Plantlet Length Research Articles

Variability in salinity stress tolerance of potato (Solanum tuberosum L.) varieties using in vitro screening

ABSTRACT Salinity is one of the abiotic stresses that lead to an imbalance in the physiological processes of the plants and also affects potato growth and productivity in maınly semi-arid and growing areas. The accumulation of Na+ and Cl- ions in the cells is very toxic can influence all mechanisms and the enzymatic actions of the plants. In vitro screening of plant genotypes for osmotic stress represents a valuable tool as an alternative to field trials and can be applied based on osmotic stress tolerance. The main goal of this study was to reveal variability in salinity stress tolerance of potato varieties using in vitro screening. Stem cuttings consisting of a single node of different varieties were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of sodium chloride (NaCl) (0, 50, 100 and 150 mM). The differences among the plantlet length, number of branches, number of nodes, number of the leaflet, leaflet width, leaflet length, root length, number of the root, fresh plantlet weight, dry plantlet weight of all varieties were negatively influenced by all NaCl concentrations tested. Microtuberization and stolon growth of the varieties were also completely inhibited at high concentrations (100-150 mM). The Principal components analysis (PCA) was applied to the data matrix (15 morphological characteristics x 12 potato varieties) of the potato varieties. Also, a hierarchical cluster analysis (HCA) was used to identify the possible nearest and similarity of all morphological characteristics analyzed of the potato varieties. In grouping potato varieties, HCA and PCA results were found to be similar. We can speculate about the responses of morphological similarities of the potato varieties against salt stress. We concluded that Innovator and Kennebec are respectively the most salt-tolerant varieties. Hermes was moderately salt-tolerant and microtuberization capacity of Slaney was also high under salt stress conditions.

Effects of Teramnus labialis (L.f.) Spreng seed cryopreservation on subsequent seed and seedling growth and biochemistry

Teramnus labialis (L.f.) Spreng is a legume that plays an important role in agriculture, due to its use as animal feed and its role as an enhancer of soil physicochemical conditions. However, given previous reports on the effects of seed cryopreservation on seedling vigor and biochemistry in a number of species, the present study looked at the effects of T. labialis seed cryopreservation on subsequent seed and seedling [0–28 days post seed exposure to liquid nitrogen (LN)], growth (germination, seedling length and fresh and dry mass) and biochemistry (chlorophyll, aldehyde, phenolic and protein levels). The seeds were intact in terms of macrostructure after exposure to LN, however, there was a significant (3.5-fold) increase in electrolyte leakage during imbibition. Seedling emergence was also improved by cryostorage during the 1st week of culture (4.1-fold increase) and at 28 days (2.5-fold increase). Consequently, seedling growth (in terms of plantlet length, and fresh and dry mass) was superior in seedlings arising from cryopreserved seed, but this stimulatory effect was more evident at 14 than 28 days of culture. An increase in malondialdehyde levels in cryopreserved seeds is most likely a consequence of damage to the external seed structures following cooling and rewarming, while the rise in cell wall-linked phenolics and aldehydes in roots of seedlings produced from cryopreserved seeds could be linked to water and nutrient stress brought about by greater root growth.

An Efficient Method for Acclimatization:<i>in vitro</i> Hardening of Tissue Culture-Raised Tea Plants (<i>Camellia sinensis</i> (l.) O. Kuntze)

In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by root induction of micropropagated tea shoots ( Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.

Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium spectabile orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1. The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves. Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets. BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture

Effect of Micronutrients on The Two Qatari Date Palm Cultivars Shishi and Lulu Through in Vitro Technic

Plant tissue responds to nutrient media due to the plant cultivars genetic diversity. The objective of this research is to determine the effects of micronutrients on date palm growth and which of these micronutrients are critical for improving growth combined with Paclobutrazol or without on in vitro of elongation stage. In vitro growth of two cultivars were determined by varying five treatments that included minor salts (EDTA-chelated iron, CuSo4,) alone or combined with Paclobutrazol. The effects of these five treatments on plantlet length, trunk thickness, rooting number and Chlorophyll A, B was investigated. The results obtained after three months showed that, increasing copper sulphate on MS elongation media combined with Paclobutrazol was more effective for increasing the trunk thickness as well as rooting number compared with normal MS level. While poor response was found when Fe was increased in the chelated form of Fe-EDTA on MS elongation media. For cultivars, Shishi gave the highest plantlets length as well as root number. The maximum content of chlorophylls A and B were observed when copper sulphate was increased on MS elongation media with cultivar Lulu. The survival rate after 3 months was related to the medium used during the elongation phase, the survival of plantlets reached to 98.66% with cultivar lulu at the acclimatization stage.

