Levels Of Bioethanol Research Articles

Bioethanol Levels from Corn Cob Waste: Effect of Fermentation Time and Saccharomyces cerevisiae Yeast Amount (Zea mays)

This study aims to determine the highest levels of bioethanol produced through the fermentation process, by looking at the effect of variations in the amount of Saccharomyces cerevisiae, immobilization, and fermentation time, as well as the effect of the amount of Saccharomyces cerevisiae and fermentation time on the ethanol content produced. The highest ethanol content was 39.5 percent in the bioethanol test, with the amount of Saccharomyces cerevisiae 8 grams and fermentation time of 9 days. The treatment given to the number of immobilized Saccharomyces cerevisiae cells and the length of time of fermentation had a major effect on the ethanol content produced, as shown by Anova.

FERMENTASI BIOETHANOL DARI BAHAN BAKU BIJI BUAH-BUAHAN MENGGUNAKAN RAGI ROTI DAN RAGI TAPE

The rapid increase in population resulted in the use of non-renewable fossil energy is increasing and its availability is dwindling. So its dependence must be immediately reduced by using renewable alternative energy sources such as biomass energy. This research aims to find out the potential of fruit seeds (durian, mango, jackfruit) as raw materials for making bioethanol, to find out the fruit seeds (durian, mango, jackfruit) that produce the highest levels of bioethanol, and to find out the effectiveness of adding seeding solution to the fermentation process. The research was conducted in the Laboratory of the Faculty of Engineering, Malahayati University, on a laboratory scale (300 ml). The methods used include the pretreatment stage, namely the collection of materials, the hydrolyse stage, namely the addition of a solution of 2.5% H2SO4, the seeding stage of seeding bacteria in yeast with the addition of a solution of brown sugar, the fermentation stage, and the distillation process. The results showed mango seeds provided the best bioethanol levels (4%) compared to bioethanol levels from durian seeds and jackfruit seeds. Yeast tape gives better results than bread yeast.

Effect of hydrogen peroxide acetic acid pretreatment on kapok (ceiba pentandra) fruit peel waste for bioethanol production using separated hydrolysis and fermentation methods

The increase in the world's population has an impact on the increasing demand for fuel energy. With the increasing need for fuel, it causes the depletion of fossil fuel sources. For this reason, the search for alternative energy sources for fuel must be developed so that it can be applied for mass use, one of which is bioethanol. Bioethanol is a renewable energy source that has the potential to be developed in Indonesia. Bioethanol is ethanol from the fermentation of sugar, starchpatian or lignocellulosic biomass. This study examines the effect of hydrogen peroxide acetic acid (HPAC) pretreatment of kapok fruit peel waste on the cellulose, hemicellulose, and lignin content produced, examines the effect of fermentation pH on the levels of bioethanol produced, and examines the effect of fermentation time on the levels of bioethanol produced. In the research of bioethanol production, the variables used were pretreatment of raw materials, pH of fermentation (4, 4.5, 5), fermentation time (3, 5, 7 days). The results showed that the pretreatment of HPAC was able to remove 90% of the lignin content in kapok fruit peel waste, while the optimum pH and optimum time for fermentation were obtained at pH 4.5 and day 5 with the content of bioethanol 8.268% and the highest yield of bioethanol is 8.78%.

EFFECTS OF YEAST WEIGHT AND STARTER VOLUME ON THE PERCENTAGE KEPOK BANANA BASED BIOETHANOL

The low availability of energy resources is in line with the increasing human need for these resources, such as fossil fuels which are increasingly expensive because they are difficult to obtain right. Bioethanol is an environmentally friendly liquid produced from the glucose fermentation process from carbohydrate sources of natural raw materials with the help of microorganisms in baker's yeast. The focus of the research is on the waste of kepok banana peels because it has a high starch content of 18.5%. In addition, kepok banana peel waste is easy to obtain and has not been widely used by the community. This study aims to determine the effect of yeast weight and starter volume in the manufacture of bioethanol and the quality of the bioethanol produced by comparing the levels with the reference levels of bioethanol as an alternative fuel. The bioethanol obtained from the starch of the kepok banana peel has gone through a fermentation process with Saccharomyces cerevisiae and refined into pure bioethanol. Based on the related literature review, it shows that the greater the weight of yeast and the volume of starter used in the manufacture of kepok banana peel bioethanol influences the yield of bioethanol content which also increases so that it affects the quality of the bioethanol produced. However, the level of bioethanol that is obtained has not reached the standard range of SNI 7390:2012 with a minimum level of bioethanol as a substitute for fuel being 94.0-95.5%. Thus, bioethanol cannot be used as a substitute for fossil fuels and further research is needed with the use of the optimum amount of yeast and starter volume and re-purification.

