Tissue Lipid Peroxidation Levels Research Articles

Protective effect of panaxydol against repeated administration of aristolochic acid on renal function and lipid peroxidation products via activating Keap1-Nrf2/ARE pathway in rat kidney.

Panaxydol (PX), a polyacetylenic compound isolated from the roots of Panax notoginseng, is found to possess various biological functions. However, its protective effects against aristolochic acid (AA)-induced renal injury have not been elucidated yet. The present study was undertaken to elucidate the renoprotective effect of PX on Wistar male rats via activating Keap1-Nrf2/ARE pathway. Experimental animals were randomized into four groups, such as control group, I/R group, AA (5 mg/kg/d; ip for 10 days), and AA-induced rats treated with PX (10 and 20 mg/kg/d; po for 20 days). At the end of the experimental period, the rats were killed, and the biochemical parameters denoting renal functions were evaluated; histological analysis displaying the renal tissue architecture, real-time quantitative reverse-transcription polymerase chain reaction, and immunohistochemistry (IHC) analysis of Keap1-Nrf2/ARE genes were elucidated. The results demonstrated that the rats administered with AA displayed a significant increase in the blood urea nitrogen level with an increased urine creatinine and protein excretion. Also, the serum levels of urea, uric acid, and albumin levels were increased. Furthermore, the histological evaluation denoted the cellular degeneration with increased tissue lipid peroxidation levels. In contrast, rats administered with PX significantly prevented the tissue degeneration with improved antioxidant levels. Conversely, PX treatment increased the messenger RNA expression of Nrf2, NQO1, HO-1 with an attenuated expression of 4HNE and NOX-4 levels in IHC analysis. Thus, the results of the present study suggest that PX could suppress AA-induced renal failure by suppressing oxidative stress through the activation of Keap1-Nrf2 signaling pathway.

Therapeutic investigation of quercetin nanomedicine in a zebrafish model of diabetic retinopathy

Diabetic retinopathy (DR) is progressive damage to the retina and it's caused by damage to the blood-retinal barrier. Quercetin has pleiotropic action like anti-oxidant, regulation cell cycle &vascular integrity, and preventive effect of neuroinflammation. The present study is designed to investigate the nano-formulation of quercetin (NQ) in a zebrafish model of DR. The DR was developed by a single intraperitoneal injection of streptozotocin (STZ; 350 mg/kg). The acceleration of retinopathy was made on 7 days of diabetic zebrafish by intravitreal injection of STZ (20 μL of 7 % w/v of STZ stock solution). The treatment of NQ (5 and 10 mg/kg; i.p.) was administered for 21 consecutive days. The reference control i.e., dexamethasone (DEX, 10 mg/kg; i.p.) was also administered for 21 consecutive days. The sign of DR was assessed by eyeball/body weight ratio, eyeball weight, optomotor response (OMR), startle response (SR), phototactic response (PTR), and escape response (ER). Furthermore, the biochemical changes like plasma glucose and homocysteine (HCY) levels; and eye retinal tissue lipid peroxidation, reduced glutathione (GSH), and arginase reductase (AR) activity levels were assessed. The NQ found to attenuate the effect of STZ induced DR along with the regulation of biochemical abnormalities. And, it also comparable with reference drug treatment i.e., DEX treated group. Hence, NQ can be used for the treatment of diabetic associated retinopathy and neurosensory disorder visits anti-hyperglycemic, regulation of homocysteine pathway, reduction of lipid peroxidation, and free radical scavenging actions.

