Leukocyte Index Research Articles

Вплив комплексного лікування пневмоконіозу у поєднанні з хронічним обструктивним захворюванням легень на деякі показники інтоксикації у працівників гірничорудної та металургійної промисловості

Objective:We studied the efficiency of the comprehensive treatment of ore mining and iron and steel industry workers having the pneumoconiosis combined with chronic obstructive pulmonary disease in terms of intoxication indicators as an assessment of measures aimed at treatment and prevention of this occupational pulmonary pathology. Results: It was established that conducted treatment in the patients, ore mining and iron and steel industry workers with the use of bronchodilator, mucoregulator, anti-inflammatory agent of a complex vitamin medication, as well as sessions of hyperbaric oxygenation at 1.3 atm led to a decrease in the activity of inflammation processes with regard to the content in the peripheral blood of the total number of leukocytes, their stab and segmented subpopulations, and a decrease in most intoxication indices: the leukocytic index of intoxication, the granulocytic-agranulocytic index, the general index of intoxication, the sinistroposition index, the index of the ratio of leukocytes to erythrocyte sedimentation rate, which indicates a decrease in the systemic inflammation activity with the simultaneous suppression of intoxication phenomena. In comparison with the patients with other occupational lung pathology, the comprehensive treatment led to a more significant decrease in the content of segmented and stab leukocytes, as well as the main integral intoxication indices: the leukocytic index of intoxication, the granulocytic-agranulocytic index, and the general index. Thus, the comprehensive treatment of ore mining and iron and steel industry workers having the pneumoconiosis combined with the chronic obstructive pulmonary disease is an important link in the system of measures aimed at treatment and prevention ofthis occupational pulmonary pathology. Conclusions: In the ore mining and iron and steel industry workers having the pneumoconiosis combined with the chronic obstructive pulmonary disease,the comprehensive treatment with the use of bronchodilator, mucoregulator, nebulizer-administered anti-inflammatory agent, a complex vitamin B medication administered intramuscularly, as well as sessions of hyperbaric oxygenation led to an improvement in some inflammation indicators and intoxication indices which is a predictor of a positive course of the disease. The assessment of the results of the conducted comprehensive treatment of the workers indicates a significant positive dynamics of intoxication processes and makes it possible to regard this treatment as an important link in the system of measures aimed at treatment and preventionofthisoccupational pulmonary disease.

THE INFLUENCE OF SYNTHETIC INDOLICIDIN ANALOGUES ON THE HE MATOLOGICAL INDICES IN THE RATS WITH EXPERIMENTAL PERIODONTITIS

Synthetic analogues of indolicidin are considered as potential antibiotics, since they have a wide spectrum of antimicrobial activity. We used synthetic analogues of indolicidin No. 7 and 8, which were used to correct experimental periodontitis in Wistar rats. Peptides were injected intraperitoneally at a dose of 500 pg/kg for 7 days after periodontitis modeling. The anti-inflammatory effect of indolicidin analogues was assessed by changes in integral hematological indices. A statistically significant effect of the studied indolicidin analogues on the neutrophil-to-monocyte ratio and the leukocyte index was established, which indicates to the effect of peptides on the macrophage-monocytic cell pool, as well as the affector and effector mechanisms of the humoral immune response.

Features of the clinical course of measles infection during the measles outbreak in 2018-2019 in Ukraine