PERTUMBUHAN KULTUR TUNAS AKSILAR KENTANG (Solanum tuberosum L.) DENGAN PENAMBAHAN SUPER FOSFAT DAN KNO3 PADA MEDIA AB MIX SECARA IN VITRO

Murashige&Skoog-based medium Potatoes are one commodity that has the potential to be developed as a resource in the context of food diversification, farmers' income riser, non fossil export commodities and raw materials for processing industry. The objective of this research was to find out the effect of SP-36 fertilizer, KNO3 fertilizer, as well as the interaction between the two fertilizers on the growth of potato nodal culture on AB Mix media in vitro. The results of this study are expected to provide economical potato tissue culture media development. This research used factorial complete randomized design. The treatment were SP-36 concentration: 0 ppm; 50 ppm; 100 ppm; and 200 ppm, in combination with KNO3 concentration: 0 ppm; 100 ppm; 200 ppm; 400 ppm; and 600 ppm, The variables observed included number of leaves, leaf color, length of plantlets, fresh weight of plantlets and percentage of plantlets growth. Based on the results of the analysis of variance (ANOVA) F. Calculate < F. Table with the average success of plantlet growth between 87.5-100%. In addition, there are four types of leaf color produced, namely the color of yellowish green, pale green leaves, green, and dark green. Research showed that the interaction between SP-36 fertilizer and KNO3 fertilizer on AB Mix media had no significant effect on all observed variables.

Physiological Effect of Potato Genotypes and Salicylic Acid on Plantlets Growth and Microtuber Production under Salt Stress

This study was conducted to investigate the effectiveness of SA (10 and 100 μmol) for induction of salinity tolerance of two potato genotypes “Proventa” and line “97-980” during vegetative and micro-tuberization stages. Results showed that decreasing of plantlet weight and length as well as microtuber weight under saline conditions differed according to genotypes and SA concentration. Although line “97-980” had low percentage of decreasing in weight and length of plantlets compared to “Proventa” under saline condition, Proventa cv. Gave high microtubers weight. Line 97-980 recorded high reducing sugars, phenolics, proline, amino acids and proteins as well as high activity of peroxidase (POD). On contrary, “Proventa” had high concentration of total chlorophylls and high activity of superoxide dismutase(SOD) and catalase (CAT).The decrease in plantlets growth was lower in line 97-980 compared to” Proventa” under low concentration of SA. Microtubers weight in “Proventa” was higher with addition of 10 μmol through increment in most investigated biochemical compounds and high antioxidants enzymes activity. It may be concluded that high content of reducing sugars, phenolics, proline, amino acids, proteins as well as high activity of POD could work as selectable markers for in vitro potato tolerance to salinity. Addition of low concentration of SA had beneficial effect on physiological

Nutritional requirements for germination and in vitro development of three Orchidaceceae species in the southern Brazilian Amazon

Tissue culture is an alternative form of producing healthy, vigorous and regular plants on a large scale. The purpose of this study was to evaluate the most efficient culture medium for in vitro plantlet germination and development of three Orchidaceae species. Seeds disinfested of three species were dispersed in distilled water and dripped into basic Murashige and Skoog (MS) medium. The experimental design was completely randomized in a factorial 3 x 4 (three species x four culture media), with 5 replications. Four treatments were established: (1) full-strength MS medium, with the full nutrient concentration (MSØ), (2) full-strength MS medium plus 0.3% activated charcoal (MSØ ACh), (3) half- strength MS medium (½ MS) and (4) half- strength MS medium with 0.3 % activated charcoal (½ MS ACh). Germination was evaluated after 15, 20, 25, 30, and 60 days. The shoot height, leaf number and length, root number and length of plantlets of the three studied species were assessed. In A. variegata, 73% germinated after 60 days in ½ MS ACh medium. In the same period, 100% of E. viparum and S. gloriosa seeds germinated in MSØ ACh medium. The plant height, leaf number and length, root number and length were significantly higher for the species A. variegata and E. viviparum in MSØ ACh medium. The culture media ½ MS and MSØ with addition of activated charcoal favored in vitro germination for the three orchid species of this study.