Effect of Garlic extract on physiological and biochemical parameters of Rhamdia quelen and their response to an infestation with Ichthyophthirius multifiliis

The aim of the study was to investigate the effect of garlic extract on hematological, immune and metabolic systems of Rhamdia quelen juveniles and the animals’s resistance to Ichthyophthirius multifiliis. The fishes were fed twice daily during 77 days with diets containing 0%, 0.5%, 2.5% and 5% of garlic extract. After that, half of the animals of each treatment were submitted to the challenge with the parasite I. multifiliis, maintaining the diets. The other half of the animals were maintained in the original system receiving the dietary supplementation. The use of garlic extract in the diet did not promote significant changes in glycaemia, hepatic glycogen content, tissue and plasma aminotransferase activities, and tissue lipid peroxidation levels. On the other hand, it stimulated the synthesis of muscle glycogen and promoted a reduction of hepatic catalase activity. In terms of hematological and immune systems, the use of garlic extract increased the number of red blood cells, but reduced hemoglobin content. Furthermore, it was not able to change the total number of leukocytes and thrombocytes, but it promoted an increase in the number of basophils and reduced the monocyte count of the animals. The dietary supplementation with garlic extract did not act as a growth promoter for silver catfish juveniles, did not influence the basal metabolic homeostasis, immune or hematological responses of the fishes and did not improve the resistance to the infestation with the I. multifiliis in the concentrations and periods studied

Specific Features of Antioxidant Glutathione System in Tissues of the Black Sea Bivalve Mollusk Cerastoderma glaucum (Cardiidae)

The antioxidant glutathione system (AGS) and lipid peroxidation (LP) level in tissues of the Black Sea cockle Cerastoderma glaucum (Bruguiere, 1789) have been investigated and compared with those of other Black Sea bivalve mollusks with high resistance to oxidative stress (OS), namely, mussel Mytilus galloprovincialis (Lamarck, 1819) and anadara Anadara kagoshimensis (Tokunaga, 1906). The activity of glutathione peroxidase (GP) and glutathione reductase (GR) and the level of reduced glutathione (GSH) and LP products are measured in the hepatopancreas, gills, and foot of the mollusks. The parameters of AGS in C. glaucum are found to have tissue specificity. The hepatopancreas has the highest GP activity, 1.7–4.2 times higher than in the gills and foot of the mollusk. The gills have the lowest GSH level (by a factor of 2.1–2.3 times lower) and the highest GR activity (3.1–5.8 times higher) when compared to other tissues. In the cockle foot, the level of GSH is close to that in hepatopancreas and the activity of GP and GR is by a factor of 1.7–4.2 lower than in other tissues. In all studied tissues of C. glaucum, the LP level is significantly lower, i.e., by a factor of 3.2–16.3, when compared with that in mussel and anadara.

Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice.

Intestinal ischemia/reperfusion (I/R) injury occurs during transplantation, mesenteric arterial occlusion, trauma and shock, causing systemic inflammation, multiple organ dysfunction and high mortality. Pancreatic secretory trypsin inhibitor (PSTI), a serine protease inhibitor expressed in gut mucosa may function as a mucosal protective/repair peptide. We examined whether PSTI affected mesenteric I/R-induced injury. Hypoxia/normoxia (H/N) caused 50% drop in cell viability of AGS, RIE1 and Caco-2 cells but PSTI (10 μg/ml) given prior- or during-hypoxic period improved survival by 50% (p<0.01). Similarly, Caco-2 monolayers exposed to H/N had 300% increase in transepithelial permeability, PSTI truncated this by 50% (p<0.01). Mice underwent mesenteric I/R by clamping jejunum, causing severe mucosal injury, increased apoptotic markers and 3-fold increases in plasma IL-6, IL1β, TNFα, and tissue lipid peroxidation (MDA) and inflammatory infiltration (MPO) levels. Lungs showed similar significant injury and inflammatory infiltrate markers. Smaller increases in MDA and MPO were seen in kidney & liver. PSTI (20 mg/kg) reduced all injury markers by 50–80% (p<0.01). In vitro and in vivo studies showed PSTI reduced pro-apoptotic Caspase 3, 9 and Baxα levels, normalised Bcl2 and caused additional increases in HIF1α, VEGF and Hsp70 above rises caused by I/R alone (all p<0.01). PSTI also prevented reduction of tight junction molecules ZO1 and Claudin1 (all p<0.01) but did not affect increased ICAM-1 caused by I/R in gut or lung. PSTI may be a useful clinical target to prevent I/R injury.