Measles is an infectious disease characterized by an acute onset, a short prodromal period, presence of febrile, catarrhal and intoxication syndromes, as well as the development of typical enanthema and exanthema. Despite the active work of WHO to eliminate measles and the introduction of a vaccination program in developing countries, measles remains an urgent problem and the cause of high mortality rates among both children and adults. One of the dangerous complications of measles infection is the respiratory tract involvement in yhe form of out-of-hospital pneumonia, which occurs in 10-34% of patients. The out-of-hospital pneumonia was one of the main complications among the patients with measles who were treated in the Kharkiv regional clinical hospital and was registered both in patients with severe and moderate disease. That is why it was interesting to study the clinical and laboratory features of the course of measles infection in the adult population at the present stage. Objective: to determine features of the clinical course of measles infection and the factors that are prerequisites for the development of complications. Materials and methods. The study included 65 patients who were treated in the Kharkiv regional clinical infectious hospital in 2018-2019. Based on the clinical course of the disease, the severity and the presence of complications, the patients included in the study were divided into 4 groups. The control group consisted of 20 healthy voluntary donors who did not have exclusion criteria. On the day of admission to the hospital and on the 10th day of stay in the hospital, based on the data of a clinical blood test, we calculated the cellular reactivity leukocyte indices (CRLI) – the white blood cell shift index (WBCSI), the white blood cell index (WBCI) and the D. A. Ivanov immunoreactivity index (IRI). Results and discussion. The study shows that the patients from groups 1 and 2 on the 10th day of hospitalization showed a complete clinical recovery and was discharged from the hospital, whereas patients from groups 3 and 4 on the 10th day showed a clinical improvement and were transferred for further treatment to the general department. When assessing the CRLI on the day of admission to the hospital, an increase in the WBCSI in patients from groups 2, 3 and 4 was found, with the greatest increase observed in patients from group 4. In patients from group 1, on the contrary, a decrease in the WBCSI and and an increase in WBCI were determined, which indicates the activation of the lymphocytic link of the immune response. Conclusions. The development of measles infection in the adult population is characterized by changes in the leukocyte indices of cellular reactivity, which are markers of the functional activity of the immune system. The moderate course of the disease is accompanied by a tendency to normalization of the indices that characterize the activity of the inflammatory process, while its severe course is characterized by an increase in the values of these indices and a decrease in the activity of the lymphocytic link of the immune response. Assessment of changes in the parameters of leukocyte indices in dynamics makes it possible to predict the clinical course of the disease and the risk of complications, to assess the adequacy of the therapy, as well as to identify the need for immune correction therapy.

Economic assessment of anti-stress therapy in the production of meat in poultry farming

The paper presents the results of the effectiveness of anti-stress therapy and its economic assessment when growing broiler chickens in a poultry farming against the background of the use of a stress-protector antioxidant-complex (SPAO-complex) in broiler chickens. The pharmacological complex was used in the form of a drink at a dose of 185 mg/kg of live weight for 5 days during periods of the greatest influence of stress factors, namely at the stage of vaccination associated with the greatest immunological load (at 10-14 days of age) and (or) at the stage of final fattening. The effectiveness of antistress therapy was assessed by the morphological parameters of poultry blood, leukocyte indices, the yield of poultry meat and the quality of carcasses. The economic efficiency was assessed by the ratio of the economic effect and veterinary costs. A relative decrease in the total level of leukocytes and a change in leukocytes of different types was found: a decrease in agranulocytes and an increase in agranulocytes, a decrease in the value of the Krebs index, the leukocyte index of intoxication, an increase in the lymphocyte index, the ratio of agranulocytes and granulocytes and lymphocyte-granulocyte index. The use of the SPAO-complex in broiler chickens in the form of a drink at a dose of 185 mg/kg of live weight for 5 days due to the growth rate and an increase in live weight helped to reduce the impact of stress factors and led to an increase in meat yield by 2.34-4.92% and a decrease in injuries before slaughter, which was reflected in a decrease in the level of carcass defect from 20-40%. It influenced the value of the economic effect which amounted to 70.08-145.2 rubles. The value of veterinary costs for different anti-stress therapy regimens ranged from 9.35 to 101.32 rubles. The economic efficiency of using the SPAO-complex at a dose of 185 mg/kg of live weight per day for 5 days per one ruble of costs during the period of intensive vaccination was 16.7 rubles, during the period of final fattening it was 0.81 rubles and during periods of intensive vaccination and final fattening it was 1.52 rubles.

Liver function recovery of COVID-19 patients after discharge, a follow-up study.

