Levels Of Tannins Research Articles

Effects of cooking on antinutrients and antioxidant properties of different accessions of winged bean (Psophocarpus tetragonolobus)

Asides from its robust nutritional profile, Psophocarpus tetragonolobus also possesses other health-promoting attributes. Hence, this study was designed to investigate the antinutrient content and antioxidant property of five different varieties of raw and cooked P. tetragonolobus. The varieties studied included, the indigenous variety (designated local) and four improved varieties (designated: T11, T31, T125, T154). Antinutrients like phenolic, tannin, glycoside and phytate concentrations were determined using standard methods while 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was carried out to ascertain the antioxidant activity. Phytate, cyanogenic glycosides and tannin levels were significantly (p<0.05) reduced due to cooking in T11 variety. In T125 variety, tannin was significantly lower in the cooked sample compared to the raw sample. However, the phenolic content in the T125 variety increased significantly (p<0.05) due to cooking. There was no significant difference in antioxidant activity between the raw and cooked samples across the five varieties. However, the improved varieties showed higher antioxidant activity compared to the local. Based on these results, the cooking process reduced the phytate, cyanogenic glycosides and tannin levels in the T11 variety while only the tannin level in T125 variety was significantly reduced. However, the antioxidant activity of P. tetragonolobus was not significantly impacted by cooking.

Test of Phytocemical Levels of Bidara (Ziziphus spina-christi L.) Potential as Medicinal Plants

Arabic bidara or Ziziphus spina-christi L. is a plant that has potential in the traditiobal medicine industry. Based on research reports on bidara leves (Ziziphus spina-christi L.) can be useful as antioxidants, anti-inflammatory, antimicrobial, antifungal and prevent tumors. Bidara leaves (Ziziphus spina-christi L.) contain secondary metabolites that have many health benefits, secondary metabolites include flavonoids, alkaloid, saponins, steroids and tannins. The pupose of this study: To determine the levels of phytochemicals in the leavels of the bidara Arabic plants (Ziziphus spina-christi L.) as a medicinal plant. According to Mulyatiningsih (2011) this research uses a quantitative descriptive method. Based on the results of the study, the levels of the bidara leaf (Ziziphus spina-christi L.) produced varying levels of secondary metabolites, namely alkaloid, flavonoids, saponins, steroids and tannins. The average levels of secondary metabolites, including flavonoids, produced levels of 3.030%, alkaloids produced levels of 23.537% saponins produced levels of 5.5307, tannis produced levels of 0.0933% and steroid produced levels of 3.494%. of the five compounds, the highest compounds that have the potential as medicinal plants are alkaloid compounds that produce levels as much as 23.537%, and the lowest compounds, namely tannins, produce levels of 0.0933%. The hope in the future from the compounds that have been studied is that they are used as herbal medicines by the community and as references for for other researchers.

Evaluating the potential for utilising migratory locust powder (Locusta migratoria) as an alternative protein source in peanut-based ready-to-use therapeutic foods.

This study aimed at exploring the possibility of substituting powdered milk (PM) with migratory locust powder (MLP) as a cheap and sustainable alternative protein source in peanut-based ready-to-use therapeutic foods used in treatment of malnutrition. The migratory locust is a wide spread and underutilised high protein edible insect. Peanut-based ready-to-use therapeutic foods (RUTFs) were formulated according to the MSF/UNICEF 2013 nutritional standards. Milk powder was substituted with MLP at 5-30% levels. RUTF containing 30% milk powder was used as the control. Nutritional, antioxidant and antinutrients composition of the insect-enriched RUTFs were compared with RUTF containing milk powder only and a standard. The protein content of the insect-enriched RUTFs ranged from 19.58-26.38% exceeding the MSF/UNICEF minimum standard for protein. RUTF with 10% PM and 20% MLP had the highest (p < 0.05) mineral contents. All formulations had more thiamine (vitamin B1) than the recommended level of 0.5 mg/100 g. Niacin (Vitamin B3) was the most abundant vitamin in the formulations with considerable amounts of vitamin D and E. All MLP-enriched RUTFs met the FAO 2011 standard requirements for amino acids for children aged six months to three years. Therapeutic food with 30% MLP had the highest radical scavenging ability against DPPH, and iron chelating activity. Levels of phytate and tannin were below the tolerable limits. Therefore, MLP can be used as a low-cost substitute for milk powder in producing ready-to-use therapeutic foods.