Carbohydrate-restricted diet alters the gut microbiota, promotes senescence and shortens the life span in senescence-accelerated prone mice

This study examined the effects of a carbohydrate-restricted diet on aging, brain function, intestinal bacteria and the life span to determine long-term carbohydrate-restriction effects on the aging process in senescence-accelerated prone mice (SAMP8). Three-week-old male SAMP8 were divided into three groups after a week of preliminary feeding. One group was given a controlled diet, while the others fed on high-fat and carbohydrate-restricted diets, respectively. The mice in each group were further divided into two subgroups, of which one was the longevity measurement group. The other groups fed ad libitum until the mice were 50 weeks old. Before the test period termination, passive avoidance test evaluated the learning and memory abilities. Following the test period, serum and various mice organs were obtained and submitted for analysis. The carbohydrate-restricted diet group exhibited significant decrease in the survival rate as compared to the other two diet groups. The passive avoidance test revealed a remarkable decrease in the learning and memory ability of carbohydrate-restricted diet group as compared to the control-diet group. Measurement of lipid peroxide level in tissues displayed a marked increase in the brain and spleen of carbohydrate-restricted diet group than the control-diet and high-fat diet groups. Furthermore, notable serum IL-6 and IL-1β level (inflammation indicators) elevations, decrease in Enterobacteria (with anti-inflammatory action), increase in inflammation-inducing Enterobacteria and lowering of short-chain fatty acids levels in cecum were observed in the carbohydrate-restricted diet group. Hence, carbohydrate-restricted diet was revealed to promote aging and shortening of life in SAMP8.

EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS

Objective: To evaluate hepatoprotective effects of ethanol extract of aerial part of Blumea lacera (BLEE) against ethanol-induced hepatotoxicity in rats.&#x0D; Methods: The in vivo antioxidant activity of BLEE was assessed by determining the tissue glutathione (GSH) and lipid peroxidation (LPO) levels. The BLEE at the doses of 200 and 400 mg/kg and silymarin 100 mg/kg administered to the ethanol challenged rats. The effects of BLEE and silymarin on Physical and Biochemical Parameters were measured. Similarly, histopathological changes of the liver were studied.&#x0D; Results: The BLEE showed in vivo antioxidant activity. A significant (P&lt;0.001) decrease in SGOT, SGPT, ALP, total and direct bilirubin was observed in BLEE treated group at doses i.e. 200 mg/kg and 400 mg/kg as compared to intoxicated group. Liver damage in animal pretreated with BLEE was minimal with distinct preservation of structures and the architectural frame of the hepatic cells.&#x0D; Conclusion: These findings demonstrated the hepatoprotective effects of BLEE against ethanol-induced liver damage.

Antioxidant and hepatopreventive effects of Cassia angustifolia extract against carbon tetrachloride-induced hepatotoxicity in rats

The present study aimed to investigate the preventive effects of methanol fraction from Cassia angustifolia leaf extract (MECA), associated with its phytochemical content, on CCl4-induced hepatic toxicity in adult rats. In the controls, CCl4 induced an increase of serum biochemical parameters and triggered oxidative stress in the liver. MECA caused significant reductions in CCl4-elevated levels of hepatic markers, total cholesterol, triglycerides, and low-density lipoprotein and increased the level of high-density lipoprotein compared to the CCl4 group. Moreover, pretreatment with the MECA produced significant reductions in lipid peroxidation (thiobarbituric acid reactive substances) and protein carbonyl level in liver tissues as compared with the untreated group. The formation of pathological hepatic lesions was strongly prevented by MECA. Overall, this study suggests that administration of MECA has a high potential to quench free radicals and alleviate CCl4-induced hepatotoxicity in rats.