Objective: The aim of this study was to observe the liver function recovery of COVID-19 patients after discharge.Patients and Methods: A total of 253 discharged COVID-19 patients in Shenzhen city, China were selected. The clinical characteristics of these patients were assessed. A 2-month follow-up and laboratory hematology test were performed to examine the status of patients' liver function.Results: Patients combined with liver diseases, especially fatty liver, are more likely to progress to severe condition (P<0.05). Patients in severe condition and those with liver diseases have higher rates of liver injuries during hospitalization, characterized by a significant increase in alanine aminotransferase (ALT) and aspartate aminotransferase (AST, P<0.01). The ALT, AST/ALT, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), and A/G levels showed significant differences in comparison with the control group (P<0.05, and P<0.001); and the outlier ratio of A/G, ALT, GGT and ALP of patients remained abnormal higher within 14 days after discharge (P<0.001). Liver injuries of COVID-19 patients may be related to the epidemiological characteristics, clinical indexes, basic diseases, symptoms, drug treatment during hospitalization and the complications. Indicators of liver function were correlated with cardiac function, renal function, thyroid function, lipid metabolism, glucose metabolism, immune index, leukocyte, erythrocyte, hemoglobin and platelet related indexes. The outlier ratio of TP, ALB and GLB remained extremely low throughout the follow-up period; the outlier ratio of ALT, AST and GGT decreased below 10% from a high level at 40 days after discharged. However, the outlier ratio of A/G, AST/ALT and ALP remained high during the follow-up period.Conclusions: Abnormal liver function might indicate worse recovery of COVID-19 patients. Changes in liver function should be emphasized during long-term follow-up of COVID-19 patients after hospital discharge; the necessity of employing appropriate interventions for liver function repair should be emphasized.

Hematological Profile in COVID-19, Whether It Matters in Children

Coronavirus disease 2019 (COVID-19) is an acute respiratory and systemic illness, and is a public health emergency which has involved all the continents. Though limited data is available for infection in pediatric population, infection seen in children is of milder form. During this pandemic, documentation of laboratory predictors to discriminate between mild and severe forms of this infection would be helpful. Various hematological parameter alterations in the pediatric COVID-19 cases show anemia, thrombocytopenia, eosinopenia, and inconsistent leukocyte indices. It may be too early to say right now as to what extent will these alterations help us in outlining the severity of disease or transmission of disease. However, it will be pertinent to understand these abnormalities, and keep track of them, so as to understand the disease, as the pandemic unfolds.

ГЕМАТОЛОГИЧЕСКИЕ ИНТЕГРАЛЬНЫЕ ПОКАЗАТЕЛИ В ОЦЕНКЕ КЛЕТОЧНОЙ РЕАКТИВНОСТИ ОРГАНИЗМА ПРИ КОРОНАВИРУСНОЙ ИНФЕКЦИИ COVID-19

The article analyzes the disorders in homeostasis diagnosed during the development of acute viral infection SARS-CoV-2 in patients. Hematological changes in patients with coronavirus infection were characterized by the development of disintegration of humoral mechanisms of regulation with the initiation of a systemic inflammatory reaction with an increase in the leukocyte index of intoxication, activation of necrobiotic processes, an increase in the activity of macrophage-microphage nonspecific protection with a predominance of effector links of the immunological process. The development of immunocompromise in patients with a new strain of coronavirus infection is one of the determining factors in the course of the disease.