Sugar and ethanol production potential of sweet potato (Ipomoea batatas) as an alternative energy feedstock: processing and physicochemical characterizations

This study focuses on the processing, characterization, and sugar and ethanol production potential of red-fleshed sweet potatoes (RFSP) and white-fleshed sweet potatoes (WFSP). These feedstocks were used for the production of sugar; and bioethanol from its pulp by the action of five different microbes. The characterization of raw sweet potatoes and desired products of raw sugar, and bioethanol were carried out through proximate analyses, Fourier transforms infrared spectroscopic (FTIR) method, measurement of pol% by using a Refractometer, Polarimeter, Saccharimeter. The proximate analyses of feedstocks show the presence of a respectable amount of dry solids 25 ± 0.03g/100g with a lower amount of fat (0.025 ± 0.002) and ash (0.533 ± 0.076) contents make them promising crops for the production of sugar and ethanol. Comparatively, RFSP raw sugar (oZ: 95.25 ± 0.05) is considered purer than WFSP raw sugar (oZ: 94.6 ± 0.015). FTIR spectrums of the presently studied raw sugar and bioethanol have characteristic bands. It shows that the raw sugars products are rich in sucrose content, and confirms that the bioethanol was produced from the selected raw materials is at a satisfactory level. The efficiency of microbes was evaluated by taking a sample from the fermented wash to measure the residual sugar in (oBrix). Comparatively, fermented wash with sweet potato extracted yeast was found 14% Brixo (consume 86% of pulp) in RFSP, and 17% of Brixo (consume 83% pulp) in WFSP within 24 hours of fermentation. The alcohol level of bioethanol's produced from RFSP and WFSP pulps was tested using Ebuliometer and the result was found to be ranged 78 °C – 80 °C which is closer to the boiling point of absolute anhydrous alcohol (78.3 °C). Thus, the results of the present study proved that the sweet potato and its pulp are considered as a potential alternative sugar/energy feedstock.

PENGARUH JENIS NUTRIENT DAN WAKTU TERHADAP EFISIENSI SUBSTRAT DAN KINETIKA REAKSI FERMENTASI DALAM PRODUKSI BIOETANOL BERBAHAN BAKU BIJI DURIAN

Bioethanol is one source of energy that can be obtained from vegetable fermentation. This bioethanol production utilizes durian seed waste as a fermentation media. The purpose of this study was to determine the concentration of urea and NPK and the best time in making bioethanol from durian seeds by Saccharomyces cerevisiae, to study the kinetics of the fermentation reaction, determine the efficiency of the substrate during the fermentation process, and to determine the levels of bioethanol that can be produced from durian seed flour. This research was conducted by adding nutrients in the form of urea and NPK, respectively 3% and 4% with fermentation time for 48, 72, 96, 120, 144, and 168 hours. Based on the research results, the levels of starch contained in durian seed flour were 45.38%. Conversion of starch to sugar by 37,8167 grams. The best substrate efficiency is obtained by urea 3% with 120 hours fermentation time of 99,24% with residual sugar content of 0,76%. The highest levels of bioethanol in the urea sample were obtained at the addition of 3% urea, ethanol content of 57,1429% with a fermentation time of 120 hours and a Vmax of 1,2397 g/mL.hour. The best substrate efficiency obtained in NPK samples was found in NPK 4% fermentation time 144 hours by 99,16% with residual sugar content of 0,84%. The highest levels of bioethanol in NPK samples were obtained by adding 4% NPK, ethanol content of 48,5714% with 144 hours fermentation time and Vmax of 1,0116 g/mL.hour.

