per lot) were filled with a 2 mL aliquot of a CSF pool with known element concentrations. After 24 hours of leaching at room temperature, all 18 elements were analyzed via inductively coupled plasma mass spectrometry (ICP-MS). Results were subtracted from the initial pool concentration to determine contamination levels. No detectable contamination above the assay limit of detection was found in 11 analytes. Molybdenum and selenium contamination was measured in all tubes, and aluminum, titanium, manganese, thallium, and zinc had minimal levels of sporadic detectable contamination in 25% or fewer of the tubes tested. Sarstedt polypropylene CSF tubes are an acceptable collection tube for the analysis of most assessed metals in CSF.

Nitrogen and zinc supplements can enhance morphology and physiology traits of maize at early growth stages

Several studies emphasized the importance of zinc for better morpho-physiology in plants. The co-application of Zn can reduce N loss by increasing its uptake rates, but whether N level can affect Zn accumulation in different plant organs is not well studied. This experiment aimed to examine the impact of different levels of nitrogen and zinc on the morphology, physiology and nutrient uptake of maize, and whether different N levels would affect Zn accumulation. Two maize genotypes were grown hydroponically. Nitrogen was supplemented in two different concentrations, combined with two Zn concentrations. Zn level did not affect the shoot or the root dry weight of either genotype under optimal N level, but did under reduced N level. Reduced N level resulted in significantly higher root dry weight under both Zn concentrations in both genotypes. Significantly higher nitrogen remobilization rate was found at reduced N level in both genotypes. Optimal N level resulted in higher shoot and root N and Zn contents in both genotypes. The uptake of Zn was enhanced by optimal N compared to reduced N level, where the impact of genotypes was also observable. Reduced N level decreased both optimal and actual photochemical efficiency of photosystem II, and pigment contents of both genotypes. It could be concluded that N and Zn use efficiency is highly dependent on the genetic characteristics of maize genotypes, but optimal N supply is required for better incorporation of Zn into shoot and root tissues.

Association Between the Copper-to-Zinc Ratio and Cardiovascular Disease Among Chinese Adults: A China Multi-ethnic Cohort (CMEC) Study.

The impact of metal exposure on cardiovascular diseases has become an increasingly concerning topic. To date, few studies have investigated the relationship between the copper-to-zinc ratio and CVD (Cardiovascular disease). This China multi-ethnic cohort study explored the association between the copper-to-zinc ratio and CVD in Chinese adults. The study included a sample size of 9878 people. Logistic regression analysis was used to examine the correlation between urinary copper, urinary zinc, and the copper-to-zinc ratio and CVD prevalence. Restricted cubic spline (RCS) analysis was used to investigate the potential dose-response relationships among copper-to-zinc ratio, urinary copper, urinary zinc, and CVD prevalence. In addition, the least absolute shrinkage and selection operator (LASSO) regression method was used to identify significant risk factors associated with CVD, leading to the development of a nomogram. The predictive performance of the nomogram model for CVD was assessed using the receiver operating characteristic (ROC) curve and the area under the curve (AUC). Compared with the copper-to-zinc ratio in Q1, the copper-to-zinc ratio in Q4 was associated with CVD after adjusting for all potential confounders (Model 3) (Q4, odds ratio [OR] 0.608, 95% confidence interval [CI] 0.416-0.889, P = 0.010). After adjusting for all potential confounders (Model 3), urinary copper levels in Q4 were associated with CVD (Q4, odds ratio [OR] 0.627, 95% confidence interval [CI] 0.436-0.902, P = 0.012). No significant difference was found between urinary zinc levels and CVD. The RCS showed a linear dose-response relationship between the copper-to-zinc ratio and CVD (P for overall = 0.01). The nomogram based on the influencing factors examined with LASSO showed good predictive power, and the AUC was 76.3% (95% CI 73.7-78.9%). Our results suggest that there is a significant linear negative correlation between the copper-to-zinc ratio and CVD in Chinese adults and that it has good predictive value for CVD.