THE STUDY OF ACTION THE OZONE IN THE SURGICAL TREATMENT OF INFLAMMATORY PROCESSES BY DIABETES MELLITUS

The aim: was to improve the course of purulent wounds in diabetes by using physical therapies. Materials and methods: we investigated 122 patients with diabetes mellitus and wound processes. We divided all patients on the two groups. Our study groups were 50 patients who had therapy by ozone together with other general treatment and surgery treatment (daily dressings, ointments and solutions). Our control groups were 72 patients, who had treated only by general methods and surgery treatment (daily dressings, ointments and solutions).The patients of our explore group had received intravenous injection of saline ozone solution together with other general treatment and surgery treatment (daily dressings, ointments and solutions). Results: it had studied of indicators of oxidative modification of proteins, lipid peroxidation in blood plasma, antioxidant protection during this period, there were no significant changes in these parameters between the control and experimental groups. As the result of the study was found that the leukocyte index of intoxication in patients in the main and control groups was almost indistinguishable. Regarding the indexes of hematological index of intoxication, which decreased during the treatment of patients of the main group, and different from the patients of the control group, in which this indicator increased sharply. The result of the study of changes in the indicators of sorption capacity of erythrocytes showed that the level of sorption capacity of erythrocytes in patients in the main group during treatment, also decreased, compared with patients in the control group. Conclusions: Therefore, the use of ozone in the treatment of patients with complicated forms of diabetes does not cause a negative impact on the dynamics of homeostasis and indicators of the level of intoxication of the body. Ozone therapy has a beneficial effect on the course and wound process in patients with diabetes with purulent processes.

Impact of malaria co-infection on leukocyte indices of tuberculosis-infected participants at pretreatment, intensive, and continuation phase anti-tuberculosis therapy

BACKGROUND: Hematological changes involving all blood cells are some of the most common complications in both tuberculosis (TB) and malaria infection. The changes induced by malaria infection are diverse, and the first line anti-TB treatment regimen which involves two phases may alter these changes in TB participants co-infected with malaria (TB/MP).OBJECTIVE: In this study, we aimed to ascertain the impact of malaria co-infection on leukocyte indices of TB-infected participants at pre-treatment, intensive and continuation phase therapy.MATERIALS AND METHODS: In this cross-sectional study, 180 participants were recruited comprising; 60 (35 TB and 25 TB malaria) participants before treatment, sixty (36 TB and 24 TB-Malaria) participants after intensive phase treatment and sixty (27 TB and 33 TB-Malaria) participants after continuation phase therapy. Whole blood was used for the measurement of total (total white blood cell [TWBC]) and differential white cell count, Platelet count, and packed cell volume (PCV).RESULTS: Before initiation of treatment, TWBC, neutrophil, lymphocyte, platelet count, and neutrophil-lymphocyte ratio were significantly reduced (P = 0.041, 0.022, 0.046, and 0.026, respectively), whereas eosinophil count was significantly increased in TB/Malaria participants compared to TB participants (P = 0.043). There was no significant change in these parameters after intensive phase treatment (P > 0.05). However, after continuation phase treatment, PCV was significantly reduced, while eosinophil was significantly increased in TB/Malaria participants compared with TB participants (P = 0.046 and 0.045, respectively).CONCLUSION: Malaria co-infection induces the significant reduction in leukocyte indices of TB patients at pretreatment but not at the intensive and continuation phase anti-TB therapy except eosinophils count which was increased before treatment and continuation phase treatment.

ДИНАМИКА ЛЕЙКОЦИТАРНЫХ ИНДЕКСОВ В КРОВИ ЦЫПЛЯТ КРОССА «ХАЙСЕКС БРАУН» ПОСЛЕ ПРИМЕНЕНИЯ РАЗНЫХ ДОЗ ЭНРОФЛОКСАЦИНА В УСЛОВИЯХ ЭКСПЕРИМЕНТАЛЬНОГО САЛЬМОНЕЛЛЕЗА

Salmonellosis is a serious pathology of infectious origin, leading to high economic losses in poultry farming. A particular danger of the disease is that infected poultry may be a hidden bacterial carrier, and salmonellosis is also a threat to humans. For preventive, as well as therapeutic measures, antibacterial drugs are used, one of which is a representative of fluoroquinolones – Enrofloxacin. Despite the data on the good antimicrobial effect of the drug, little research has been conducted on its impact on the physiological condition of birds, in particular, the impact of Enrofloxacin on white blood. For the study four groups were formed, from which II and III received Enrofloxacin in doses of 200 mg/l and 100 mg/l, while I and IV – normal drinking water, and groups II, III, IV were experimentally infected with Salmonella infantis culture in the concentration of 30 million COE/0.5 ml. Blood samples were taken on the 1st, 3rd, 5th, 7th and 9th day after the drug withdrawal. The following parameters were studied: blood leukocyte content, leukocyte formula, and dynamics of leukocyte indices. The revealed changes in all experimental groups testify to the ambiguous antibacterial effect of the use of Enrofloxacin, although the use of 200 mg/l dosage, to some extent, was more effective. Nevertheless, on the whole, in spite of the revealed reliable shifts of the studied indicators in chickens of the experimental groups, no negative effect of the preparation on the cockerels' organism was revealed.