Application of Pineapple Skin Waste as a Source of Biosolvent for Use as Wax Inhibitor

Wax paraffin deposition is a problem faced in the pipeline for petroleum industries that they blockage the partial or full inside the pipe, which will decrease the production rate. One of the treatments is to use the preventive methods called wax inhibitors which are expected to inhibit the crystallization of paraffin wax, and bio-solvent is included. Hydrolysis and fermentation technique are used to produced bio-solvent. Hydrolysis aims to break lignin and hemicellulose, damage the crystal structure, and increase the porosity of the material. At the same time, the occurrence of pentose changes and some glucose into ethanol is present in the fermentation process. Then, purified by the distillation process to obtain bio solvent products that are applied with waxy crude oil can reduce the pour point value of crude oil. From the hydrolysis process with five variations of acid percentage, the amount of reducing sugars increase. By increasing temperature from 50 ° C to 100 ° C, the reducing sugars continues to increase until it reaches the optimal point of 18.2 ° Brix. The amount of inoculant also affects the level of bio-solvent where the optimum results using inoculants are 0.015 g/mL, which produces 6% levels of bioethanol. The high ethanol content of 2% had a density value of 0.979 g/mL. The best °API at 5% is 13.901, and the average value is about 13.0945, where the best viscosity values for ethanol content of 6% are 0.814. Bioethanol testing using waxy crude oil is carried out with the bioethanol content of 6%. The addition of the ethanol contents only decreased the pour point 2-3℃. At sample, #LGK19 experienced a 3°C drop in pour point from 45°C to 42°C. Therefore, it can be concluded that bioethanol used as a solvent can potentially inhibit paraffin deposition.

An evaluation based on energy and exergy analyses in SI engine fueled with waste bread bioethanol-gasoline blends

In this study, the operation of a single cylinder, four stroke, SI engine powered by fuels created by blending two different bioethanol with gasoline was evaluated on the basis of energy and exergy analyses. Within the scope of the study, test engine was tested by operating it with fuel blends, prepared by adding both bioethanol, one of them was produced from waste bread and the other, originating from sugar beet, was supplied, to gasoline at different rates by of 5, 10, 15, 85 and 100% under five different engine loads at maximum torque speed, 2500 rpm. The variation of energy and exergy parameters was examined by comparing the blends, prepared to have similar volumetric content, each other and by comparing all with gasoline. As a result of the study, it was determined that the addition of bioethanol had generally decreasing effect on energy loss rate, exergy loss rate and exergy destruction rate. For fuel blends containing low levels of bioethanol, variation of ethanol has created little differences on all energy and exergy parameters but bioethanol type clearly showed its effect when bioethanol rate increased to 85% and 100%. Although waste bread bioethanol could not catch sugar beet bioethanol in terms of thermal efficiency (14.4% for E100b and 16.4% for E100 as load average) and exergetic efficiency (12.9% for E100b and 14.7% for E100 as load average), both bioethanol have been found to improve both thermal efficiency (12.2% for E0) and exergetic efficiency (11.4% for E0) than that of gasoline.

Proses Optimasi Produksi Bioetanol dari Limbah Serat Buah Sawit dengan Metode SHF

The Indonesian government is trying to equalize development including the energy sector. The government launched the use of alternative energy starting in 2008 with a blueprint for searching and utilizing new renewable energy sources in Indonesia through biofuels, one of the alternative energy developed is bioethanol. The research objective is the optimization of the SHF method bioethanol production process from palm fruit fiber waste.
 The experimental design uses central composite design with variable H2SO4 concentration and fermentation time. The first stage in the study was by saccharifying the palm oil fiber waste by the hydrolysis method using H2SO4 (concentrations of 1 M, 2 M, and 3 M). Then the next stage is fermentation process (fermentation time is 1 day, 2 days, 3 days, 4 days and 5 days). Sugar content analysis was carried out in the fermen solution and analysis of bioethanol levels in each running experiment. Then the optimization is done with the response surface method (RSM).
 Based on the research, the optimum condition of the bioethanol production process is H2SO4 concentration of 2.76 M with a fermentation time of 4.64 days which will produce bioethanol levels of 28.6027 g/L.

PENINGKATAN KADAR BIOETANOL DARI KULIT NANAS DENGAN BEKAS CRACKING PERTAMINA RU III TERAKTIVASI SECARA KIMIA

Bioetanol adalah etanol yang dihasilkan dari fermentasi glukosa (gula), diikuti oleh proses distilasi. Metode lain selain penyulingan diperlukan untuk meningkatkan bioetanol yang dihasilkan. Penelitian ini bertujuan untuk meningkatkan kadar bioetanol dari kulit nanas menggunakan katalis perengkahan dengan membandingkan efektivitas penggunaan adsorben yang diaktifkan secara kimia menggunakan basa (NaOH) dan asam (HCl). Adsorben yang digunakan dalam penelitian ini adalah katalis perengkahan RU III, katalis FCC. Adsorben adalah sebagai pengganti adsorben zeolit alami, yang relatif mahal dibandingkan dengan katalis FCC. Hasil penelitian menunjukkan bahwa rasio yang paling efektif untuk kadar bioetanol adalah 10 mL: 6 gr adsorben yang diaktifkan oleh HCl, adalah 63,73%.