Evaluating the serum levels of zinc, copper, magnesium, and 25-hydroxy vitamin D in children with idiopathic drug-resistant epilepsy; a cross-sectional study

BackgroundDrug-resistant epilepsy is defined as failure of seizure control in spite of using 2 or 3 proper antiepileptic drugs in appropriate time. Mineral elements play important roles in neuronal function; it is believed that mineral deficiency may lead to complications through seizure management. In the present study, serum levels of zinc (Zn), copper (Cu), magnesium (Mg), calcium (Ca), and 25-hydroxy vitamin D (Vit D) in drug-resistant-epilepsy (DRE) patients were evaluated and compared with the controlled patients.MethodsIn this cross-sectional study, epileptic patients were included and categorized into two groups of DRE and well-controlled patients. Patients’ serum samples were analysed to evaluate Zn, Cu, Mg, Ca, and Vit D levels. The primary objective was comparison of serum levels of different trace elements between the groups.ResultsSixty-four epileptic children including 33 DRE and 31 well-controlled children entered the study. The DRE children showed a significantly earlier onset of disease compared to the other group (p = 0.014). Comparing the frequency of developmental delay between the groups, the results showed this complication was significantly more frequent in the DRE group (p < 0.001). Concerning serum elements, the results showed a significantly higher concentration of Zn in the well-controlled group than the DRE group (p = 0.007). On the other hand, no significant differences were observed between the groups regarding the means of Vit D, Ca, Cu, and Mg levels (p > 0.05).ConclusionThe results of the present study delineated that drug-resistant epilepsy patients had earlier onset of disease and were at higher risk of neurodevelopmental delay compared with well-controlled-epilepsy patients. A significant lower serum levels of Zn were also observed in drug-resistant-epilepsy patients. This finding may suggest the role of zinc supplementation in help to better control of drug-resistant seizures, as well as, the importance of serum zinc monitoring in epileptic patients.

Impact of agricultural inputs on the abundance of heavy metals (Cu, Zn) in soil, water, and plants in the south of Algeria

he aim of our study is to assess the impact of agricultural inputs on the levels of heavy metals, specifically copper (Cu) and zinc (Zn), in soil, water, and plants in the oasis of El Ghrous, Branis, and Sidi Okba in Algeria. The existence of perilous substances in the surroundings is a significant ecological issue, endangering both human well-being and agricultural output. The overuse of fertilizers and pesticides may be causing heavy metals build-up. The aim will be to highlight a better understanding of the concentrations of copper (Cu) and zinc (Zn), considering as detrimental contaminants, after extensive utilization of agricultural inputs. In order to examine the levels of trace metals (TMs), we performed additional tests by comparing field samples using metal analysis techniques that involved ammonium acetate and EDTA, and detection by atomic absorption spectrophotometry. Our findings suggest that in soils affected by fertilizers and phytosanitary agents, the concentrations of Cu++ and Zn++ are below levels that would cause toxicity. Indeed, the levels of Cu++ are measured at 6.14 µg/g and the levels of Zn++ are measured at 4.97 µg/g. Nevertheless, the copper contents in irrigation waters from sites 01 and 02 beyond the levels that are considered dangerous. Similarly, copper levels in plants are categorized as hazardous. The findings emphasize the significance of implementing integrated pest management strategies in order to reduce the negative consequences of excessive use of agricultural inputs. Additionally, our study imposes the need to closely monitoring agricultural practices in order to mitigate environmental and public health hazards. Implementing sustainable farming practices and employing effective resource management strategies is crucial for safeguarding agricultural ecosystems and guaranteeing sustainable food production in the long run. Adopting an all-encompassing strategy is crucial for preserving the well-being of both the ecosystem and the populace.

Effect of Zinc and Vitamin E on Blood Testosterone and Inflammatory Markers in Male Patients Undergoing Heart Surgery