The effect of oligopeptides-homologs of the ACTH15-18 fragment on the adaptive response of rat blood on the acute cold stress model

Topicality. One of the important stress-protective mechanisms of oligopeptides – homologs of the ACTH15-18 fragment (under the codes KK-1 and KK-5) found in the previous studies is prevention of the hypothalamic-pituitary-adrenal system dysfunction on the acute cold stress model.Aim. To study the effect of oligopeptides – homologs of the ACTH15-18 fragment on the adaptive response of rat blood on the acute cold stress model.Materials and methods. The hemoglobin content, erythrocyte sedimentation rate, white blood cell count and leukocyte indices were studied in the blood of rats after acute cold stress.Results and discussion. Against the background of the use of peptides compared to the indicators of the control pathology group the hemoglobin content (KK-1 – by 13 % and KK-5 – by 11 %) significantly increased, while the erythrocyte sedimentation rate decreased (KK-1 – by 1.9 times and KK-5 – by 1.7 times). The peptides significantly reduced the manifestations of neutrophilia, lymphopenia and eosinopenia compared to the control pathology. Conclusions. The analysis of the experimental data indicates a significant positive effect of oligopeptides – homologs of the ACTH15-18 fragment on the adaptive response of rat blood. Preventing deep hypothermia the peptides contributed to the elimination of pathological abnormalities in the blood system. This conclusion is supported by their restorative effect on the hemoglobin level, erythrocyte sedimentation rate, white blood cell count and leukocyte indices.

CHANGES IN INDICATORS OF ENDOGENOUS INTOXICATION, IMMUNEINFLAMMATORY REACTION AND ENDOTHELIAL DYSFUNCTION UNDER THE INFLUENCE OF TREATMENT OF PATIENTS WITH ALCOHOLIC LIVER CIRRHOSIS IN COMBINATION WITH OBESITY USING ADEMETHIONINE AND ARGININE GLUTAMATE

The аim of research was to investigate the effect of a complex treatment with ademethionineand arginine glutamate on the state of endogenous intoxication, immune-inflammatoryreaction and endothelial dysfunction in patients with alcoholic liver cirrhosis (ALC) incombination with obesity.Methods. 215 patients, diagnosed with ALC, took part in the study, including 66 womenand 149 men. 109 people had ALC with obesity and 106 people had ALC without obesity.Patients were divided into subgroups depending on the stage of decompensation accordingto Child-Pugh. Depending on the treatment protocol (b protocol – basic therapy, hprotocol – basic therapy in combination with ademethionine and arginine glutamate), allpatients were divided into subgroups.Results. In patients of groups I and II who received the h protocol, at the stage ofcompensation, subcompensation and decompensation, the indicators of sorption capacityof erythrocytes (SCE), leukocyte index of intoxication (LII), high-sensitivity C-reactiveprotein (hs-CRP), tumor necrosis factor alpha (TNFα), asymmetric dimethylarginine(ADMA) and the resistin level significantly improved (p 0.05). At thestage of subcompensation and decompensation in patients of groups I and II, who receivedbasic treatment, SCE, LII, hs-CRP, TNFα, ADMA and the resistin level significantlyworsened (p<0.05).Conclusions. Inclusion in the complex treatment of ademethionine and arginineglutamate for obese patients with ALC helps to reduce the manifestations of endogenousintoxication, immune-inflammatory reaction and endothelial dysfunction.