UTILIZATION OF RESIDUAL CARRAGEENAN EXTRACT FROM Eucheuma cottonii SEAWEED INTO BIOETHANOL

In the process of processing seaweed will produce residual waste from carrageenan extraction, and the residue still contain cellulose, lignin, hemicellulose, pectin, and other organic materials that can be processed into bioethanol. This research aimed to utilize the residual carrageenan extracted from seaweed Eucheuma cottonii into bioethanol. The research method includes acid hydrolysis process using 3% sulfuric acid at a temperature of 70-80oC for 30 minutes, followed by a fermentation process using yeast Saccharomyces cerevisiae with a ratio of 1: 0.006 for hydrolyzate and yeast, fermentation time treatment 1, 3, 6, 9 and 12 days at temperature 25o-30oC. Fermentate at 78oC, measured in degrees of acidity (pH), volume, and levels of bioethanol. The results showed that the residual carrageenan extract containing carbohydrates as un-extracted carrageenan was 5.01%, hemicellulose was 7.12%, cellulose was 0.96%, and lignin was 8.26%. The level of bioethanol produced from the residual carrageenan extraction was 2.57% and, the yield was 32.64% with a fermentation time of 6 days as the optimal time.

A Novel Immobilization Method of <i>Saccharomyces cerevisiae</i> on Fermentation of Nipa Palm Sap for Fuel Grade Bioethanol Production

Nipa palm (Nypa fruticans) spreads abundantly in the mangrove forests of eastern coast of Sumatera Island, Indonesia. Nipa palm sap can be used as a very high-gravity (VHG) substrate for fermentation. In this research, batch fermentation of nipa sap with initial sugar content of 262.713 mg/ml using immobilized Saccharomyces cerevisiae yeast cells was studied. Immobilization of the yeasts in Na-alginate by droplet method and addition of 0.2% v/v Tween 80 and 0.5g/l ergosterol to the immobilized cells were first carried out. Then, the effect of cells weight percentage (5, 10, 15, and 20% w/v) and fermentation time (24, 36, 48, 60, 72, 84, and 96 hrs) on the bioethanol production were investigated. After, the analysis of bioethanol concentration was investigated using Gas Chromatography. The bioethanol production increased with the fermentation time until reaching a maximum value at all cell weights. Except with the 20% w/v, this peak was followed by a decrease in the bioethanol production at cell weights of 5, 10, and 15% w/v. This phenomenon may be explained by degradation of bioethanol into acetic acid resulting in the decreased concentration at the end of fermentation. The formation of acetic acid was characterized by decreases in the pH values of the fermentation medium. On the contrary, the bioethanol level tended to increase until the end of fermentation with the immobilized yeast cells of 20% w/v. High number of available immobilized yeast cells at the end of fermentation, accumulation of bioethanol produced at earlier times, and no further conversion of bioethanol to acetic acid could be the reasons for this increase. The optimum conditions for bioethanol production were 20% w/v cell weight and 96 hr fermentation time, at bioethanol concentration of 17.57% v/v.

EFFECT OF YEAST CONCENTRATION AND TYPE ON BIOETHANOL PRODUCTION FROM SUGARCANE BAGASSE

Sugarcane bagasse is one of the potential biomass as raw material for bioethanol production. This study aims to determine the effect of concentration and type of yeast on the characteristics of bioethanol produced from bagasse bagel delignified with a mixture of NH4OH-NaOH in a ratio of 1:20. This study uses a Completely Randomized Design (CRD) with 2 factors: yeast concentration (0.4%, 0.6% and 0.8%) and the type of yeast (tape yeast and bread yeast). The results of this study indicate that Bioethanol levels increase with increasing yeast concentration. The highest levels of bioethanol were obtainedat the use of 0.8% bread yeast, ie 14.16%

Bioethanol Levels of Dragon Fruit (Hylocereus polyrhizus) Peel with the Addition of Blend Crude Cellulase Enzyme from <i>Trichoderma reesei</i> and <i>Aspergillus niger</i>