Hydrothermal Carbonization of Municipal Woody and Herbaceous Prunings: Hydrochar Valorisation as Soil Amendment and Growth Medium for Horticulture

In this study, we investigate the suitability of hydrochar, produced at industrial scale by hydrothermal carbonization of municipal woody and herbaceous prunings, to be used as soil amendment and peat substitute in organic growth medium for horticulture. Fresh hydrochar and the products of two different hydrochar post-treatments (i.e., washing and aging) were compared in terms of potential phytotoxicity throughout physicochemical characterization and germination tests, performed with a sensitive species (Lactuca sativa). The results showed that the fresh hydrochar obtained from municipal green wastes complies with the Italian regulated parameters for the use as soil amendment. Moreover, hydrochar exhibits biological activity and a high content in organic C, Ca, and other micronutrients (Mg, Zn, Cu, Na, Cl). On the other hand, post-treatments are needed before application of hydrochar as peat substitute in potting mix, since appreciable phytotoxic effects on lettuce seed germination and radicle length of plantlets were observed (e.g., germination percentage of 56% and 54%, with 5 and 10 wt % of hydrochar in the blend, respectively). The inhibition of germination could be mainly attributed to the presence of polyphenols (tannins) and volatile fatty acids, which were most effectively removed through the aging post-treatment.

Tagetes erecta (marigold), a phytoremediant for Ni- and Pb-contaminated area: a hydroponic analysis and factors involved

With increasing industrialization and increasing population, excess accumulation of heavy metals such as Cd2+, Cr6+, Cu2+, Ni2+, Pb2+ and Zn2+ in soils have caused the disruption of the terrestrial ecosystem. Different chemical, physical and biological techniques are being explored for the remediation and restoration of such heavy metal-polluted soil. This study aims to evaluate the tolerance capacity of Tagetes erecta for heavy metals Pb2+ and Ni2+ with the objective of using this plant for phytoremediation of heavy metal-polluted soil. The plant is found to tolerate up to 200 mmol of both the test heavy metals. Dry weight, root length, proline content and antioxidant activity increased significantly in all the treatments, whereas shoot length, wet weight and chlorophyll content of plantlets were adversely affected after 21 days of metal treatment in hydroponic culture condition. This study suggests that Tagetes erecta has developed a defense mechanism against heavy metal stress, suggesting its acceptability for phytoremediation in soil conservation.

Growth Responses of Potato Plantlets Cultured In Vitro under Different Colors Light-Emitting Diodes (LEDs)

This exploration work target was to upgrade the micropropagation of potato cultivars through the utilizationof new light sources in the development rooms. The experiment consisted of two potato cultivars (Diamant and agria),and five light sources (white, blue, green and red LEDs; and white fluorescent lights). The explants comprised of nodalsegments containing one bud, segregated from plantlets developed in vitro. The experimental design was completelyrandomized arranged in a 2x5 factorial, with 4 replications. Each experimental unit consisted of a flask with fiveexplants. Three 28-day successive subcultures were done in MS medium and incubated under controlled conditions(temperature = 25+2oC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). Toward the finish of everysubculture, plantlet length, number of nodes per plantlet, internode length and plantlet fresh weight were assessed. Afterthe second subculture, differences were found among the two potato cultivars developed in vitro: Agria performedbetter than Diamant in most plantlet growth parameters. Different light sources influenced the potato plantletdevelopment: red and green LEDs were the most and least prescribed for plantlet advancement respectively, in view ofthe after effects of bud number per plantlet, plantlet length. Photosynthetic pigment contents were significantly differentunder the different light treatments. Chlorophyll a, b and carotenoids were higher in plantlets grown under Red, and lessin plantlets under green LED light.