Gallbladder distension as a triggering link of the reflex influences of afferent pathological impulses

Objective — to investigate correlation between sonographic gallbladder (GB) parameters and clinical characteristics, results of laboratory investigations and structural and functional cardiac parameters in patients with the ischemic heart disease and arterial hypertension.Materials and methods. The GB sizes were estimated in 64 patients with ischemic heart disease (stable and unstable angina pectoris), in 31 patients with arterial hypertension, overweight or obesity, and 18 healthy persons without cardiovascular disorders. A number of cell ratios and integral hematological indices were calculated, in particular index of immunoreactivity, index of inflammation activity, integral index of inflammation, leukocyte index of intoxication, lymphocyte‑granulocyte index, index of leukocyte shift.Results. It has been established that the increase of GB sizes was associated with activation of inflammation and endogenous intoxication syndromes, inhibition of the immune response and cardiovascular risk factors: elongation of heart electric systole, arterial hypertension, increase of body weight. Distension of GB against the background of cardiovascular pathology was accompanied by the decrease of its contractility (according to ejection fraction). The increase of GB sizes together with the stimulation of GB wall mechanoreceptors may act as a trigger of pathological reflex influence, which leads to the viscero‑visceral cardioneuropathy.Conclusions. The obtained data enabled to open a new direction in the strategy of cardiovascular prophylaxis, the reduction of pathological impulses from a distended gallbladder in cardiovascular patients who was undergone timely lithotripsy and low‑invasive cholecystectomy, are the ways to lower the cardiovascular risk, and the improvement of the state of biliary ducts is an important component of the primary cardiovascular prophylaxis.

POSSIBILITIES OF REGULATING STRESS-PROTECTIVE ACTIVITY OF FOOD PRODUCTS TO IMPROVE IMMUNITY UNDER THE CONDITIONS OF THE COVID-19 PANDEMIC

Aim. The paper aims to develop food products that contribute to the resistance of active people to stress factors, especially during the COVID-19 pandemic. Materials and methods. The cohort study was conducted in three parallel groups of volunteers aged from 20 to 40 years (n = 45) who were consistently involved in sports (low-intensity training at least 3 times a week). As food products with stress-protective activity, freeze-dried taxifolin nanoemulsion and whole-wheat flour from wheatgrass were used. As mental and informational stress, media coverage of global and Russian COVID-2019 dynamics was used. To assess the impact of the developed products on stress resistance, a study of health and psychoemotional status (the SF-36 questionnaire) and biochemical blood analysis (leukocyte formula and cortisol) were carried out. Results. The results demonstrated the possibility of using stress-protective food products such as freeze-dried taxifolin nanoemulsion and whole-wheat flour from wheatgrass for improving immunity. The assessment of the psychoemotional status revealed an equally positive effect from the inclusion of taxifolin nanoemulsion and whole-wheat flour from wheatgrass in the composition of food products. Biochemical blood analysis and leukocyte indices revealed a greater positive effect of taxifolin nanoemulsion on the human body, while regular consumption of whole-wheat flour-based products from wheatgrass reduces blood cortisol and increases the body's resistance to stress factors. Conclusion. People involved in low-intensity sports are recommended to consume foods enriched with taxifolin nanoemulsion and whole-wheat flour from wheatgrass to improve their psychoemotional status and resistance to stress factors such as the COVID-19 pandemic.

Application of indirect electrochemical oxidation of blood in a complex of intensive therapy in children with urolithiasis complicated with chronic kidney disease