Influence of some β-lactam drugs on selected antioxidant enzyme and lipid peroxidation levels in different rat tissues

Antioxidant enzymes play an important role in body defense and free radical removal. Cephalosporins are β-lactam antibiotics. In this work, the effects of cefazolin, cefuroxime and cefoperazone which are cephalosporins on some selected antioxidant enzyme and levels of malondialdehyde (MDA) as lipid peroxidation product were investigated in kidney, liver, and brain tissues of albino female rats. Ninety-six albino rats were randomly divided into 16 groups of equal number (n = 6). 50 mg/kg cefazolin, 25 mg/kg cefuroxime, and 100 mg/kg cefoperazone were injected intraperitoneally to the groups (5th–8th and 9th–12th, and 13th–16th groups), respectively. The changes in glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD), and glutathione peroxidase (GSH-Px) levels were studied in each time point group and a time-dependent manner (at the 1st, 3rd, 5th and 7th hour). In addition, MDA levels were examined in all the tissues. The drugs evaluated in this study had different effects on the same enzyme in different tissues depending on time. MDA levels especially in cefazolin and cefoperazone experiments were lower in all the tissues; however, MDA levels were higher in brain and kidney tissues in the cefuroxime groups in a time-dependent manner (p < 0.05). These results revealed the complex effects of the tested drugs on different tissues at different time points. Therefore, the dose and use of these drugs should be adjusted correctly.

Imipenem toxicity in male reproductive organs as a result of inflammatory microenvironment and oxidative stress in germinal cells

Imipenem is a beta-Lactam antibiotic characterized by a broad spectrum of activity. It is prescribed to treat severe infections. Our goal is to investigate toxicity induced in male rat reproductive systems following exposure to this drug (15, 50 or 100 mg/kg) compared to gentamicin (50 mg/kg) treatment.Effects of imipenem on reproductive organ weights, histoarchitecture, sperm parameters, and oxidative stress parameters were evaluated. Serum testosterone levels were measured. Apoptosis and inflammatory behaviors were investigated by immunohistochemical proteins expression analysis of apoptosis regulator BAX (Bax), B-cell lymphoma 2 (Bcl-2), and interleukin-1 beta (IL-1 beta) in testis.Results showed a significant decrease in male fertility parameters including sperm count, sperm motility, reproductive organ weights and serum testosterone levels after imipenem administration as compared to the control and gentamicin treated groups. Increased sperm abnormality was significant in animals treated with high doses of imipenem. Oxidative stress analysis revealed an expressed increase in lipid peroxidation and carbonyl groups levels in testicular tissues compared to control. Similar results were observed with superoxide dismutase and catalase activities from testicular tissues. In addition, severe testicular lesions were observed in the seminiferous tubules as well as important impairments in spermatogenesis testifying an inflammatory microenvironment confirmed by the intensive expression of IL1-beta and Bax protein by germinal cells and Bcl-2 by Leydig cells.In conclusion, imipenem treatment with high doses was found to lead to oxidative stress in male reproductive organs and an inflammatory microenvironment leading to spermatogenesis dysfunction and histopathological changes in the testis.

The Investigation of the Antitumor Agent Toxicity and Capsaicin Effect on the Electron Transport Chain Enzymes, Catalase Activities and Lipid Peroxidation Levels in Lung, Heart and Brain Tissues of Rats.

Cisplatin is one of the most active cytotoxic agents in cancer treatment. To clarify the interaction with mitochondria, we hypothesize that the activities of mitochondrial electron transport chain (ETC) enzymes succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), nucleotide levels, as well as levels of catalase (CAT) enzyme and membrane lipid peroxidation (LPO) can be affected by cisplatin. There was a significant decrease of both SDH and COX activities in the lung, heart, and brain tissues at the 1st day after cisplatin exposure, and the observed decreased levels of adenosine triphosphate (ATP) and adenosine diphosphate (ADP) in comparison with the control could be because of cisplatin-induced mitochondrial dysfunction. The investigations suggested that cisplatin inhibits SDH, COX, and ATP synthase. The higher LPO level in the studied tissues after 1 and 4 days post-exposure to cisplatin compared to control can be inferred to be a result of elevated electron leakage from the ETC, and reactive oxygen species (ROS) can lead to wide-ranging tissue damage such as membrane lipid damage. Consequently, it was observed that capsaicin may have a possible protective effect on ETC impairment caused by cisplatin. The activities of SDH and COX were higher in heart and brain exposed to cisplatin + capsaicin compared to cisplatin groups, while LPO levels were lower. The investigated results in the cisplatin + capsaicin groups suggested that the antioxidant capacity of capsaicin scavenges ROS and prevents membrane destruction.