Background: Zinc and vitamin E affect the metabolism of testosterone and inflammatory factors. We aimed to evaluate the effect of zinc and vitamin E supplementation on plasma testosterone levels and inflammatory markers in patients undergoing coronary artery bypass graft (CABG) surgery. Methods: This study was a secondary analysis of a previously published randomized controlled trial in a subsample of male patients undergoing CABG surgery. Patients in the zinc-vitamin E group (n = 27) received oral zinc (120 mg) and vitamin E (1200 international units) one day prior to surgery, followed by 30 mg of zinc and 200 units of vitamin E per day for three weeks after surgery. Patients in the control group (n = 25) received a placebo. Plasma levels of total testosterone, cortisol, interleukin-6 (IL-6), and white blood cell toll-like receptor-4 (TLR-4) gene expression were determined at three-day and three-week intervals following surgery. Changes in these markers were analyzed using a repeated-measures analysis of variance. Results: A comparison of the groups revealed no significant difference in the concentration of plasma total testosterone levels (P = 0.059) or cortisol. Three weeks following the surgical procedure, a positive correlation was observed between the change in plasma zinc concentrations and the change in plasma testosterone levels (r = 0.32; P = 0.025). The administration of zinc and vitamin E supplements resulted in a reduction in plasma IL-6 levels on postoperative day 3 (P = 0.025), while no significant effect was observed in week 3 (P = 0.091). The expression of the TLR-4 gene in WBCs was found to be lower in the zinc-vitamin E group compared to the placebo group on day 3 (P = 0.051) and week 3 (P = 0.025). Conclusions: The administration of zinc and vitamin E to patients undergoing CABG was associated with a relative improvement in postoperative inflammatory markers. Plasma zinc levels demonstrated a correlation with testosterone levels, suggesting a potential avenue for further research in these patients.

Blood and Serum Se and Zn Levels and 10-Year Survival of Patients after a Diagnosis of Kidney Cancer.

The aim of the project was to evaluate the association between selenium (Se) and zinc (Zn) levels in blood and serum and kidney cancer mortality. In a prospective group of 284 consecutive, unselected patients with kidney cancer, we evaluated their 10-year survival rate in relation to the levels of Se and Zn in their blood and serum. Micronutrient levels were measured using an inductively coupled plasma mass spectrometer. Patients were divided into quartiles based on the distribution of Se and Zn levels arranged in increasing order. The following variables were taken into account in the multivariable models: age at diagnosis, gender, smoking, type of surgery and histopathological examination results. We observed a statistically significant association of all-cause mortality when subgroups with low blood selenium levels were compared to patients with high selenium levels (HR = 7.74; p < 0.001). We found, in addition, that this correlation was much stronger when only men were assessed (HR = 11.6; p < 0.001). We did not find a statistically significant association for zinc alone. When we combined selenium and zinc levels (SeQI-ZnQI vs. SeQIV-ZnQIV), we observed the hazard ratio for kidney cancer death to be 12.4; p = 0.016. For patients in the highest quartile of blood zinc/selenium ratio, compared to those in the lowest, the HR was 2.53; p = 0.008. Our study suggests that selenium levels, combined selenium and zinc levels (SeQI-ZnQI vs. SeQIV-ZnQIV) and zinc-to-selenium ratio (Zn/Se) are attractive targets for clinical trials aimed at improving the survival of kidney cancer patients.

Effect of Zinc Application on Growth, Yield, Zinc uptake and Zinc Use Indices of Rice

A field experiment was conducted at the agriculture farm, Palli Siksha Bhavana, Visva-Bharati, Birbhum, West Bengal, India during kharif (rainy) seasons (June–September) of 2016 and 2017 to study effect of zinc application on growth, yield and nutrient uptake of rice. The experiment was laid out in split plot design replicated thrice, consisting two main plot treatments i.e. crop establishment methods (CEMs) and seven sub-plot treatments (levels of zinc and methods of application). Two CEMs were direct seeded rice (DSR) and transplanted puddled rice (TPR) and seven levels of zinc and methods of application were namely control (Zn0), Seed coating of Zn @ 1250 mg kg-1 (Zn1), Seed coating of Zn @ 2500 mg kg-1 (Zn2), Seed coating of Zn @ 3750 mg kg-1 (Zn3), two foliar sprays @ 1050 mg kg-1 (Zn4), three foliar spray @ 1050 mg kg-1 (Zn5), 2500 mg kg-1 seed coating+2-foliar spray @ 1050 mg kg-1 (Zn6). In respect of yield attribute and yield, Zn6 resulted significantly highest number of Panicle m-2 as well as number of filled grains panicle-1. Zn3 though recorded 5.7% more grain yield than Zn6 but later Zn6 resulted highest zinc concentration and uptake. Zn1 recorded highest zinc use indices. Zn1 resulted much highest Zn use efficiency than foliar application and combined application of seed coating and foliar spray. Correlation between Zn levels and Zn concentration in both grain and straw of rice was highly positive; very highly positive correlation was recorded with the Zn levels and Zn uptake by both grain and straw.