Carbon nanotubes impact on date palm in vitro cultures

Among the recent line of technological innovations, nanotechnology takes a promising position in agriculture and food production. Nanotechnology permits definite advances in agricultural research, such as reproductive science and technology. This investigation is interested in studying the in vitro effect of carbon nanotubes (CNTs) on callus, embryogenesis, embryo germination and elongation as well as rooting stage of date palm. Carbon nanotubes concentrations were investigated as 0.0, 0.05 and 0.1 mg/l. Results showed that CNTs affect all stages of micropropagation of date palm. Callus fresh weight showed the optimum value at 0.05 mg/l. In embryogenesis stage, CNTs decreased the number of embryos compared with the control while, increased number of germinated embryos and root number. Carbon nanotubes gave a significant enhancement for shoot length and leaf number in elongation stage. Similarly, it enhanced root number, root length, plantlet length and hairy roots. Chemical analysis as chlorophyll a, b, carotenoids, flavonoids, antioxidant enzymes and nutrients concentration and uptake were determined.

Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

In vitro regeneration protocol development via callus formation from stem explant of tomato

The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (21.00) of roots/plantlet, length (7.676 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the stem explants. The results of the current study showed significant increase in the growth of callus of Solanumlycopersicon Mill. Indicating a good efficiency of the optimized media composition and the experimental model used in comparison to other studies of similar nature.Asian J. Med. Biol. Res. December 2015, 1(3): 589-599

Umedecimento do substrato e temperatura na germinação e vigor de sementes de pitaya

Knowledge of appropriate conditions of water and temperature is very important for the metabolic activities involved in the germination process. However, the Rules for Testing Seeds do not indicate the volume of water and the ideal temperature for pitaya germination. The study was carried out to evaluate different water volumes in the substrate and temperatures on seeds germination and vigor of pitaya. The experimental design was completely randomized in a factorial 5 x 3, with treatments consisted of five water volumes in the substrate and three temperatures. The used water volumes was equivalent to 1.5; 2.0; 2.5; 3.0 and 3.5 times the dry weight of the substrate. Were tested at constant temperatures of 20, 25 and 30 °C. The parameters evaluated were: germination, first count of germination, emergence speed index, length and dry mass of plantlets. The temperature of 25 °C and a water volume in the range of 2.5 to 3.5 times the weight of substrate is the most suitable combination for doing germination tests and evaluating the vigor of pitaya seeds. The temperature of 20 °C and the volume of water 1.5 times the weight of the substrate inhibited germination of seeds

English

This research work objective was to optimize the micropropagation of potato cultivars through the use of new light sources in the growth rooms. Treatments consisted of three potato cultivars (Asterix, Catucha and Macaca), and five light sources (blue, green and red LEDs; Growlux and white fluorescent lamps). The explants consisted of nodal segments containing one bud, isolated from plantlets grown in vitro . The experimental design was completely randomized arranged in a 3x5 factorial, with eight replications. Each experimental unity consisted of a flask with five explants. Three 28-day consecutive subcultures were carried out in MS semi-solid medium, in growth-room under controlled conditions (temperature = 25 + 2 oC; photoperiod = 16 hours; light intensity = 20 μmol m -2 s -1 ). At the end of each subculture, the bud number per plantlet, plantlet length and internode length were evaluated. After the third subculture, the concentrations of carotenoids and a- and b- chlorophylls were also determined. Different micropropagation efficiencies were found among potato cultivars grown in vitro conditions: ‘Macaca’ was the most and ‘Catucha’ the least responsive cultivar. The growth room light sources differently affected the potato plantlet development: red and green LEDs were the most and least recommended for plantlet development, based on the results of bud number per plantlet, plantlet length, and leaflet concentrations of a- and b- chlorophylls and carotenoids.

Cryopreservation of Seeds and Embryos of Jatropha curcas L.

Jatropha curcas is a species with a variety of uses. It is grown primarily for oil for biodiesel, but also has agronomic and medicinal applications. Two methods were evaluated for cryopreservation of seeds and zygotic embryos of J. curcas: desiccation followed by rapid immersion of seeds and embryos in liquid nitrogen (LN, -196°C), and vitrification of zygotic embryos. Prior to cryo-preservation, seeds were manually scarified and the moisture content (MC) of seeds and embryos was determined. Explants were disinfected after cryopreservation. Seed germination after LN exposure was 100%. Plantlet development was better in sand substrate than that in vitro. Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments. Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation. Zygotic embryos subjected to the vitrification procedure did not withstand LN exposure. Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.