227 - Neuroprotective efficacy of curcumin in lead (Pb) induced inflammation and cholinergic dysfunction in mice

Curcumin from the rhizomes of Curcuma longa has many pharmacological activities including antioxidant, anti-inflammatory and cancer preventive properties. This study was designed to assess the protective effect of Curcumin against lead induced neurotoxicity. ICR mice were exposed to lead acetate (Pb) in drinking water (1%) with or without orally administrated Curcumin (100 and 200 mg/kgBW) and Vitamine E, the antioxidant positive control, once daily for 38 days. The results exhibited that Curcumin significantly protected against Pb induced learning deficit and memory loss in a concentration dependent manner, indicated by Water maze swimming test and Forced depression test. Pb induced a profound alteration of circulating Tumor necrosis factor-α (TNF-α), alteration inflammatory markers, TNF-α, COX-2 and iNOS, and elevation of oxidative damage, as evidenced by increasing lipid peroxide (malondialdehyde, MDA) levels in blood and brain tissues of mice as found in mice-treated with Vitamine E. The toxic effects of Pb may be mitigated by Curcumin and Vitamine E due to ameliorated inflammatory markers and increased expression of ChAT and AChE proteins lead to increased AChE activity in the brain compared to Pb alone, as shown by Western blot analysis. Taken together, these results suggested that Curcumin significantly alleviates Pb induced neurotoxicity, at least in part, by suppression inflammation, oxidative damage, alteration in neurotransmitter and enzyme expression (AChE and ChAT) with a reduction learning deficit and memory loss.

Effects of Asparagus racemosus Root Extracts on Serum Lipid Profiles, Lipid Peroxidation and Superoxide Dismutase in Ovariectomized Rat

Background: Once rats have been ovariectomized they have a high risk of cardiovascular disease due to changes in the blood cholesterol and lipid profile. Objective: To investigate the effects of Asparagus racemosus (AR) root extract on the serum lipid profiles, lipid peroxidation and antioxidant levels in ovariectomized rats. Methods: Twenty-five, two month-old female Wistar rats were randomly divided into five groups: SH, OVX,OEE, OAAR and OEAR. The daily doses of 500 mg/KgBW of the AR root extracts for five weeks.The levels of serum TG, TC, HDL, LDL, the liver, kidney and uterine tissue lipid peroxidation and SOD levels were determined. Results: Serum TC and LDL showed no significant differences in any groups. Serum TG of the OAAR and OEAR groups were not significantly different. The serum HDL of the OAAR and OEAR groups were significantly lower than the OEE group. The liver MDA levels of the OAAR and OEAR groups were significantly decreased compared with the OVX and OEE groups while the SOD level of the OAAR group was significantly increased. The MDA levels in the kidney and uterine of the treated group showed no significant difference. The SOD levels in the kidney of the treated group were not different but the SOD levels in uterine were significantly decreased. Conclusion: It can be believed that the lipid profiles were maybe regulated via estrogen.The AR extract has low effects on the lipid profiles at this dose and duration of treatment. The capacity of the extracts to decrease the MDA level and increase the SOD level in this study clearly reflected the antioxidant efficiency of these substances.