Assessment of zinc sources and levels for urea coating to achieve higher productivity and soil fertility in spring maize (Zea mays)

An experiment was conducted during 2021 and 2022 at the research farm of ICAR-Indian Institute of Maize Research, Ludhiana, Punjab to study the efficacy of zinc oxide and zinc sulphate for urea coating to achieve higher productivity in maize (Zea mays L.). Experiment was laid out in a randomized block design (RBD) comprised of 11 treatments, viz. no Zn; 0.05% of Zn through ZnO; 0.12% of Zn through ZnO; 0.24% of Zn through ZnO; 0.47% of Zn through ZnO; 0.95% of Zn through ZnO; 0.26% of Zn through ZnSO4; 0.52% of Zn through ZnSO4; 1.03% of Zn through ZnSO4; 2.07% of Zn through ZnSO4; and 4.15% of Zn through ZnSO4, replicated thrice. Results indicated that grains/cob and cob weight/plant recorded higher with 1.03%, 2.07% and 4.15% of Zn through ZnSO4 compared to no Zn. Zn at 2.07% (5.68 and 6.36) and 4.15% (5.71 and 6.37) through ZnSO4 recorded higher grain yield followed by 0.95% of Zn (5.50 and 6.23) through ZnO and 1.03% (5.42 and 6.25) of Zn through ZnSO4 in 2021 and 2022. A higher Zn content in grain was registered with 4.15% (27.70 and 27.87 mg/kg) and 2.07% (26.33 and 27.67 mg/kg) of Zn through ZnSO4. Overall, Zn at 2.07% through ZnSO4 was found suitable for urea coating to enhance productivity, soil enzymatic activities and residual soil fertility in spring maize.

Decreased vascular contraction and changes in oxidative state in middle–aged Wistar rats after exposure to increased levels of dietary zinc

Both copper and zinc are known to be important for maintaining health, but most research has focused on deficiencies of these elements. Recent studies have shown that high levels of Cu can be toxic, especially to the cardiovascular (CV) system. However, little research has been done on the effects of higher levels of Zn on the CV system. In this study, male Wistar rats aged 12 months were given a diet with twice the recommended daily allowance of zinc (31.8 mg/kg of diet) and compared to a control group (15.9 mg/kg of diet) after 8 weeks. Blood plasma and internal organs of both groups were examined for levels of copper, zinc, selenium and iron, as well as several key enzymes. Aortic rings from both groups were also examined to determine vascular functioning. There were very few changes in the vascular system functioning after chronic exposure to zinc, and only one enzyme, heme oxygenase–1 (HO–1) was elevated, whereas vascular contraction to noradrenaline decreased with no changes in vasodilation to acetylcholine. Of the micronutrients, zinc and selenium were elevated in the blood plasma, while copper decreased. Meanwhile, the total antioxidant status increased. These were not observed in the liver. Therefore, it is proposed that there is a mechanism in place within the vascular system to protect against the overproduction of heme, caused by chronic zinc exposure.

The gut-immune-liver axis in patients with alcohol use disorder and clinically low serum zinc levels.

Alcohol use disorder (AUD) with chronic and heavy alcohol consumption causes alcohol-associated liver disease (ALD). Early-stage ALD exhibits dyshomeostasis of zinc. We investigated the role of zinc deficiency in gut-barrier dysfunction, proinflammatory response, hepatocyte injury, and death, as well as potential sex differences in AUD patients. Thirty-nine male and female AUD patients were grouped by normal [≥71 μg/dL (Group 1, number (n) = 26)] and low [<71 μg/dL (Group 2, n = 13)] serum zinc levels. Demographics, alcohol intake markers [Lifetime Drinking History (LTDH), heavy drinking days in the past 90-days (HDD90), total drinks in the past 90-days (TD90), number of drinking days in the past 90-days (NDD90), average drinks per day in the past 90 days (AvgDPD90)] were collected. Blood samples were tested for complete blood count (CBC), comprehensive metabolic panel (CMP), coagulation markers, gut-barrier dysfunction markers, cytokines, and hepatocyte death markers. Group 2 females exhibited lower LTDH than Group 2 males (p = 0.028), but higher recent drinking. Aspartate transaminase: alanine transaminase (AST:ALT) ratio was higher (p = 0.049) in Group 2 males compared to Group 1 males. Overall, Group 2 showed threefold higher interleukin 8 (IL-8) levels than Group 1 (p = 0.92); these were sevenfold higher in Group 2 females than Group 1 females. Group 2 females also had higher K18M65, but lower K18M30 than Group 1 females. Necrotic type of cell death (K18M65) was well-described only in Group 2 by the arrangement of lipopolysaccharide (LPS), soluble cluster of differentiation 14 (sCD14), and tumor necrosis factor alpha (TNF-α) (R2 = 0.633, p = 0.037). Our findings demonstrated the role of the gut-immune-liver axis in describing hepatocyte injury and death in zinc-deficient AUD patients. These patients represented an arrangement of gut-barrier dysfunction and an exacerbated immune response. Shift in the cell-death mechanism from apoptosis in zinc-replete females to necrosis in zinc-deficient females suggests a subclinical to clinical transition of ALD associated with zinc status.