Optimization of Microwave Assisted Extraction Conditions to Improve Phenolic Content and In Vitro Antioxidant and Anti-Microbial Activity in Quercus cerris Bark Extracts.

The species of the Quercus genus, including Quercus cerris L., are widely used and their wood represents a valuable material utilized for various purposes. The intense industrial processing of wood results in a considerable amount of poorly-used natural by-products, such as bark, and a loss of potentially useful raw materials. Thus, the aim of our study was to evaluate the phytochemical characteristics and potential biological activities of Quercus cerris bark extracts obtained by optimizing the parameters of microwave assisted extraction (MAE). The optimum conditions for MAE were determined using a design of experiments (DoE) model, which set the experimental variables (irradiation time and microwave power) and their values. Aqueous and hydroalcoholic extractions were performed and the optimum parameters of extraction were determined for both solvents. The total polyphenolic and tannin contents were determined. The biological activities representative of antioxidant capacity were determined using two free radical scavenging methods, the DPPH and ABTS methods, and the antibacterial activity was assessed with the microdilution method. The results showed different optimal extraction conditions for aqueous (30 min at 850 W) and hydroalcoholic (18 min at 650 W) extracts. A higher yield of total polyphenols was observed in the hydroalcoholic bark extract (403.73 ± 7.35 mg gallic acid equivalents/g dried weight); however a lower level of tannins was registered in comparison to the aqueous extract. In addition, both extracts exerted high antioxidant activities, with the aqueous extracts having a stronger inhibitory effect against the DPPH radical. Moreover, the extracts exhibited antibacterial activity against the tested bacterial strains, especially against the Gram-positive strains and Klebsiella pneumoniae, with the hydroalcoholic extracts being more efficient overall. To conclude, the optimized MAE was an efficient method to extract phytochemical compounds with potential biological effects from Quercus cerris bark.

Evaluation of in vitro antioxidant potential in different parts of Syzygium caryophyllatum (L.) Alston

Studies on oxidative stress and its consequences on human health have gained a lot of attention in recent years. Many diseases are caused by an imbalance between pro-oxidant production and neutralization. Antioxidant principles obtained from plant resources have a wide range of impacts and provide a lot of potential for rectifying the imbalance. These natural antioxidants are recognized to aid in the treatment of variety of human illnesses by inhibiting the formation of free radicals. In the present studies, in vitro antioxidant studies were conducted using methanolic extract of plant parts of Syzygium caryophyllatum (L.) Alston (Myrtaceae). The total polyphenols, flavonoids and tannins were determined after the extraction. DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), NO (nitic oxide), FRAP (ferric reducing antioxidant power) and reducing power assays were done to evaluate antioxidant activities. Maximum levels of polyphenols (431.85±2.34 mg of GAE/g of extract), total flavonoids (201.62 ±3.39 mg of QE/g of extract) and tannins (355.32±1.77 mg of TAE/g of extract) in the bark was recorded. The bark extract showed more scavenging activity with IC50 value for DPPH, ABTS and nitric oxide were found to be 6.20±0.001 μg/ml, 13.785±0.196 μg/ml and 79.855±0.003 μg/ml, respectively. When compared to the standard ascorbic acid that is commonly used to measure antioxidant capacity, the leaf, fruit, seed and bark extracts showed remarkable antioxidant activity. The result of this study are encouraging, suggesting that the bark of S. caryophyllatum can be exploited as a potent antioxidant in a variety of formulations for the treatment of disorders resulting from excessive biological oxidative stress.