Protective effect of an arabinogalactan from black soybean against carbon tetrachloride-induced acute liver injury in mice

In vivo hepatoprotective effect of a novel arabinogalactan (AG) from black soybean on carbon tetrachloride (CCl4)-induced acute liver injury was evaluated for the first time. Our results showed that administration of AG could significantly attenuate the increase in the levels of alkaline phosphatase, alanine transaminase and aspartate transaminase in the serum caused by CCl4 treatment. AG treatment not only significantly elevated the levels of antioxidant enzymes including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase; but also increased the levels of non-enzyme antioxidants (glutathione and total antioxidant capacity in liver tissues) when compared with CCl4-induced acute liver injury model. In addition, AG significantly reduced lipid peroxidation levels in liver tissues. The hepatoprotective effect of high-dose of AG was comparable to that of silymarin, which served as a positive standard. This study indicated that AG had a protective effect on CCl4-induced acute liver injury.

Protective effect of Sesbania grandiflora on acetic acid induced ulcerative colitis in mice by inhibition of TNF-α and IL-6

Ethnopharmacological relevanceThe plant Sesbania grandiflora (Linn) belonging to the family Fabaceae is commonly known as sesbania, agathi, and katurai. The plant is accredited for alleviating a spectrum of ailments including inflammation, colitis, diarrhea, dysentery, leprosy, gout, rheumatism, jaundice, bronchitis, convulsion and anxiety. It is also used as antitumour, anthelmintic, and laxatives in Ayurveda and Siddha system of Indian traditional medicine. AimTo reveal protective effect of Sesbania grandiflora in acetic acid induced ulcerative colitis in mice. Materials and methodPolyphenol, flavonoid and flavanone contents of different extracts of S. grandiflora leaves were quantified and correlated with their antioxidant capacity in-vitro (DPPH assay) for identification of potential fraction. In further studies hydroalocholic extract (HASG, 100 and 200 mg/kg) was evaluated for protective effect towards acetic acid induced ulcerative colitis (UC) animals administered with 150 µl of 5% acetic acid once, intrarectally. The colonic mucosal injury was assessed by estimating disease activity index (DAI), which took into account weight loss, stool consistency and occult/gross bleeding. Macroscopic changes like colon length, spleen weights, ulcer area and ulcer index were determined. Haematological parameters like WBC count, RBC count, Hb (g/dL), HCT (%), PLT count and FFA level were determined. Biochemical analysis was carried out for asserting the levels of tissue myeloperoxidase (MPO) accumulation, SOD concentrations, reduced GSH and lipid peroxidation in UC induced and treated animals. The cardinal inflammatory biomarkers like nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin (IL-6) were determined. Histopathological investigation was carried out and scores were calculated. ResultsHASG showed presence of highly polymerized polyphenols and flavonoids amongst other extracts of S. grandiflora, which is correlated to its rich antioxidant potential (IC50 =19.21). HPLC fingerprinting quantifies the presence of quercetin in concentration of 81.7 µg/mg of HASG. HASG (200 mg/kg) and Prednisolone (2 mg/kg) significantly reduced DAI and macroscopic scores. The haematological changes in experimental animals were restored upon treatment with HASG and Prednisolone. HASG showed potent antioxidant activity (In-vivo) by restoring the levels of SOD, GSH, MPO, MDA and NO. HASG was found to inhibit FFA levels, which may indicate inhibition of TLR4 receptor mediated inflammation. The levels of serological biomarkers like TNF-α and IL-6 were found to be suppressed. Histopathological investigation reveals decrease signs of ulceration, necrosis, cellular infiltration, hyperaemia in HASG treated animals. The results of HASG (200 mg/kg) were found to be comparable with Prednisolone (2 mg/kg) significantly. ConclusionThe protective action of HASG against acetic acid induced UC is attributed to the antioxidant like action (In-vitro and In-vivo) of highly polymerized polyphenols and flavonoids especially quercetin. Also HASG was found to reduce the levels of TNF-α and IL-6, thereby suppressing their inflammatory response in UC.