Serum levels of zinc, folate, and vitamin B12 in healthy children aged 3-12 months: Is routine screening necessary?

To retrospectively assess the serum levels of zinc, folate, and vitamin B12 in healthy children aged between 3-12 months. This study includes healthy children aged between 3-12 months who presented to the pediatric outpatient clinics of Ankara Bilkent City Hospital, Ankara, Turkey, between January 2020 and July 2022. The levels of serum zinc, folate, and vitamin B12 were evaluated retrospectively. Of the 495 patients enrolled in our study, 248 (50.1%) were female. The median age of the patients was 10 (range: 7-12) months. Zinc deficiency was detected in 24 (4.8%) patients, and vitamin B12 deficiency was found in 49 (9.8%) patients. No folate deficiency was observed in any patient. There was no significant correlation between the patients' height and weight percentiles and their serum levels of zinc, folate, and vitamin B12 (p>0.05 for each). In conclusion, we do not recommend routine screening for zinc, folate, and vitamin B12 levels in children under 12 months of age without active issues or chronic diseases due to the associated costs. We propose that evaluating serum levels of zinc, folate, and vitamin B12 is a more appropriate clinical approach in children at risk for micronutrient deficiencies and in selected patient groups.

The Interaction of Zinc as an Essential Trace Element with Leishmania Parasites: A Systematic Review.

The trace element of zinc (Zn) has shown great effectiveness in control of leishmaniasis infection. Hence, the present study conducted a systematic review of in vitro and in vivo studies evaluating the zinc effect in the treatment or prevention of leishmaniasis. A systematic literature search was performed of all articles published in PubMed, SciELO, ScienceDirect, Scopus, Google Scholar, and Web of Science databases (1997-2023). The search terms were "zinc" OR "cutaneous leishmaniasis (CL)" OR "visceral leishmaniasis (VL)". Initial search yielded 89 citations, and 59 subjects were included. Data showed the zinc serum level in CL patients was lower than controls. Also, in vitro studies of zinc were more effective against L. tropica and L. major promastigotes compared to the amastigotes. Moreover, in vivo studies did not show destructive effects of zinc on the mammalian cell viability like macrophages. Furthermore, zinc depletion by specific chelators affected L. donovani survival and growth through promoting apoptosis and reactive oxygen species-dependent mechanisms. The serum level determination of zinc could be useful for estimating the leishmaniasis pathophysiology. Environmentally or genetically determined increases in zinc levels might augment resístanse to CL. In contrast, zinc depletion using a zinc-specific chelator could be effective treatment of VL in endemic areas.

Influence of the Saccharomyces cerevisiae-based probiotic complex on gut microbiota, serum biochemistry, and circulating trace element and mineral levels in lactating dairy cows.