The petroleum fuel crisis shows that Indonesia's fossil energy reserves are limited. It is necessary to develop an environmentally, friendly and sustainable alternative energy, one of which is bioethanol. This study aims to determine the bioethanol levels of dragon fruit (Hylocereus polyrhizus) peel with the treatment of cellulase enzymes from Trichoderma reesei and Aspergillus niger. This research was an experimental study that uses steps such as making dragon fruit peel substrate and filtrate, cellulose degradation with enzymes from Trichoderma reesei and Aspergillus niger and inoculating with yeast (Saccharomyces cerevisiae) with a fermentation time of 96 hours and then measured reducing sugar levels with the method of DNS, distillation, and the measurement of bioethanol levels using alcohol meters. The results have shown that using enzymes from Trichoderma reesei and Aspergillus niger can increase the reduction of 49.68 % sugar levels in the treatment of T.reesei: A.niger (3: 1) and produce the highest bioethanol level, which is 2.46 % in the treatment of T.reesei: A.niger (2: 1)

Synthesis of Bioethanol from Cocoa Pod Husk Using Zymomonas Mobilis

Nowadays, energy issue is one of the interesting discussions among the researchers and energy stakeholders. This is due to the reducing of the main source of world energy which consists mostly of non-renewable energy derived from fossils of living things millions of years ago. Alternatively, there are some alternative energy that promising in the future such as bioethanol from plant or fruits. For that reasons, the aim of this research is to make bioethanol from the peel of cocoa fruit. Cocoa pod husk can be used as source of bioethanol through a fermentation process with the help of bacteria. This study will examine the ethanol content in fermented cocoa pod husks. The fermentation time was 0 days, 2 days, 4 days, 6 days, 8 days. The Zymomonas mobilis bacteria is used in the fermentation process and their percentage is 8%v/v, 10%v/v, 12%v/v, 14%v/v, 16%v/v.In the process of fermentation of cocoa pod husk with Zymomonas mobilis inoculum on the 3rd day to 9thshowed an increase. On the eleventh day in all conditions the addition of a starter was decreased. The best condition when fermentation takes place on the 8th day with a starter dose of 14% and obtained an alcohol content of 10.62%.The results of this study indicate that the addition of Zymomonas mobilis starter and fermentation time affect the levels of bioethanol produced. The addition of a starter as much as 14% and the fermentation time for 8 days are the best condition in this study.

The Effect Of Chloride Acid (Hcl) Concentration And Fermentation On Bioethanol Levels From Breadfruit (Artocarpus Artilis) Substrate

At present, the public demand for fuel oil (BBM) is increasing inversely proportional to its availability. One of the efforts to reduce people’s dependence on BBM is to utilize alternative vegetable-based energy such as bioethanol. Bioethanol is a fermented product that can be made from a substrate containing carbohydrates. Breadfruit (Artocarpus artilis) is one of the agricultural products that have a fairly high starch content of 89% and is not included as a staple food source in Indonesia. Utilization of breadfruit is still not optimal because breadfruit is only used as a snack or made into flour. This study aimed to make bioethanol with breadfruit raw material (Artocarpus artilis). The method of this study consisted of two stages, the first step was the hydrolysis of breadfruit flour with hydrochloric acid (HCL) catalyst. The concentrations of HCL used in this study were 1%, 1.5%, and 2%. The second stage of fermentation using Saccharomyces cerevisiae was with 72 hours fermentation time, 120 hours and 168 hours. From the results of the research, it was found that the highest levels of bioethanol to the treatment of HCL 2% concentration and fermentation duration of 168 hours was 17.6%.

ANALISIS HASIL FERMENTASI PEMBUATAN BIOETANOL DENGAN VARIASI WAKTU MENGGUNAKAN BAHAN (SINGKONG, BERAS KETAN HITAM DAN BERAS KETAN PUTIH)

This study aims to determine the levels of bioethanol from (cassava, black sticky rice, and white sticky rice) with the addition of 10 grams of yeast mass, with a variation of fermentation time of 48 hours, 72 hours and 96 hours and knowing the ethanol levels in accordance with National Standards Indonesia (SNI). This research was carried out by hydrolysis or boiling of ingredients (cassava, black sticky rice, and white sticky rice) using 800 ml of distilled water with 30 minutes, then fermentation using yeast or (saccharomyces cereviseae) and distillation using a tool. Complete destilator to obtain ethanol from fermentation results which is then tested with a pen refractometer to determine whether or not ethanol is formed from the distillation process. The samples were then tested for ethanol content using the Gas Chromatography tool. The results of this study are known by conducting a test using the Gas Chromatography tool to determine the ethanol content contained in the distilled sample. The results of cassava ethanol content with 72 hours fermentation time with ethanol were 98.41%, black sticky rice with 96 hours fermentation time the ethanol content was 94.96%, and white sticky rice with 96 hours fermentation time the ethanol content was 96.67%.