Role of Ascorbic Acid in Ameliorating Testicular Tissue Damage Induced by Testicular Torsion and Detorsion: An Animal Model Study

Introduction: Testicular torsion-detorsion induced tissue damage, in neonates or adolescences may hamper fertility potential in their future life. Ascorbic acid being a water-soluble antioxidant has a number of antioxidant properties and is the most important antioxidant in human plasma. Aim: To evaluate the potential role of ascorbic acid in ameliorating testicular tissue damage induced by testicular torsion and detorsion in wistar rats. Materials and Methods: Forty male albino wistar rats were randomly divided into four groups. Group I served as normal control while Group II underwent Sham operation. Animals of Group III underwent testicular torsion and detorsion without any treatment while animals of Group IV were pretreated with ascorbic acid for 30 days, followed by 3 hours of testicular torsion and one hour detorsion. In the animals that underwent testicular torsion the testis appeared devitalised and a mild testicular oedema was observed. On detorsion, there was a slight further increase in oedema beside reactive hyperaemia. Other than this no complications were seen in the rats during the observation period. All the animals were sacrificed 1 hour after the experimental procedure, and testicular tissue sample was collected and evaluated for Seminiferous Tubular Diameter (STD), Seminiferous Epithelial Height (SEH), tubular necrosis, tissue lipid peroxidation, tissue glutathione and superoxide dismutase levels. Statistical analysis was done using SPSS package version II. The analysis of multiple group variation was done by ANOVA. Intergroup comparison was done by post-hoc (LSD) test. Results: Animals of Group III showed a decrease in STD and SHE compared to Groups I and II. A four-fold increase in lipid peroxidation was observed in these animals (Group III). Superoxide dismutase and tissue glutathione levels were considerably reduced in these animals. Whereas, animals of Group IV showed merely 25% of seminiferous tubular necrosis with no significant damage in their STD and SEH. A significant reduction in lipid peroxidation was also observed in these animals with antioxidants showing near to normal value comparing to their untreated controls. C onclusion: Results of the present study display that pretreatment with ascorbic acid offers salvaging effect on the testicular torsion-detorsion induced injury in rats.

Impact of co-exposure of aldrin and titanium dioxide nanoparticles at biochemical and molecular levels in Zebrafish

Aldrin (ALD), a persistent-organic-pollutant (POP), an organochlorine-cyclodiene-pesticide is highly toxic in nature. Titanium dioxide nanoparticles (TNP) are widely used for various industrial applications. Despite the remarkable research on pesticide toxicity, the work with impact of nanoparticles on POP has been dealt with marginally. Chemicals co-exist in the environment and exhibit interactive effects. An investigation was carried out to evaluate the individual and combined effects of ALD (6 ppm) and TNP (60 ppm) exposure at sub-lethal concentration for 24 h in zebrafish. Significant reversal of lipid peroxidation level in liver and brain tissues and restoration in enhanced catalase activity in all examined tissues were observed in combined group. For other parameters, combined exposure of ALD and TNP does not show significant reversal action on ALD toxicity. Further studies are inline to understand combined effects of both to achieve significant reversal of ALD toxicity by TNP nanoparticles with threshold concentration of aldrin.

A Mini-Review on the Effect of Docosahexaenoic Acid (DHA) on Cerulein-Induced and Hypertriglyceridemic Acute Pancreatitis.

Acute pancreatitis refers to the sudden inflammation of the pancreas. It is associated with premature activation and release of digestive enzymes into the pancreatic interstitium and systemic circulation, resulting in pancreatic tissue autodigestion and multiple organ dysfunction, as well as with increased cytokine production, ultimately leading to deleterious local and systemic effects. Although mechanisms involved in pathogenesis of acute pancreatitis have not been completely elucidated, oxidative stress is regarded as a major risk factor. In human acute pancreatitis, lipid peroxide levels in pancreatic tissues increase. Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid (C22:6n-3), exerts anti-inflammatory and antioxidant effects on various cells. Previous studies have shown that DHA activates peroxisome proliferator-activated receptor-γ and induces catalase, which inhibits oxidative stress-mediated inflammatory signaling required for cytokine expression in experimental acute pancreatitis using cerulein. Cerulein, a cholecystokinin analog, induces intra-acinar activation of trypsinogen in the pancreas, which results in human acute pancreatitis-like symptoms. Therefore, DHA supplementation may be beneficial for preventing or inhibiting acute pancreatitis development. Since DHA reduces serum triglyceride levels, addition of DHA to lipid-lowering drugs like statins has been investigated to reduce hypertriglyceridemic acute pancreatitis. However, high DHA concentrations increase cytosolic Ca2+, which activates protein kinase C and may induce hyperlipidemic acute pancreatitis. In this review, effect of DHA on cerulein-induced and hypertriglyceridemic acute pancreatitis has been discussed. The relation of high concentration of DHA to hyperlipidemic acute pancreatitis has been included.