The existing data demonstrate that gut microbiota is involved in regulating mineral metabolism in cattle, although the data are quite contradictory. The study aimed to evaluate Saccharomyces cerevisiae-based probiotic's effects on gut microbiota, systemic metabolism, and dairy cows' essential trace element and mineral body burden. Fifteen cows received a daily supplement of a 50 g S. cerevisiae-based probiotic, fortified with methionine, choline, eugenol, cinnamaldehyde, and Capsicum oleoresin, for a month. 16S metagenomic sequencing was used to evaluate the taxonomic features of fecal microbiota. Serum trace elements and minerals levels were determined through inductively coupled plasma mass spectrometry. Supplementation with S. cerevisiae-based probiotic complex significantly increased alpha and beta diversity, as well as the abundance of Mediterranea and Clostridium IV within the Bacillota phylum, whereas that of Bacteroidota and specifically unclassified Bacteroidales and unclassified Oscillospiraceae decreased. Following probiotic supplementation with the S. cerevisiae-based complex, gut microbiota modulation led to a significant boost in circulating levels of calcium, copper, selenium, and zinc. Creatinine levels decreased while total cholesterol levels increased within normal limits in the serum analysis. The observed improvement in trace elements and minerals in dairy cows might be due to changes in intestinal microflora caused by supplementation. Therefore, probiotic supplementation in cattle may be considered a potential tool for improvement of mineral nutrition in cattle. However, the influence of probiotic treatment and modulation of mineral metabolism on milk productivity and overall performance in cattle is yet to be estimated.

Multiple metal exposures associate with higher amyotrophic lateral sclerosis risk and mortality independent of genetic risk and correlate to self-reported exposures: a case-control study

BackgroundThe pathogenesis of amyotrophic lateral sclerosis (ALS) involves both genetic and environmental factors. This study investigates associations between metal measures in plasma and urine, ALS risk and survival and exposure...

Investigation of the Relationship Between Heavy Metals (Cadmium, Arsenic, and Lead) and Metallothionein in Multiple Sclerosis.

Multiple sclerosis (MS) is one of the most common neurological disorders. Metals are important for the maintenance and preservation of homeostasis and dysregulated metal homeostasis has an impact on neurodegeneration. Environmental factors are considered to contribute to MS risk and progression. Heavy metals such as arsenic (As), cadmium (Cd), and lead (Pb) are widely found in the environment and because of their toxic nature, they pose a great danger to human health. Metallothioneins (MTs) play important roles in metal homeostasis and detoxification of heavy metals. The aim of this study was to investigate the relationship between levels of heavy metals (As, Cd, and Pb) andMT levels in MS patients and also to assess the oxidative stress status of patients. Fifty subjects (20 healthy subjects and 30 MS patients) were included. Demographic characteristics of the patients, plasma MT levels, blood Cd, As, and Pb levels, as well as iron (Fe), copper (Cu), and zinc (Zn) levels, were determined. Malondialdehyde (MDA) levels were investigated as a marker of oxidative stress. MT levels were slightly higher in the MS group (p > 0.05). As Cd and Pb levels were significantly higher in the control subjects. MDA levels were significantly higher in MS patients. Our results support the relevance of MT and MDA levels in MS. Further clinical studies with larger cohorts will provide more insights into these factors.

Metals link to diabetes: Insights from a national cross‐sectional investigation

Diabetes and impaired fasting glucose (IFG) are significant global health concerns. However, the potential effects of mixed heavy metal exposure on these conditions remain underexplored. This study aims to investigate the combined effects of multiple metals on diabetes risk and explore the mediating role of Body Mass Index (BMI) in rural China. A cross-sectional analysis involved 2313 adults from 12 provinces in rural China. Urinary levels of zinc (Zn), chromium (Cr), nickel (Ni), cadmium (Cd), and lead (Pb) were quantified using inductively coupled plasma mass spectrometry. Fasting blood glucose (FBG) levels were measured with an automatic biochemical analyzer. Logistic regression models and the Bayesian Kernel Machine Regression (BKMR) model were used to examine associations and interactions. Mediation analysis was performed to assess the role of BMI. The results of our study indicate that there is a significant association between urinary Zn (OR = 2.38, 95 % CI: 1.57, 3.60), Cr (OR = 1.24, 95 % CI: 1.31, 1.61), and Ni (OR = 1.51, 95 % CI: 1.05, 2.18) and the diabetes risk. The study revealed that exposure to Ni amplified the associations between Zn, Cr and diabetes/IFG risk. Additionally, BMI was identified as a significant mediator in the relationship between metal exposure, particularly Cr/Cd, and diabetes risk. These findings reveal a complex link between multiple metals, such as Zn, Cr, and Ni, and diabetes risk, and emphasize the potential opposite mediating effects of BMI in different metal-induced diabetes mechanisms. Further investigation of these mechanisms is warranted.

