Abstract
The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium spectabile orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1. The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves. Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets. BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture
Highlights
17 | Paspalum : JurnalIlmiahPertanianVolume 7 N o 1 Maret 2 0 1 9 ditumbuhkan selama 12 minggu pada media dasar Murashige and Skoog setengah konsentrasi disertai dengan aplikasi NAA dan BAP sesuai masing-masing perlakuan
This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty
The experiment was conducted from June 2017 until September 2017
Summary
Metode penelitian ini menggunakan metode eksperimen yang dilaksanakan di Laboratorium Budidaya in vitro Fakultas Pertanian Universitas Winaya Mukti, Tanjungsari Kabupaten Sumedang. Percobaan dilaksanakan dari bulan Juni sampai dengan September 2017. Bahan eksplan yang digunakan berupa protokorm anggrek D. Spectabile masih berbentuk bulatan hijau yang sedikit tumbuh daun (primordia daun), dan sudah berumur 3 bulan sejak penanaman dari biji. Bahan-bahan lain yang digunakan antara lain gula putih, agar-agar bening, arang aktif, aquades, 0,1 % NaOH atau. 0,1 % HCl, clorox, alkohol 70 %, NAA dan BAP, kapas, karet gelang, air, alumunium foil, plastik bening. Alat yang digunakan antara lain : petridis, beakerglass,batang pengaduk, sikat botol, kompor, panci, sinduk ukuran sedang, corong kaca, pipet ukur, bola pipet, botol kaca, skalpel blade, gunting, pinset, enkas, autoclave, oven, timbangan analitik, rak kultur inkubasi dan lampu TL
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