Pre and Post-Treatment Effects: Estimation of Serum Testosterone and Lipid Peroxidation Levels on Moringa olifera Extract Induced Cadmium Exposed Rats

Background: Cadmium (Cd), is a toxic metal which affects various organs including testis. It produces oxidative stress leading to male infertility. Moringa tree, is a natural plant with a great therapeutic value and hence it is found to be effective both in prevention and treatment of various conditions including reducing toxicity of hazardous materials. The aim of the present study was to examine the effects of Pre-and Post-treatment with Moringa oliefera leaf extract (MoE) on testis in cadmium exposed rats. Materials and Methods: The present study was conducted at the Department of Physiology, Kasturba Medical College (KMC), Mangalore, Manipal University (MU), Karnataka, India, between (2011-2013). This prospective study consisted a total of 30 rats. These were divided into 5 groups with group I being the control. Data were presented as mean ±SD. student’s t test was used as statistical tool, p<0.05 considered statistically significant. Group IV and V were pre-and post-MoE treated groups respectively. Serum testosterone and tissue lipid peroxidation levels were estimated. Results: Treatment with MoE prior and after administration of cadmium, respectively showed an increase significantly in the testosterone levels and a decrease in the tissue lipid peroxidation as compared to the group treated with cadmium. However, the pre-treatment showed better results in combatting the toxic effects of cadmium. Conclusion: This study shows that Moringa olifera leaf extract altered the testosterone and tissue lipid peroxidation levels. Also, pre-treatment showed better outcome.

Oxidative Stress in the Visceral Fat Is Elevated in Postmenopausal Women with Gynecologic Cancer.

This study aimed to evaluate differences in oxidative stress of visceral fat between premenopausal and postmenopausal women and clarify the antioxidant effect of estrogen on adipocytes. Abdominal subcutaneous and omental visceral adipose tissues were obtained from 38 patients who underwent gynecological surgery. We measured the sizes of the adipocytes and evaluated the lipid peroxidation levels in the adipose tissues. We investigated whether estrogen inhibited the intracellular reactive oxygen species (ROS) production that was induced by hydrogen peroxide (H2O2) in 3T3-L1 adipocytes. The visceral adipocytes were 1162.4 μm2 and 1881.9 μm2 in premenopausal and postmenopausal women, respectively; hence they were significantly larger in the latter (p < 0.05). The lipid peroxidation levels were 46.7 nmoL/mg protein in premenopausal women and 99.6 nmoL/mg protein in postmenopausal women; hence the lipid peroxidation levels were significantly higher in the latter (p < 0.05). Estradiol (E2) significantly reduced the intracellular ROS levels that were induced by H2O2 in 3T3-L1 adipocytes (p < 0.01). We determined that E2 significantly increased the expression of nuclear factor erythroid 2-related factor 2 (NRF2)-dependent antioxidant genes, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and the glutamate-cysteine ligase (GCL) modifier subunit genes, in 3T3-L1 adipocytes (p < 0.01). Oxidative stress in the visceral fat is higher in postmenopausal women. The expression of the antioxidant genes HO-1, NQO1, and GCL was upregulated by estrogen in 3T3-L1 adipocytes. Hence, estrogen may act as an antioxidant in the adipose tissues of premenopausal women.