Development of functional cookies with Cerrado fruits and residues: sensory analysis, nutrients, and bioactive compounds

In this study, we determined the nutritional and functional characteristics of cookies supplemented with chichá almond, gurguéia nut, and soursop residue using two developed cookie formulations and one standard formulation. Acceptance and purchase intention were evaluated. As the chichá almond 6 and gurguéia nut 2 formulations were sensorially viable, descriptive quantitative analysis, centesimal composition and caloric value, mineral levels, microbiological quality, bioactive compound levels, and antioxidant capacity were evaluated. In the hedonic scale test of the standard cookie, the chichá almond-6 and gurguéia nut-2 cookies obtained scores above 7 (“I liked it”) from 86.18%, 96.35%, and 93% of the evaluators, and 70.79%, 94.36%, and 83.2% of them said they would purchase the product, showing a statistically significant difference between formulations. The chichá almond 6 cookie presented higher ash, protein, lipid, copper, phosphorus, magnesium, potassium, selenium, and zinc levels, and lower carbohydrate levels and total energy values, as well as a higher total phenolic compound, total flavonoid, and condensed tannin levels. The gurguéia nut 2 cookie showed higher vitamin C and antioxidant activity. The use of residue and Cerrado fruit flour proved to be viable in cookie production, conveying sensorially desirable, nutritional, and functional characteristics.

Sentul Fruit (Sandoricum koetjape) Peel as Anti-Inflammation for Gingivitis after Scaling

Various herbs are used as analgesic, anti-inflammatory, anti-bacterial, anti-fungal, expectorant, anti-plaque and odorant. Sentul is an edible fruit and is also used in traditional medicinal herbs which can treat diarrhea, relieve fever, and as an anthelmintic. Sentul bark methanol extract can inhibit the growth of fungus Candida albican by 39.65%. In addition, the ethyl acetate extract of the sentul leaves also has anti-bacterial activity. The aim of this study is to determine the effect of fractionation with different types of solvents on the phytochemical compounds of Sentul fruit peel in Bali province. This research is an experimental study in a laboratory with qualitative and quantitative analysis models of chemical compounds. This research was carried out from March to August 2021. The research location was carried out in the laboratory of the Faculty of Food Technology, Udayana University. Sample criteria was old Sentul peel, about 30 kilograms. Data was collected based on the results of examinations from the Laboratory of the Faculty of Food Technology, Udayana University which subsequently analyzed qualitatively and descriptively. From several phytochemical compounds, flavonoids, saponins and tannins are aromatic hydroxyl groups that act as antibacterial. Therefore, seen from the highest levels of flavonoids, saponins and tannins, aqua fraction of Sentul ethanol extract is the best treatment with flavanoid levels of 11476.16 mg/100g QE, tannins 88.605 mg/g and saponins 6.862 mg/g.

Pengaruh Eksitasi Gelombang Iradiasi Ultrasonik Terhadap Kadar Tanin pada Maserasi Biji Pinang Wangi

Nagari Sikucua, Kampung Dalam Subdistrict, Padang Pariaman produces nearly 800 tons of fragrant areca nut every year which is sent to Jambi to meet export needs. Areca nut contains tannins, substances that have many benefits, some of which are anti-inflammatory, corrosion inhibitors, antioxidants. This study aims to obtain tannins in areca nut extract through a kinetic maceration process with pre-treatment with ultrasonic irradiation wave. The dried areca nut was ground and sieved with various sizes of 20, 40 and 70 mesh and then extracted with ethanol as solvent. The concentration of ethanol used consisted of 35%, 50%, 65% and 80%. Ultrasonic irradiation waves were given for the initial 15 minutes of maceration and then continued with kinetic maceration for up to 4 hours in a water bath at 45°C. The extraction results were concentrated in a rotary evaporator and then tested for tannin levels using Follin Denish reagent in the standard series of tannic acid as a comparison with spectrophotometer UV-Vis. The highest yield of tannin content 11.10% was obtained in the 4-hour maceration process with pre-treatment ultrasonic irradiation at concentration of 80% ethanol with a size of 40 mesh.