Variability of Young Maize Plants Traits in a Set of Local Populations

Taking into account the importance of root and coleoptile and mesocotyl length of maize young plants, we considered appropriate to analyse the variability of these traits in a set of maize local populations. The root system development, the mesocotyl and the coleoptile were studied in a number of 41 local populations at The Maize Breeding Department of ARDS Turda. The average length was 28.42 cm for embrionary roots, 17.37 cm for primary roots, 1.43 cm for mesocotyl, 3.50 cm for coleoptile and 4.92 for plantlets. The variability was low for embrionary roots, coleoptile and plantlets length and medium for primary roots and mesocotyl length. A correlation was noticed between the length of mesocotyl and the length of coleoptile and plantlets. The size of roots, mesocotyl and coleoptile is influenced by the area where the local population is formed. Local populations with high root and coleoptile length such as Tulca, Lapusneac and Biertan can be used for obtaining some valuable inbred lines of maize.

Supression of seed borne Cladosporium herbarum on common bean seed by Trichoderma harzianum and promotion of seedling development

Trichoderma harzianum isolates have been broadly used for biocontrol of plant diseases caused by fungi. Cladosporium herbarum is a common saprophyte and seed borne fungus, which is easy to manipulate under controlled conditions. It was chosen as a model to test the effectivity of seed treatments with T. harzianum. Common bean seeds (cv. Perola) contaminated with C. herbarum were treated with conidial suspension (CS) and autoclaved filtrate (AF) of five isolates of T. harzianum and subsequently submitted to health and germination tests. The proportion of normal seedlings formed, the length of roots, hypocotyls and leaves, and total plantlet length, total plantlet biomass, root mass ratio (RMR), stem mass ratio (SMR), leaf mass ratio (LMR), aerial part/root system ratio (AP/RS) and leaf area were also evaluated. Isolates CEN289 and CEN290 (CS and AF) provided 66 to 77% of supression of C. herbarum on seeds and a higher number of normal seedlings as compared with control. It also yielded a higher total biomass of plantlets. Moreover treatment with isolates CEN289 and CEN290 increased root and stem length in the experiments with CS. Such results indicate the potential of T. harzianum for seed treatment and suggest that it should be further tested as control for seed borne fungal diseases and as a plant growth promoter. The better performance found for CEN289 and CEN290 confirms the variability in terms of biocontrol activity among strains of T. harzianum.

Identification of the effect of different levels of activated charcoal and sucrose on multiplication shoots of date palm phenixdactylifera L.C.v. sufedy in vitro

This study was conducted at the Laboratory of Plant Tissue Culture of the Palm Research Center / University of Basra from March 2010 to September 2011. The aim is to determine the effect of the use of activated charcoal and sucrose on the multiplication of shoots, rate of elongation, average number of roots and length of plantlets of date palms C.v Elsafada in vitro. Activated charcoal concentrations were zero, 0.5, 0.75, 1, and 1.25 g/l. Those of sucrose were 20 (control), 35, 50, 65, and 80 (g/l). Nutritional media were prepared with some other chemical substances as well as 3 mg/l Naphthalene acetic and 10 mg/l 2-Isopentenyl adenine (2iP). Transplants were incubated under illumination intensity lighting of 1000 Lux for 16 h a day and a temperature of 27 ± 1°C. A re-culture was done every four weeks. The use of 0.75 g/l of activated charcoal caused a significant improvement in the percentages of shoots multiplication (9.6%), rate of elongation (7.3 cm), number of roots (4.6 root/plantlets) and their length. However, the 0.5 concentration exceeded others in root length (5.3 cm). Use of 65 g/l sucrose increased the multiplication ratio (5.3%), elongation (7 cm) and number of roots (4.6 root/plantlet). While use of 80 g/l led to higher length of roots (5.8 cm) compared to the other concentrations. Results also showed that the use of 0.5 g/l activated charcoal and 65 g/l sucrose improved the multiplication ratio (9.2%), elongation (8.1 cm) and average number of roots (5.3 root/plantlet). Furthermore, the treatment of 0.75 g/l activated charcoal and 65 g/l sucrose significantly increased the average of root length (6.9 cm) compared to other treatments. Key words: Date palm, elongation, activated charcoal.