Alcohol dehydration of palm sugar (Arrenga pinnata) from Lalolae Village, East Kolaka, Southeast Sulawesi

This research aimed to obtain ethanol from fermented palm sugar with high purity. There are two main techniques used in this study to increase the purity of alcohol, namely modified multilevel distillation and the use of molecular sieve as the principal dehydrator agent. The process carried out to produce ethanol uses a distillation and dehydration process, with raw materials fermented using Saccharomyces cerevisiae and traditional technology. At first, prepare two column reflux distillation apparatus had been designed and constructed successfully to prepare bio ethanol. The sugar content of palm sugar used was 12.4%. Bioethanol is produced from 10% inoculum concentration and variations in fermentation time of 12 hours, 24 hours 36 hours, and 48 hours at pH 5. The results of measurement using alcoholmeter, the highest alcohol concentration was obtained at the condition of 36 hours fermentation time by 9%. The distillation and dehydration process is carried out continuously at a distillation temperature of 79 °C with a mass of 20 gram molecular sieves. The results of measurement of alcoholmeter obtained bioethanol levels of 54%.

Pengaruh Penambahan Molase pada produksi Bioethanol dari Limbah Padat Industri Pati Aren

Penelitian ini telah dilakukan untuk mengetahui pengaruh penambahan molase terhadap produksi bioetanol dari limbah padat industri pati aren. Tahapan penelitian ini meliputi persiapan bahan baku, delignifikasi limbah padat pati aren, analisis kadar lignin, produksi enzim, fermentasi anaerob dan analisa kadar etanol. Delignifikasi limbah padat pati aren menggunakan larutan NaOH 3%. Produksi enzim dihasilkan dari jamur A. Niger dan Trichoderma sp. Fermentasi menggunakan 5 gram sampel hasil delignifikasi ditambahkan larutan nutrisi, gabungan enzim selulase dari A. niger dan Trichoderma sp. dengan perbandingan (1:2), larutan molase 35 % dan Saccharomyces cereviseae dari ragi roti. Analisis kadar etanol dilakukan setiap hari hingga hari ke-6. Delignifikasi menggunakan larutan NaOH 3% dapat menurunkan kadar lignin pada sampel 17,15%. Penambahan molase mengandung gula 35% sebagai sumber gula pada limbah padat pati aren akan meningkatkan kadar bioethanol dari 0,0904 % menjadi 3, 5407 %, sedangkan. molase dengan kadar gula 40 % menghasilkan kadar bioethanol lebih rendah. Semakin tinggi kadar gula akan diperoleh kadar bioethanol semakin tinggi, tetapi apabila kadar gula terlalu pekat mengakibatkan proses fermentasi lambat pada produksi bioethanol.

ANALISIS HASIL FERMENTASI PEMBUATAN BIOETANOL DENGAN VARIASI MASSA RAGI MENGGUNAKAN BAHAN (BERAS KETAN HITAM, BERAS KETAN PUTIH DAN SINGKONG)

This study aims to determine the levels of bioethanol from (black sticky rice, white sticky rice and cassava rice) with variations in yeast mass as much as 5 grams, 10 grams and 15 grams knowing the optimum time variation and knowing ethanol levels according to SNI. This research was carried out by hydrolysis or boiling of ingredients (black sticky rice, white sticky rice and cassava rice) using distilled water for 30 minutes, then fermentation using yeast saccharomyces cereviseae and distillation process using a complete destilator to obtain ethanol from fermented then tested with a pen refractometer to determine whether or not ethanol is formed from the distillation process. The selected sample was then tested for ethanol content using a gas chromatography tool. The results of this study are known by testing gas chromatography to find out the ethanol content contained in the distilled sample, the results of ethanol, black glutinous rice with 96 hours fermentation time 92.29% ethanol, and white glutinous rice with fermentation time 96 hours of 100% ethanol and cassava with 96 hours of fermentation time of ethanol content of 98.10%. Keywords: Black Sticky Rice, White Sticky Rice, Cassava, Fermentation, Ethanol