Penetapan Kadar Tanin Dari Bunga Telang (Clitoria ternatea L.) Secara Permanganometri

&#x0D; &#x0D; &#x0D; &#x0D; Telang flowers can be found thriving in watery areas in Indonesia, their purple flowers bloom throughout the year. Telang flowers have been studied to contain phenolic chemicals, flavonoids, anthocyanins, flavonol glycosides, kaempferol glycosides, quercetin glycosides, myristetin glycosides, terpenoids, flavonoids, tannins and steroids. Tannins are plant polyphenols that function to bind and precipitate proteins. In the world of tannin medicine serves to treat diarrhea, stop bleeding, and treat hemorrhoids. There are two main types of tannins namely condensed tannins and hydrolyzed tannins.&#x0D; The purpose of this study was to determine the tannin content in the extract of telang (Clitoria ternatea L.) flowers using the permanganometric method. Determination of tannin levels by permanganometric method. Permanganometry is a titration method using potassium permanganate, which is a strong oxidizing agent as a titrant. This property of KMnO4 is known as an auto-indicator.&#x0D; The result of the determination of the tannin content of the telang flower (Clitoria ternatea L.) by permanganometry was 13.86% b/b.&#x0D; &#x0D; &#x0D; &#x0D; &#x0D;

Influence of UV-B treatment on the accumulation of free phenols and tannins in the legumes of Abrus precatorius L. and Vigna mungo (L.) Hepper

BackgroundUltraviolet B radiation with a wavelength from 280 to 320 nm (UV-B) is a prime abiotic factor and environmental stress that can trigger the accumulation/increase of secondary metabolites like phenolic compounds that are unique sources of pharmaceuticals. The Fabaceae family legume seeds or grains contain different kinds of bioactive compounds chiefly classified under phenolic compounds whose potential benefits to human health need to be evaluated for their orderly utilization. This paper aims to study the influence of UV-B radiation on the increase of total free phenols and tannins in the seeds of a wild legume and a crop legume (Abrus precatorius L. and Vigna mungo (L.) Hepper) as a process of fortifying the levels of these bioactive secondary metabolites. MethodThe flour of raw seeds, UV-B exposed raw seeds, soaked seeds, and UV-B exposed soaked seeds of Abrus precatorius and Vigna mungo was used to study the influence of UV-B on the accumulation/increase of total free phenols and tannins in the seeds selected for this study. ResultsBoth raw (dry) and overnight soaked seeds showed increased levels of total free phenols and tannins in response to UV-B exposure. ConclusionIt is concluded that UV-B radiation-related stress (an external stimulus) will have a major influence on the accumulation/increase of secondary metabolites in higher plants.

In-Vitro Study of Antimicrobial and Antiproliferative activity of Cyanogenic glycoside extracted from Bamboo shoot of Bambusa arundinacea

Bambusa arundinacea is a common bamboo species found in Andaman and Nicobar islands. There are over 1200 species of bamboo belonging to 75 genera which has been documented worldwide. Bamboo plants especially the leaves and shoots are known to have cyanogenic glycosides like taxiphyllin and amygdalin apart from vitamin C and other components. Immature bamboo shoots are consumed as vegetable in several asian countries. In the present study the crude extract of Bambusa arundinacea in various solvents was performed. Further preliminary phytochemical screening of the extract was done. Based the results of phytochemical screening, methanol extract was selected for further study. HPLC analysis was done confirm the presence of Amygdalin. GC-MS Analysis of methanol extract of B.arundinacea shows the presence of 40 compounds. The major compounds found in the methanol extracts based on the retension time were beta.-Sitosterol; 2-Methyl-7-phenylindole, 1-(Methylamino)anthraquinone, (R)-(-)-14-Methyl-8-hexadecyn-1-ol and 2-Ethylbutyric acid, nonadecyl ester. Antimicrobial activity of the extracts were tested using two Gram positive bacteria namely S. aureus and pneumonia and two Gram negative bacteria viz. Escherichia coli and Streptococcus mutant. The zone of inhibition was maximum in acetone and ethylacetate extracts while it was minimum for methanol extract. This might be due to the presence of taxiphyllin in the acetone and ethylacetate extract which are relatively non-polar as compared to methanol. To determine whether the inhibition of cell proliferation by methanolic extract from bamboo shoot was due to the induction of apoptosis, PC3 cell exposed to methanol extract were stained with acridine orange/ethidium bromide. The results show that at dose of 150 μg/mL, methanolic extract showed increased percentages of early apoptotic cells. Based on the results of the study, methanol extract of Bambusa arundinacea is a rich source of secondary metabolites, with presence of high levels of flavanoids and tannins. Presence of amygdalin in methanol extract is confirmed by HPLC analysis. Though the extract has a comparatively low antimicrobial activity yet the high anti-apoptotic activity of the extract coupled with a rich antioxidant ativity makes it an ideal candidate as a source of drug for cancer therapy.