Objective. To study the efficacy of sodium hypochlorite in children with urolithiasis complicated by chronic kidney disease (CKD).&#x0D; Materials and methods. The results of the use of sodium hypochlorite 0.03-0.06 % in the pre- and postoperative period in 20 patients with urolithiasis complicated by stage IV CKD were analyzed. The indices of endogenous intoxication in venous cubital blood (VСK), mixed venous blood (MVB) flowing to the lungs and arterial blood (AB) flowing from the lungs were studied. Indirect electrochemical oxidation of blood (IEOB) was carried out in 20 patients with stage. For children under 12 years old, we used 0.03 %, and for the older age group from 12 to 18 years old ‒ 0.06 %, the volume of the injected solution did not exceed 1/10 of the patient’s CBV per infusion, at a rate of 40-60 drops/min. We have studied the use of sodium hypochloride (NaCIO) as a component of complex intensive care (CIC) in preoperative preparation in children with urolithiasis complicated by CKD and the effect of sodium hypochloride on detoxification lung function (DLF).&#x0D; Results. There was a significant increase in low molecular weight toxic substances (creatinine, calurea), average mass molecules (AMM), lipid peroxidized products, oligopeptides of a high necrotic body mass (NB), circulating immune complex (CIC), integral indicators of toxicity leukocyte index of intoxication and index of intoxication, as well as a decrease in antioxidant protection, more pronounced at sub- and decompensated stages of DLF impairment in the examined group of patients before treatment in the basin of VKK, VCK and AB. In 7 (35.0 %) patients ‒ 1b compensated, in 13 (65.0 %) ‒ subcompensated stage of impairment.&#x0D; Conclusions. CIC with the use of IEOB in the preoperative preparation by infusion of sodium hypochlorite in this subgroup of patients significantly improved DLF in relation to NB, CIC and high-weight oligopeptides. The use of NaCIO leads to inactivation of the entire spectrum of AMM and large toxic molecular compounds fixed on the membranes of blood corpuscles, and also, ensures the transformation of hydrophobic metabolites into hydrophilic ones, which are actively excreted by excretory organs.

ИЗМЕНЕНИЯ НЕКОТОРЫХ ГЕМАТОЛОГИЧЕСКИХ ТЕСТОВ КАК РЕЗУЛЬТАТ ВОЗДЕЙСТВИЯ ШИРОКОПОЛОСНЫХ ЭЛЕКТРОМАГНИТНЫХ ПОЛЕЙ В ЭКСПЕРИМЕНТЕ

The article presents the results of studying hematological tests of small laboratory animals after exposure to broadband electromagnetic radiation. To achieve this goal, three study groups were formed, each of which included 10 male mice. After exposure, the parameters of the quantitative analysis of leukocytes, leukocyte formula and leukocyte indices of the control, experimental and intact groups were compared. The statistical significance of the revealed differences was determined using the Mann-Whitney test. As a result of the study, when determining the total concentration of leukocytes, statistically significant differences were revealed between the experimental and intact groups, the control and intact groups. During the analysis of the data of the leukocyte formula, no statistically significant differences were revealed, however, in the experimental group, atypical forms of leukocytes with myelodysplasia were revealed. When studying the data of a biochemical blood test and calculating leukocyte indices, no significant differences between the study groups were also revealed.

Increased Stress Resistance of the Offspring Egg Cross Chickens when Using Biologically Active Substances before Hatching.

Application of biologically active compositions, containing colamine, succinic acid, serine, and pyridoxine hydrochloride before hatching decreased hatchery waste and increased hatching rates by 2.65% and egg hatchability by 1.85%. The chicks of the experimental group exhibited a decrease in the intensity of lipid peroxidation, which indicated a greater resistance to stress. This conclusion is additionally based on an increase in the Krebs index by a factor of 1.74 (p < 0.01) and leukocyte intoxication index by 5.53% and a decrease in the lymphocyte-granulocyte index by a factor of 1.75 (p < 0.05) and leukocyte index by 1.67% (p<0.05). The body length of the offspring of the experimental group was by 2.12% less and safety for 60 days of cultivation was 3% higher compared to the control.