Influence of Seed Treatment with Salicylic Acid on Growth, Antioxidant Enzyme Activity, and Proline Content in Wheat Leaves at Excessive Zinc Level in the External Environment

Influence of Seed Treatment with Salicylic Acid on Growth, Antioxidant Enzyme Activity, and Proline Content in Wheat Leaves at Excessive Zinc Level in the External Environment

Causal association of micronutrients and supplements with pressure ulcer: A Mendelian randomization study.

Pressure ulcer (PU) is known to be associated with abnormalities of micronutrient status. However, to date, it is not clear whether a causal relationship exists between circulating levels of micronutrients and their supplementations and PU. A two-sample Mendelian randomization (MR) study was conducted using summary statistics from Genome-Wide Association Studies (GWAS). Genetic instrumental variables (IVs) for 13 micronutrients were identified from a GWAS of 67582 participants, IVs for supplement zinc were acquired from 18826 cases and 44255880 controls, and IVs for PU were obtained from 663 PUs and 207482 controls. The MR analysis was conducted using the MR base platform. The main analysis method was inverse variance weighted (IVW) analysis, supplemented by MR Egger, Weighted median, Weighted mode, and Simple mode analyses. Heterogeneity was assessed using Cochran's Q statistic for MR-IVW and Rucker's Q statistic for MR-Egger. Pleiotropy was determined bythe MR-Eggerregression. Sensitivityanalysiswasconductedusingthe leave-one-outmethod, and publication bias was evaluated using funnel plots. Genetically predicted lower circulating zinc levels were found to be causally linked to the development of PU (OR=0.758, 95%CI 0.583-0.987, P=0.040). However, there was no significant evidence of a causal relationship between supplemental zinc intake and PU development (P>0.05). Additionally, no causal association was observed between the other circulating micronutrients and the occurrence of PU. Furthermore, there was no indication of horizontal pleiotropy or heterogeneity among genetic variants (P>0.05), and the robustness of the findings was confirmed through leave-one-out tests and funnel plots. Our findings indicate a potential causal association between circulating zinc levels and decreased risk of PU. However, zinc supplementation did not demonstrate a significant reduction in the risk of PU. Further research is warranted to elucidate the underlying mechanisms through which zinc influences the pathogenesis of PU and evaluate the efficacy of zinc supplementation in the prevention and management of PU.

Unique expression and critical role of metallothionein 3 in the control of osteoclastogenesis and osteoporosis

Bone homeostasis is maintained by an intricate balance between osteoclasts and osteoblasts, which becomes disturbed in osteoporosis. Metallothioneins (MTs) are major contributors in cellular zinc regulation. However, the role of MTs in bone cell regulation has remained unexplored. Single-cell RNA sequencing analysis discovered that, unlike the expression of other MT members, the expression of MT3 was unique to osteoclasts among various macrophage populations and was highly upregulated during osteoclast differentiation. This unique MT3 upregulation was validated experimentally and supported by ATAC sequencing data analyses. Downregulation of MT3 by gene knockdown or knockout resulted in excessive osteoclastogenesis and exacerbated bone loss in ovariectomy-induced osteoporosis. Transcriptome sequencing of MT3 knockdown osteoclasts and gene set enrichment analysis indicated that the oxidative stress and redox pathways were enriched, which was verified by MT3-dependent regulation of reactive oxygen species (ROS). In addition, MT3 deficiency increased the transcriptional activity of SP1 in a manner dependent on intracellular zinc levels. This MT3-zinc-SP1 axis was crucial for the control of osteoclasts, as zinc chelation and SP1 knockdown abrogated the promotion of SP1 activity and osteoclastogenesis by MT3 deletion. Moreover, SP1 bound to the NFATc1 promoter, and overexpression of an inactive SP1 mutant negated the effects of MT3 deletion on NFATc1 and osteoclastogenesis. In conclusion, MT3 plays a pivotal role in controlling osteoclastogenesis and bone metabolism via dual axes involving ROS and SP1. The present study demonstrated that MT3 elevation is a potential therapeutic strategy for osteolytic bone disorders, and it established for the first time that MT3 is a crucial bone mass regulator.