Deficit irrigation and leaf removal modulate anthocyanin and proanthocyanidin repartitioning of Cabernet Sauvignon (Vitis vinifera L.) grape and resulting wine profile.

In monsoonal climates, grape anthocyanin and proanthocyanidin (PA) accumulations are unsatisfactory for producing optimal wine. Agronomical practices are often considered to be effective means for regulating fruit components. However, there is a lack of quantitative information on the effects of deficit irrigation (DI), basal leaf removal (LR) or their combination of deficit irrigation and leaf removal (DILR) on the characteristics of anthocyanin and PA compositions and their implications on the resulting wine quality. In this study, the dynamics of grape anthocyanin and PA accumulation were investigated in DI, LR and DILR during grape ripening, and the resulting wine profile was assessed. The contents of reducing sugar and total anthocyanins in Cabernet Sauvignon berries were significantly increased by DI, LR and DILR, while titratable acidity, total flavan-3-ols and tannins levels were generally decreased. Notably, the levels of 3'5'-substituted anthocyanins, such as malvidin and its derivatives significantly increased, and 3'-substituted anthocyanins decreased in both grape and wine under DI and DILR strategies. Skin PAs were sensitive to water deficits, whereas they were insensitive to LR. In resulting wine, PAs content and the proportion of 3'-hydroxylated PAs, such as (+)-catechin, (-)-epicatechin and (-)-epicatechin-3-O-gallate units were significantly decreased under DI and DILR, while molecular mass and the proportion of 3'5'-hydroxylated units of PAs were increased in response to DILR. The DILR was the most favorable for the repartitioning of anthocyanin and PA metabolites, and promoted the accumulation of tri-substituted forms contributing a higher color intensity, mouthfeel persistence, structure, and astringency of wine. This information provides an important strategy for modulating the anthocyanin and PA compositions by agricultural practices and achieving the desired quality of grapes and wines in monsoonal climates. © 2021 Society of Chemical Industry.

Bioactive Compounds of Fruit Parts of Three Eugenia uniflora Biotypes in Four Ripening Stages.

Variability of secondary metabolites in edible (peel and pulp) and inedible (seeds) parts of three pitanga varieties, red, red-orange and purple, was investigated during the maturation process. Hydrolysable tannins, anthocyanins, and flavonoids were quantified by HPLC/DAD and carotenoids by absorbance. Peel/pulp showed greater complexity of constituents (carotenoids, anthocyanins, flavonoids, and hydrolysable tannins), while only tannins were identified in seeds, but in quantities of 10 to 100 times greater. The red-orange variety showed the highest levels of phenolic compounds in seeds and peel/pulp, except anthocyanins. The analysis of the principal response curves showed that the pitanga biotype has greater influence on metabolite variation than ripening stages. During peel/pulp maturation, a reduction in the levels of flavonoids and tannins contrasted with an increase in carotenoids and cyanidin-3-O-glucoside in all varieties, whereas in the seeds oenothein B, the major tannin, increased up to 1.32 g/100 g fresh weight. Such marked differences between fruit parts demonstrate that the seeds in stages E3 and E4 are a source of hydrolysable tannins, compounds known for their antitumor activity, while peel/pulp of all varieties in the ripe stage provide natural antioxidants, such as carotenoids and flavonoids. Lastly, the purple biotype can be a rich source of the cyanidin-3-O-glucoside pigment a potent bioactive compound.

Antioxidant and Starch-Hydrolyzing Enzymes Inhibitory Properties of Striga-Resistant Yellow-Orange Maize Hybrids.