FACTORS ASSOCIATED WITH RECURRENT PREGNANCY LOSS IN THE SUBARCTIC REGION: A LITERATURE REVIEW

Mortality rate in the Russian Federation in 2018 exceeded the birth rate again. At the same time, a stable and high frequency of early reproductive losses was noted. Every fifth wanted pregnancy terminates spontaneously before the due date. Living in the subarctic zone is associated with several unfavorable climatic factors. Implementation of the reproductive function in these conditions is influenced by region-specific factors, which have multiple effects on the mechanisms of recurrent pregnancy loss. The literature search was performed using PubMed, Medline, Cyberleninka, Scopus and HAC databases. Assessing the degree of adaptation of women of reproductive age living in the subarctic zone of the Russian Federation helps to assess their psychosomatic status and heart rate variability. Taking into account the adaptation parameters, certain patterns were revealed for women of reproductive age, as well as for each trimester of a normal and pathologically ongoing pregnancy. Particular attention in the assessment of adaptive resources of the body deserve leukocyte indices, by changing the parameters of which it is possible to make a forecast of the ongoing pregnancy. The results of this review are of interest to the clinicians working in the subarctic zone of Russia from the standpoint of personalized approach to prevention and prediction of recurrent pregnancy loss.

THE EFFECT OF ENTEROSORPTION AND INTRATISSULAR ELECTROFORESIS OF ANTIBACTERIAL DRUGS ON PROGRESS OF BURN DISEASE

Summary. The purpose of our research was to explore the appropriateness of combined usage of enterosorption amplification and intratissular electrophoresis (ITE) of antibiotics in cases of substantial thermal burns. The effect of intratissular electrophoresis (ITE) of antibacterial drugs and enterosorption was studied in 22 patients with dermal thermal burns from 28 to 54 % of their body surface. ITE of antibiotics started from the 3–4 day applying the direct current with density 0,03-0,05 mAh/cm² for 60 min. by the «POTOK-1» device.&#x0D; The authors determined the intoxication degree based on the leukocyte index, the number of average mass molecules and blood plasma electrical conductivity. Also, they observed the course of wound process, conducted bacteriological and cytological researches of the burn wounds in dynamics on 1–3, 6–7, 13–14 and 19–21 days of the burn disease progress. It was found that enterosorption usage in the comprehensive treatment of burn disease allows not only to reduce the level of endogenous intoxication but also to provide an indirect optimizing impact on the progressing of the wound process in the zone of thermal damage, stimulate the reparative regeneration. In the cases with extensive burns, combined usage of the enterosorption detoxification by Enterosgel with the ITE of antibacterial drugs contribute more qualitative positive influence on the wound process and burn disease in general.

THE STATE OF THE MOTOR-EVACUATOR FUNCTION OF THE DIGITAL CHANNEL IN PATIENTS AFTER SURGICAL TREATMENT OF ACUTE OBSTRUCTION OF SMALL INTESTINE AND ITS INFLUENCE ON ITS COURSE

Summary. Disruption of the motor-evacuation function of the digestive canal is an integral cause of the adverse course of acute obstruction of the small intestine and as a consequence of high lethality in this pathology.&#x0D; Purpose of the study. To study the motor activity of the gastrointestinal tract in patients with acute obstruction of the small intestine and its effect on the course of the disease in the postoperative period to create effective, sound methods of treatment of these patients.&#x0D; Materials and methods. Complexly after surgery, 221 patients with acute obstruction of the small intestine were examined. Used clinical and instrumental methods for the study of motor-evacuation function of the gastrointestinal tract (electrogastrography, electroenterography, phonoenterography), determined the degree of intoxication syndrome (leukocyte index of intoxication, molecules of medium mass), nonspecific resistance to organism operating-anesthetic index on ASA scale.&#x0D; Results and its discussion. Functional state of the patient with acute obstruction of the small intestine, its comorbidity affects the degree of operativeanesthesiological risk and the state of the motor-evacuation function of the digestive canal. It was found that inhibition of the motor function of the digestive tract in the first three days after surgery led to an increase in the level of intoxication, requiring its mandatory correction.&#x0D; Conclusions. A promising direction in the treatment of patients with acute obstruction of the small intestine is to develop measures that contribute to the preservation, protection and restoration of motility of the digestive tract at all stages of treatment.