Most of the health benefits derived from cereals are attributed to their bioactive compounds. This study evaluated the levels of the bioactive compounds, and the antioxidant and starch-hydrolyzing enzymes inhibitory properties of six pipeline Striga-resistant yellow-orange maize hybrids (coded AS1828-1, 4, 6, 8, 9, 11) in vitro. The maize hybrids were grown at the International Institute of Tropical Agriculture (IITA), Nigeria. The bioactive compounds (total phenolics, tannins, flavonoids, and phytate) levels, antioxidant (DPPH• and ABTS•+ scavenging capacity and reducing power) and starch-hydrolyzing enzymes (α-amylase and α-glucosidase) inhibitory activities of the maize hybrids were determined by spectrophotometry. At the same time, carotenoids were quantified using a reverse-phase HPLC system. The ranges of the bioactive compounds were: 11.25–14.14 mg GAE/g (total phenolics), 3.62–4.67 mg QE/g (total flavonoids), 3.63–6.29 mg/g (tannins), 3.66–4.31% (phytate), 8.92–12.11 µg/g (total xanthophylls), 2.42–2.89 µg/g (total β-carotene), and 3.17–3.77 µg/g (total provitamin A carotenoids). Extracts of the maize hybrids scavenged DPPH• (SC50: 9.07–26.35 mg/mL) and ABTS•+ (2.65–7.68 TEAC mmol/g), reduced Fe3+ to Fe2+ (0.25 ± 0.64–0.43 ± 0.01 mg GAE/g), and inhibited α-amylase and α-glucosidase, with IC50 ranges of 26.28–52.55 mg/mL and 47.72–63.98 mg/mL, respectively. Among the six clones of the maize hybrids, AS1828-9 had the highest (p < 0.05) levels of tannins and phytate and the strongest antioxidant and starch-hydrolyzing enzymes inhibitory activities. Significant correlations were observed between total phenolics and the following: ABTS•+ (p < 0.01, r = 0.757), DPPH• SC50 (p < 0.01, r = −0.867), reducing power (p < 0.05, r = 0.633), α-amylase IC50 (p < 0.01, r = −0.836) and α-glucosidase IC50 (p < 0.05, r = −0.582). Hence, the Striga-resistant yellow-orange maize hybrids (especially AS1828-9) may be beneficial for alleviating oxidative stress and postprandial hyperglycemia.

Evaluation of the phytochemical content, antimicrobial and antioxidant activity of Cocos nucifera liquid smoke, Garcinia mangostana pericarp, Syzygium aromaticum leaf, and Phyllanthus niruri L. extracts.

Background and Aim:Many plants contain bioactive substances with antibacterial and antifungal properties. The aim of this study was to evaluate the antibacterial and antifungal activity of Cocos nucifera shell liquid smoke (CSL), clove leaf extract (CLE), and mangosteen pericarp extract (MPE) alone and in combination against Escherichia coli and Candida utilis. The antioxidant activity, phenol, saponin, and tannin of CSL, CLE, MPE, and Phyllanthus niruri L. extract were also measured.Materials and Methods:The agar well-diffusion method was used to determine the antimicrobial and antifungal activities of CSL, methanolic MPE, and CLE and their combination CSL+MPE+CLE (COMBI) on bacteria E. coli and fungus (C. utilis). Antioxidant activity was measured by the diphenylpicrylhydrazyl method. Total phenol and total tannin were measured by the Folin–Ciocalteu method and total saponin was measured by the vanillin-sulphate method.Results:The results indicated that phenolic and tannin levels were greater in MPE than in CLE, whereas the saponin content was higher in CLE compared with MPE. Undiluted (100%) MPE exhibited lower antibacterial activity (p<0.05) than chloramphenicol against E. coli, however, undiluted CLE and COMBI showed similar activity compared with chloramphenicol against E. coli. COMBI caused significantly (p<0.05) higher inhibition compared with virginiamycin against E. coli. CSL, MPE, and COMBI exhibited significantly lower antifungal activity (p<0.05) than that of ketoconazole against C. utilis. In contrast, CLE showed improved antifungal activity (p<0.05) compared with ketoconazole.Conclusion:Cocos nucifera liquid smoke, Garcinia mangostana pericarp extract, and Syzygium aromaticum leaf extract, either alone or in combination, have the potential to be used as antibacterial and antifungal agents.

Comparison of Nutritional and Anti-Nutritional Qualities of Grewia forbesii Hav. Ex Mast and Grewia bicolor Juss Fruits from Kitapilimwa Forest Reserve in Iringa District

The Grewia forbesii and Grewia bicolor fruits were collected from Kitapilimwa Forest Reserve and were analysed for proximate, nutrients and anti-nutrients compositions. Results showed low moisture contents in G. forbesii and G. bicolor amounting to 14.14 and 13.98 percent, respectively. Crude fibre percent was slightly higher in G. bicolor (33.15%) than G. forbesii (31.95%). Lipid content was slightly higher in G. bicolor (1.37%) compared to G. forbesii (1.28%). The crude protein content of G. forbesii (7.44%) was slightly higher than G. bicolor (7.00%). Total carbohydrate content of G. forbesii (68.9%) was higher than that of G. bicolor (70%). Mineral analysis indicated substantial amounts of potassium 898 mg/100 g in G. forbesii and 879 mg/100 g in G. bicolor. Calcium content was high in G. forbesii and G. bicolor amounting to 453 mg/100 g and 582 mg/100 g, respectively. Iron was high in both species (20.73–24.45 mg/100 g). Manganese, copper and zinc were substantially low. The results revealed low levels of tannin (&lt; 1.7%) and phylates (&lt; 0.3%). The presence of substantial amounts of nutrients and low anti-nutrients revealed that the studied species may be potential sources of nutritional food.&#x0D; Keywords: Proximate analysis, mineral nutrients, anti-nutritional qualities, Grewia species, Kitapilimwa Forest Reserve

Evaluation of Physicochemical, Functional and Sensory Properties of Carob Pulp Beverage (Ceratonia Siliqua L)

In Morocco, carob tree (Ceratonia siliqua L.) is of considerable socio-economic importance, helping to improve the income of rural populations and to develop the economy of mountain areas. However, the primary use of the carob’s pulp is generally limited in Morocco to animal feed. As a result, the current research suggests a novel pulp-based product designed for human consumption. The main purpose of this work is to characterize a new formulated carob beverage and assess its physicochemical, biochemical, and sensory quality. Two formulas of carob beverages were prepared with two different sugar concentrations (formula A:5 g/100 mL sugar; formula B: 10 g/100 mL sugar). Measurements of pH, titrable acidity, and colour were performed to assess the physicochemical parameters. Total polyphenols and tannins contents were determined as important functional and biochemical parameters. Regarding sensory analysis, preference test, triangle test, hedonic test, and descriptive test, were evaluated. The colour of the two formulations was dark red orange, as determined by chromameter method. For formulae A and B, the titrable acidity was (1.450±0.025) g Citric Acid Equivalent (CAE) /L and (1.450±0.031) CAE g/L, respectively, and the Brix was (11.67±0.22) °Bx and (15.33±0.22) °Bx, respectively. The polyphenols content was (98.8±0.0019) mg Gallic Acid Equivalent (GAE) /100 mL for formula A and (97.7000±0.0017) mg GAE/100 mL for formula B. The tannin level was (24.500± 0.027) mg Catechin (CE)/100 mL and (25.400±0.017) mg CE/100 mL for the formula A and formula B samples, respectively. The sensory analysis tests revealed a distinct difference between the two formulae. In fact, the formula B was highly preferred compared to formula A, also the most prominent features in the drink were colour, smell, flavour, and sweetness.

Seed traits and rodent community interact to determine seed fate: evidence from both enclosure and field experiments.

Animal-mediated seed dispersal is an important ecological process in which a strong mutualism between animals and plants can arise. However, few studies have examined how a community of potential seed dispersers interacts with sympatric seed trees. We employed a series of experiments in the Qinling Mountains in both semi-natural enclosure and the field to assess the interactions among 3 sympatric rodent species and 3 Fagaceae tree seeds. Seed traits all showed similar tannin levels but markedly different physical traits and nutritional contents. We found that seeds with heavy weight, thick coat, and high nutritional contents were less likely to be eaten in situ but more often to be eaten after dispersal or hoarded by rodents. These results support both the handling time hypothesis and the high nutrition hypothesis. Surprisingly, we also found that rodents, maybe, preferred to consume seeds with low levels of crude fiber in situ, and to harvest and hoard those with high levels of crude fiber for later consumption. The sympatric rodent species, Cansumys canus, the largest rodent in our study, harvested and hoarded more Quercus variabilis seeds with high physical and nutritional traits, while Apodemus draco, the smallest rodent, harvested more Q. serrata seeds with low physical and nutritional traits, and Niviventer confucianus harvested and hoarded more Q. aliena seeds with medium physical and nutritional traits. Our study demonstrates that different seed traits play different roles in influencing the seed fate and the shaping of mutualism and predation interactions within a community of rodent species.

Lentil and Fava Bean With Contrasting Germination Kinetics: A Focus on Digestion of Proteins and Bioactivity of Resistant Peptides.

Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure, and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero-tannin lentil [GZL], beluga lentil [BL], and dehulled red lentil [DL]; and zero tannin/low vicine–convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides, and free amino acids (FAAs). In vitro antihypertensive and antioxidant activities of digests were investigated, and the peptidomic characterization [high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS)] and identification of bioactive fragments in intestinal digests were performed. Regardless of the seed type, germination increased protease activity and reduced the levels of phytic acid, trypsin inhibitors, and tannins (only in BL). A significant proteolysis of the 7S and 11S globulins and a concomitant increase of peptides and FAAs were observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAAs and peptides than in dry seeds, with changes being more evident for DL, associated with a faster imbibition, germination, and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to a lower protease activity, seed structure disintegration, and higher anti-nutrient levels in comparison to GZL, DL, and ZF. Moreover, the digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. The results suggested that the germination process improved protein digestibility and the health-promoting potential of lentil and fava bean proteins although these changes were more evident in DL due to its rapid imbibition, faster germination, and sprout development. This study will provide important information for either plant breeders to develop legume varieties with permeable seed coats or food producers that could use dehulled seeds for efficient production of sprouts as sustainable food sources of plant proteins with improved nutritional and healthy properties.

Mineral Composition, Phenolic Content, and In Vitro Antidiabetic and Antioxidant Properties of Aqueous and Organic Extracts of Haloxylon scoparium Aerial Parts.

Haloxylon scoparium is a plant widely used in traditional medicine for the treatment of diabetes. Hence, this study focuses on the mineralogical and chemical composition and evaluation of the antidiabetic and antioxidant activities of the aerial part of this species. The mineralogical analysis was done by inductively coupled plasma atomic emission spectrometry (ICP-AES). The phytochemical study consisted in the preparation of different extracts from the aerial part by aqueous and organic extraction using Soxhlet and cold maceration. Then, phytochemical screening was performed on the plant powder and on the extracts, which is completed by spectrophotometric quantification of total polyphenols, flavonoids, and catechic tannins. The evaluation of antidiabetic activity was done by three enzymes: a-amylase, a-glucosidase, and ß-galactosidase, and that of antioxidant activity was done by five methods: H2O2, DPPH, ABTS, FRAP, and reducing power (RP). Mineralogical analysis revealed the presence of iron, potassium, magnesium, phosphorus, sodium, copper, calcium, strontium, selenium, and zinc. The studied part is rich in alkaloids, flavonoids, catechic tannins, and saponins. The methanolic extract is rich in total polyphenols (161.65 ± 1.52 Ug EAG/mg E), and the ethyl acetate extract has high levels of catechic tannins (23.69 ± 0.6 Ug EC/mg E). In addition, the decoctate expresses a high flavonoid content of 306.59 ± 4.35 Ug EQ/mg E. The in vitro evaluation of the antidiabetic activity showed that the decoctate has a higher inhibitory capacity on a-glucosidase (IC50 = 181.7 ± 21.15 ug/mL) than acarbose (IC50 = 195 ± 6.12 ug/mL). The results of the antioxidant activity showed that the methanolic extract and the decoctate present a percentage of hydrogen peroxide (H2O2) scavenging (20.91 ± 0.27 and 16.21 ± 0.39%) higher than that of ascorbic acid (14.35 ± 0.002%). Positive correlations obtained between the total polyphenol content and the antioxidant activity of the extracts were studied. A positive correlation of a-amylase inhibitory activity was also recorded with the antioxidant activity tests.